Laboratory Filtration Products · 10,000 MWCO 25 VS02H01 10,000 MWCO 100 VS02H02 30,000 MWCO 25...

Laboratory Filtration Products

Laboratory Filtration Products2

Ultrafiltration

9 Protein Concentration

36 DNA Concentration

42 Endotoxin Concentration

44 Protein Purification

50 Virus Purification and Concentration

Filtration Devices

60 Minisart® Syringe Filters

76 Sartolab® P20 and Sartolab® P20 Plus

79 Sartolab® 150v

81 Sartolab® RF | BT

84 Chemical Compatibility

Basic Filtration

91 Filter Papers

95 Glass and Quartz Microfiber Filters

98 Membrane Filters

106 Blotting | Chromatography Papers & Blotting Membranes

108 Filtration Equipment

118 Combisart® Manifolds

Filtration and ultrafiltration are essential process steps in nearly all environmental, chemistry and bioscientific laboratory applications.

Sartorius supplies a wide range of individual filter papers, microporous membranes, filtration devices, ultrafiltration units and protein purification devices to suit these applications. This catalog provides a condensed overview of the Sartorius Lab Filtration product range. Please contact us directly for specialty catalogs – available for in-depth technical information.

Table of Contents

XXX4 Ultrafiltration

UltrafiltrationUltrafiltrationIntroduction 5

Protein Concentration 8 Vivaspin® 500

9 Vivaspin® 2

11 Centrisart® I

12 Vivaspin® Turbo 4

13 Vivaspin® 6

14 Vivaspin® 15R

15 Vivaspin® Turbo 15

16 Vivaspin® 20

18 Vivaclear Centrifugal Filters

19 Vivacell 70

22 Vivacell 100

24 Vivacell 250

26 Vivaflow® 50

28 Vivaflow® 50R

30 Vivaflow® 200

32 Vivapore® Solvent Absorption Concentrators

34 Ultrafiltration Membrane Filters

DNA Concentration37 Vivacon® 500

40 Vivacon® 2

Endotoxin Concentration43 Vivaspin® Endotest

Protein Purification45 Vivapure® Ion Exchange Protein Purification Products

47 Vivapure® Mini and Maxiprep Purification Kits

Virus Purification and Concentration51 Vivapure® Virus Purification and Concentration Kits

52 Adenovirus Purification with AdenoPACK Kits

54 Lentivirus Purification with LentiSELECT Kit

UltrafiltrationTable of Contents

6 Ultrafiltration Introduction

Ultrafiltration is a convective process using anisotropic semi-permeable membranes to separate macromolecular species and solvents – primarily on the basis of size. By allowing solvents and salts to pass the ultrafiltration membrane while macromolecules are hindered to pass it, they are being concentrated. However, ultrafiltration can also be applied in solvent exchange applications. Multiple concentration and refilling steps will progressively lead to a buffer exchange, replacing lengthy techniques like dialysis. Although ultrafiltration is not a standard method for separating and fractionating macromolecules, it can be used as such if the macromolecules differ at least 10 times in size. Ultrafiltration is a gentle, non-denaturing method that is more efficient and flexible than other processes.

Ultrafiltration MethodsSartorius offers you a comprehensive range of ultra filtration process methods for the concentration of your biological samples. Below is a guide to selecting the most suitable one as this decision depends on your sample volume and the available equipment, as well as your desired filtration speed and process control.

– Centrifugal Concentration (100 µl to 100 ml Starting Volume) Centrifugation provides the vector to clear solvent and micro-molecules through the ultrafiltration membrane and into a filtrate container positioned below. This is a gentle process that is quick to set up and offers fast filtration speeds for most solutions. Sartorius offers seven Vivaspin® devices and two Vivacell® and Centrosart® I for protein concentration, as well as Vivacon® devices for DNA and peptide concentration | fractionation.

– Pressure Ultrafiltration (5 ml to 250 ml Starting Volume) Pressurized air or inert gas provide the filtration vector for pressure filtration. For the fastest filtration results, pressurized Vivacell® products can be placed on an orbital laboratory shaker. Agitation prevents macro molecules from polarizing on the membrane surface and thus reducing filtration speed. Vivaspin® 20, Vivacell® 70, Vivacell® 100 and Vivacell® 250 can be run with gas pressure. Pressure-fugation is a unique Sartorius method combining gas pressure with centrifugation. This is the fastest of all methods,

7UltrafiltrationIntroduction

providing process times that are typically 30 to 50 percent faster than centrifugation. You can use Vivaspin® 20 and Vivacell® 70 for pressure- fugation (5 to 50 ml starting volumes).

– Crossflow (Tangential Flow) (100 ml to 5l Starting Volumes) The sample is pumped across an ultrafiltration membrane and then returned to the original reservoir by building up pressure at the outlet of the device. The solution is progressively concentrated as solvent and micro-molecules pass through the membrane into a separate filtrate vessel. Reusable Vivaflow® 50R and Vivaflow® 200, as well as disposable Vivaflow® 50, are offered for your dedicated laboratory crossflow filtration.

– Static Absorption (3 ml to 20 ml Starting Volume) This technique uses an absorbent cellulose pad mounted behind the ultrafiltration membrane to draw solvents and micromolecules through the membrane. The retained macromolecules thus concentrate at the bottom of the sample container. You will not need any additional equipment. These devices are ideal for clinical applications like urine concentration prior to further analysis. Both Vivapore® 5 and Vivapore® 20 offer this procedure.

Typical Applications for Ultrafiltration – Concentration | desalting of proteins, enzymes, DNA, monoclonal

antibodies, immunoglobulins, viruses and nanoparticles– Bence Jones Protein concentration from urine samples prior to capillary

electrophoresis– Forensic DNA sample concentration prior to sequencing reaction– FASP (filter-based sample preparation) – peptide fractionation– Free drug | hormone assays– Removal of primers from PCR amplified DNA– Removal of labeled amino acids and nucleotides– Deproteinization of samples– General purpose laboratory concentration and desalting of proteins,

enzymes, DNA, biomolecules, viruses, antibodies and immunoglobulins

Process OptimizationWhen the highest recoveries are crucial, particularly with solute quantities in the microgram range, Sartorius recommends considering the following tips for optimal ultrafiltration results:– Select the lowest MWCO membrane that suits your application. For the

highest recovery, choose a membrane MWCO which is at least half of the molecular weight of the solute to be retained.

– Avoid over-concentration. The smaller the final concentrate volume, the more difficult it is to achieve complete recovery. If feasible, rinse the device with one or more drops of buffer after the first concentration cycle and then recover it again.

– Pretreat the device overnight in distilled water with a passivation solution such as 5% SDS, Tween 20 or Triton X. Rinse thoroughly before use.

8 Ultrafiltration Protein Concentration

Vivaspin® 500

100 µl to 500 µl SamplesVivaspin® 500 µl centrifugal filter units offer a simple, one-step procedure for sample preparation. They can effectively be used in fixed-angle rotors accepting 2.2 ml centrifuge tubes.

The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimizes membrane fouling and provides high-speed concentrations – even with particle-laden solutions.

50

11

SpecificationsVivaspin® 500Concentrator capacity Swing-bucket rotor

Fixed-angle rotordo not use500 µl

Dimensions Total lengthWidthActive membrane areaHold-up volume, membrane and supportDead-stop volume

50 mm11 mm0.5 cm2

< 5 µl5 µl

Materials of construction BodyFiltrate vesselConcentrator capMembrane

PolycarbonatePolypropylenePolycarbonatePolyethersulfone

Ordering InformationVivaspin® 500 Polyethersulfone Qty./Pkg. Prod. No.3,000 MWCO 25 VS01913,000 MWCO 100 VS01925,000 MWCO 25 VS01115,000 MWCO 100 VS011210,000 MWCO 25 VS010110,000 MWCO 100 VS010230,000 MWCO 25 VS012130,000 MWCO 100 VS012250,000 MWCO 25 VS013150,000 MWCO 100 VS0132100,000 MWCO 25 VS0141100,000 MWCO 100 VS0142300,000 MWCO 25 VS0151300,000 MWCO 100 VS01521,000,000 MWCO 25 VS01611,000,000 MWCO 100 VS01620.2 µm 25 VS01710.2 µm 100 VS0172Starter pack(5 of each 5 k, 10 k, 30 k, 50 k, 100 k)

25 VS01S1

9UltrafiltrationProtein Concentration

Vivaspin® 2 Choice of Membranes

0.4 ml to 2 ml SamplesThe Vivaspin® 2 bridges the gap between the 500 µl and 4 ml centrifugal concentrators. This device combines the speed of the classic Vivaspin® products with low internal surface and membrane area for superior recoveries from very dilute solutions.

Available with a choice of PES, Cellulose Triacetate and Hydrosart® membranes, Vivaspin® 2 offers the highest flexibility for process optimization.

Also unique to the Vivaspin® 2 is the choice of directly pipetting the concentrate from the dead-stop pocket built into the bottom of the concentrator or alternatively reverse spinning into the concentrate recovery cap, which can then be sealed for storage. Both methods result in nearly total concentrate recoveries.

20 l

0.1

0.2

0.5

1.0

1.5

2.0

10,000MWCO

126

17


Fixed-angle rotor3 ml2 ml

Dimensions Total lengthWidthActive membrane areaHold-up volume of membraneDead-stop volume

126 mm17 mm1.2 cm2

< 10 µl8 µl


PolycarbonatePolycarbonatePolycarbonate PES, CTA, HY

Ordering InformationVivaspin® 2 Polyethersulfone Qty./Pkg. Prod. No.3,000 MWCO 25 VS02913,000 MWCO 100 VS02925,000 MWCO 25 VS02115,000 MWCO 100 VS021210,000 MWCO 25 VS020110,000 MWCO 100 VS020230,000 MWCO 25 VS022130,000 MWCO 100 VS022250,000 MWCO 25 VS023150,000 MWCO 100 VS0232100,000 MWCO 25 VS0241100,000 MWCO 100 VS0242300,000 MWCO 25 VS0251300,000 MWCO 100 VS02521,000,000 MWCO 25 VS02611,000,000 MWCO 100 VS02620.2 µm 25 VS02710.2 µm 100 VS0272Starter pack (5 of each 5 k, 10 k, 30 k, 50 k, 100 k) 25 VS02S1


Vivaspin® 2 Cellulose Triacetate Qty./Pkg. Prod. No.5,000 MWCO 25 VS02U15,000 MWCO 100 VS02U210,000 MWCO 25 VS02V110,000 MWCO 100 VS02V220,000 MWCO 25 VS02X120,000 MWCO 100 VS02X2

Vivaspin® 2 Hydrosart® Qty./Pkg. Prod. No.2,000 MWCO 25 VS02H912,000 MWCO 100 VS02H925,000 MWCO 25 VS02H115,000 MWCO 100 VS02H1210,000 MWCO 25 VS02H0110,000 MWCO 100 VS02H0230,000 MWCO 25 VS02H2130,000 MWCO 100 VS02H22

Ordering Tips– Choose a membrane pore size at least 50% smaller than the size of the molecule

to be retained.– It is usually best to select polyethersulfone membranes to achieve the

fastest concentrations.– Usually choose cellulose triacetate for protein removal or ultrafiltrate recovery.– Usually choose Hydrosart® membranes for the highest recovery with Ig fractions.

Filtrate and concentrate can be sealed for storage

Direct pipette recovery or choice of reverse spinning concentrate into sample cap

PES, CTA, or Hydrosart® membranes; Filtrate container fits standard 15 ml tube carriers

Integral deadstop avoids risk of concentrating to dryness


Centrisart® I

Centrisart® I* is a ready-to-use unit for small-volume, centrifugal ultrafiltration to separate proteins from low molecular weight substances in biological samples.

Centrisart® I features a unique design that enables ultrafiltration in the direction opposite to centrifugal force. This is so effective in preventing premature blockage of the filter that even whole blood samples can be deproteinized.

ReferencesP. Nebinger and M. Koel:Determination of acyclovir by ultrafiltration and high-performance liquid chromatography.J. Chromatography 619, 342-344 (1993)

F. da Fonseca-Wollheim, K.-G. Heinze, K. Lomsky and H. Schreiner:Serum ultrafiltration for the elimination of endogenous interfering substances in creatinine determination.J. Clin. Chem. Clin. Biochem. 26, 523-525 (1988)

The ultrafiltrate is collected in the floater insert tube, where it is readily accessible without removing the tube.

Centrisart® I is ideal for the following applications:– Drug binding studies– Determination of metabolites in serum– Protein removal from blood samples– Cleaning of liposomes– Virus removal

R. H. Christenson, S. D. Studenberg, S. Beck-Davis and F. A. Sedor:Digoxin-like immunoreactivity eliminated from serum by centrifugal ultrafiltration before fluorescence polarization immunoassay of digoxin.Clinical Chemistry 33, 606-608 (1987)

SpecificationsCentrisart® IConcentrator capacity Swing-bucket rotor

Fixed-angle rotor2.5 ml2.5 ml


93 mm14 mm0.79 cm2

< 5 µl100 µl

Materials of construction Centrifuge tubeFloater tubeCapMembrane

PolystyreneCellulose propionatePolyethyleneCTA, PES

Ordering InformationQty./Pkg. Prod. No.

5,000 MWCO CTA 12 13229-E10,000 MWCO CTA 12 13239-E20,000 MWCO CTA 12 13249-E100,000 MWCO PES 12 13269-E300,000 MWCO PES 12 13279-EStarter pack (3 units each of 5 k, 10 k, 20 k, 100 k) 12 13209-E

Retentate

Ultrafiltrate

Ultrafilter

Centrifugal force

Ultrafiltrate is collected in the floater insert tube, where it is readily accessible without removal of the tube.

Direction ofultrafiltration

* Centrisart is a registered trademark in the U.S. and the European Union


2 ml to 4 ml SamplesVivaspin® Turbo 4 is the newest member of the ultrafiltration family and allows the fastest sample concentration with the highest recoveries.

This device can handle up to 4 ml sample volumes in swing-bucket rotors and in fixed-angle rotors that accept 15 ml centrifuge tubes.

The Vivaspin® Turbo 4 optimized designand sleek internal profile ensure maximumprocess speeds all the way down to thelast few microliters, resulting in more than 100-fold concentration.

The UV joining technology ensures smooth joint transition between the membrane and the plasting housing – allowing removal of the entire sample concentrated in the unique, pipette-friendly dead-stop pocket.

122

17

SpecificationsVivaspin® Turbo 4Concentrator capacity Swing-bucket rotor


Dimensions Total lengthWidthActive membrane areaHold-up volume of membraneDead-stop volume swing-bucket rotorDead-stop volume fixed-angle rotor (25°)

122.5 mm17 mm3.2 cm2

< 10 µl40 µl

60 µl

Materials of construction Body

Filtrate vesselConcentrator capMembrane

Styrene butadiene copolymerePolypropylenePolypropylenePolyethersulfone

Ordering InformationVivaspin® Turbo 4 Polyethersulfone Qty./Pkg. Prod. No.3,000 MWCO 25 VS04T913,000 MWCO 100 VS04T925,000 MWCO 25 VS04T115,000 MWCO 100 VS04T1210,000 MWCO 25 VS04T0110,000 MWCO 100 VS04T0230,000 MWCO 25 VS04T2130,000 MWCO 100 VS04T2250,000 MWCO 25 VS04T3150,000 MWCO 100 VS04T32100,000 MWCO 25 VS04T41100,000 MWCO 100 VS04T42

Vivaspin® Turbo 4


2 ml to 6 ml SamplesVivaspin® 6 ml concentrators have been developed to offer increased volume flexibility and performance.

Vivaspin® 6 can process an impressive 6 ml in either swing-bucket or fixed-angle rotors accepting standard 15 ml conical bottom test tubes.

The Vivaspin® 6 features twin vertical membranes for unparalleled filtration speeds and 100 + plus concentrations. The remaining volume is easy to read off the printed scale on the side of the concentrator and the modified dead-stop pocket further simplifies direct pipette recovery of the final concentrate.

17

122 0.5

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4

6

5

10

15

20

0.2

0.5

0.75

1




122 mm17 mm2.5 cm2

< 10 µl30 µl


PolycarbonatePolycarbonatePolypropylenePolyethersulfone

Ordering InformationVivaspin® 6 Polyethersulfone Qty./Pkg. Prod. No.3,000 MWCO 25 VS06913,000 MWCO 100 VS06925,000 MWCO 25 VS06115,000 MWCO 100 VS061210,000 MWCO 25 VS060110,000 MWCO 100 VS060230,000 MWCO 25 VS062130,000 MWCO 100 VS062250,000 MWCO 25 VS063150,000 MWCO 100 VS0632100,000 MWCO 25 VS0641100,000 MWCO 100 VS0642300,000 MWCO 25 VS0651300,000 MWCO 100 VS06521,000,000 MWCO 25 VS06611,000,000 MWCO 100 VS06620.2 µm 25 VS06710.2 µm 100 VS0672Starter pack (5 of each 5 k, 10 k, 30 k, 50 k, 100 k)

25 VS06S1

Vivaspin® 6


4 to 15 ml SamplesVivaspin® 15R is designed for the volume segment of 2 to 15 ml and features a modified regenerated cellulose membrane; Hydrosart®. This membrane is ideal where extremely high recovery with very low adsorption is needed. Examples of these applications include desalting and concentration of Ig fractions.

Advantages– Ultimate recovery with low

adsorption (95-98%) – Exceptionally fast concentration time

(30 + in 15 min.)– Convenient application protocol

with easy handling– Easy scale-up to Vivaflow 200 with

Hydrosart® membrane for volumes up to 5 liters

– Very low hold-up volume (< 20 µl)

Vivaspin® 15R

5

10

15

30

116

SpecificationsVivaspin® 15RConcentrator capacity Swing-bucket rotor

Fixed-angle rotor15 ml12.5 ml


116 mm30 mm3.9 cm2

< 20 µl30 µl


PolycarbonatePolypropylenePolycarbonateHydrosart®

Ordering Information

Vivaspin® 15R Hydrosart® Qty./Pkg. Prod. No.2,000 MWCO 12 VS15RH91

2,000 MWCO 48 VS15RH925,000 MWCO 12 VS15RH115,000 MWCO 48 VS15RH1210,000 MWCO 12 VS15RH0110,000 MWCO 48 VS15RH0230,000 MWCO 12 VS15RH2130,000 MWCO 48 VS15RH22

Spin

Recover


4 to 15 ml SamplesVivaspin® Turbo 15 enables the fastest sample concentration with the highest recoveries. This device can handle a sample volume of up to 15 ml in swing-bucket rotors and of up to 11 ml in fixed-angle rotors that accept 50 ml centrifuge tubes.

The optimized design and sleek internal profile of the Vivaspin® Turbo 15 ensure maximum process speeds all the way down to the last few microliters, which results in more than 100-fold concentration.

The UV joining technology ensures smooth joint transition between the membrane and the plasting housing – allowing removal of the entire sample concentrated in the unique, pipette-friendly dead-stop pocket.

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Vivaspin® Turbo 15

SpecificationsVivaspin® Turbo 15Materials Body

Filtrate vessel Concentrator cap Membrane

Styrene butadiene copolymer Polypropylene Polypropylene Polyethersulfone (PES)

Dimensions Total length (concentrator insert) Total length (in tube with cap) Diameter (concentrator insert) Active membrane area Hold-up volume of membrane Dead-stop volume for swing-bucket rotor Dead-stop volume for fixed-angle rotor

77 mm 118 mm 27 mm 7.2 cm2 <10 µl 100 µl 60 µl

Concentrator capacity Swing-bucket rotor Fixed-angle rotor (25°)

15 ml 11 ml

Maximum speed 4,000 + g 4,000 + gSterilization ETO or 70% EtOHRemoval of endotoxins [Depyrogenization]

Flushing with 1N NaOH


Vivaspin® Turbo 15 Polyethersulfone Qty./Pkg. Prod. No.3,000 MWCO 12 VS15T913,000 MWCO 48 VS15T92

5,000 MWCO 12 VS15T115,000 MWCO 48 VS15T1210,000 MWCO 12 VS15T0110,000 MWCO 48 VS15T0230,000 MWCO 12 VS15T2130,000 MWCO 48 VS15T2250,000 MWCO 12 VS15T3150,000 MWCO 48 VS15T32100,000 MWCO 12 VS15T41100,000 MWCO 48 VS15T42


5 to 20 ml SamplesVivaspin® 20 ml centrifugal concentrators have been developed to offer increased volume flexibility and performance.

Vivaspin® 20 handles up to 20 ml in swing-bucket centrifuges and 14 ml in 25° fixed-angle rotors that accept 50 ml centrifuge tubes.

Featuring twin vertical membranes for unparalleled filtration speeds, the Vivaspin® 20 can achieve 100x plus concentrations.

The remaining volume is easy to read off the printed scale on the side of the concentrator and the modified dead-stop pocket further simplifies direct pipette recovery of the final concentrate.

More Process FlexibilityVivaspin® 20 is available with unique accessories and operating methods that are designed to provide more process flexibility and further time savings.

Gas Pressure FiltrationWhen an appropriate centrifuge is unavailable or for single sample processing, Vivaspin® 20 can be filled with up to 15 ml and then pressurized for bench-top concentration. For even faster processing, gas pressure can be combined with cen trifugal force. “Pressure-fugation” is particularly suitable for difficult or viscous samples, such as serum, or for use of a low process temperature, which reduces filtration speed, and generally when minimum process time is essential.

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Vivaspin® 20


Fixed-angle rotorWith pressure head

20 ml14 ml15 ml

Dimensions Total length

WidthActive membrane areaHold-up volume of membraneDead-stop volume

116 mm 125 mm with pressure head30 mm6.0 cm2

< 20 µl50 µl

Materials of construction BodyFiltrate vesselConcentrator capPressure headMembrane

PolycarbonatePolycarbonatePolypropyleneAcetal|AluminumPolyethersulfone


Vivaspin® 20 Polyethersulfone Qty./Pkg. Prod. No.3,000 MWCO 12 VS20913,000 MWCO 48 VS20925,000 MWCO 12 VS20115,000 MWCO 48 VS201210,000 MWCO 12 VS200110,000 MWCO 48 VS2002


Vivaspin® 20 Polyethersulfone Qty./Pkg. Prod. No.30,000 MWCO 12 VS202130,000 MWCO 48 VS202250,000 MWCO 12 VS203150,000 MWCO 48 VS2032100,000 MWCO 12 VS2041100,000 MWCO 48 VS2042300,000 MWCO 12 VS2051300,000 MWCO 48 VS20521,000,000 MWCO 12 VS20611,000,000 MWCO 48 VS20620.2 µm 12 VS20710.2 µm 48 VS2072Starter pack (2 each of 5 kDa, 10 kDa, 30 kDa, 50 kDa, 100 kDa, 0.2 µm)

12 VS20S1

Vivaspin® 20 Accessories Qty./Pkg. Prod. No.

