The majority of freshly sorted SIV-specific CD8+ T cells cannot suppress viral replication in SIV-

infected macrophagesLara Vojnov, PhD

Department of Pathology and Laboratory MedicineUniversity of Wisconsin-Madison

Madison, WI, USA

Evidence of HIV/SIV infection of macrophages

Zhang et al. Science 1999

Veazey et al. Science 1998

In vivo SIVmac239 infection of macrophages in intestinal

lymphoid nodule

CD68+ macrophages comprise 10% of the

SIVmac251 cell population in vivo

Evidence of HIV/SIV infection of macrophages

Zhang et al. Science 1999

Veazey et al. Science 1998

PNAS 2001

SAMHD1 inhibits HIV infection of macrophages without Vpx

Hypothesis

Macrophages play an important role in HIV/SIV infection as a

viral reservoir resistant to CTL-mediated lysis.

SIV-specific CD8+ T cells suppress viral replication in SIV-infected CD4+ T cells

MHC matchedinfected targets

MHC mismatchedinfected targets

SIV-specific CD8+ T cells poorly suppress viral replication in SIV-infected macrophages

MHC matchedinfected targets

MHC mismatchedinfected targets

CD4+ T cells vs macrophages

SIV-specific CD8+ T cells poorly suppress viral replication in SIV-infected macrophages

32% 88%

SIV-specific CD8+ T cells poorly suppress viral replication in SIV-infected macrophages

3% 30%

Bulk CD8+ T cells suppress viral replication in SIV-infected CD4+ T cells

Bulk CD8+ T cells poorly suppress viral replication in SIV-infected macrophages

Time-course of SIV-infected elite controllers

AcknowledgementsNational Institutes of Health

Jorge AlmeidaDanny Douek

NEPRCR. Paul Johnson

FIOCRUZ – Rio De JaneiroMyrna Bonaldo

Marlon Veloso de SantanaPatricia Costa Neves

Ricardo Galler

University of Wisconsin-MadisonMauricio MartinsAlexander Bean

Jonah SachaHolly Kolar

Nancy WilsonBenjamin BurwitzDavid O’Connor

University of MiamiDavid WatkinsAnn Dauphin

Mario Stevenson