Laboratory of bio cybernetics

Lea Retelj, Ajda Bicek, Matevz Leskovsek, Jasna Krmelj, Tadej Kotnik, Dusa Hodzic, Marko Usaj, Gorazd Pucihar, Alenka Macek Lebar, Samo Mahnic, Barbara Mali, Tomaz Jarm, Jaka Cemazar, Selma Corovic, Denis Pavliha, Bor Kos, Masa Kanduser, Natasa Pavselj, Janez Zigon, Ivan Pavlovic, Stanislav Rebersek,

David Slavic, Melvin Essone, Jure Jelenc, Matej Rebersek, Tina Batista Napotnik, Mitja Gliha, Sasa Haberl, Peter Kramar, Igor Marjanovic, Barbara Zorec, Matej Kranjc, Damijan Miklavcic.

(Missing from the photo: Karel Flisar, Marija Marcan, Andraz Polak)

http://lbk.fe.uni-lj.si

http://lbk.fe.uni-lj.si/

Applications of Electroporation

reversible

irreversible Cell death

Introducing small molecules

Introducing largemolecules

Cell fusion

Protein insertion in the plasma membrane

Exposure toelectric field

Extraction of molecules

EP

+ E -

Jarm, Cemazar, Miklavcic, Sersa.Expert Rev. Anticancer Ther. 10: 729–746, 2010

ECT day 0

10 weeks after ECTselectivity of ECT

Julie Gehl. Ugeskrift for Laeger 167(34): 3156-9, 2005.

Mode of operation in Laboratory of Biocybernetics

Capacities and experties• 2 cell culture labs• 1 cell culture lab safety

level 2• 1 microbiology cell lab• Skin ex vivo lab; lipid

bilayer• Electronic workshop• Impedance and

temperature measurements

• Modelling (biological systems, electronic circuitry, multiphysics, multiscale…)

• Cell electroporation– Lipid bilayer– Vesicles (GUV, SUV,…)– Cells (attached, suspension, in

matrigel…)– Cells: single, clusteres– Non-excitable, excitable

• Skin ex vivo• Extraction of intracellular

components from cells• Dielectrophoresis for cell

separation• Bioimpedance

measurement; electrical characterisation

See also: www.electroporation.net

Damijan MiklavčičUniversity of LjubljanaFaculty of Electrical EngineeringLjubljana, SLOVENIA

http://lbk.fe.uni-lj.si

http://www.electroporation.net/http://lbk.fe.uni-lj.si/

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