Air pressure controller (APC) 1 VCA002

Charge valve for pressure head 1 VCA005Diafiltration cups 12 VSA005Female connector 1 VCA010Male connector 1 VCA0114 mm OD pneumatic tube (3 m) 1 VCA012Vivaspin® 20 pressure head 1 VCA200

Using the Vivaspin® 20 pressure cap

Pressurizeup to 5 bar withair or inert gas

Either

Then recover

Stand in rack

or

Spinup to 3,000 + g


Vivaclear centrifugal filters are disposable microfiltration devices for the fast and reliable clarification|filtration of biological samples in the range 100 µl to 500 µl. They can be used in fixed-angle rotors accepting 2.2 ml centrifuge tubes.

Product Features– High-flux polyethersulfone membrane– 0.8 µm pore size– Low hold-up volume (<5 µl)– Fast and reproducible performance

Applications– Clarification of samples before loading

in Vivapure® protein purification spin columns

– Removal of particles and precipitates– Filtration of plasma and serum– Filtration of cells or cell debris

Vivaclear Centrifugal Filters

SpecificationsVivaclear Centrifugal FiltersRotor 40–45° fixed-angle rotor, 500 µlPore size 0.8 µmDimensions Total length

Filtrate collection tube diameterActive membrane areaHold-up volume, membrane plus supportMaximum RCF

43 mm11 mm0.34 cm2

< 5 µl2,000 + g

Materials of construction BodyMembraneFiltrate collection tube

PolypropylenePolyethersulfonePolypropylene


Qty./Pkg. Prod. No.Vivaclear Mini 0.8 µm PES 100 VK01P042


10 ml to 70 ml SamplesVivacell 70 combines the ease of use of centrifugal devices with the flexibility and control provided by pressurized ultrafiltration cells. Vivacell 70 is inexpensive, quick and easy to assemble, requires no tubing con nections or stirring mechanisms and can be adapted to equipment availability or to specific user preferences.

For convenience, simply spin in a large capacity centrifuge (rotors accepting 250 ml bottles). For the highest speeds, parti cularly with difficult samples, pressurize the device with air or inert gas before centrifuging.

For more process control or for single samples, combine gas pressure with a gentle orbital shake, or you can even pressurize and then leave standing on a bench top or in a refrigerator for the highest simplicity with minimum equipment requirements.

The longitudinal membrane inhibits fouling, while the built-in dead stop will hinder further concentration when the residual volume drops below 150 µl.

Vivacell 70

SpecificationsVivacell 70Concentrator capacity Swing-bucket rotor

Fixed-angle rotorWith pressure headWith pressure-fuge head

70 ml50 ml70 ml50 ml



119 mm standard centrifugal185 mm with pressure head125 mm with pressure fuge head62 mm20 cm2

< 200 µl150 µl

Operating requirements Rotor typeMinimum rotor angleRotor cavity

Maximum speedMaximum pressure

Swing-bucket or fixed angle25°To fit 250 ml (62 mm) centrifuge bottles1,000 g5 bar (75 psi)

Materials of construction BodyFiltrate vesselConcentrator capPressure head|pressure fuge headMembrane

PolycarbonatePolycarbonateSantoprene

AcetalPolyethersulfone



Vivacell 70 Polyethersulfone – Concentrator Bodies With Polycarbonate Filtrate Bottles

Qty./Pkg. Prod. No.

5,000 MWCO 2 VS601110,000 MWCO 2 VS600130,000 MWCO 2 VS602150,000 MWCO 2 VS6031100,000 MWCO 2 VS60410.2 µm 2 VS6071

Vivacell 70 Polyethersulfone – Concentrator Body Only Qty./Pkg. Prod. No.5,000 MWCO 10 VS601210,000 MWCO 10 VS6002

30,000 MWCO 10 VS602250,000 MWCO 10 VS6032100,000 MWCO 10 VS60420.2 µm 10 VS6072


1

4

2

3

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RECOVERPRESSURISE

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4

2

3

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SHAKE

RECOVERPRESSURISE

CENTRIFUGE

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4

2

3

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RECOVERPRESSURISE

CENTRIFUGE

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4

2

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RECOVERPRESSURISE

CENTRIFUGE

1

4

2

3

FILL

SHAKE

RECOVER

PRESSURISE

CENTRIFUGE

Centrifuge – Process

convenience– Low shear, no

foaming– Less visual control

Pressure – Simplicity and

highest process control

– Ideal for refrige-rated use

– Slower concentrations

Pressure-Shake – Speed and process

control– Ideal for single

samples– If left unattended

can concentrate to dryness

Pressure-Fuge – Fastest processing – Ideal with low

MWCO or with difficult solutions

– Less visual control

Vivacell 70 Accessories Qty./Pkg. Prod. No.Air pressure controller (APC), complete with pressure gauge, regulator, over-pressure safety valve, female connector to Sartorius Stedim Biotech pressure products and 1 m extension line (4 mm pneumatic tubing) with male and female connectors and 1 m of 6 mm inlet tubing

1 VCA002

250 ml centrifuge bottle – standard caps 4 VSA003Modified caps for use in fixed-angle rotors with 250 ml centrifuge bottles

2 VCA004

Charge valve for pressure-fuge head 1 VCA005Replacement seals for pressure-fuge head (VCA701) 10 VCA007Female connector 1 VCA010Male connector 1 VCA0114 mm pneumatic tubing (3 m) 1 VCA012Vivacell 70 pressure head with reservoir and filtrate bottle (bench-top use)

1 VCA700

Vivacell 70 pressure-fuge head (for use in centrifuge) 2 VCA701


20 ml to 100 ml SamplesVivacell 100 is the latest member of the Vivacell family and bridges the volume range between the Vivacell 70 and the Vivacell 250.

The patented vertical membrane design ensures the highest performance and unmatched flexibility.

Vivacell 100 is a unique and innovative concentrator for volumes from 20 ml to 100 ml, and utilizes pressure, centrifuge or pressure-shake to rapidly concentrate even samples with very high-particle loading.

Vivacell 100 is designed for centrifugal concentration of samples up to 100 ml, which makes it the largest centrifugal unit available. At the same time, its new design allows for maximum centrifugal force of 4,000 + g to be used for even faster concentration.

Vivacell 100 Utilizes:– Pressure– Centrifuge– Pressure-shake

Like the smaller Vivacell 70 unit, Vivacell 100, when used as a centrifugal device, fits only into rotors that accept 250 ml bottles.

Vivacell 100 units can also be used for single or extremely sensitive samples in the pressurized mode only and left on a bench or placed on a swing-bucket laboratory shaker for faster concentration. It can also be kept in a pressurized mode in the refrigerator. Handling is facilitated by the use of quick connectors. In whichever mode Vivacell 100 is used, the vertical membrane design inhibits membrane fouling, while the built-in dead stop impedes concentration to dryness and loss of sample.

Vivacell 100

Filtrate container fits standard 250 ml rotors

Quick release connector

Pressure head Santoprene

seal

Twin vertical membranes

Concen-trator

Concen-trator cap Graduations

SpecificationsVivacell 100Concentrator capacity Swing-bucket rotor

With pressure head90 ml98 ml



123 mm centrifugal 197 mm with pressure head62 mm23.5 cm2

< 250 µl350 µl

Operating requirements Rotor typeRotor cavity

Maximum speedMaximum pressure

Swing-bucketTo fit 250 ml (62 mm) centrifuge bottles (maximum cavity depth 105 mm)2,000 g5 bar (75 psi)

Materials of construction BodyFiltrate vesselConcentrator capPressure headMembrane

PolycarbonatePolycarbonateSantopreneAcetalPolyethersulfone



Vivacell 100 Polyethersulfone with Polypropylene Concentrator Cap

Qty./Pkg. Prod. No.

5,000 MWCO 2 VC10115,000 MWCO 10 VC101210,000 MWCO 2 VC100110,000 MWCO 10 VC100230,000 MWCO 2 VC102130,000 MWCO 10 VC102250,000 MWCO 2 VC103150,000 MWCO 10 VC1032100,000 MWCO 2 VC1041100,000 MWCO 10 VC1042300,000 MWCO 2 VC1051300,000 MWCO 10 VC10521,000,000 MWCO 2 VC10611,000,000 MWCO 10 VC10620.2 µm 2 VC10710.2 µm 10 VC1072

Vivacell 100 Accessories Qty./Pkg. Prod. No.Air pressure controller (APC), complete with pressure gauge, regulator, over-pressure safety valve, female connector, 1 m extension line (4 mm pressure tubing) with male and female connectors and 1 m of 6 mm inlet tubing

1 VCA002

Female connector 1 VCA010Male connector 1 VCA0114 mm pressure tubing (3 m) 1 VCA012Santoprene replacement seals 10 VCA014Vivacell 100 pressure head with replacement seals (5)

1 VCA800

Centrifuge – Process convenience – Low shear, non-foaming – Less visual control

Pressure – Simplicity and the

highest process control– Ideal for refrigerated use– Slower concentrations

Pressure-Shake – Speed and process

control– Ideal for single samples


50 ml to 250 ml SamplesThe Vivacell 250 is a totally new concept for the concentration of relatively high-volume biological samples. This product offers numerous advantages when compared to stirred cells.

– One size handles a volume range from under 50 ml to 250 ml.

– Use free standing on a bench top or in a refrigerator for maximum simplicity, or use a laboratory shaker for fastest concentrations.

– The unique, conical dead stop built into the bottom of the membrane insert allows concentrations to under 1 ml.

– The gentle vortex action controls membrane polarization, while greatly reducing the shear effects that are typical of stirring mechanisms.

– Setup or membrane replacement takes just a few seconds. Quick-connect fittings and a simple screw closure further enhance ease of use.

This unequaled membrane module takes only seconds to replace. The concentrate can be easily monitored through the graduated inspection window.

Screw closure

Combined pressure indicator and safety valve

Quick-connect check valve

Membrane module takes seconds to replace

Unique conical base allows concentrations to under 1 mlConcentrate

inspection window

Vivacell 250

Specifications

Vivacell 250Concentrator capacityMax pressure

250 ml4 bar (60 psi)

Dimensions WidthHeight (incl. pressure indicator)Active membrane areaHold-up vol. memb. & supportDead-stop volume

116 mm235 mm40 cm2

< 200 µl600 µl

Materials of construction Screw closurePressure headQuick release connectorConcentrator body|sleeveFiltrate container

AcetalAcetalAcetalPolycarbonatePolycarbonate



Vivacell 250 Qty./Pkg. Prod. No.Vivacell 250 complete with pressure head, pressure indicator| over-pressure safety valve, quick-release connection to APC, 2 sample reservoirs, filtrate container and insert tool

1 VCA250

Vivacell 250 Polyethersulfone Inserts Qty./Pkg. Prod. No.5,000 MWCO 5 VC251110,000 MWCO 5 VC250130,000 MWCO 5 VC252150,000 MWCO 5 VC2531100,000 MWCO 5 VC25410.2 µm 5 VC2571Starter kit (1 each of 5 kDa, 10 kDa, 30 kDa, 50 kDa, 100 kDa) 5 VC25S1

Vivacell 250 Accessories Qty./Pkg. Prod. No.Air pressure controller (APC), complete with pressure gauge, regulator, over-pressure safety valve, female connector to Sartorius Stedim Biotech pressure products and 1 m extension line (4 mm pneumatic tubing) with male and female connector and 1 m of 6 mm inlet tubing

1 VCA002

Replacement pressure indicator|over-pressure safety valve 1 VCA008Vivacell 250 maintenance kit (includes one sample reservoir and filtrate container and O-ring seals for pressure head)

1 VCA009

Female connector 1 VCA010Male connector 1 VCA0114 mm OD pressure tubing (3 m) 1 VCA012Replacement pressure head and screw closure 1 VCA015


100 ml to 3 l Samples The unique, patented Vivaflow®* 50 system provides ease of use, performance, flexibility and economy that are unrivaled by any laboratory or pilot-scale filtration system on the market.

Unique Features– The thin-channel, flip-flow recirculation

path provides high crossflow velocities with minimum pump requirements

– No need for pressure holders– Crystal clear for simple checking

of remaining hold-up volume and membrane status

– Unique interlocking modules with series connectors for easy scale up

– Disposable

Unique Performance– A single 50 cm2 module will typically

reduce 500 ml to less than 15 ml in under 50 minutes

– Less than 10 ml minimum system recirculation for the highest concentrations

– Less than 500 µl non-recoverable hold-up volume

– Nearly total recoveries achievable with a single 10 ml rinse

Unique “flip-flow” thin channel flow path results in high turbulence and linear velocity for exceptional flux even at high concentrations

Multiple modules

Single module

Sample|diafiltration reservoir (VFA006)

Filtrate vessel

Vivaflow® 50

Specifications

Vivaflow® 50Dimensions Overall L + H + W

Channel W + HActive membrane areaHold-up volume (module)Minimum recirculation volumeNon-recoverable hold-up

107 mm + 84 mm + 25 mm15 mm + 0.3 mm50 cm2

1.5 ml< 10 ml< 0.5 ml

Operating conditions Pump flowMaximum pressureMaximum temperature

200 ml/min to 400 ml/min3 bar (45 psi)60°C

Materials of construction Main housingFlow channelMembrane supportSeals and O-ringsPressure indicatorFlow restrictorFittingsTubing

PolycarbonateTPX (PMP)TPX (PMP)SiliconePolypropylene, SS springPolypropyleneNylonPVC (medical grade)

Vivaflow® is a registered trademark in the U.S., Japan and the European Union.



Vivaflow® 50* Qty./Pkg. Prod. No.3,000 MWCO PES 2 VF05P95,000 MWCO PES 2 VF05P110,000 MWCO PES 2 VF05P030,000 MWCO PES 2 VF05P250,000 MWCO PES 2 VF05P3100,000 MWCO PES 2 VF05P41,000,000 MWCO PES 2 VF05P60.2 µm PES 2 VF05P7100,000 MWCO RC 2 VF05C4

* Vivaflow® 50 modules include filtrate tube, size 16 peristaltic tubing, flow restrictors and fittings.

Vivaflow® 50 Complete System ComprisesPump (240 V), Easy Load pump head (size 16), tubing, 500 ml sample|diafiltration reservoir, module stand, pressure indicator, T-connectors, series interconnectors

1 VFS502

Pump (115 V), Easy Load pump head (size 16), tubing, 500 ml sample|diafiltration reservoir, module stand, pressure indicator, T-connectors, series interconnectors

1 VFS504

PVC Tubing and FittingsSize 16 PVC pump tubing (3 m, 3.2 + 1.6 mm) VFA004

Flow restrictor set (2 + 0.4 mm, 0.6 mm, 0.8 mm) VFA009

T-connectors for running 2 stacks (2 units) VFA030Series interconnectors (6 units) VFA031Female luer fittings (10 units) VFA032

VF50 tubing kit (2 + 1 m size 16 PVC tubing with inlet fittings, 2 + 50 cm size 16 PVC tubing with 0.6 mm flow restrictors, 1 + series interconnector)

VFA034

Flow restrictor 0.6 mm (6 units) VFA035

AccessoriesMasterflex economy drive variable speed peristaltic pump (240 V) VFP001Masterflex economy drive variable speed peristaltic pump (115 V) VFP002500 ml sample and|or diafiltration reservoir VFA006Masterflex Easy Load pump head – size 16 VFA012Vivaflow® 50 stand VFA016Pressure indicator (1 to 3 bar [15 psi to 45 psi]) VFA020


0.1 ml to 1 l SamplesConcentrate 100 ml to under 20 ml in just a few minutes or concentrate one liter 50 times in less than 60 minutes. Alternatively, speed up your process by using two Vivaflow® 50R units in parallel and concentrate 1 liters in under 30 min.

Vivaflow® 50R is a plug-and-play laboratory crossflow cassette for concen-trating up to 1 L aqueous samples. The active membrane area per device is 50 cm2. One unit comes with all the necessary accessories for running the device with a laboratory pump and a size 16 pump head. For speeding up concentration, two cassettes can be run simultaneously.

– Fast and easy protein sample concentration

– Reusable– Concentrates volumes from 0.1 l to 1 l– Optimal for concentration of culture

supernatants and viruses– The most compact crossflow cassette

with a premium Hydrosart® membrane

Vivaflow® 50R

Specifications

Vivaflow® 50RDimensions Overall L + H + W

Channel W + H Active membrane area Hold-up volume (module) Min. recirculation volumeNon-recoverable hold-up

100 mm + 100 mm + 24 mm7.5 mm + 0.4 mm50 cm2

1.7 ml10 ml< 0.5 ml



Materials of construction Main housingFlow channelMembrane supportSeals and O-ringsPressure indicatorFlow restrictorFittingsTubing

AcrylicAcrylicPolypropyleneSiliconePolypropylene, SS springPolypropyleneNylonPVC (medical grade)

Vivaflow® 50R – Two modules

Return

PumpFiltrate

Sample| Feed

Vivaflow® 50R – Single module

Return

PumpFiltrate

Sample| Feed



Vivaflow® 50R* Qty./Pkg. Prod. No.5,000 MWCO Hydrosart® 1 VF05H110,000 MWCO Hydrosart® 1 VF05H030,000 MWCO Hydrosart® 1 VF05H2100,000 MWCO Hydrosart® 1 VF05H4

* Vivaflow® 50R modules include pressure indicator, flow restrictor and size 16 pvc peristaltic tubing and fittings.

Vivaflow® 50R/200 Complete SystemPump (240 V), Easy Load pump head (size 16), tubing, 500 ml sample | diafiltration reservoir

1 VFS202

Pump (115 V), Easy Load pump head (size 16), tubing, 500 ml sample | diafiltration reservoir

1 VFS204

Tubing and FittingsSize 16 pvc pump tubing and Luer fittings (3 m, 3.2 × 1.6 mm)

1 VFA004

T-connectors for running 2 units 2 VFA030Flow restrictor set (2 × 0.4 mm, 0.6 mm, 0.8 mm) 6 VFA009Female luer fittings – size 16 10 VFA032Flow restrictors 0.6 mm 6 VFA035Female luer fittings – size 15 10 VFA036

AccessoriesMasterflex economy drive variable speed peristaltic pump (240 V)

1 VFP001

Masterflex economy drive variable speed peristaltic pump (115 V)

1 VFP002

500 ml sample and | or diafiltration reservoir 1 VFA006Masterflex Easy Load pump head – size 16 1 VFA012


Vivaflow 200 setup for diafiltration

0.5 ml to 5 l SamplesConcentrate 250 ml to under 20 ml in just a few minutes or concentrate one lirwe 50 times in less than 30 minutes. Alternatively, use two Vivaflow® 200 units in parallel and concentrate 5 liters in under 75 minutes.

Nearly total sample recoveries can be expected with most solutions.

The standard economy package comes complete with tubing, pressure indicator, flow restrictor and high-pressure pump tubing. All you need is a peristaltic pump capable of handling 6.4 mm OD (size 16) tubing. Should your pump head require larger tubing, just use the interconnecter provided to attach your own peristaltic tubing to this standard product.

Two modules in parallel will concentrate 5 liters in under 75 minutes

Feed line

Feed reser-voir

Pump head

Sealed diafil-tra tion reservoir

Pressure indicator

Flow restrictor

Waste

Vivaflow® 200

Specifications

Vivaflow 200Dimensions Overall L + H + W

Channel W + HActive membrane areaHold-up volume (module)Min. recirculation volumeNon-recoverable hold-up

126 mm + 138 mm + 38 mm10 mm + 0.4 mm200 cm2

5.3 ml< 20 ml< 2 ml

Materials of construction Main housingFlow channelMembrane supportSeals and O-ringsPressure indicator

Flow restrictorFittingsTubing

AcrylicAcrylicPolypropyleneSiliconePolypropylene, SS springPolypropyleneNylonPVC (medical grade)





Vivaflow® 200* Qty./Pkg. Prod. No.3,000 MWCO PES 1 VF20P95,000 MWCO PES 1 VF20P110,000 MWCO PES 1 VF20P030,000 MWCO PES 1 VF20P250,000 MWCO PES 1 VF20P3100,000 MWCO PES 1 VF20P40.2 µm PES 1 VF20P72,000 MWCO Hydrosart® 1 VF20H95,000 MWCO Hydrosart® 1 VF20H1

10,000 MWCO Hydrosart® 1 VF20H030,000 MWCO Hydrosart® 1 VF20H2100,000 MWCO Hydrosart® 1 VF20H4

* Vivaflow® 200 modules include pressure indicator, flow restrictor and size 16 PVC peristaltic tubing and fittings.

Vivaflow® 200 Complete System ComprisesPump (240 V), Easy Load pump head (size 16), tubing, 500 ml sample|diafiltration reservoir

1 VFS202

Pump (115 V), Easy Load pump head (size 16), tubing, 500 ml sample|diafiltration reservoir

1 VFS204

AccessoriesMasterflex economy drive variable speed peristaltic pump (240 V) VFP001Masterflex economy drive variable speed peristaltic pump (115 V) VFP002500 ml sample and|or diafiltration reservoir VFA006Masterflex Easy Load pump head – size 16 VFA012Masterflex Easy Load pump head – size 15 VFA013

Tubing and FittingsSize 15 PVC pump tubing and Luer fittings (3 m, 4.8 mm + 2.6 mm) VFA003

Size 16 PVC pump tubing and Luer fittings (3 m, 3.2 mm + 1.6 mm) VFA004

Y-connetor (size 15 to 2 + size 16) VFA005

Flow restrictor set (2 + 0.4 mm, 0.6 mm, 0.8 mm) VFA009

Female luer fittings, size 16 (10 units) VFA032Flow restrictors 0.6 mm (6 units) VFA035Female luer fittings, size 15 (10 units) VFA036


3 ml to 20 ml SamplesWith no need for additional equipment, pressure or vacuum, solvent absorption is the most economic and user-friendly concentration technique available to the clinician and research scientist.

Just fill the unit with the solution to be concentrated, wait for the desired con-centration level to be achieved and then pipette the concentrated sample from the bottom of the reservoir.

Vivapore®* is ideal for general-purpose laboratory concentration and purification prior to further analysis. It is particularly suited for labile solutions that can denature with alternative shear- or pressure- inducing methods or that require processing in a cold room environment.

Vivapore® concentrators extend the solvent absorption technique to a totally new level of performance, application potential and ease of use.

Vivapore® 5

Vivapore® 10|20

Vivapore® Solvent Absorption Concentrators

SpecificationsVivapore® 5 Vivapore® 10|20

Membrane material PES PESMembrane MWCO 7,500 7,500Membrane surface area 20 cm2 28 cm2

Reservoir material SAN SANVolume range 1 ml to 5 ml 2 ml to 10 ml | 20 ml*Minimum concentrate volume 50 µl 50 µlVivapore® overall dimensionsWidth (mm)Height (mm)

4282

46100



Vivapore® 5* Qty./Pkg. Prod. No.7,500 MWCO PES 4 VP05037,500 MWCO PES 30 VP0501

* includes stand and recovery pipettes

Requires Stand7,500 MWCO PES 100 VP0502

Vivapore® 10|20*7,500 MWCO PES 4 VP20037,500 MWCO PES 30 VP2001

* includes stand and recovery pipettes

Requires Stand7,500 MWCO PES 100 VP2002

AccessoriesDisposable stands for 4 units 6 VPA002Plastic recovery pipettes (Vivapore® 10|20) 100 VPA00510 ml expansion reservoir (Vivapore® 10|20) 10 VPA006Plastic recovery pipettes (Vivapore® 5) 100 VPA007


Polyethersulfone (PES)This is a general purpose membrane that provides excellent performance with most solutions when retentate recovery is of primary importance. Polyethersulfone membranes exhibit no hydrophobic or hydrophilic interactions and are usually preferred for their low fouling characteristics, exceptional flux and broad pH range.

Cellulose Triacetate (CTA)High hydrophilicity and exceptionally low non- specific binding characterize this membrane. Cast without any membrane

support that could trap or bind passing micro solutes, these membranes are to be ideal for sample cleaning and protein removal and when high recovery of the filtrate solution is of primary importance.

Hydrosart®

These membranes are also highly hydrophilic and are often preferred for their high protein recovery when processing very dilute solutions. Resistance to autoclaving, ease of cleaning and extended chemical resistance also characterize this type of membrane.

Ultrafiltration Membrane Filters PES 146, CTA 145 and Hydrosart® 144

SpecificationsTypical Performance for Polyethersulfone, Type 146Thickness 120 µmpH range 1–14Water flux MWCO 10,000 0.2 ml/min/cm2

Protein retention Cytochrome C 95%

Specifications for Cellulose Triacetate, Type 145Thickness 120 µmpH range 4–8Water flux MWCO 10,000 0.11 ml/min/cm2


Specifications for Hydrosart®, Type 144Thickness 180 µmpH range 1–13

Water flux MWCO 10,000 0.08 ml/min/cm2



Polyethersulfone Membrane Filters, Type 146

Diameter in mm MWCO Qty./Pkg. Prod. No.47 1,000 daltons 10 14609--47------D63 1,000 daltons 10 14609--63------D76 1,000 daltons 10 14609--76------D25 5,000 daltons 10 14629--25------D47 5,000 daltons 10 14429--47------D63 5,000 daltons 10 14629--63------D76 5,000 daltons 10 14629--76------D25 10,000 daltons 10 14639--25------D


Polyethersulfone Membrane Filters, Type 146

Diameter in mm MWCO Qty./Pkg. Prod. No.63 10,000 daltons 10 14639--63------D76 10,000 daltons 10 14639--76------D150 10,000 daltons 10 14639-150------D

25 30,000 daltons 10 14659--25------D63 30,000 daltons 10 14659--63------D76 30,000 daltons 10 14659--76------D25 50,000 daltons 10 14650--25------D47 50,000 daltons 10 14650--47------D76 50,000 daltons 10 14650--76------D25 300,000 daltons 10 14679--25------D47 300,000 daltons 10 14679--47------D76 300,000 daltons 10 14679--76------D

Cellulose Triacetate Membrane Filters, Type 145

Diameter in mm MWCO Qty./Pkg. Prod. No.25 5,000 daltons 10 14529--25------D47 5,000 daltons 10 14529--47------D25 10,000 daltons 10 14539--25------D47 10,000 daltons 10 14539--47------D50 10,000 daltons 10 14539--50------D25 20,000 daltons 10 14549--25------D43 20,000 daltons 10 14549--43------D47 20,000 daltons 10 14549--47------D47 20,000 daltons 100 14549--47------N63 20,000 daltons 10 14549--63------D25 30,000 daltons 10 14459--25------D76 30,000 daltons 10 14459--76------D

Hydrosart® Membrane Filters, Type 144

Diameter in mm MWCO Qty./Pkg. Prod. No.25 5,000 daltons 10 14429--25------D44 5,000 daltons 10 14429--44------D63 5,000 daltons 10 14429--63------D76 5,000 daltons 10 14429--76------D25 10,000 daltons 10 14439--25------D47 10,000 daltons 10 14439--47------D63 10,000 daltons 10 14439--63------D

76 10,000 daltons 10 14439--76------D25 30,000 daltons 100 14459--25------D47 30,000 daltons 10 14459--47------D

63 30,000 daltons 10 14459--63------D76 30,000 daltons 10 14459--76------D

36 Ultrafiltration DNA Concentration

37UltrafiltrationDNA Concentration

Reproducible DNA and Protein Sample Desalting and ConcentrationVivacon® 500 centrifugal concentrators offer the optimal solution for DNA and protein concentration and buffer exchange applications. For optimal performance with highly dilute samples, e.g. forensic samples, Vivacon® 500 incorporates the patented regenerated cellulose membrane Hydrosart®.

High recoveries and excellent reproducibilities are combined with convenience offered by the molecular weight cutoff printed on the individual Vivacon® 500 units.

As Vivacon® 500 can be respun after sample processing, this ensures complete concentrate recovery, which is especially important when working with low-sample concentrations.

Vivacon® 500-PCR GradeTo use DNA amplification technologies, any traces of DNA originating from the equipment need to be eliminated.

Vivacon® 500-PCR Grade units are treated with ethylene oxide (ETO) in a validated process to deactivate all traces of DNA that might interfere with subsequent amplification procedures.

References: K. Shaw et al., Int. J. Legal Med. (2008) 122: 29–33

Vivacon® 500 For DNA Sample Desalting and Concentration

SpecificationsVivacon® 500Concentrator capacity Fixed-angle rotor 0.5 mlDimensions Total length (concentration)

Total length (backspin)WidthActive membrane areaHold-up volume of membrane and support Dead-stop volume (40° rotor)

45 mm47.5 mm12.4 mm0.32 cm2

< 5 µl5 µl

Materials of construction BodyFiltrate vesselMembrane

PolycarbonatePolypropyleneHydrosart®

Conversion Table for Hydrosart® MWCO to Nucleotide Cutoff

Membrane MWCO Double-Stranded Nucleotide Cutoff (bp)

Hydrosart® 2 kDa > 10Hydrosart® 10 kDa > 30Hydrosart® 30 kDa > 50Hydrosart® 50 kDa > 300Hydrosart® 100 kDa > 600Cellulose Acetate 125 kDa > 650



Vivacon® 500 Qty./Pkg. Prod. No.2,000 MWCO 25 VN01H912,000 MWCO 100 VN01H9210,000 MWCO 25 VN01H0110,000 MWCO 100 VN01H0230,000 MWCO 25 VN01H2130,000 MWCO 100 VN01H2250,000 MWCO 25 VN01H3150,000 MWCO 100 VN01H32100,000 MWCO 25 VN01H41100,000 MWCO 100 VN01H42125,000 MWCO 25 VN01H81125,000 MWCO 100 VN01H82125,000 MWCO 500 VN01H83

Vivacon® 500 Qty./Pkg. Prod. No.Sample kit L (4 units each of 2 kDa, 10 kDa, 30 kDa)

12 VN01HL12

Sample kit H (4 units each of 30 kDa, 50 kDa, 100 kDa)

12 VN01HH12

Vivacon® 500-PCR Grade Sample Pack

Qty./Pkg. Prod. No.

30,000 MWCO 4 VN01H2SETO50,000 MWCO 4 VN01H3SETO100,000 MWCO 4 VN01H4SETO


Vivacon® 500-PCR Grade Qty./Pkg. Prod. No.30,000 MWCO 25 VN01H21ETO30,000 MWCO 100 VN01H22ETO30,000 MWCO 500 VN01H23ETO50,000 MWCO 25 VN01H31ETO50,000 MWCO 100 VN01H32ETO50,000 MWCO 500 VN01H33ETO100,000 MWCO 25 VN01H41ETO100,000 MWCO 100 VN01H42ETO100,000 MWCO 500 VN01H43ETO125,000 MWCO 25 VN01H81ETO125,000 MWCO 100 VN01H82ETO125,000 MWCO 500 VN01H83ETO

Accessories Qty./Pkg. Prod. No.Tubes 100 VNCT01


Reproducible DNA Sample Desalting and ConcentrationVivacon® 2 centrifugal concentrators offer the optimal solution for DNA and protein concentration and buffer exchange applications. For optimal performance with highly dilute samples, e.g. forensic samples, Vivacon® 2 incorporates the patented regenerated cellulose membrane Hydrosart®.

High recoveries and excellent reproducibilities are combined with the convenience provided by the volume graduation and molecular weight cutoff printed on the individual Vivacon® 2 units.

As Vivacon® 2 can be respun after sample processing, this ensures complete concentrate recovery, which is especially important when working with low-sample concentrations.

Vivacon® 2-PCR GradeVivacon® 2-PCR Grade units are treated with ethylene oxide (ETO) in a validated process to deactivate all traces of DNA that might interfere with subsequent amplification procedures.

SpecificationsConcentrator capacity Fixed-angle rotor 2 ml

Dimensions Total length (concentration)Total length (backspin)WidthActive membrane areaHold-up volume membrane and supportDead-stop volume (25° rotor)

125 mm115 mm16 mm0.95 cm2

10 µl55 µl

Materials of construction BodyFiltrate vesselBackspin vialConcentrator capMembrane

PolycarbonatePolypropylenePolypropylenePolypropyleneHydrosart®

Conversion Table for Hydrosart® MWCO to Nucleotide Cutoff

Membrane MWCO Double-Stranded Nucleotide Cutoff (bp)

Hydrosart® 2 kDa > 10Hydrosart® 10 kDa > 30Hydrosart® 30 kDa > 50Hydrosart® 50 kDa > 300Hydrosart® 100 kDa > 600Cellulose Acetate 125 kDa > 650

Vivacon® 2 For DNA Sample Desalting and Concentration



Vivacon® 2 Qty./Pkg. Prod. No.2,000 MWCO 25 VN02H912,000 MWCO 100 VN02H922,000 MWCO 500 VN02H9310,000 MWCO 25 VN02H0110,000 MWCO 100 VN02H0210,000 MWCO 500 VN02H0330,000 MWCO 25 VN02H2130,000 MWCO 100 VN02H2230,000 MWCO 500 VN02H2350,000 MWCO 25 VN02H3150,000 MWCO 100 VN02H3250,000 MWCO 500 VN02H33100,000 MWCO 25 VN02H41100,000 MWCO 100 VN02H42100,000 MWCO 500 VN02H43125,000 MWCO 25 VN02H81125,000 MWCO 100 VN02H82125,000 MWCO 500 VN02H83

Vivacon® 2-PCR Grade Qty./Pkg. Prod. No.30,000 MWCO 25 VN02H21ETO30,000 MWCO 100 VN02H22ETO30,000 MWCO 500 VN02H23ETO50,000 MWCO 25 VN02H31ETO50,000 MWCO 100 VN02H32ETO50,000 MWCO 500 VN02H33ETO100,000 MWCO 25 VN02H41ETO100,000 MWCO 100 VN02H42ETO100,000 MWCO 500 VN02H43ETO125,000 MWCO 25 VN02H81ETO125,000 MWCO 100 VN02H82ETO125,000 MWCO 500 VN02H83ETO

Vivacon® 2-PCR Grade Sample Pack Qty./Pkg. Prod. No.30,000 MWCO 4 VN02H2SETO50,000 MWCO 4 VN02H3SETO100,000 MWCO 4 VN02H4SETO

42 Ultrafiltration Endotoxin Concentration

43UltrafiltrationEndotoxin Concentration

Vivaspin® Endotest is a single-use ultrafiltration device for endotoxin concentration and removal of interfering substances from liquid samples for LAL testing. The Vivaspin® Endotest is certified as endotoxin-free and is available with a cellulose triacetate membrane that has a molecular weight cutoff (MWCO) of 20,000.

Due to its centrifugal design, Vivaspin® Endotest enables parallel testing to minimize hands-on time.

Vivaspin® Endotest can effectively be used in either swing-bucket or fixed-angle rotors that accept 50 ml conical bottom centrifuge tubes.

SpecificationsConcentrator capacity Swing-bucket rotor

Fixed-angle rotor15.0 ml 12.5 ml

Dimensions Total lengthDiameterActive membrane areaDead-stop volumeNon-recoverable (hold-up) volume

116 mm30 mm3.9 cm²30 µl< 10 µl


PolycarbonatePolycarbonatePolypropyleneCellulose triacetate


Vivaspin® Endotest Qty./Pkg. Prod. No.20,000 12 VS15RXETO

Vivaspin® Endotest

44 Ultrafiltration Protein Purification

45UltrafiltrationProtein Purification

Fast and Easy-to-Use Spin ColumnsVivapure® Ion Exchange (IEX) spin columns are centrifugal devices that incorporate Sartobind® membrane adsorber technology as their chromatography matrix. Vivapure® IEX spin columns make protein purification as easy as filtration. The devices are ready to use and eliminate the risk of running dry. For many protein purification applications, they replace time-consuming and tedious column chromatography.

The rapid Vivapure® IEX 1-2-3 bind-wash-elute protocol especially lends itself to screening applications, where many different samples are processed in parallel.

The Sartobind® Membrane Adsorber MatrixSartobind® IEX membrane adsorbers are based on stabilized regenerated cellulose and exhibit a microporous structure with a pore size of > 3 µm, which is orders of magnitude larger than conventional chromatographic gel materials. This allows molecules to be transported to the ligands immobilized on the membrane adsorber by convective flow, resulting in exceptionally high flow rates.

By contrast, gel chromatography is slowed down due to diffusion limitations, as the molecules need to enter the small bead pores in order to be bound by the ligands. The porous membrane adsorber enables fast, reproducible and scalable protein purification.

Fast and Simple-to-Use Spin Columns– Devices are ready to use– They make protein purification as simple

as filtration

Reproducible Results– No column packing necessary as the

devices are ready to use– Membrane adsorber spin columns cannot

crack or run dry

Centrifugal Devices– Offer the possibility of working in parallel

Low Bed Volume– Small membrane adsorber bed volumes

allow working with lower buffer amounts, resulting in concentrated elution fractions

Scalable Product Range– Process-scale modules are available

with the same Sartobind® IEX membrane adsorber matrix

Chromatography gel beads (right) are shown on top of a membrane adsorber in this SEM. The membrane adsorber pores are more than 50 times larger than bead pores.

Spin Spin

Original sample

Fast and easy protein purification with Vivapure® spin columns

Protein is bound to membrane

Wash Elute Pure protein

Vivapure® Ion Exchange Protein Purification Products


Specifications

Available Formats

Vivapure® IEX Products ApplicationVivapure® Mini spin columns – Sample fractionation

– Purification condition scouting – Small-scale purification

Vivapure® Maxi spin columns – Large-scale sample fractionation – One-step protein purification|concentration – Polishing of His-tagged protein

Membrane Availability

Functional Groups Ion Exchanger TypeSulfonic acid (S) Strong acidic cation exchanger: R-CH2-SO3

-Na+

Quaternary ammonium (Q) Strong basic anion exchanger: R-CH2-N+-(CH3)3Cl-

Diethylamine (D) Weak basic anion exchanger: R-CH2-NH+-(CH2H5)2

Performance Characteristics

Vivapure® Spin Columns

Protein Binding Capacity* (mg)

Max. Volume per Centrifuge Run Using a Swing-Out Rotor (ml)

Max. Volume per Centrifuge Using a Fixed-Angle Rotor Run (ml)

Vivapure® Mini H 4 0.4

Vivapure® Maxi H 60 to 80 19 10.5

* Actual yields depend on specific protein sample and selected pH and salt conditions. Yields established using 1 mg/ml BSA in 25 mM Tris/HCL pH 8.0 with Vivapure® Q & D spin columns and 1 mg/ml cytochrome c in 25 mM sodium acetate buffer pH 5.5 with Vivapure® S spin columns.


Description Spin Columns

Centrifuge Tubes

Prod. No.

Vivapure® Mini Ion Exchange Spin Columns (up to 0.5 ml)Vivapure® Mini S&Q H starter kit 16 32 VS-IX01SQ16

Vivapure® D Mini H 24 48 VS-IX01DH24

Vivapure® Q Mini H 24 48 VS-IX01QH24

Vivapure® S Mini H 24 48 VS-IX01SH24

Vivapure® Maxi Ion Exchange Spin Columns (up to 20 ml)Vivapure® D Maxi H 8 16 VS-IX20DH08

Vivapure® Q Maxi H 8 16 VS-IX20QH08

Vivapure® S Maxi H 8 16 VS-IX20SH08

Vivapure® Mini-400 | 500 µlBinding capacities: 1 mg to 4 mg

Vivapure® Maxi-19 | 20 mlBinding capacities: 15 mg to 80 mg


Rapid Purification with High YieldsVivapure® Miniprep and Maxiprep kits are spin-column-based kits for fast and effective purification of His-tagged proteins and antibodies.

Spin columns have the advantage of speed over gravity drip columns and batch protocols.

With the patented FlowGo regulator, the sample residence time is extended to assure adequate sample binding to the resin. As a result, Vivapure® Miniprep and Maxiprep spin column kits combine the merits of spin columns and gravity drip columns. This results in rapid purification with up to 95% protein recovery and purity.

All spin columns can be conveniently used in a centrifuge. For processing large sample volumes, e.g. from diluted cell culture supernatants, Vivapure® Maxiprep spin columns can additionally be run with a peristaltic pump collar (VS-PPCSC).

Vivapure® Miniprep and Maxiprep columns come in a variety of different configurations for your convenience. They can be purchased as completely ready-to-use kits with buffers and ultrafiltration devices but just as well as stand-alone spin columns in small or large packages for frequent users.

Place the plug in the spin column with the recessed end uppermost.

Loading the plug into the spin column

Top of plugReuse

Push the plug fully into the tapered end of the spin column using the plug insertion tool.

It is now ready for pre-equilibration with binding buffer followed by centrifugation.

After use, the plug is removed using the plug insertion tool.

Removing the plug from the spin column

Load Lock Spin

Vivapure® Maxiprep

Vivapure® Miniprep

Load LockSpin

Reuse

Top of plug

Loading the plug into the spin column

Place the plug in the spin column with the recessed end uppermost.

Push the plug fully into the tapered end of the spin column using the plug insertion tool.

It is now ready for pre-equilibration with binding buffer followed by centrifugation.

Vivapure® Miniprep and Maxiprep Purification Kits For Fast Antibody and His-Tagged Protein Purification



Product Name Qty./Pkg. Prod. No. Vivapure® A Starter Pack* 2 Miniprep A units VS-ARSTPKA2Vivapure® Miniprep A Kit* 16 Miniprep A units VS-ARAMINIKVivapure® Miniprep A Bulk Pack 48 Miniprep A units VS-ARAMINIBVivapure® Miniprep A Kit* 4 Maxiprep A units VS-ARAMAXIKVivapure® Maxiprep A Bulk Pack 12 Maxiprep A units VS-ARAMAXIBVivapure® A Buffer Pack VS-ARABUFPKVivapure® G Starter Pack* 2 Miniprep G units VS-ARSTPKG2Vivapure® Miniprep G Kit* 16 Miniprep G units VS-ARGMINIKVivapure® Miniprep G Bulk Pack 48 Miniprep G units VS-ARGMINIBVivapure® Maxiprep G Kit* 4 Maxiprep G units VS-ARGMAXIKVivapure® Maxiprep G Bulk Pack 12 Maxiprep G units VS-ARGMAXIBVivapure® G Buffer Pack VS-ARGBUFPKSealing cap and peristaltic pump collar 1 VS-PPCSC

* including ultrafiltration concentrators and buffers

Specifications

Protein A and G for Antibody Purification

Protein A and G Miniprep CentrifugeSample size 0.65 mlTypical binding capacity 1 mg IgG/columnNumber of reuses 3

Protein A & G Maxiprep Centrifuge1

Sample size 20 mlTypical binding capacity 20 mg IgG/columnNumber of reuses 51 Use the peristaltic pump accessory (VS-PPCSC) for larger volumes


Specifications

Protein MC Miniprep Kits CentrifugeSample size 0.65 mlTypical binding capacity 1 mg

His-tagged proteinNumber of reuses 2

Protein MC Maxiprep Kits

Centrifuge1

Sample size 20 mlTypical binding capacity 10 mg

His-tagged proteinNumber of reuses 21 Use the peristaltic pump accessory (VS-PPCSC) for larger volumes


Product Name Qty./Pkg. Prod. No. Vivapure® Metal Chelate (MC) Starter Pack* 4 VS-MCST04Vivapure® Miniprep MC Kit* 24 VS-MCMINI24Vivapure® Miniprep MC Bulk Pack 72 VS-MCMINIB

Vivapure® Maxiprep MC Kit* 8 VS-MCMAXIKVivapure® Maxiprep MC Bulk Pack 24 VS-MCMAXIBVivapure® Metal Chelate Buffer Pack VS-MCBUFPK

* including ultrafiltration concentrators and buffers

50 Ultrafiltration Virus Purification and Concentration

51UltrafiltrationVirus Purification and Concentration

Vivapure® Virus Purification and Concentration Kits

Recombinant virus vectors are the preferred method for a wide range of gene delivery applications. Especially adenovirus type 5 and VSV-G pseudotyped lentivirus are two frequently utilized viral vectors for in vitro and in vivo applications.

Recombinant Adenovirus Vectors Recombinant adenovirus vectors are versatile tools in research and therapeutic applications for gene transfer and protein expression in cell lines that have low transfection efficiency with liposomes. After entering cells, the virus remains epichromosomal – i.e., does not integrate into the host chromosome, leaving the host genome unaffected. The delivery of RNAi into cells is becoming a major application for adenovirus vectors.

Lentivirus Vectors Lentivirus vectors are frequently used in gene transfer studies, due to their ability of gene transfer and integration into dividing and non-dividing cells. The pseudotyped envelope with vesicular stomatitis virus envelope G (VSV-G) protein broadens their target cell range. Lentiviral vectors have been shown to deliver genes into cell types (e.g. neurons, lymphocytes and macrophages) which other retrovirus vectors could not be used for. The lentivirus vector is increasingly used to integrate siRNA efficiently in a wide variety of cell lines and primary cells, both in vitro and in vivo.

Rapid Virus Purification by Membrane ChromatographyThe Sartobind® ion exchange membrane adsorber technology used in AdenoPACK and LentiSELECT is unique in its capability to efficiently and rapidly capture and recover large virus particles. Compared with chromatography media, membrane adsorbers provide large 3,000 nm pores, allowing unrestricted access and recovery of virus from the charged adsorber surface. Convective flow through the syringe filter devices provides high-speed separations not possible with traditional chromatography, cesium chloride density gradients and ultracentrifugation methods. Sartorius membrane adsorbers with porous matrices, high capacities, low differential pressures, high flow rates and low unspecific adsorption show excellent performance in small-scale virus purification. In addition, these syringe filter devices are scalable and comply with cGMP requirements for large-volume, high-performance separation, reducing final process time ten-fold.


AdenoPACK 20|100|500The AdenoPACK adenovirus purification and concentration kits offer researchers who need to recover up to 3 + 1013 purified recombinant adenovirus particles for in vitro transfection a fast, safe and easy-to-use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adeno virus type 5 from HEK293 cell cultures – all within just two hours. These straightforward kits replace time- consuming and labor-intensive 48-hour CsCl density gradients.

AdenoPACK kits are offered as AdenoPACK 20, AdenoPACK 100 and AdenoPACK 500 for the purification and concentration of adenovirus type 5 from 20 ml to 500 ml cell cultures, resulting in 1 + 1011 to 3 + 1013 purified viral particles. For each sample volume, the most convenient handling method is provided for ultimate convenience.

To this end, preparations using AdenoPACK 20 are supplied in a spin column format for centrifuges. AdenoPACK 100 is a manually operated kit in a syringe filter format* and AdenoPACK 500 is a pump-driven kit.

* Vivapure® AdenoPACK 100 can be alternatively be operated with a laboratory pump or an infusion pump, for which protocols are provided on our web page at www.sartorius-stedim.com. Additionally, the tubes and adaptors needed for these operating modes can be ordered.

AdenoPACK AdvantagesFast and Easy Virus Purification– Purification completed in

just 2 hours– Convenient, over 10 + faster alternative

to CsCl density gradient

Quantitative Yields– In contrast to CsCl density gradient, the

complete cell culture is used for virus purification and not only the viral pellet

Flexible Product Range– Applicable from initial construct

screening to large-scale virus production

Complete Kit– Including filtration devices, AdenoPACK

units for virus purification, Vivaspin® and all buffers

Low Endotoxin Levels– High cell viability and infection rates due

to endotoxin levels of < 0.025 EU/ml

Adenovirus Purification with Vivapure® AdenoPACK Kits

Specifications

Adenovirus Purification Kit Specifications

Product AdenoPACK 20 AdenoPACK 100 AdenoPACK 500Sample size 20 ml cell culture 20 ml to 200 ml of

cell culture500 ml of cell culture

Number of purifications

6 + 20 ml 2 + 20 ml to 60 ml 1 + 200 ml

1 + 500 ml

Virus particles (VP) per ml

Typically up to 1 + 1011-1012

Typically up to 1 + 1013


VP/IU 50 to 100 20 to 50 20 to 50Processing time Typically one hour Typically two hoursEndotoxin level <0.025 EU/ml <0.025 EU/ml <0.025 EU/ml



Vivapure® AdenoPACK 20Vivapure® AdenoPACK 20 VS-AVPQ020Vivapure® AdenoPACK 20 RT* VS-AVPQ022

* AdenoPACK 20 RT does not contain Benzonase®**

Vivapure® AdenoPACK 100 Vivapure® AdenoPACK 100 VS-AVPQ101Vivapure® AdenoPACK 100 RT* VS-AVPQ102

AdenoPACK 100 AccessoriesVS-AVPA001 Pump tubing set for

Vivapure® AdenoPACK 100

* AdenoPACK 100 RT does not contain Benzonase®**

Vivapure® AdenoPACK 500 Vivapure® AdenoPACK 500 VS-AVPQ501Vivapure® AdenoPACK 500 RT* VS-AVPQ502

* AdenoPACK 500 RT does not contain Benzonase®**** Benzonase® is a registered trademark of Merck


LentiSELECT 40|500|1000The LentiSELECT kits for lentivirus purifica-tion and concentration offer researchers who need to recover up to 5 + 109 infective lentivirus particles per ml for in vitro transfection or animal studies a fast and easy-to-use solution.

These straightforward kits replace time-consuming ultracentrifugation protocols, which typically take approximately one day for large sample volumes. Vivapure® LentiSELECT thus reduces purification time to just a few hours.

LentiSELECT kits are offered as LentiSELECT 40, LentiSELECT 500 and LentiSELECT 1000 for the purification and concentration of VSV-G pseudotyped lentivirus from 40 ml to 1,000 ml cell cultures, resulting in 8 + 108 to 1 + 1010 purified infective particles. The most convenient handling method is provided for each sample volume. To this end, 40 ml sample volumes are processed manually with LentiSELECT 40, while LentiSELECT 500 and 1000 are pump-driven kits.

LentiSELECT Advantages

Fast and Easy Virus Purification– Purification completed in less than one to

six hours, depending on sample volume– Kit is as easy to use as filtration

No Need for Expensive Instruments– Lentivirus purification with LentiSELECT

is independent of equipment, such as ultracentrifuges

High Virus Purity– Achieve pure virus based on a

chromatographic method for your experiments instead of a crude and variable cell culture supernatant pellet

Optimal for Multiple Virus Construct ScreeningWith LentiSELECT 40, four purification runs can be conducted in parallel with one kit

Complete Kits– Including LentiSELECT units for virus

purification, Vivaspin® units for concentration| buffer exchange and all buffers and syringes necessary

Low Endotoxin Levels– High cell viability and infection rates due

to endotoxin levels of < 0.025 EU/ml

Lentivirus Purification with the Vivapure® LentiSELECT Kit

Specifications

Product LentiSELECT 40 LentiSELECT 500 LentiSELECT 1000Sample size 40 ml cell culture 500 ml of cell culture 1,000 ml of

cell cultureNumber of purifications

4 + 40 ml 1 + 500 ml 1 + 1,000 ml

Virus particles (VP) per ml


Typically up to 2-5 + 109*

Typically up to 4-6 + 1013

VP/IU 5 to 15 5 to 15 20 to 50Processing time Typically up to

45 minTypically up to 3 hours

Typically up to 6 hours

Endotoxin level <0.025 EU/ml <0.025 EU/ml <0.025 EU/ml



Vivapure® LentiSELECT 40Vivapure® LentiSELECT 40 VS-LVPQ040

Vivapure® LentiSELECT 500 Vivapure® LentiSELECT 500 VS-LVPQ500

Vivapure® LentiSELECT 1000 Vivapure® LentiSELECT 1000 VS-LVPQ1000

Introduction 57Filtration Devices

60 Minisart® Syringe Filters

76 Sartolab® P20 and Sartolab® P20 Plus

79 Sartolab® 150v

81 Sartolab® RF | BT

84 Chemical Compatibility

Filtration DevicesTable of Contents

58 Filtration Devices58 Introduction

Syringe filters are used for many routine preparation steps in laboratories all over the world. They are convenient, ready-to-use disposables for sterile filtration of liquids and removal of particles from solutions and gases. Depending on the reagents filtered, syringe filters have to fulfill certain requirements to best serve customer’s application. Sartorius offers Minisart® syringe filters and filters optimized for a wide range of relatively large volumes. The filters are clean and safe as they are virtually free of leachables and extractables and reliably remove particles and microorganisms with no leakage. If you need to rely on the quality of your filtrate – whether it needs to be sterile prior to injection or particulate-free before analysis – field-proven, high-quality Sartorius filter syringes are the No. 1 choice for reliable, convenient preparation steps.

Our Product RangeMinisart® syringe filters feature a housing made of medical-grade acrylic (MBS), which makes them the perfect choice for sterile filtration and clarification of additives, buffers, reagents, drugs and gases. Their effective filtration area of 6.2 cm2 for liquids is the largest among premium syringe filters. The combination of the MBS housing material and surfactant-free cellulose acetate, PES or PTFE membranes add comma provides the lowest non-specific binding. As a result, Minisart® delivers excellent performance in filtering medical drugs or gases for sterilization or particle removal prior to use.

59Filtration DevicesIntroduction

Minisart® syringe filters with a polypropylene housing are optimized for filtration prior to analysis and withstand even harsh solvents and chemicals. Due to the typical range of volumes from less than 1 ml to 100 ml, these filters are available in three different diameters with an effective filtration area of 0.07 cm2, 1.7 cm2 and 4.8 cm2 for the optimum filter capacity and the lowest hold-up volume.

Sartolab® vacuum filtration devices with 0.1 µm and 0.22 µm PES membranes for convenient filtration of 150 ml up to 1 L are ready to use and sterile. Sartolab® RF is a complete system that includes a receiver flask. Sartolab® BT Sterile is a bottle top filter without a receiver flasks. This enables customers to use a receiver bottle of their choice and to even expand filtration capacity, depending on the particle load of the filtered liquid by filling more than one receiver flask. Sartolab® 150V is a disposable vacuum filter with a pleated 0.22 µm PES membrane, which is suitable for up to 15 L of liquid.

Sartolab® pressure filtration devices with a 0.2 µm SFCA or PES membrane are available with or without a glass fiber prefilter, depending on your needs. Sartolab P20 is designed for up to 3 L volumes and can also be used in-line. The polycarbonate housing and membrane components are ideal for filtering liquid medical drugs. The glass fiber prefilter types are ideal for filtering environmental samples that have a high particle load prior to analyzing such samples.

Typical Applications for Filtration Devices– Sterile filtration of liquids and gases with virtually no effect on

the ingredients– Particle removal from liquids and gases prior to downstream processes– Venting of vials, bottles, containers, bags and bioreactors and fermenters– Removal of precipitates and coagulates from medical solutions prior to use

60 Filtration Devices60 Minisart® Syringe Filters

Minisart® Syringe FiltersRemoval of Particles and Microorganisms from Liquids and Gases

Sample Preparation HPLC | UHPLC | AnalyticsElimination of particles from your samples prior to HPLC or other chromatographic analysis is essential in order to maintain the integrity of your chromatography column and to maximize its operating lifetime. Minisart® syringe filters optimized for sample preparation consist of a

Sartorius offers Minisart® syringe filters for a wide range of applications. The filters are clean and safe as they are virtually free of leachables and extractables. In addition, they reliably remove particles and microorganisms – without any leakage. Minisart® with a polypropylene housing is optimized for filtration prior to analysis and withstands even harsh solvents and chemicals. Minisart® with a housing made of medical-grade acrylic (MBS) is the perfect choice for sterile filtration and clarification of additives, buffers, reagents, drugs and gases.

Filtration of Aqueous Liquids Clarification | Sterile FiltrationFor clarification and sterilization of liquids, filtration is the optimal method. It removes all microorganisms and particles reliably, without any effects on the ingredients due to adsorption or decomposition. For optimal results, Minisart® syringe filters with an MBS housing provide a choice of

Medical Use and Special Sterile Venting ApplicationsMinisart® syringe filters are ideal for clarification of liquids laden with particles, e.g. for preparation of pharmaceuticals or infusion solutions. For sterilization and removal of particles from air and other gases, syringe filters are optimal for

polypropylene housing and membrane components featuring maximum chemical compatibility and minimum extractables to ensure excellent results. Due to the typical range of volumes from less than 1 ml to 100 ml, these filters are available in three different diameters with an effective filtra-tion area of 0.07 cm2, 1.7 cm2 and 4.8 cm2. For a selection guide, please see page 61.

membranes with pore sizes ranging from 0.1 µm to 5 µm for high flow rates and the lowest adsorption characteristics. The effective filtration area of 6.2 cm2 for the fastest filtration is the largest among premium syringe filters available, and the MBS housing is color-coded for easy pore size identification. For a list of the types offered, please see page 65.

sterile venting of containers, bioreactors, fermenters and tubing systems in medical devices. Many Minisart® syringe filters have the CE mark of con formity (European directive) and are available with a wide choice of membranes, connectors and housing materials. For a list of the types offered, please see page 68.

A Full Range of Filters Dedicated for Various Filtration Applications

61Filtration DevicesMinisart® Syringe Filters

Minisart® Selection GuidePlease refer to Minisart® RC, NY or SRP for the highest chemical compatibility on page 63. Please refer to Minisart® NML or Minisart® High Flow on page 66. Several additional Minisart® units for venting and special purposes are shown on page 70.

Prefiltration

Sam

ple

Com

posi

tion

Pore

Siz

esSa

mpl

e Vo

lum

es

SFCASurfactant-FreeCellulose Acetate

28 mm for up to 200 ml

PES Polyethersulfone

0.2 µm


All Aqueous SolutionsTissue Culture Media

UHPLC, etc.(Columns < 3 µm Particles) Bacteria

1 ml to 100 ml

RC RegeneratedCellulose

0.45 µm


NY Polyamide, Nylon

0.65 µm

PTFE Polytetrafluoroethylene

0.8 µm 1.2 µm 5 µm

4 mm for up to 1 ml

Aqueous | SolventMixtures | Solvents

HPLC, etc.(Columns > 3 µmParticles) Particles

ParticlesYeast Cells

ParticlesYeast Cells

ParticlesYeast CellsPlatelets

Large ParticlesGrit, Cells

0.5 ml to 15 ml

Solvent Mixtures |Solvents

Aqueous | Solvents

Sample Preparation | Clarification | Particle Removal

All Aqueous SolutionsBuffers, Protein Analysis

Small BacteriaMycoplasmaColloids > 0.1

1 ml to 200 ml

Solvents | Gases | Acids | Bases

0.05 ml to 1 ml

Aqueous

Sterilization

Hydrophilic Hydrophobic

Glass PrefilterGlass+MembraneHighly Particle-laden Samples

GF (Glass Fiber)0.1 µm

62 Filtration Devices62

min0 10 20 30 40 50 60

mAU

-10

010

20

30

40

50

60

70

min0 10 20 30 40 50 60

mAU

-10

010

20

30

40

50

60

70

min0 10 20 30 40 50 60

mAU

-10

010

20

30

40

50

60

70

Filtered with Minisart® PES Filtered with Minisart® NML Unfiltered Water

Water

Filtered with Minisart® SRP Filtered with Minisart®NY Filtered with Minisart® RC Unfiltered Methanol

Methanol

Acetonitrile Filtered with Minisart® SRP Filtered with Minisart®NY Filtered with Minisart® RC Unfiltered Acetonitrile

Minisart® Syringe Filters

Sample Preparation for Analytical ProceduresReliable Removal of Particles and Microorganisms from Liquids and Gases

Particle removal by filtration before analysis substantially increases the lifetime of your columns. Minisart® RC is optimized for aqueous liquids and solvents and is compatible with DMSO, other amides, ketones, esters and ethers. Minisart® NY is exceptionally pure compared with other common polyamide (nylon) filters and competitor products. For this product raw materials are used which do not interfere with standard analytical methods.

Our coating-free hydrophobic PTFE membrane used in Minisart® SRP is suitable for venting applications and leachables-free clarification of exceptionally harsh chemicals.

Minisart® Features– Low adsorption of analytes– Maximum chemical compatibility– Minimum extractables or leachables– Bidirectional use possible– 100% optical integrity test

HPLC Certification

HPLC ProcedureColumn C18: 250 × 4.6 mm, Flow Rate: 1 ml/min, Wavelength: 220 nmHPLC Injection Volume: 20 µl, Analysis Time: 65 min, Temperature: 40°C, Mobile Phases: A) Acetonitrile | B) Water, Gradient: Hold 60% A for 10 min, 60% to 95% A in 20 min, 95% to 100% A in 35 min

Minisart® SRP 15 mm

Minisart® RC 25 mm

Minisart® NY 25 mm

Minisart® SRP 25 mm

Minisart® NY 15 mm

Minisart® RC 15 mm

Male Luer Slip Outlet

Male Spike Outlet

4 mm packages are color-coded


Sample Preparation for Chromatography

Ordering InformationDia. in mm | EFA1 Membrane Housing Pore Size Connector Outlet Color | Printing Sterile* Qty./Pkg. Order No. Minisart® RC (Regenerated Cellulose)25 mm RC PP 0.2 µm Male Luer Slip White, Printed Yes 50 17764--------ACK

25 mm RC PP 0.2 µm Male Luer Slip White, Printed No 50 17764----------K

25 mm RC PP 0.2 µm Male Luer Slip White, Printed No 200 17764----------S

25 mm RC PP 0.2 µm Male Luer Slip White, Printed No 500 17764----------Q


25 mm RC PP 0.45 µm Male Luer Slip White, Printed No 200 17765----------S


15 mm RC PP 0.2 µm Male Luer Slip White, Printed Yes 50 17761--------ACK





4 mm RC PP 0.2 µm Male Luer Slip Blue Tray No 50 17821----------K

4 mm RC PP 0.2 µm Male Luer Slip Blue Tray No 500 17821----------Q

4 mm RC PP 0.45 µm Male Luer Slip Yellow Tray No 50 17822----------K

4 mm RC PP 0.45 µm Male Luer Slip Yellow Tray No 500 17822----------Q

Minisart® SRP (Hydrophobic PTFE)25 mm PTFE PP 0.2 µm Male Luer Slip White, Printed Yes 50 17575--------ACK

25 mm PTFE PP 0.2 µm Male Luer Slip White, Printed No 50 17575----------K

25 mm PTFE PP 0.2 µm Male Luer Slip White, Printed No 200 17575----------S

25 mm PTFE PP 0.2 µm Male Luer Slip White, Printed No 500 17575----------Q


25 mm PTFE PP 0.45 µm Male Luer Slip White, Printed No 200 17576----------S


15 mm PTFE PP 0.2 µm Male Spike White, Printed No 50 17558----------K

15 mm PTFE PP 0.2 µm Male Spike White, Printed No 500 17558----------Q

15 mm PTFE PP 0.2 µm Male Luer Slip White, Printed Yes 50 17573--------ACK



15 mm PTFE PP 0.45 µm Male Spike White, Printed No 50 17559----------K

15 mm PTFE PP 0.45 µm Male Spike White, Printed No 500 17559----------Q



4 mm PTFE PP 0.2 µm Male Luer Slip Blue Tray No 500 17844----------Q

4 mm PTFE PP 0.45 µm Male Luer Slip Yellow Tray No 50 17820----------K

4 mm PTFE PP 0.45 µm Male Luer Slip Yellow Tray No 500 17820----------Q


Dia. in mm | EFA1 Membrane Housing Pore Size Connector Outlet Color | Printing Sterile* Qty./Pkg. Order No. Minisart® NY (Nylon) and NY25 Plus (Glass Fiber 0.7 µm2 + Nylon)25 mm Nylon PP 0.2 µm Male Luer Slip White, Printed Yes 50 17845--------ACK

25 mm Nylon PP 0.2 µm Male Luer Slip White, Printed No 500 17845----------Q

25 mm Nylon PP 0.45 µm Male Luer Slip White, Printed Yes 50 17846--------ACK

25 mm Nylon PP 0.45 µm Male Luer Slip White, Printed No 500 17846----------Q

15 mm Nylon PP 0.2 µm Male Luer Slip White, Printed No 50 1776B----------K

15 mm Nylon PP 0.2 µm Male Luer Slip White, Printed No 500 1776B----------Q

15 mm Nylon PP 0.45 µm Male Luer Slip White, Printed No 50 1776C----------K

15 mm Nylon PP 0.45 µm Male Luer Slip White, Printed No 500 1776C----------Q

25 mm GF+Nylon PP 0.2 µm Male Luer Slip White, Printed No 50 1784B----------K

25 mm GF+Nylon PP 0.2 µm Male Luer Slip White, Printed No 500 1784B----------Q

25 mm GF+Nylon PP 0.45 µm Male Luer Slip White, Printed No 50 1784C----------K

25 mm GF+Nylon PP 0.45 µm Male Luer Slip White, Printed No 500 1784C----------Q

* Sterile Minisart® syringe filters are individually packaged. If not stated otherwise, Minisart® units have been sterilized by ethylene oxide. Non-presterilized Minisart® units: RC, PTFE and nylon can be sterilized by autoclaving at 121°C for 30 min/or by using ethylene oxide (EO).

1 Diameter of EFA – Effective Filtration Area2 0.7 µm = GF particle retention = pore size!

For technical product specifications, please see page 72.

65Filtration Devices

0

217

262240

165

223

Min

isar

t® PES

Com

peti

tor

A

Com

peti

tor

B

Com

peti

tor

C

Com

peti

tor

D

ml/m

in a

t 1

bar

Minisart® Syringe Filters

0

Min

isar

t® SFC

A

Com

peti

tor

A

Com

peti

tor

B

Com

peti

tor

C

163

255

204

145ml/m

in a

t 1

bar

0.45 µm SFCA membrane compared with CA (Cellulose Acetate) or similar hydrophilic membranes of the same pore size

0.45 µm PES membrane compared with other PES membranes of the same pore size

Filtration of Aqueous Liquids – Clarification | SterilizationFiltration Is the Optimal Method for Clarification and Sterilization of Liquids

Sterilization by filtration is the fastest method for removal of bacterial cells from liquids, while minimizing the effects on ingredients. Minisart® NML with surfactant-free cellulose acetate (SFCA) is the best choice for all aqueous solutions with a pH of 4 to 8. It combines fast flow rates with an exceptionally pure and leachables-free membrane and is available in many different pore sizes – also for the removal of larger particles. Minisart® High Flow with polyethersulfone (PES) is optimal for delivering the highest flow rates and for a broad pH compatibility range from 1 to 13. Due to the asymmetric membrane structure, the PES surface virtually behaves like a prefilter. Both Minisart® types – NML and High Flow – can be sterilized by

ethylene oxide (EO) or gamma irradiation and are suitable for gluing and assembling on tubing. In addition, Minisart® NML has the CE mark of conformity.

Minisart® Features– The largest effective filtration area (EFA)

of 6.2 cm2

– The lowest adsorption– Superior flow rate– High total throughput– Low hold-up volume– Minimum extractables– PVC-free– Gamma-irradiated or EO-sterilized– Bidirectional use possible– 100% optical integrity test

Water Flow Rates at 1 bar (ml/min); 15.4 psi; 0.45 µm Hydrophilic Membranes

0.22

0.8

0.2

0.45

1.2

0.45

0.1

5.0

0.65

Minisart® High Flow with PES

Minisart® NML with SFCA

28 mm EFA33 mm housing diameter


Preparation of Aqueous Liquids

Ordering InformationDia. in mm | EFA1

Membrane Housing Pore Size

Connector Outlet

Color | Printing

Sterile* Qty./Pkg.

Order No. CE Marking

Minisart® High Flow (PES – Polyethersulfone)

28 mm PES MBS 0.1 µm Male Luer Lock Dark Red Yes 50 16553----------K

28 mm PES MBS 0.22 µm Male Luer Lock Royal Blue Yes# 50 16532--------GUK

28 mm PES MBS 0.22 µm Male Luer Lock Royal Blue Yes 50 16532----------K

28 mm PES MBS 0.22 µm Male Luer Slip Royal Blue Yes 50 16541----------K

28 mm PES MBS 0.22 µm Male Luer Lock Royal Blue No 500 16532----------Q

28 mm PES MBS 0.22 µm Male Luer Slip Royal Blue No 500 16541----------Q

28 mm PES MBS 0.45 µm Male Luer Lock Amber Yes 50 16537----------K

28 mm PES MBS 0.45 µm Male Luer Lock Amber No 500 16537----------Q

28 mm PES MBS 0.45 µm Male Luer Slip Amber Yes# 50 16533--------GUK

28 mm PES MBS 0.45 µm Male Luer Slip Amber Yes 50 16533----------K

28 mm PES MBS 0.45 µm Male Luer Slip Amber No 500 16533----------Q

Minisart® NML (SFCA – Surfactant-free Cellulose Acetate)

28 mm SFCA MBS 0.2 µm Male Luer Lock Blue Yes 50 16534----------K CE-marked

28 mm SFCA MBS 0.2 µm Male Luer Lock Blue Yes# 50 16534--------GUK CE-marked

28 mm SFCA MBS 0.2 µm Male Luer Lock Blue No 500 16534----------Q CE-marked

28 mm SFCA MBS 0.2 µm Male Luer Slip Blue Yes 50 17597----------K CE-marked

28 mm SFCA MBS 0.2 µm Male Luer Slip Blue No 500 17597----------Q CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Lock Yellow Yes 50 16555----------K CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Lock Yellow Yes# 50 16555--------GUK CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Lock Yellow No 500 16555----------Q CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Slip Yellow Yes 50 17598----------K CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Slip Yellow No 500 17598----------Q CE-marked

28 mm SFCA MBS 0.65 µm Male Luer Slip Pink Yes 50 16569----------K

28 mm SFCA MBS 0.8 µm Male Luer Lock Green Yes 50 16592----------K

28 mm SFCA MBS 0.8 µm Male Luer Lock Green Yes# 50 16592--------GUK

28 mm SFCA MBS 0.8 µm Male Luer Lock Green No 500 16592----------Q

28 mm SFCA MBS 1.2 µm Male Luer Lock Red Yes 50 17593----------K

28 mm SFCA MBS 1.2 µm Male Luer Lock Red No 500 17593----------Q

28 mm SFCA MBS 5 µm Male Luer Lock Brown Yes 50 17594----------K CE-marked

28 mm SFCA MBS 5 µm Male Luer Lock Brown No 500 17594----------Q


Dia. in mm | EFA1


Connector Outlet

Color | Printing

Sterile* Qty./Pkg.


Minisart® NML Plus (Glass Fiber 0.7 µm2 + SFCA)

28 mm GF+SFCA MBS 0.2 µm Male Luer Lock Blue Yes 50 17823----------K

28 mm GF+SFCA MBS 0.2 µm Male Luer Lock Blue No 500 17823----------Q

28 mm GF+SFCA MBS 0.45 µm Male Luer Lock Yellow Yes 50 17829----------K

28 mm GF+SFCA MBS 0.45 µm Male Luer Lock Yellow No 500 17829----------Q

28 mm GF+SFCA MBS 1.2 µm Male Luer Lock Red No 500 17825----------Q

28 mm GF MBS 0.7 µm2 Male Luer Lock White No 50 17824----------K

28 mm GF MBS 0.7 µm2 Male Luer Lock White No 500 17824----------Q

* Sterilized Minisart® units are individually packaged. If not stated otherwise, Minisart® are sterilized by ethylene oxide. #-Mark indicates sterilization by gamma irradiation. Non-presterilized Minisart® units: PES, SFCA, GF+SFCA and GF can be sterilized by ethylene oxide or gamma irradiation.

1 Diameter of EFA – Effective Filtration Area2 0.7 µm = GF particle retention = pore size!

For technical product specifications, please see page 74.


Special Applications – Medical Uses and Sterile VentingChoose from a Broad Range of Pore Sizes, Materials and Formats

Bacterial cell removal or particulate removal from liquids, including medical drugs, can easily be performed with Minisart® syringe filters. Minisart® has a minimal effect on the ingredients of a filtered solution. Minisart® NML and Ophthalsart with surfactant-free cellulose acetate (SFCA) and Minisart® HY and SRP with hydro phobic PTFE have the CE mark of conformity and are frequently used for sterile filtration of aqueous and oily ear or eye solutions and other drugs. Minisart® NML with a 5 µm pore size removes particulates or coagulates in dissolved medical drugs prior to injection, offering the highest total throughput under sterile conditions – without any early

clogging. Hydrophobic PTFE filters are suitable for venting purposes and are additionally available in special formats with activated carbon.

Minisart® Features– 100% optical integrity test– Low adsorption– Minimum extractables– Particulate-free– PVC-free– Gamma-irradiated or EO-sterilized– Many CE-marked types– Bidirectional use possible– Many configurations available

Pressure-Hold Test

Method: Pressure-hold tests were performed by connecting pre-wetted syringe filter units to a 10-fold pressure device with a pressure gauge. The pressure applied equaled 4/5 of the bubble point. Units failed the test if they released air bubbles before reaching 4/5 of the minimum bubble point. These units were remeasured to determine at which pressure air bubbles appear.Minisart® available in many

configurations

Result: Testing the pressure-hold capability of syringe filters revealed that many competitor 3’s filters are dysfunctional and not intact. Filtration performed with such filters will result in unsterile filtrates or filtrates without an appropriately reduced level of particles.

Tested Syringe Filter Units

Pres

sure

in b

ar 3

2.5

2

1.5

1

0.5

0

302515 2051 10

Minisart® PESMinisart® CACompetitor 1 PESCompetitor 2 PESCompetitor 3 PESCompetitor 3 CA


Biocompatibility Certificate Minisart® HY

Biocompatibility Certificate Minisart® NML

Declaration of Conformity Minisart®


Minisart® Syringe Filters – Special

Ordering InformationDia. in mm | EFA1


Connector Outlet

Color | Printing

Sterile* Qty./Pkg.


Minisart® NML (SFCA – Cellulose Acetate) Aqueous Filtration

28 mm SFCA MBS 0.2 µm Male Luer Lock Blue Yes 50 16534----------K CE-marked

28 mm SFCA MBS 0.2 µm Male Luer Lock Blue Yes# 50 16534--------GUK CE-marked

28 mm SFCA MBS 0.2 µm Male Luer Lock Blue No 500 16534----------Q CE-marked

28 mm SFCA MBS 0.2 µm Male Luer Slip Blue Yes 50 17597----------K CE-marked

28 mm SFCA MBS 0.2 µm Male Luer Slip Blue No 500 17597----------Q CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Lock Yellow Yes 50 16555----------K CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Lock Yellow Yes# 50 16555--------GUK CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Lock Yellow No 500 16555----------Q CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Slip Yellow Yes 50 17598----------K CE-marked

28 mm SFCA MBS 0.45 µm Male Luer Slip Yellow No 500 17598----------Q CE-marked

28 mm SFCA MBS 5 µm Male Luer Lock Brown Yes 50 17594----------K CE-marked

Minisart® Ophthalsart (SFCA – Cellulose Acetate) Aqueous Filtration

28 mm Ophthalsart MBS 0.2 µm Male Luer Slip Pink Yes 50 17528----------K CE-marked

Minisart® High Flow (PES – Polyethersulfone) Aqueous Filtration

28 mm PES MBS 0.1 µm Male Luer Lock Dark Red Yes 50 16553----------K

Minisart® PES (Polyethersulfone) Aqueous Filtration

15 mm PES PP 0.22 µm Male Luer Slip White Yes 50 1776D--------ACK

Minisart® Air (Hydrophobic PTFE) Venting

15 mm PTFE MBS 0.2 µm Male Luer Slip Yellow No 500 1751A----------Q

15 mm PTFE MBS 0.2 µm Male Luer Slip + Needle

Yellow Yes# 50 16596--------HNK

Minisart® HY (hydrophobic PTFE), CE-Marked, for Venting and Gas Filtration

26 mm PTFE MBS 0.2 µm Male Luer Lock Clear Yes 50 16596--------HYK CE-marked

26 mm PTFE MBS 0.2 µm Male Luer Lock Clear No 500 16596--------HYQ CE-marked

26 mm PTFE MBS 0.2 µm Male Luer Locka Clear No 500 16599--------HYQ CE-marked

26 mm PTFE MBS 0.2 µm Hose Barbsb Clear No 500 40078----------Q CE-marked

26 mm PTFE MBS 1 µm Male Luer Lock Clear No 500 1659A--------HYQ

26 mm PTFE MBS 1 µm Hose Barbsb Clear No 500 1659B--------HYQ

Minisart® Acticosart with Dome Reservoir and Hydrophobic PTFE for Venting and Ultracleaning of Gases26 mm Active

CarbonMBS 0.45 µm Male Luer Slipa Blue No 500 17840----------Q


Dia. in mm | EFA1


Connector Outlet

Color | Printing

Sterile* Qty./Pkg.


Minisart® SRP (Hydrophobic PTFE) CE-marked Venting & Gas Filtration

25 mm PTFE PP 0.2 µm Male Luer Slip White, Printed

Yes 50 17575--------ACK CE-marked

25 mm PTFE PP 0.2 µm Hose Barb White No 500 1757A----------Q

* Sterilized Minisart® units are individually packaged. If not stated otherwise, Minisarts are sterilized by ethylene oxide. #-mark indicates sterilization by gamma irradiation Non-presterilized Minisart® units: SFCA can be sterilized by ethylene oxide or gamma irradiation. PTFE can be sterilized by ethylene oxide.

a Connector inlet: Male Luer slip (all other Minisart® types have female luer lock inlets) b Hose barbs, inlet and outlet, 5 mm diameter1 Diameter of EFA – Effective Filtration Area

For technical product specifications, please see pages 72 and 74.

Please contact us to learn more about additional Minisart® configurations that are available.

Do you need other quantities per package?Are you looking for special versions or do you have requirements?Do you need other inlet and | or outlet connectors?


Minisart® with Polypropylene Housing

SpecificationsMinisart® RC | SRP | NY | PES with 4 | 15 | 25 mm Diameter Membrane Filtration AreaHousing material Polypropylene (PP)

Membranes RC = Regenerated Cellulose | NY = Polyamide | SRP = Hydrophobic PTFE = Polytetrafluoroethylene | PES = Polyethersulfone

Glass fiber prefilter NY Plus: Ultrapure quartz, 0.7 µm particle retention

Application limits Max. recommended operating pressure 4.5 bar | 65 psi

Housing burst pressure > 7 bar | 102 psi

Max. temperature 121°C, 30 min (autoclavable)

Sterilization Non-sterile Minisart® can be autoclaved or sterilized by ethylene oxide (EO)

Minisart® Membrane Types RC 0.2 µm RC 0.2 µm RC 0.45 µm SRP 0.2 µm SRP 0.45 µmNon-sterile packs: 50 (K), 200 (S), 500 (Q), 1000 (R) | sterile packs: individual packaged, 50 (ACK)

K | S | Q | R ACK K | S | Q | R K | S | Q | ACK K | S | Q

Bubble point (>) With water 3.0 bar | 44 psi

With water 4.6 bar | 67 psi


With ethanol 1.4 bar | 20 psi


Flow rate, 4 mm dia. = 0.07 cm2 filter area | Hold-up volume1: 5 µl to 10 µl (R ml/min)

– For water at 1 bar 0.5 – 1.5 –3 –3

– For methanol at 1 bar 1.5 – 3.0 2.0 4.5

– For air at 0.1 bar –2 – –2 30 60


– For water at 1 bar 20 10 40 –3 –3

– For methanol at 1 bar 55 25 105 55 150

– For air at 0.1 bar –2 –2 –2 800 1600


– For water at 1 bar 80 50 160 –3 –3

– For methanol at 1 bar 160 90 325 160 260

– For air at 0.1 bar –2 –2 –2 1800 3000

Water penetration point – – – > 4.0 bar | 58 psi3 > 3.0 bar | 44 psi3

Sterile filtration capability acc. to the bacteria challenge text

No5 Yes No Yes No

Pyrogen-free according to the USP

Yes

Cytotoxity (17575-ACK) No inhibition with MRC-5 (human lung cells) and L929


Minisart® Membrane Types NY 0.2 µm NY 0.45 µm NY Plus 0.2 µm NY Plus 0.45 µm PES 0.2 µmNon-sterile packs: 50 (K), 200 (S), 500 (Q), 1000 (R) | sterile packs: individual packaged, 50 (ACK)

K | Q | R | ACK K | Q | R | ACK K | Q K | Q K | Q | ACK







– For water at 1 bar – – – – 1.5

– For methanol at 1 bar – – – – –4

– For air at 0.1 bar – – – – –2


– For water at 1 bar 20 40 – – 40

– For methanol at 1 bar 40 110 – – –4

– For air at 0.1 bar –2 –2 – – –2


– For water at 1 bar 50 100 50 100 100

– For methanol at 1 bar 70 200 70 200 –4

– For air at 0.1 bar –2 –2 –2 –2 –2

Water penetration point – – – – –

Sterile filtration capability acc. to the bacteria challenge test

Yes No Yes No Yes

Pyrogen-free according to the USPCytotoxity (17575-ACK) No inhibition with MRC-5 (human lung cells) and L929

1 Hold-up volume after air purge2 Hydrophilic membranes can filter dry air or gas but become impermeable to air or gas when wetted!3 Hydrophobic membranes cannot be wetted with aqueous solutions unless you overcome their water penetration point or pre-wet them using

an organic solvent (e.g. ethanol)4 PES is suitable for solutions only containing up to 30% MeOH5 According to the bacterial challenge test (BCT) with 107 Brevundimonas diminuta. Non-sterile RC Minisart® types are optimized for sample

preparation and are not suitable for sterile filtration according to the bacteria challenge test. All other non-sterile Minisart® types with 0.2 mm pore size can be sterilized by autoclaving or EO before use for sterile filtration.


Minisart® with MBS Housing

SpecificationsMinisart® High Flow | NML | NML Plus with 28 mm accessible membrane filtration area diameter, 100 µl to 150 µl hold-up volume1 Minisart® HY | Acticosart with 26 mm accessible membrane filtration area diameter, 100 µl to 150 µl hold-up volume1 Minisart® Air with 15 mm accessible membrane filtration area diameter, 100 µm hold-up volume1

Housing material Methacrylate butadiene styrene (MBS)

Membranes High Flow: PES = Polyethersulfone NML: (SF) CA = (Surfactant-free) Cellulose Acetate NML Plus: (SF) CA = (Surfactant-free) Cellulose Acetate HY | Acticosart | Air: Hydrophobic PTFE = Polytetrafluoroethylene

Glass fiber pre-filter NML Plus: Binder-free GF, 0.7 µm particle retention

Application limits High Flow: Max. recommended operating pressure 6.0 bar | 87 psi NML, NML Plus, HY, Air: Max. recommended operating pressure 4.5 bar | 65 psi Acticosart: Max. recommended operating pressure 1 bar | 14.5 psi

Housing burst pressure > 7 bar | 102 psi (not determined for Acticosart)

Max. temperature 60°C, not autoclavable

Sterilization Non-sterile Minisart® High Flow, NML and NML Plus can be or sterilized by ethylene oxide (EO) or by gamma sterilizationNon-sterile Minisart® HY, Acticosart, Air* can be sterilized by ethylene oxide (EO)

Minisart® Membrane Types PES 0.1 µm

PES 0.2 µm

PES 0.45 µm

CA 0.2 µm

CA 0.45 µm

CA 0.65 µm

CA 0.8 µm

CA 1.2 µm

CA 5.0 µm

Non-sterile packages: 500 (Q, HYQ), 1000 (R), sterile packs: individually packaged, 50 (K, GUK, HYK, HNK)

K K | GUK | Q

K | GUK | Q

K | GUK | Q

K | GUK | Q

K K | GUK | Q

K | Q K | Q









With water0.4 bar | 6 psi

Flow Rate for 2 | 3 (R ml/min)

28 mm dia. for water at 1 bar 40 140 220 60 180 250 400 500 600

15 mm dia. for air at 0.1 bar – – – – – – – – –

26 mm dia. for air at 0.1 bar – – – – – – – – –

Water penetration point – – – – – – – – –

Sterile filtration capability4 acc. to the bacteria challenge test

Yes Yes No Yes No No No No No


Yes Yes Yes

Cytotoxity No inhibition with MRC-5 (human lung cells) and L929


Minisart® Membrane Type GF + CA 0.2 µm

GF + CA 0.45 µm

GF + CA 1.2 µm

GF 0.7 µm

PTFE 0.2 µm

PTFE 1.0 µm

Acticosart PTFE (Air) 0.2 µm

Non-sterile packages: 500 (Q, HYQ), 1000 (R), sterile packs: individually packaged, 50 (K, GUK, HYK, HNK)

K | Q K | Q Q K | Q HYK | HYQ | Q

HYQ Q Q | HNK









Flow rate for2 | 3 (R ml/min)

28 mm dia. for water at 1 bar 60 160 350 450 – – – –

15 mm dia. for air at 0.1 bar – – – – – – – 2000

26 mm dia. for air at 0.1 bar – – – – 2000 4000 2300 –

Water penetration point – – – – > 4.0 bar | 58 psi3

> 1.5 bar | 22 psi3

N.a. > 3.0 bar | 44 psi3

Sterile filtration capability4 according to the bacteria challenge test

Yes No No No Yes No N.a. Yes


Yes

Cytotoxity No inhibition with MRC-5 (human lung cells) and L929

1 Hold-up volume after air purge2 Hydrophilic membranes can filter dry air or gas but become impermeable to air or gas when wetted!3 Hydrophobic membranes cannot be wetted with aqueous solutions unless you overcome their water penetration point.4 According to bacterial challenge test (BCT) with 107 Brevundimonas diminuta. All non-sterile Minisart® types listed above can be sterilized

according to the method recommended in this table.* Minisart® Air can be sterilized by gamma rays according to the following parameters: Range 25 – 40 kGy (validated with 50 kGy).

76 Filtration Devices76 Sartolab® P20 and Sartolab® P20 Plus

Sartolab® P20 and Sartolab® P20 PlusSterile Filtration of Sample Volumes of Up to 5 Liters

Using Sartolab® P 20 or Sartolab® P 20 Plus in Available Systems

Systems with Luer Lock ConnectorsSartolab® units with a luer lock inlet fit directly onto the corresponding connectors of tubing from peristaltic pumps. This configuration can also be used with luer lock syringes.

Systems with TubingIf the liquid to be filtered will be pumped through open tubing from a pressure tank or a peristaltic pump, then a Sartolab® unit with a hose nipple inlet is required. The stepwise increase in diameter of the nipple, from 6 mm to 12 mm, makes it suitable for a wide range of tubing.

ApplicationThe Sartolab® P20 is a ready-to-use pressure filtration unit for sterile filtration of media and aqueous solutions in batches ranging from 100 ml to 5 l. For media that contain sera and difficult-to-filter solutions, a Sartolab® P20 Plus unit with an incorporated prefilter is also available.

SecurityThe effectiveness of every batch of 0.2 µm cellulose acetate membranes for sterile filtration is confirmed by bacteria challenge tests (HIMA) using Brevundimonas diminuta. Only biosafe

material is used in the filtration units; they have been proven non-toxic by passing the USP plastics test for toxicity. Tests with MRC-5 human lung cells on cellulose acetate membranes and glass fiber prefilters showed no cytotoxic effects. Finished units are also tested for their sterile filtration capability and for housing and membrane integrity.

Fast FiltrationThe combination of a large filtration area (20 cm2) and the optimal design of the filter support guarantees high flow rates with high total throughputs. Automatic venting of any trapped air through the PTFE membrane-protected vent ports ensures that the entire filter surface is used for effective filtration.

0

50

100

150

V (ml)

Time (sec.) 10 50 100 200

A

B

CD

Competitor product

Choice of inlet connector, hose nipple or luer lock

With or without binder-free glass fiber prefilter

Polycarbonate housing and surfactant-free cellulose acetate membrane filter

20 cm2 filter area, optimal flow path and automatic venting

Outlet side with protective filling bell

77Filtration DevicesSartolab® P20 and Sartolab® P20 Plus

Specifications

Technical Specifications for Sartolab® P20 and Sartolab® P20 Plus Units with SFCA Membrane

Properties Description

Sartolab® P20 18052 18053 Sartolab® P20 Plus 18056 18058

Filter material SFCA, type 12587, 0.2 µm pore size and PTFE SFCA, type 12587 0.2 µm pore size plus GF, 100% free of binding agents and PTFE

Housing material Polycarbonate Polycarbonate

Color code Transparent Transparent

Filter diameter 64 mm 64 mm

Connector inlet Connector outlet

Female luer lock or stepped hose nipple with 6 mm to 12 mm outer diameter Hose nipple


Filling bell Yes Yes

Filtration area 20 cm2 20 cm2

Hold-up volume before bubble point 1 ml Approx 1.5 ml

Housing burst pressure > 5 bar|72.5 psi > 5 bar|72.5 psi

Bubble point > 3.2 bar|46.4 psi > 3.2 bar|46.4 psi

Max. recommended inlet pressure 3 bar|43.5 psi 3 bar|43.5 psi

Flow rate for water > 250 ml/min at ∆p = 1 bar|14.5 psi > 250 ml/min at ∆p = 1 bar|14.5 psi

Filtration range 100 ml to max. 5 l 100 ml to max. 10 l

pH-range 4–8 4–8

Non-specific protein adsorption No loss of protein detectable (filtration of γ globulin, method acc. to Bradford)

< 80 µg/cm2 (filtration of γ globulin, method acc. to Bradford)

Sterilization EO sterilization EO sterilization

Biosafety Class VI Plastics Test Class VI Plastics Test

Operating instructions Directions for use included in each box Directions for use included in each box

Technical Specifications for Sartolab® P20 and Sartolab® P20 Plus Units with PES Membrane

Sartolab® P20 Plus 18068 Sartolab® P20 18075

Filter material PES, type 15407 MI 0.2 µm pore size plus GF, 100% free of binding agents and PTFE

PES, type 15407 MI 0.2 µm pore size

Housing material Polycarbonate Polycarbonate

Color code Transparent Transparent

Filter diameter 64 mm 64 mm

Connector inlet Connector outlet



Filling bell Yes No

Filtration area 20 cm2 20 cm2

Hold-up volume before bubble point Approx 1.5 ml 1 ml

Housing burst pressure > 5 bar|72.5 psi > 5 bar|72.5 psi

78 Filtration Devices78 Sartolab® P20 and Sartolab® P20 Plus

Properties Description

Sartolab® P20 Plus 18068 Sartolab® P20 18075Bubble point > 3.2 bar|46.4 psi > 3.2 bar|46.4 psi

Max. recommended inlet pressure 3 bar|43.5 psi 3 bar|43.5 psi

Flow rate for water 400 ml/min at ∆p = 1 bar|14.5 psi 400 ml/min at ∆p = 1 bar|14.5 psi

Filtration range 100 ml–max. 10 l 100 ml–max. 5 l

pH range 1 to 8 1 to 8

Non-specific protein adsorption < 80 µg/cm2 (filtration of γ globulin, No loss of protein detectable (filtration of method acc. to Bradford) γ globulin, method acc. to Bradford)

Sterilization EO sterilization EO sterilization

Biosafety Class VI Plastics Test Class VI Plastics Test

Operating instructions Directions for use included in each box Directions for use included in each box


Type Membrane Housing Pore Size Inlet Outlet Sterile Qty./Pkg. Order No.Sartolab® P20

SFCA PC 0.2 µm Hose Nipple Hose Nipple Yes 10 18052----------DSFCA PC 0.2 µm Luer Lock Hose Nipple Yes 10 18053----------DPES PC 0.2 µm Luer Lock Hose Nipple Yes 10 18075----------D

Sartolab® P20 PlusSFCA + GF PC 0.2 µm Hose Nipple Hose Nipple Yes 10 18056----------DSFCA + GF PC 0.2 µm Luer Lock Hose Nipple Yes 10 18058----------DPES + GF PC 0.2 µm Luer Lock Hose Nipple Yes 10 18068----------D

SFCA – Cellulose Acetate, PES – Polyethersulfone, GF – Glass Fiber Prefilter, PC – Polycarbonate

79Filtration DevicesSartolab® 150v

Sartolab® 150v Disposable PES Vacuum Filtration Unit for Volumes of Up to 15 Liters

DescriptionSartolab®150v is a disposable, sterile, ready-to-use membrane filter capsule for highest convenience. Sartolab®150v capsules are made of a unique hydrophilic polyethersulfone (PES) membrane providing outstanding total throughput, flow rate performance, low extractables and broadest chemical compatibility.

ApplicationsTypical applications include sterilizing-grade filtration of:– Biological fluids– Purified water– Media– Buffers

CompatibilityThe PES membrane is compatible with a pH range from 1 to 14, making Sartolab®150v ideal for filtration of solutions with either high or low pH values.

Microbiological RetentionSartolab®150v capsules rated to 0.2 µm arefully validated as sterilizing-grade filtersaccording to HIMA and ASTM F-838-05guidelines.

Quality ControlEach individual element is integrity tested by the bubble point and diffusion test methods before final assembly. Sartolab®150v capsules are designed, developed and manufactured in accordance with a ISO 9001:2000 certified quality management system.

PerformanceThe unique pleated filter construction, combined with the highly asymmetric pore structure of the PES membrane, provides excellent flow rates and superior total throughput performance, especially in comparison to conventional disc filter systems.

SpecificationsMaterials and DataFilter Polyethersulfone (PES)Support fleece Polypropylene fleeceHousing PolypropyleneVent PTFEPore size 0.2 µmFiltration area 150 cm2

Flow rate 1.5 L/minTotal throughput 15 L

Ordering InformationQty./Pkg. Order No.3 18080-M

80 Filtration Devices80 Sartolab® 150v

To fill multiple receiving bottles, break the vacuum by opening the valve on top of the Sartolab®150v housing.

To continue filtration with the next receiving bottle, close the valve.

Reservoir

Place Sartolab®150v on the bottle neck. Vacuum is automatically created when used with a water jet pump or a different vacuum source.

Easy to Use

Vacuumsource

Create vacuum to start filtration.

81Filtration DevicesSartolab® RF | BT

Sartolab® RF | BT Disposable Filter Systems and Bottle-Top Filters

Sartolab® single-use sterile filter systems and bottle-top filters are designed for the vacuum filtration of tissue culture media and components, biological fluids, and other aqueous solutions.

The Sartolab® single-use 150 mL, 250 mL, 500 mL and 1,000 mL bottles are designed as storage containers for sterile media, buffers, or other aqueous solutions.

These products are for laboratory use only and not for human parenteral applications.

MaterialsThe filter funnels, dust covers and receiver bottles are manufactured from virgin, heavy metal-free polystyrene. The tubing adapters, filter adapters, and the plug seal caps are made of heavy metal-free polyethylene. Sartolab® filter systems are available with polyethersulfone membranes. All units are sterilized by gamma irradiation.

PerformanceThe filter units contain membranes integrally sealed to a support grid designed to maximize flow and reduce foaming and protein denaturation.

Specifications

Pore Size Membrane Material Characteristics0.22 µm Polyethersulfone Very low protein binding and low

extractables, fast flow rate0.1 µm Polyethersulfone Very low protein binding and low

extractables, fast flow rate

The membrane is compatible with most aqueous solutions and tested for use in cell culture applications.

Filter SystemsThe filter adapter utilizes a gasket design to ensure a vacuum-tight seal on the receiver|storage bottle. Each filter unit also contains a convenient tubing adapter that will fit most vacuum hoses.

The bottles are single-use containers. They cannot withstand autoclaving or use at temperatures greater than 70°C. The suitability of the bottles for storage of solutions below 0°C depends both on the solution and the storage conditions. Many aqueous solutions, including culture media, have been successfully frozen and stored at temperatures down to –20°C. However, a trial run under actual conditions is strongly recommended to test the suitability of the bottles for frozen storage.

82 Filtration Devices82 Sartolab® RF | BT

Bottle-Top FiltersThe filter adapter is available with a 45 mm thread finish, and is designed to ensure a vacuum-tight seal on customer-supplied bottles with the appropriate thread finish. Each filter unit also contains a tubing adapter that will fit most vacuum hoses.

Chemical CompatibilityThe mechanical strength, color, appearance, and dimensional stability of filter systems, bottle-top filters and plastic bottles are affected to varying degrees by the chemicals with which they come in contact. Specific operating conditions, especially temperature, will also affect their chemical resistance. A table is provided to serve as a general guideline for the chemical resistance of Sartolab® single-use sterile filters and bottles.

Chemical Resistance of Sartolab® Filters

Chemical Membrane Housing Class (PES) (PS) Weak Acids 3 1 Strong Acids 3 2 Alcohols 1 2 Aldehydes 3 3

Aliphatic Amines 1 3

Aromatic Amines 3 3

Bases 3 1

Esters 3 3

Hydrocarbons 3 3

Ketones 3 3

Key: 1. recommended 2. may be suitable for some applications; a trial run is recommended 3. not recommended. PS, polystyrene; PES, polyethersulfone.

83Filtration DevicesSartolab® RF | BT

Ordering Information Volume Membrane Filter Area Qty./Pkg. Order No. Sartolab® RF Filtration System Including Collection Bottle150 ml 0.22 µm PES 18 cm2 12 180C1----------E250 ml 0.22 µm PES 24 cm2 12 180C7----------E500 ml 0.22 µm PES 39 cm2 12 180C2----------E1,000 ml 0.22 µm PES 62 cm2 12 180C3----------E1,000 ml 0.1 µm PES 62 cm2 12 180C8----------E Sartolab® BT Bottle-Top Filters Without Integrated Collection Bottle150 ml 0.22 µm PES 18 cm2 48 180C4----------K500 ml 0.22 µm PES 39 cm2 12 180C5----------E1,000 ml 0.22 µm PES 62 cm2 12 180C6----------E

Water Throughput

old

84 Filtration Devices84 Chemical Compatibility

Material Minisart® Types

PES

mem

bran

e

SFCA

mem

bran

e

PTFE

mem

bran

e

RC m

embr

ane

Nyl

on m

embr

ane

GF

dept

h fi

lter

Hou

sing

MBS

Hou

sing

PP

Min

isar

t® H

ighF

low

Min

isar

t® N

ML

O

phth

alsa

rtM

inis

art®

NM

L Pl

us

Min

isar

t® N

ML

GF

Min

isar

t® H

Y M

inis

art®

Air

Min

isar

t® RC

Min

isar

t® N

Y

Min

isar

t® N

Y Pl

us

Min

isar

t® SR

P

Min

isar

t® PE

S

Filter Membrane PES SFCA PTFE RC PA PES SFCA SFCA PTFE RC PA PA PTFE PESPre-Filter GF - - GF GF - - - GF - -Housing Material MBS PP MBS MBS MBS MBS MBS PP PP PP PP PPSterilizationEthylene oxide ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++Gamma irradiation ++ ++ -1 ++ - ++ ++ - ++ ++ ++ ++ -1 - - - - -Autoclaving 121°C, 30 min ++ ++ ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ ++SolventsAcetone - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Acetonitrile - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Gasoline + ++ ++ ++ ++ ++ + ++ + + + + + ++ ++ ++ ++ +Benzene + + ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ +Benzyl alcohol + + ++ ++ ++ ++ - + - - - - - ++ ++ ++ ++ +n-Butyl acetate - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -n-Butanol ++ ++ ++ ++ ++ ++ + ++ + + + + + ++ ++ ++ ++ ++Cellosolve + - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ +Chloroform - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Cyclohexane - - ++ ++ ++ ++ + + - - - + + + + + + -Cyclohexanone - - ++ ++ ++ ++ - + - - - - - + + + + -

Diethylacetamide - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Diethyl ether - + ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Dimethyl formamide - - ++ + + ++ - ++ - - - - - + + + ++ -Dimethylsulfoxide - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Dioxane - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Ethanol, 98% ++ ++ ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ ++Ethyl acetate - - ++ ++ ++ ++ - + - - - - - + + + + -Ethylene glycol ++ + ++ ++ ++ ++ ++ ++ ++ + + ++ ++ ++ ++ ++ ++ ++Formamide ++ - ++ + ++ ++ ++ ++ ++ - - ++ ++ + ++ ++ ++ ++Glycerin ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++

n-Heptane + + ++ ++ ++ ++ ++ + + + + + ++ + + + + +n-Hexane + + ++ ++ ++ ++ ++ + + + + + ++ + + + + +Isobutanol ++ + ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ ++Isopropanol ++ ++ ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ ++Isopropyl acetate - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Methanol, 98% + - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ +Methyl acetate - - ++ ++ ++ ++ - + - - - - - + + + + -Methylene chloride - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Methyl ethyl ketone - + ++ ++ ++ ++ - + - - - - - + + + + -Methyl isobutyl ketone - - ++ ++ ++ ++ - + - - - - - + + + + -Monochlorobenzene + + ++ ++ ++ ++ - + - - - - - + + + + +Nitrobenzene - - ++ ++ + ++ - + - - - - - + + + + -n-Pentane ++ ++ ++ ++ ++ ++ + + + + + + + + + + + +Perchloroethylene - - ++ ++ ++ ++ - + - - - - - + + + + -

Chemical Compatibility

85Filtration DevicesChemical Compatibility

Material Minisart® Types

PES

mem

bran

e

SFCA

mem

bran

e

PTFE

mem

bran

e

RC m

embr

ane

Nyl

on m

embr

ane

GF

dept

h fi

lter

Hou

sing

MBS

Hou

sing

PP

Min

isar

t® H

ighF

low

Min

isar

t® N

ML

O

phth

alsa

rtM

inis

art®

NM

L Pl

us

Min

isart

® NM

L GF

Min

isar

t® H

Y M

inis

art®

Air

Min

isar

t® RC

Min

isar

t® N

Y

Min

isar

t® N

Y Pl

us

Min

isar

t® SR

P

Min

isar

t® PE

S

Filter Membrane PES SFCA PTFE RC PA PES SFCA SFCA PTFE RC PA PA PTFE PESPrefilter GF - - GF GF - - - GF - -Housing Material MBS PP MBS MBS MBS MBS MBS PP PP PP PP PPSolvents (continued)Pyridine - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Carbon tetrachloride - - ++ ++ ++ ++ - + - - - - - + + + + -Tetrahydrofuran - - ++ ++ ++ ++ - ++ - - - - - ++ ++ ++ ++ -Toluene - + ++ ++ ++ ++ - + - - - - - + + + + -Trichloroethane - - ++ ++ + ++ - + - - - - - + + + + -Trichloroethylene - + ++ ++ ++ ++ - + - - - - - + + + + -Xylene - + ++ ++ ++ ++ - + - - - - - + + + + -AcidsAcetic acid, 25% + + ++ ++ - ++ + ++ + + + + + ++ - - ++ +Acetic acid, 80% - - ++ + - ++ - + - - - - - + - - + -Hydrofluoric acid, 25% + - ++ + - ++ + + + - - + + + - - + +Hydrofluoric acid, 50% + - ++ + - ++ - + - - - - - + - - + +Perchloric acid, 25% - - ++ - - ++ - + - - - - - - - - + -Phosphoric acid, up to 10% + + ++ - - ++ + + + + + + + - - - + +

Phosphoric acid, 86% + + ++ - - ++ - + - - - - - - - - + +Nitric acid, 30% + - ++ - - ++ + + + - - + + - - - + +Nitric acid, conc. - - ++ - - ++ - - - - - - - - - - - -Hydrochloric acid, 15% ++ + ++ - - ++ + + + + + + + - - - + +Hydrochloric acid, 20% ++ - ++ - - ++ + + + - - + + - - - + +Sulfuric acid, 25% + - ++ + - ++ ++ ++ + - - ++ ++ + - - ++ +Sulfuric acid, 98% - - ++ - - ++ - - - - - - - - - - - -Trichloroacetic acid, 25% - - ++ ++ - ++ - + - - - - - + - - + -BasesAmmonia, 1N ++ + ++ + ++ ++ + ++ + + + + + + ++ ++ ++ ++

Ammonium hydroxide, 25% + + ++ + ++ + - + - - - - - + + + + +Potassium hydroxide, 32% ++ - ++ - + + - ++ - - - - - - + + ++ ++Sodium hydroxide, 1N ++ - ++ + ++ + - ++ - - - - - + ++ + ++ ++Sodium hydroxide, 32% ++ - ++ - + - - + - - - - - - + - + +Aqueous solutionsFormaldehyde, 30% + ++ ++ + ++ ++ + + + + + + + + + + + +Sodium hypochlorite, 5% ++ - ++ - - ++ + + + - - + + - - - + +Hydrogen peroxide, 35% ++ - ++ - - ++ + ++ + - - + + - - - ++ ++pH range pH 1 to 14 - - ++ - - ++ - ++pH 1 to 13 ++ - ++ - - ++ - ++pH 3 to 14 + - ++ + ++ ++ - ++pH 3 to 12 ++ - ++ ++ ++ ++ + ++pH 4 to 8 ++ ++ ++ ++ ++ ++ ++ ++

Contact time: 24 hours at 20°C. Chemical compatibilities can be influenced by various factors. Therefore, we recommend that you confirm compatibility with the liquid you want to filter by performing a trial filtration run before you start your actual filtration.

LegendHigh compatibility ++

Limited compatibility +

Not compatible -1 Gamma irradiation feasible for Minisart® Air

91 Filter Papers

95 Glass and Quartz Microfiber Filters

98 Membrane Filters

106 Blotting | Chromatography Papers and Blotting Membranes

108 Filtration Equipment

118 Combisart® Manifolds

Basic FiltrationTable of Contents

Basic FiltrationIntroduction 87

Basic Filtration88 Introduction

Filters are indispensable for your routine work in laboratory and industrial applications. Sartorius supplies you with a broad range of filters for a myriad of filtration tasks and supports you with all your filtration challenges.

Our Product Range Covers:– Filter papers– Glass and quartz microfiber filters– Extraction thimbles– Membrane filters– Blotting & chromatography papers & membranes– Filtration equipment

Quality Assurance and Quality ControlSartorius pays particular attention to continuous in-process quality control. Regular checks and exact analyses of the raw materials and each finished product assure constant high quality and product uniformity. We meet the requirements set forth by the ISO 9001 quality management system and the ISO 14001 environmental management system.

Basic Filtration 89Introduction

How Do Filter Papers Work?Filter papers are depth filters. Their efficiency is influenced by various parameters: the mechanical particulate retention, adsorption, pH, surface properties, thickness and strength of the filter paper as well as the shape, density and quantity of particles to be retained. The precipitates deposited on the filter form a “cake layer” which – depending on its density – increasingly affects the progress of an ongoing filtration and decisively affects the retention capability. Therefore, it is essential to select the perfect filter paper to ensure the best filtration results. This choice depends on the filtration method as well as on the amount and properties of the medium to be filtered, the size of the particulate solids to be removed and the required degree of clarification.

How Do Membrane Filters Work?Membrane filters retain particles larger than their pore sizes. Smaller particles pass through the membrane or are captured in the membrane. Such filters are used for the filtration of smaller particles and for critical applications such as sterility testing. The choice of the right membrane type depends on the specifications of the solution to be filtered. The most important parameters for this are adsorption, chemical compatibility and the particle size to be retained.

Basic Filtration90 Filter Papers

Ash-free Filter PapersFor Quantitative and Gravimetric Analyses

These filter papers are used for quantitative and gravimetric analyses as well as for pressure or vacuum filtration. They are made out of 100% cotton linters with an α-cellulose content of > 98% and are acid-washed to make the papers ashless and achieve high purity.

SpecificationsGrade Weight

(g/m²)Thickness (mm)

Particle Retention (µm)

Filtration (s)

Precipitates Properties

388 388 84 0.21 12 to 15 10 Coarse crystalline Wide-pore, loose structure, fast filtering

389 389 84 0.19 8 to 12 20 Medium-fine crystalline

Medium- to wide-pore, medium fast filtering

389 F 389 F 84 0.19 8 to 12 20 Medium-fine crystalline


392 392 84 0.17 5 to 8 50 Fine crystalline Medium dense, medium fast filtering

390 390 84 0.16 3 to 5 100 Fine crystalline Narrow-pore, dense, slow filtering

391 391 84 0.15 2 to 3 180 Very fine crystalline Fine-pore, dense, very slow filtering

393 393 100 0.17 1 to 2 300 Very fine crystalline Very fine-pore, very dense, very slow filtering


Filter Discs, 100 PiecesDia. in mm Grade 388 Grade 389 Grade 389 F Grade 390 Grade 391 Grade 392 Grade 39355 FT-3-101-055 FT-3-102-055 FT-3-112-055 FT-3-103-055 FT-3-104-055 FT-3-105-055 FT-3-127-05570 FT-3-101-070 FT-3-102-070 FT-3-103-070 FT-3-104-070 FT-3-105-070 FT-3-127-07090 FT-3-101-090 FT-3-102-090 FT-3-112-090 FT-3-103-090 FT-3-104-090 FT-3-105-090 FT-3-127-090110 FT-3-101-110 FT-3-102-110 FT-3-112-110 FT-3-103-110 FT-3-104-110 FT-3-105-110 FT-3-127-110125 FT-3-101-125 FT-3-102-125 FT-3-112-125 FT-3-103-125 FT-3-104-125 FT-3-105-125 FT-3-127-125150 FT-3-101-150 FT-3-102-150 FT-3-112-150 FT-3-103-150 FT-3-104-150 FT-3-105-150 FT-3-127-150185 FT-3-101-185 FT-3-102-185 FT-3-112-185 FT-3-103-185 FT-3-104-185 FT-3-105-185 FT-3-127-185240 FT-3-101-240 FT-3-102-240 FT-3-103-240 FT-3-104-240 FT-3-105-240 FT-3-127-240

Folded Filters, 100 PiecesDia. in mm Grade 388 Grade 389 Grade 389 F Grade 390 Grade 391 Grade 392110 FT-4-101-110 FT-4-102-110 FT-4-103-110 FT-4-104-110 FT-4-105-110125 FT-4-101-125 FT-4-102-125 FT-4-103-125 FT-4-104-125 FT-4-105-125150 FT-4-101-150 FT-4-102-150 FT-4-103-150 FT-4-104-150 FT-4-105-150185 FT-4-101-185 FT-4-102-185 FT-4-112-185 FT-4-103-185 FT-4-104-185 FT-4-105-185240 FT-4-101-240 FT-4-102-240 FT-4-104-240

Sheets in 580 + 580 mm, 100 PiecesGrade 388 Grade 389 Grade 390 Grade 391 Grade 392 Grade 393FT-2-101-580580 FT-2-102-580580 FT-2-103-580580 FT-2-104-580580 FT-2-105-580580 FT-2-127-580580

Other dimensions are available on request

Basic Filtration 91Filter Papers

Wet-strengthened Filter PapersFor Qualitative Analyses

These qualitative filter papers are essentially used for analytical purposes and routine analyses, whenever no gravimetric analyses are required. They are wet-strengthened and can be used for pressure and vacuum filtration. They are made of refined pulp and linters with an > 95% α-cellulose content and are very pure with an ash content ≤ 0.1%.




Filtration (s)

Precipitates Properties

1288 84 0.21 12 to 15 10 Coarse crystalline Wide-pore, loose structure, fast filtering

1289 84 0.21 8 to 12 20 Medium-fine crystalline


1292 84 0.17 5 to 8 50 Fine crystalline Medium dense, medium fast filtering

1290 84 0.15 3 to 5 100 Fine crystalline Narrow-pore, dense, slow filtering

1291 84 0.15 2 to 3 180 Very fine crystalline Fine-pore, dense, very slow filtering

293 80 0.15 1 to 2 300 Very fine crystalline Very fine-pore, very dense, very slow filtering


Filter Discs, 100 PiecesDia. in mm Grade 1288 Grade 1289 Grade 1290 Grade 1291 Grade 1292 Grade 29355 FT-3-206-055 FT-3-207-055 FT-3-208-055 FT-3-209-055 FT-3-210-055 FT-3-211-05570 FT-3-206-070 FT-3-207-070 FT-3-208-070 FT-3-209-070 FT-3-210-070 FT-3-211-07090 FT-3-206-090 FT-3-207-090 FT-3-208-090 FT-3-209-090 FT-3-210-090 FT-3-211-090110 FT-3-206-110 FT-3-207-110 FT-3-208-110 FT-3-209-110 FT-3-210-110 FT-3-211-110125 FT-3-206-125 FT-3-207-125 FT-3-208-125 FT-3-209-125 FT-3-210-125 FT-3-211-125150 FT-3-206-150 FT-3-207-150 FT-3-208-150 FT-3-209-150 FT-3-210-150 FT-3-211-150185 FT-3-206-185 FT-3-207-185 FT-3-208-185 FT-3-209-185 FT-3-210-185 FT-3-211-185240 FT-3-206-240 FT-3-207-240 FT-3-208-240 FT-3-209-240 FT-3-210-240

Folded Filters, 100 PiecesDia. in mm Grade 1288 Grade 1289 Grade 1290 Grade 1291 Grade 1292 Grade 293110 FT-4-206-110 FT-4-207-110 FT-4-208-110 FT-4-209-110 FT-4-210-110125 FT-4-206-125 FT-4-207-125 FT-4-208-125 FT-4-209-125 FT-4-210-125 FT-4-211-125150 FT-4-206-150 FT-4-207-150 FT-4-208-150 FT-4-209-150 FT-4-210-150 FT-4-211-150185 FT-4-206-185 FT-4-207-185 FT-4-208-185 FT-4-209-185 FT-4-210-185 FT-4-210-185240 FT-4-206-240 FT-4-207-240 FT-4-208-240 FT-4-209-240 FT-4-210-240 FT-4-211-240

Sheets in 580 + 580 mm, 100 PiecesGrade 1288 Grade 1289 Grade 1290 Grade 1291 Grade 1292 Grade 293FT-2-206-580580 FT-2-207-580580 FT-2-208-580580 FT-2-209-580580 FT-2-210-580580 FT-2-211-580580


Basic Filtration92 Filter Papers

High-Purity Filter PapersFor Qualitative Analyses




Filtration (s)

Material

292 87 0.18 5 to 8 45 Cotton linters, low-nitrogen and nitrates,ash content ≤ 0.06% according to DIN 54370

292a 97 0.19 4 to 7 60 Cotton linters, low-nitrogen and nitrates,ash content ≤ 0.06% according to DIN 54370

132 80 0.17 5 to 7 55 Cotton linters and refined pulp, low-phosphate and low-potassium, ash content < 0.02% according to DIN 54370

131 80 0.16 3 to 5 100 Cotton linters and refined pulp, low-phosphate and low-potassium, ash content < 0.02% according to DIN 54370


Filter Discs, 100 PiecesDia. in mm Grade 131 Grade 132 Grade 292 Grade 292a55 FT-3-351-055 FT-3-329-055 FT-3-205-055 FT-3-215-05570 FT-3-351-070 FT-3-329-070 FT-3-205-070 FT-3-215-07090 FT-3-351-090 FT-3-329-090 FT-3-205-090 FT-3-215-090110 FT-3-351-110 FT-3-329-110 FT-3-205-110 FT-3-215-110125 FT-3-351-125 FT-3-329-125 FT-3-205-125 FT-3-215-125150 FT-3-351-150 FT-3-329-150 FT-3-205-150 FT-3-215-150185 FT-3-351-185 FT-3-329-185 FT-3-205-185 FT-3-215-185240 FT-3-351-240 FT-3-329-240 FT-3-205-240 FT-3-215-240

Folded Filters, 100 PiecesDia. in mm Grade 131 Grade 132 Grade 292 Grade 292a110 FT-4-351-110 FT-4-329-110 FT-4-205-110 FT-4-215-110125 FT-4-351-125 FT-4-329-125 FT-4-205-125 FT-4-215-125150 FT-4-351-150 FT-4-329-150 FT-4-205-150 FT-4-215-150185 FT-4-351-185 FT-4-329-185 FT-4-205-185 FT-4-215-185

240 FT-4-351-240 FT-4-329-240 FT-4-205-240 FT-4-215-240

Sheets in 580 + 580 mm, 100 PiecesGrade 131 Grade 132 Grade 292 Grade 292aFT-2-351-580580 FT-2-329-580580 FT-2-205-580580 FT-2-215-580580


These paper grades are used for analytical purposes that require a low ash content. Grades 292 and 292a are especially suitable for soil analyses because they are low in nitrogen. For phosphate or sodium determination, we recommend grades 131 and 132.

Basic Filtration 93Filter Papers

Filter Papers For Qualitative-Technical Analyses

These filter papers are used for routine analyses like clarification, determination of substances, but also as discs with a center hole for technical applications. Grades with a wet burst resistance > 30 kPa are referred to as wet-strengthened and are therefore suitable for pressure or vacuum filtration. They are made of refined pulp and linters with an > 95% α-cellulose content, are very pure with an ash content between <0.1 to 0.15%. Below you will find an overview of the most commonly used grades.

SpecificationsGrade Surface Weight



Filtration (s)

Wet Burst Resistance(kPa)

Properties

3 hw Smooth 65 0.14 8 to 12 20 40 Medium fast filtering, filter paper for routine work in the lab

4 b Smooth 75 0.15 8 to 12 22 > 15 Medium fast filtering, filtration of coarse precipitates, wick paper for seed testing

603/N Crêped 75 0.25 > 15 8 ≥ 50 Fast filtering, filtration of sugar solutions6 Smooth 80 0.17 10 to 13 15 30 Fast filtering, degassing beer before

analysis, clarification of spirits100/N Smooth 85 0.18 6 to 8 30 80 Medium fast filtering, ash content < 0.1%,

low potassium and sodium content, determination of the sugar content

5 H/N Crêped 85 0.28 > 40 3 ≥ 40 Very fast filtering, wide-pore,filtration of essential oils

3 S/h Smooth 200 0.36 5 to 7 55 15 Medium fast to slow filtering, narrow-pore, re-wet test for diapers


Filter DiscsDia. in mm Grade 3 hw

(100 Pieces)Grade 4 b (100 Pieces)

Grade 603/N (100 Pieces)

Grade 6 (100 Pieces)

Grade 100/N (100 Pieces)

Grade 5 H/N(100 Pieces)

Grade 3 S/h(50 Pieces)

55 FT-3-303-055 FT-3-309-055 FT-3-312-055 FT-3-328-055 FT-3-307-05570 FT-3-303-070 FT-3-309-070 FT-3-312-070 FT-3-328-07090 FT-3-303-090 FT-3-309-090 FT-3-335-090 FT-3-312-090 FT-3-328-090 FT-3-423-090 FT-3-307-090110 FT-3-303-110 FT-3-309-110 FT-3-335-110 FT-3-312-110 FT-3-328-110 FT-3-307-110125 FT-3-303-125 FT-3-309-125 FT-3-335-125 FT-3-312-125 FT-3-328-125 FT-3-423-125 FT-3-307-125150 FT-3-303-150 FT-3-309-150 FT-3-335-150 FT-3-312-150 FT-3-328-150 FT-3-423-150 FT-3-307-150185 FT-3-303-185 FT-3-309-185 FT-3-335-185 FT-3-312-185 FT-3-328-185 FT-3-423-185 FT-3-307-185240 FT-3-303-240 FT-3-309-240 FT-3-335-240 FT-3-312-240 FT-3-328-240 FT-3-423-240 FT-3-307-240

Folded Filters, 100 PiecesDia. in mm Grade 3 hw Grade 4 b Grade 603/N Grade 6 Grade 100/N Grade 5 H/N125 FT-4-303-125 FT-4-309-125 FT-4-335-125 FT-4-312-125 FT-4-423-125150 FT-4-303-150 FT-4-309-150 FT-4-335-150 FT-4-312-150 FT-4-328-150 FT-4-423-150185 FT-4-303-185 FT-4-309-185 FT-4-335-185 FT-4-312-185 FT-4-423-185240 FT-4-303-240 FT-4-309-240 FT-4-335-240 FT-4-312-240 FT-4-328-240 FT-4-423-240270 FT-4-303-270 FT-4-309-270 FT-4-335-270 FT-4-312-270 FT-4-328-270 FT-4-423-270320 FT-4-303-320 FT-4-309-320 FT-4-335-320 FT-4-312-320 FT-4-328-320 FT-4-423-320

Sheets in 580 + 580 mm, 100 PiecesGrade 3 hw Grade 4 b Grade 603/N Grade 6 Grade 100/N Grade 5 H/NFT-2-303-580580 FT-2-309-580580 FT-2-335-580580 FT-2-312-580580 FT-2-328-580580 FT-2-423-580580


Basic Filtration94 Glass and Quartz Microfiber Filters

Glass Microfiber FiltersWithout Binder

Binder-free glass microfiber filters are recommended for analytical and gravimetric analyses and also as prefilters. These filters combine fast flow rates with high load capacity and the retention of very fine particles; they are biologically inert, are resistant to most chemicals and withstand temperatures up to 500°C (grade 550-HA up to 550°C).



Penetration 0.3 µm (%) *

Particle retention in liquids (µm)

Filtration speed (ml/min)

Fulfills the requirementsin EN 872:2005 (weigh loss)

MGA 55 0.25 < 0.002 1.6 510 YesMGB 140 0.70 < 0.002 1.0 210

MGC 52 0.26 < 0.002 1.2 335 YesMGD 120 0.53 < 0.1 2.7 920MGF 75 0.38 < 0.001 0.7 110

MGG 64 0.28 < 0.001 1.5 600

13440 88 0.44 0.7 120 YesMG 160 75 0.35 0.002 1.2 400MG 550-HA 65 0.27 1.5 400MG 169 68 0.33 1.0 130

* Measurement according to EN 143 (0.3 µm, 15 cm/s, paraffin oil)


Filter DiscsDia. in mm MGA

(100 Pieces)MG 160 (50 Pieces)

MGB (50 Pieces)

MGC (100 Pieces)

MGD (50 Pieces)

21 FT-3-1102-02125 FT-3-1101-025 FT-3-1102-025 FT-3-1103-025 FT-3-1104-02537 FT-3-1101-037 FT-3-01110-03747 FT-3-1101-047 FT-3-01110-047 FT-3-1102-047 FT-3-1103-047 FT-3-1104-04750 FT-3-1101-050 FT-3-01110-050 FT-3-1102-050 FT-3-1103-050 FT-3-1104-05055 FT-3-1101-055 FT-3-1102-055 FT-3-1103-05570 FT-3-1101-070 FT-3-01110-070 FT-3-1102-070 FT-3-1103-070 FT-3-1104-07080 FT-3-1101-08090 FT-3-1101-090 FT-3-01110-090 FT-3-1102-090 FT-3-1103-090 FT-3-1104-090100 FT-3-1101-100 FT-3-01110-100 FT-3-1102-100 FT-3-1103-100 FT-3-1104-100110 FT-3-1101-110 FT-3-01110-110 FT-3-1102-110 FT-3-1103-110 FT-3-1104-110125 FT-3-1101-125 FT-3-1102-125 FT-3-1103-125 FT-3-1104-125150 FT-3-1101-150 FT-3-1102-150 FT-3-1103-150 FT-3-1104-150

Basic Filtration 95Glass and Quartz Microfiber Filters

Dia. in mm MGF (100 Pieces)

MGG (100 Pieces)

MG 550-HA (100 Pieces)

13440*

24 FT-3-01147-02425 FT-3-1105-025 FT-3-1106-02542 13440--42------Q44 13440--44------Q47 FT-3-1105-047 FT-3-1106-047 FT-3-01147-047 13440--47------Q

50 FT-3-1105-050 FT-3-1106-050 FT-3-01147-050 13440--50------Q

55 FT-3-1105-055 FT-3-1106-055 FT-3-01147-05570 FT-3-1105-070 FT-3-1106-070 FT-3-01147-07090 FT-3-1105-090 FT-3-1106-090 FT-3-01147-090100 13440-100------K110 FT-3-1105-110 FT-3-1106-110 FT-3-01147-110125 FT-3-1105-125 FT-3-1106-125 FT-3-01147-125130 13440-130------K150 FT-3-1105-150 FT-3-1106-150

* Q = 500 pieces | K = 50 piecesOther dimensions as well as sheets are available on request

Basic Filtration96 Glass and Quartz Microfiber Filters

Glass Microfiber FiltersWith Binder

These filters are mostly used either for monitoring air and gas or as a prefilter. They are manufactured with synthetic binding agents to ensure that the filter has a defined strength. They are mechanically and chemically stable, have a temperature resistance up to 180°C and – depending on the binding agent used – are either hydrophobic or hydrophilic.


(g/m2)Thickness (mm)

Penetration 0.3 µm (%)*

Pressure drop5.3 cm/s (Pa)

Binding agent

MG 227/1/60 60 0.32 < 0.5 260 Hydrophobic13430 220 1.25 0.02 360 Hydrophilic

13400 73 0.39 0.015 363 HydrophilicMG 227 75 0.40 < 0.01 350 HydrophobicMG 161 75 0.40 ≤ 0.002 ≤ 580 Hydrophilic

MG 400 80 0.38 < 0.001 400 HydrophilicMG 1387/1 90 0.37 ≤ 0.003 400 Hydrophilic

* Tested and classified according to the Standard EN 143


Filter DiscsDia. in mm

MG 221/1/60 (100 Pieces)

13430** 13400** MG 227 (100 Pieces)

MG 1387/1(50 Pieces)

13 13400--13------S20 13400--20------S25 13400--25------Q42 13400--42------Q44 13400--44------Q45 13400--45------Q FT-3-01125-04547 13430--47------S 13400--47------Q FT-3-01120-047 FT-3-01125-04750 13400--50------Q FT-3-01125-05055 FT-3-01120-055 FT-3-01125-05580 13400--80------N100 13430-100------K 13400-100------K110 FT-3-01120-110 FT-3-01125-110120 13400-120------K124 13430-124------K 13400-124------K125 FT-3-01125-125127 13430-127------K 13400-127------K130 13430-130------K 13400-130------K FT-3-01125-130150 FT-3-01124-150

** K = 50 pieces, N = 100 pieces, Q = 500 pieces, S = 200 piecesOther dimensions as well as sheets are available on request

Basic Filtration 97Glass and Quartz Microfiber Filters

Quartz Microfiber Filters

SpecificationsGrade Material Weight


Penetration,0.3 µm 15 cm/s*

Temperature Resistance

Q3400 100% Quartz microfiber silicium dioxide (SiO2)

85 0.43 < 0.002 up to 900°C

* Tested and classified according to the Standard EN 143


Filter DiscsDia. in mm Q340020 Q3400--20------G25 Q3400--25------G37 Q3400--37------G45 Q3400--45------G 47 Q3400--47------G50 Q3400--50------G

82 Q3400--82------N90 Q3400--90------N142 Q3400-142------K150 Q3400-150------K

* G = 25 pieces, K = 50 pieces, N = 100 piecesOther dimensions as well as sheets are available on request

The quartz microfiber material of the Sartorius pre-heated filters, grade Q3400, is made of high-purity quartz microfiberswithout any addition of glass microfibers or binding agents. In addition, the Q3400 filter grade is tempered to remove allchemically combined water and to give the filters excellent weight and dimensional stability. Sartorius filters are especiallysuitable for emissions monitoring at temperatures of up to 900°C and wherever filters of the highest purity are needed.

Basic Filtration98 Membrane Filters

Membrane Filtration – Quick Selection Guide

RC Regenerated Cellulose – Particle Removal

PA Polyamide | Nylon – Particle Removal

PTFE Polytetra-fluoroethylene – Particle Removal

Highly Particle-laden SolutionsPrefiltration

Depth filters (glass microfiber filters | filter papers)

Membranes, pore-sizes 8 – 0.8 µm (→ CN, CA)

Solvents Aqueous Air

Solvent-Mixtures

PES Polythersulfone – Pharmaceutical Solutions

RC Regenerated Cellulose – Particle Removal, De-gassing

PA Polyamide|Nylon – Particle Removal, Alkaline Solutions

PC-TEM Polycarbonate Track-etched Membrane – Particulate Analysis

PTFE Polytetra-fluoroethylene – Particle Removal

CN Cellulose Nitrate – Particle Retention and Cell Capture

CA Cellulose Acetate – Particle Retention and -Removal

RC Regenerated Cellulose – General Purpose Filtration

PES Polyethersulfone – Cell Culture Media, Buffers

PC-TEM Polycarbonate Track-etched

Membrane – Particulate- and Cell Analysis

polar = hydrophilic

non-polar = hydrophobic hydrophobic membranes need to be pre-wetted with ethanol or methanol before the filtration of aqueous solutions

PTFE Polytetra-fluoroethylene – Sterile Venting

CN Cellulose Nitrate – Particle- and Cell Retention

CA Cellulose Acetate – Particle Retention

PC-TEM Polycarbonate Track-etched

Membrane – Air Monitoring

Sample

Basic Filtration 99Membrane Filters

Cellulose nitrate membrane filters are indicated for many general laboratory applications where a membrane with a high non-specific adsorption is suitable. They are hydrophilic, have high flow rates thanks to their symmetric structure and are compatible with aqueous solutions

(pH 4 to 8), hydrocarbons and several other organic solvents. The cellulose nitrate membranes are available in different pore sizes from 0.2 µm to 8 µm.

Cellulose Nitrate

SpecificationsType Pore Size

(µm)Thickness (µm)

Bubble Point (bar)

Water Flow Rate (ml/min/cm2/bar)

Burst Pressure (bar)

11327 0.2 130 4.2 25 > 0.3511306 0.45 130 2.4 70 > 0.311305 0.65 130 2 130 > 0.2511304 0.8 130 1.4 200 > 0.211303 1.2 130 1 200 > 0.211302 3 130 0.5 430 > 0.211342 5 130 0.5 570 > 0.1511301 8 130 0.3 750 > 0.1


Filter DiscsDia. in mm 11301 (8 µm)* 11302 (3 µm)* 11303 (1.2 µm)* 11304 (0.8 µm)*13 11301--13------N 11302--13------N 11303--13------N 11304--13------N20 11304--20------N25 11301--25------N 11302--25------N 11303--25------N 11304--25------N30 11304--30------N37 11301--37------N 11304--37------N47 11301--47------N 11302--47------N 11303--47------N 11304--47------N

50 11301--50------N 11302--50------N 11303--50------N 11304--50------N68 11302--68------G70 11301--70------G90 11302--90------G 11303--90------G 11304--90------G100 11301-100------N 11302-100------G 11303-100------G 11304-100------G

Dia. in mm 11305 (0.65 µm)* 11306 (0.45 µm)* 11327 (0.2 µm)* 11342 (5 µm)*13 11305--13------N 11306--13------N 11327--13------N 11342--13------N20 11306--20------N25 11305--25------N 11306--25------N 11327--25------N 11342--25------N30 11306--30------N37 11306--37------N47 11305--47------N 11306--47------N 11327--47------N 11342--47------N

50 11305--50------N 11306--50------N 11342--50------N55 11306--55------N85 11306--85------N90 11306--90------N 11342--90------G100 11305-100------N 11306-100------N 11342-100------G110 11306-110------N

* G = 25 pieces, N = 100 pieces Other dimensions and packaging units are available on request


Cellulose Acetate



Bubble Point (bar)



11107 0.2 120 2.9 24 0.811106 0.45 120 1.9 69 0.711105 0.65 120 1.5 115 0.711104 0.8 120 1 200 0.512303 1.2 140 0.8 320 0.412342 5 140 0.4 570 0.25


Filter DiscsDia. in mm

11104 (0.8 µm)*

11105 (0.65 µm)*

11106 (0.45 µm)*

11107 (0.2 µm)*

12303 (1.2 µm)*

12342 (5 µm)*

13 11104--13------N 11106--13------N 11107--13------N25 11104--25------N 11105--25------N 11106--25------N 11107--25------N 12303--25------N 12342--25------N30 11106--30------N 11107--30------N37 11106--37------N4547 11104--47------N 11105--47------N 11106--47------N 11107--47------N 12303--47------N 12342--47------N50 11104--50------N 11105--50------N 11106--50------N 11107--50------N 12303--50------N

7085 11106--85------N90 11104--90------N 11105--90------G 11106--90------G 11107--90------G100 11106-100------N 11107-100------N 12303-100------G110 11106-110------N

* G = 25 pieces, N = 100 piecesOther dimensions and packaging units are available on request

Cellulose acetate membranes combine high flow rates and thermal stability with very low adsorption characteristics, and are therefore excellently suited for use in pressure filtration devices. They are hydrophilic, have high flow rates thanks to their symmetric structure and are compatible with aqueous solutions

(pH 4-8), oils, alcohols and other organic solvents. The 0.2 µm membrane is the filter of choice for sterile filtration of aqueous solutions, such as nutrient media, buffers and sera. The cellulose acetate membranes are available in different pore sizes from 0.2 to 5 µm.


The very low adsorption membranes are hydrophilic, solvent-resistant (pH 3-12) and therefore suited for the particle removal from solvents. The membrane is asymmetric

and reinforced with nonwoven cellulose. They are available in two pore sizes: 0.45 µm and 0.2 µm.

Regenerated Cellulose



Bubble Point (bar)


18407 0.2 170 4.4 1518406 0.45 170 2.9 30


Filter DiscsDia. in mm 18406 (0.45 µm)* 18407 (0.2 µm)*13 18406--13------N 18407--13------N25 18406--25------N 18407--25------N47 18406--47------N 18407--47------N50 18406--50------N 18407--50------N

100 18406-100------G 18407-100------G



Polyethersulfone



Bubble Point (bar)



15458 0.1 150 3.8 10 > 0.6

15407MI 0.2 150 3.5 25 > 0.515406 0.45 150 2.6 46 > 0.5


Filter DiscsDia. in mm 15406 (0.45 µm)* 15407MI (0.2 µm)* 15458 (0.1 µm)*25 15406--25------N 15407--25----MIN 15458--25------N

47 15406--47------N 15407--47----MIN 15458--47------N50 15406--50------N 15407--50----MIN 15458--50------N90 15407--90----MIK

*K = 50 pieces, N = 100 piecesOther dimensions are available on request

Polyethersulfone (PES) membrane filters are hydrophilic, have high flow rates thanks to their symmetric structure, have a low non-specific protein adsorption and are chemically resistant over a pH range of

1 – 14. They are therefore recommended for the filtration of aqueous solutions as well for protein filtration. Furthermore, the low level of extractables makes them suitable for environmental analysis.


Polyamide membrane filters are hydrophilic and chemically resistant to alkaline solutions and organic solvents. They are therefore recommended for the particle removal from aqueous solutions and

solvents for analytical determination such as HPLC, for the sterile filtration of these liquids as well as for applications where a membrane with a relatively high non-specific adsorption is suitable.

Polyamide



Bubble Point (bar)

Water Flow Rate(ml/min/cm2/bar)


25007 0.2 115 3.2 15 > 0.2525006 0.45 115 2.3 35 > 0.23


Filter DiscsDia. in mm 25006 (0.45 µm)* 25007 (0.2 µm)*13 25006--13------N 25007--13------N

25 25006--25------N 25007--25------N47 25006--47------N 25007--47------N50 25006--50------N 25007--50------N55 25007--55------N90 25006--90------G 25007--90------G



Hydrophobic PTFE

The main application of these membrane filters is the filtration of air, gases or chemicals. They are made of PTFE (polytetra-fluorethylene) only and are therefore permanently hydrophobic. Unlike other (hydrophilic) filter types, they are not wetted by air humidity, allowing unhindered passage of air at low differential pressures as well. PTFE

membrane filters have an excellent chemical compatibility (pH 1 to 14), so that they are also used for the filtration of solvents and acids, to which other filter types are not resistant. Due to their hydrophobic characteristics, they must be pre-wetted with ethanol or methanol before the filtration of aqueous media.



Bubble Point (bar)

Isopropanol Flow Rate(ml/min/cm2/bar)

11807 0.2 65 1.4 1111806 0.45 80 0.9 2011803 1.2 100 0.45 8011842 5 100 0.10 250


Filter DiscsDia. in mm 11803 (1.2 µm)* 11806 (0.45 µm)* 11807 (0.2 µm)* 11842 (5 µm)*13 11803--13------N 11806--13------N 11807--13------N

25 11803--25------N 11806--25------N 11807--25------N 11842--25------N37 11806--37------N42 11842--42------N

47 11803--47------N 11806--47------N 11807--47------N 11842--47------N

50 11803--50------N 11806--50------N 11807--50------N 11842--50------N

90 11803--90------G 11806--90------G 11807--90------G

100 11803-100------G 11806-100------G 11807-100------G 11842-100------G

* G= 25 pieces, N= 100 piecesOther dimensions and packaging units are available on request

Basic Filtration 105

Those white and hydrophilic polycarbonate track-etched membranes are manufactured from high grade polycarbonate film using track-etch technology. Their capillary pore structure is uniform and precise, with a narrow pore size distribution to retain particles on their surface. Track-etched membranes are an excellent choice for accurate fractionation of particulates because of their precise pore size.

Track-etch technology offers the user distinct performance advantages when excellent surface capture and high sample visibility are required. Their main applications are particulate analysis, epifluorescence microscopy, fluid clarification, cytology, cell biology, bioassays, water microbiology and environmental analysis.

Membrane Filters



Bubble Point (bar)

Water Flow Rate(ml/min/cm2/0.7 bar)


23007 0.2 25 4.8 > 10 > 0.723006 0.4 25 2.5 > 30 > 0.7


Filter Discs, 100 PiecesDia. in mm 23006 (0.4 µm)* 23007 (0.2 µm)*25 23006--25------N 23007--25------N47 23006--47------N 23007--47------N50 23007--50------N


Polycarbonate Track-Etched

Basic Filtration106 Blotting | Chromatography Papers

Blotting | Chromatography Papers

These papers are made of cotton linters only with α-cellulose content of > 98%. These highly pure papers are not only ideal for blotting and chromatography, but also for a



Capillary Rise (mm/30 min)

Capillary Rise (mm/10 min)

Properties

FN 4 125 0.24 95 Chromatography paper, ash content < 0.04%FN 7 150 0.32 145 Chromatography paper, ash content < 0.04%FN 30 320 0.90 240 Chromatography paper, ash content < 0.04%,

paper for antibiotic test stripsFN 100 195 0.35 115 70 The most commonly used chromatography and

blotting paperBF 3 330 0.76 30 130 Blotting paper to increase and maintain the

transport of liquids


Sheets in 580 + 600 mmGrade FN 4(100 Sheets)

Grade FN 7(50 Sheets)



Grade BF 3(50 Sheets)

FT-2-504-580600N FT-2-507-580600K FT-2-526-580600G FT-2-527-580600K FT-2-520-580600K

Other dimensions and shapes are available on request

wide range of absorption applications like those common in the life sciences and diagnostics. Below you will find an overview of the most commonly used grades.

Basic Filtration 107Blotting | Chromatography Papers

Specifications

0.22 µm 0.45 µmMaterial Cellulose nitrate Cellulose nitrate

Thickness 120 µm 130 µm

Water flow rate 27 ml/(min.cm2 bar) 70 ml/(min.cm2 bar)

Bubble point 4.4 bar 2.4 bar

Wettability in water < 1 s < 1 s

Extractable content in water < 1% < 1%

Burst pressure 0.8 bar 0.2 bar

Binding capacity for IgG 200 µg/cm2 200 µg/cm2


Roll Size Order No.NC 0.22 µm 30 cm + 3 m 11327------41BL

NC 0.45 µm 30 cm + 3 m 11306------41BL

All indicated data to be understood as typical average values

Nitrocellulose Membrane for Blotting

Sartorius nitrocellulose membranes are available in two pore sizes, 0.22 µm and 0.45 µm. Both versions combine the advantages of high protein binding capacity with low background and high membrane stability, which ensures easy handling. Due to its large surface area, the 0.22 µm

membrane version is recommended for small proteins. Sartorius blotting membranes are ideal for western blotting, DNA blotting as well as dot or slot blots. They have been optimized for all protein blotting systems, such as electrotransfer, semi-dry or simple capillary blotting.

Basic Filtration108 Filtration Equipment

Re-usable, 13 mm Syringe Filter HoldersFor the Ultracleaning of Small Volumes Up to About 10 ml

SpecificationsConnectors Female Luer Lock inlet, luer slip outletChemical compatibility As for polycarbonate and siliconeFiltration area 0.5 cm2

Materials Polycarbonate top and bottom part,silicone gasket

Max. operating pressure 7 bar | 700 kPa | 101.5 psiMembrane filter diameter 13 mmSterilization By autoclaving at 121°CHold-up volume Less than 0.2 ml after overcoming the

bubble point (0.3 ml before)


Description Order No. 13 mm Polycarbonate Syringe Filter Holder, pack of 12 16514----------E

Silicon gasket, 10 + 14.9 + 0.5 mm, pack of 10 6980569

PTFE Holder for Solvents and Chemicals Made completely of PTFE, this holder is unaffected by chemicals and contains no trace elements which could be released into the liquid being filtered. It is therefore extremely well suited for particle removal from samples and reagents for analytical methods, such as NMR samples. Other

benefits of this application are the low hold-up volume, the easy cleaning and the drying at a temperature of 180°C. The construction of the holder ensures leak proof sealing without a sealing ring, and avoids twisting of the membrane filter when the top is tightened onto the base.

SpecificationsConnectors Female Luer Lock inlet, luer slip outletChemical compatibility As for PTFEFiltration area 0.5 cm2

Materials PTFE top and bottom partsMax. operating pressure 5 bar | 500 kPa | 72.5 psiMembrane filter diameter 13 mmSterilization By autoclaving (max. 134°C)

or by dry heat (max. 180°C)Hold-up volume Less than 0.03 ml after overcoming the



Description Order No.13 mm PTFE Syringe Filter Holder 16574

Polycarbonate Holder for Aqueous Solutions This inexpensive filter holder is made of clear, autoclavable polycarbonate. The silicone gasket enables a leak-free

filtration at pressures of up to 7 bar by simply screwing it together manually. Filter supports in the top and bottom parts allow filtration in either direction.

Basic Filtration 109Filtration Equipment

Re-usable 25 mm Syringe Filter HoldersFor the Ultracleaning and Sterilizing Filtration of Volumes of Up to About 100 ml

Stainless Steel Holder for Solvents and Chemicals Made of stainless steel, this holder is heat-resistant, and the chemical compatibility depends only on the inserted filter type.

SpecificationsConnectors Female Luer Lock inlet, luer slip outletChemical compatibility As for stainless steelFiltration area 3 cm2

Materials Stainless steel (1.4305) top and bottom partsMax. operating pressure 7 bar | 700 kPa | 101.5 psiMembrane filter diameter 25 mmSterilization By autoclaving (max. 134°C)

or by dry heat (max. 180°C)Hold-up volume Less than 0.1 ml after overcoming the



Description Order No.25 mm Stainless Steel Holder 16214Tightening tool, Polyman 24/5 6980595

Polycarbonate Holder for Aqueous Solutions This inexpensive filter holder is made of clear, autoclavable polycarbonate. The silicone gasket enables a leak-free filtration

SpecificationsConnectors Female Luer Lock inlet, luer slip outletChemical compatibility As for polycarbonate and siliconeFiltration area 3 cm2

Materials Polycarbonate top and bottom parts, silicone gasket

Max. operating pressure 7 bar | 700 kPa | 101.5 psiMembrane filter diameter 25 mmSterilization By autoclaving at 121°CHold-up volume Less than 0.3 ml after overcoming the



Description Order No.25 mm Polycarbonate Syringe Filter Holder, pack of 12 16517----------E

Silicone gasket, 20.5 + 26.5 + 0.5 mm, pack of 10 6980570

The top part can easily be mounted on the bottom part using the enclosed tightening tool. Filter supports in the top and bottom parts allow filtration in either direction.

at pressures of up to 7 bar by simply screwing it together manually. Filter supports in the top and bottom parts allow filtration in either direction.


25 mm Glass Vacuum Filter HolderFor Hybridization Tests, Particle Testing and Clarification

This filter holder is available in two versions differing from each other only in the type of the filter support. The filter withglass frit ensures uniform distribution of retained particles and is therefore recommended when the residue on the filter surface is of interest. Because it is easy to clean, the device with the PTFE-coated screen support is preferable when the filtrate is required, or when liquids difficult to remove from glass frits must be examined. The PTFE ring, which holds the

SpecificationsOutlet spout 12 mm dia.Parts and materials Borosilicate glass funnel and base

PTFE | glass filter support (type 16306) orPTFE | stainless steel filter support, coated withPTFE (type 16315)Silicone O-ring 25 + 3 mmAnodized Aluminium clamp

Chemical compatibility As for glass, PTFE and silicone.The silicone O-ring can be replaced by a fluoroelastomer O-ring (order no. 00118)

Funnel capacity 30 mlFiltration area 3 cm2

Max. operating pressure Only for vacuumSuitable membrane filter diameter 25 mm (or 24 mm)Sterilization By autoclaving (max. 134°C) or

by dry heat (max. 180°C)

6980115 glas funnel 30 ml

6983008 small clamp

17149 PTFE ring with PTFE-coated underdrain screen and support screen including a silicone O-ring

17148 PTFE ring with glass frit and a silicone O-ring 6980117

silicone O-ring

6980116 glass base


Description Order No.

Glass vacuum filtration holder for 25 mm (or 24 mm) membrane filter, with glass frit filter support

16306

Glass vacuum filtration holder for 25 mm (or 24 mm) membrane filter, with PTFE-coated screen filter support

16315

Replacement parts are shown in the diagram.

glass frit and the screen support, allows for the autoclaving of the devices with a filter in position and protects the edge of the glass frit from breakage and potential leakage. It has a rim around the upper edge to simplify the positioning of the membrane filter when inserted and a silicone O-ring in the underside for a leak-proof seal on the filtrate side. The funnel-shaped top part simplifies filling in the sample.


This filter holder is available in two versions differing from each other only in the type of the filter support. The device with glass frit ensures uniform distribution of retained particles and is therefore recommended, when the residue on the filter surface is of interest. Because it is easy to clean, the device with the PTFE-coated screen support is preferable when the filtrate is required, or when liquids difficult to remove from

SpecificationsOutlet spouts 15 mm dia.Parts and materials Borosilicate glass funnel and base

Silicone caoutchouc lidPTFE | glass filter support (type 16307) or PTFE | stainless steel filter support, coated with PTFE (type 16316)Silicone O-ring 45 + 3 mmAnodized Aluminium clamp

Chemical compatibility As for glass, PTFE and silicone.The silicone O-ring can be replaced by a fluoroelastomer O-ring (order no. 00124).

Funnel capacity 250 mlFiltration area 12.5 cm2

Max. operating pressure Only for vacuumSuitable membrane filter diameter 50 mm (or 47 mm)Sterilization By autoclaving (max. 134°C) or



Description Order No.Glass vacuum filtration holder for 50 mm (or 47 mm) membrane filter, with glass frit filter support

16307

Glass vacuum filtration holder for 50 mm (or 47 mm) membrane filter, with PTFE-coated screen filter support

16316


50 mm Glass Vacuum Filter HolderFor Particle Testing or Clarification and Sterile Filtration

6983007

6980121 lid

6980119 glass funnel, 250 ml

6983009 large clamp

6980120 glass base, 50 mm

17146 PTFE ring with glass frit and a silicone O-ring

6983006 glass frit

17147 PTFE ring with PTFE-coated underdrain screen and support screen including a silicone O-ring

6983010 drain screen

6980123 silicone O-ring

6983007 PTFE- coated support screen

glass frits must be examined. The PTFE ring, which holds the glass frit and the screen support, allows the autoclaving of the devices with a filter in position and protects the edge of the glass frit from breakage and potential leakage. It has a rim around the upper edge to simplify the positioning of the membrane filter when inserted and a silicone O-ring in the underside for a leak-proof seal on the filtrate side.



Description Order No.All-glass vacuum filter holder for 50 mm (or 47 mm) membrane filter, with vacuum-resistant flask, capacity 1 liter

16309


All-Glass Vacuum Filter HolderFor Analytical Determinations, Particle Removal from Solvents

All areas, where liquid and device can come into direct contact, are made of glass or PTFE. The device, in combination with solvent-resistant, hydrophilic RC-membranes, is therefore ideal for ultracleaning and degassing solvents and solvent mixtures for HPLC, GC and AA. Convenience of handling is ensured by several beneficial features. A 6 mm wide

SpecificationsParts and materials Borosilicate glass funnel, base and flask,

sintered glass frit in a PTFE ring and fluoroelastomer O-ring (45 + 3 mm) underneath, anodized aluminium clamp

Chemical compatibility As for glass and PTFEFunnel capacity 250 mlCapacity of the filtrate flask 1 literFiltration area 12.5 cm2

Max. operating pressure Only for vacuum

Suitable membrane filter diameter 50 mm (or 47 mm), 40 or 42 mm prefilter

Sterilization (without connector) By autoclaving (max. 134°C) orby dry heat (max. 180°C)

6980119 glass funnel

6983004 complete filter support

6983001 glass flask

6983002 glass base

6983005 fluoroelastomer gasket for connector

6983003 Polypropylene connector (with gasket)

00124 fluoroelastomer O-ring (for the filter support)

6983009 large clamp

non-ground rim above the ground glass neck of the suction flask prevents the filtrate from contacting grease on the ground glass surface and so avoids its contamination while being poured out of the flask. The hose nipple connector is made of polypropylene for safe connection of the vacuum hose. The filtrate outlet spout ends well below the entrance to this hose nipple.


This holder is made of stable, autoclavable polycarbonate. This practical holder is suitable for many simple laboratory filtrations. It can be connected to a peristaltic pump or a pressure container. The bell-shaped base protects the filtrate from repeated contamination while flowing in a receiver. The holder is characterized

SpecificationsChemical compatibility As for polycarbonate, polypropylene and siliconeFiltration area 12.5 cm2

Weight 83 gThreads for connectors M 12 + 1 female threadMaterials Polycarbonate top part, base part and hose

nipple, polypropylene filter support, silicone O-ring (40 + 5 mm)

Max. operating pressure 7 bar | 700 kPa | 101.5 psiSuitable membrane filter diameter 50 mm (40 or 42 mm prefilter)Sterilization By autoclaving at 121°C

The material withstands repeated cycles, provided aggressive cleaning agents are completely washed off and that the boiler water does not contain anti-corrosive or anti-scaling additives.


Description Order No.Polycarbonate in-line filter holder for 50 mm membrane filter,pack of 5.

16508----------B


Polycarbonate In-Line Filter HolderFor the Filtration of Liter Volumes of Aqueous Solutions

by an excellent resistance to pressure and density setting by simple hand-tightening. The transparent top part allows the visual control of the correct fit of the O-ring. The hose nipples can be replaced by luer connectors to use it as a large area syringe filter holder.

6985004 connector

00423upper part

6980232 filter support

6980110 silicone O-ring

6980232 filter support

6980383 base part

6985004 connector


25 mm Stainless Steel Filter HolderFor In-Line Filtration

The G¼ connection threads with density barrel, guarantee leak-proof sealing of the hose nipple and the holder without sealing rings. Other connectors, available as accessories, fit the holder onto reducing

SpecificationsConnectors Hose nipples DN10Filtration area 3 cm2

Weight ca. 170 gMaterials Stainless steel, except silicone

O-ring (21 + 2 mm) and aluminium closing ringMax. operating pressure 5 bar | 500 kPa | 72.5 psi

Suitable membrane filter 25 mm (20 mm prefilter for the filtration of liquids only)

Sterilization By autoclaving (max. 134°C) orby dry heat (max. 180°C)



Stainless steel pressure filter holder for 25 mm dia. membrane filter.

16251


6980176 Connector

6981031 Top part

6981033 Silicone O-ring

6981032 Base part

6981034 Closing ring

6980176 Connector

00177Ga male thread

01029Ga female thread

01030G4 female thread

valves or pumps with G¼ female thread (order no. 01030) or Ga female thread order no. 01029) or onto pressure tanks with Ga male thread (order no. 00177).


The filter holder is suitable for a pressure of up to 20 bar. The inlet side valve is convenient for the intermittent run-off of waste water. Other connectors, available as accessories, fit the holder onto reducing

SpecificationsConnectors Hose nipples DN10Connection thread M12 + 1Filtration area 13 cm2

Weight ca. 490 gMaterials Stainless steel, except silicone O-ring

(42 + 3 mm), PTFE and fluoroelastomer valve seals

Max. operating pressure 20 bar | 2,000 kPa | 290 psiSuitable membrane filter 47 mm (40 or 42 mm prefilter)Sterilization By autoclaving (max. 134°C) or



Description Order No.Stainless steel filter holder for 47 mm membrane filter (with adapter M12 + 1 male thread to hose barb DN10, Mat. 316, ref. 6980801) – Replacement parts are shown in the diagram

16254

Stainless steel filter holder for 47 mm membrane filter (with adapter M12 + 1 male thread to hose barb DN 4 to 5, Mat. 316, ref. 6981132)

16278

Stainless steel back pressure screen 6980721-----1Stainless steel filter support screen 6980180-----1Stainless steel underdrain screen 00181

Stainless steel connector M12 + 1 male thread to hose barb DN 4-5

6981132

Adapter Quick connect nipple length 60 mm male part to male thread M12 + 1, Mat 316

17090-----1

47 mm Stainless Steel Filter HolderFor In-Line Filtration

6982005 closing ring

6982003 top part

6980721 PTFE-coated back pressure screen6980178 silicone O-ring, 42 + 3 mm6980180 PTFE-coated filter support screen6980737 PTFE-coated under-drain screen

6982006 base plate

6980801 Stainless steel nipple for 10 mm hose

6980722 Vent valve

6980656 PTFE flat gasket

6980717 fluoroelasto-mer O-ring, 3 + 1.5 mm

6980801Stainless Steel nipple for 10 mm hose

valves or pumps with Ga female thread (order no. 17089) or onto pressure tanks with Ga male thread (order no. 17069) or on taps with G3 male thread (order no. 17068).

Diagram for 16254


A practical filter holder for many laboratory filtrations. It can be attached to a tripod with the help of a steel rod which can be screwed in. The hose nipple is screwed into the side of the top part, leaving room for a large filling opening. This makes pouring in the sample easier, and the sample can be refilled without removing the tube connection to the pressure source. Leak-proof sealing is achieved by

SpecificationsChemical compatibility As for stainless steel, PTFE and silicone.

If required, the silicone O-ring in the filtersupport can be replaced by a fluoroelastomer O-ring 00179 or a PTFE O-ring 17038 (by reducing the max. operating pressure to 4 bar | 58 psi); the silicone O-ring in the top part can be replaced by a fluoroelastomer O-ring 17145.

Filtration area 13 cm2

Weight 960 g

Threads for connectors M 12 + 1 female thread

Materials Top part, barrel, base part, corrugated iron,closing ring, closure cap, back pressure screenand stainless steel hose nipples 1.4401 (AISI316), PTFE-coated stainless steel filter support,silicone O-rings, 41 + 2 mm (top part) and42 + 3 mm (filter support), PTFE-sealing (cap).

Max. operating pressure 10 bar | 1,000 kPa | 145 psi

Suitable membrane filter diameter 47 mm (40 or 42 mm prefilter)

Sterilization By autoclaving (max 134°C) or by dry heat (180°C)



Stainless steel pressure filter holder 16249

Replacement Parts


Fluoroelastomer O-ring, 42 + 3 mm 00179

PTFE O-ring, 42 + 3 mm 17038

Fluoroelastomer O-ring for upper part, 41 + 2 mm 17145

Other replacement parts are shown in the diagram or on page 107.

Stainless Steel Pressure Filter HolderFor the Filtration of Up to 5 Liter Volumes

6980151 Filling port closure, com-plete with PTFE stopper6986055

PTFE stopper for 6980151

6986084 top part

6982020 silicone O-ring, 41 + 2 mm

6982005 closing ring

6986083 barrel (200 ml)

6980178 silicone O-ring, 42 + 3 mm6980737 PTFE-coated underdrain screen6982006 base part

6981288 Steel rod

6980801 Hose nipple

6980180 PTFE-coated filter support screen

6980801Hose nipple

hand-tightening the closing ring. For the filtration of small volumes (up to about 200 ml of soil samples or viscous liquids, such as oils), the holder is connected directly to a pressure source. For the filtration of up to 5 liter volumes of relatively easily filterable liquids (e.g. buffer solutions, solutions for cell counters and tissue culture solutions), it is used in combination with a pressure tank.


Chemical-resistant PTFE Filter HolderFor the Filtration of Aggressive Liquids

The holder hinders the release of trace elements into the filtrate and is resistant to almost all chemicals. The fluoroelastomer O-ring in the top part allows easy hand tightening, and can be replaced by a PTFE

SpecificationsChemical compatibility As for PTFE and fluoroelastomer

Filtration area 12.5 cm2

Thread for inlet connector M 14 + 1.5 male thread

Materials Top part, barrel, base part: corrugated iron,hose nipples and filter support with 40 + 3.5 mm O-ring: PTFE,locking rings: aluminium39 + 3.5 mm fluoroelastomer O-ring (top part)

Max. operating pressure 5 bar | 500 kPa | 72.5 psi

Suitable membrane filter diameter 47 mm

Sterilization By autoclaving (max 134°C) orby dry heat (180°C)



PTFE pressure filter holder, 47 mm, with 200 ml capacity. 16579

Replacement Parts


PTFE O-ring, 39 + 3.5 mm 17039

Other replacement parts are shown in the diagram.

170516985002PTFE connector

6985011 fluoro-elastomer O-ring, 39 + 3.5 mm

6985000 PTFE O-ring, 40 + 3.5 mm

6985001 PTFE filter support screen, 47 mm

O-ring, order no. 17039. The 6 mm outlet nipple is an integral part of the base, the 10 mm inlet hose nipple can be replaced by a Ga connector, order no. 17051.

Basic Filtration118 Combisart® manifolds

Made of high-grade stainless steel (B.S. 304S3 | AISI 304); accommodates any type of vacuum funnel. Stainless steel three-way valves (taps) allow the vacuum for each filter holder to be individually

Combisart® Manifolds 1-, 3- and 6-Branch

controlled and each holder to be sterilely vented. The low height of the manifold ports is particularly advantageous for working on a clean bench.

Ordering InformationCombisart® Manifolds, without Base Support and Frit Order No. Combisart® 1-branch manifold 16844

Combisart® 3-branch manifold 16842

Combisart® 6-branch manifold 16843

Combisart® Sets, Stainless Steel Capacity Order No.1-branch 1 + 100 ml 16844-CS

1-branch 1 + 500 ml 16845-CS

3-branch 3 + 100 ml 16824-CS

3-branch 3 + 500 ml 16828-CS

6-branch 6 + 100 ml 16832-CS

6-branch 6 + 500 ml 16831-CS

In each set stainless steel funnels with lids are preassembled.

Accessories and Replacement Parts

Description Pack Size Order No.Plug, conical, to close the venting hole beside the 3-way valve

10 6980225

Silicone O-ring for manifold female threads 3 6980235

Rubber tubing, 1 m 1 16623

Basic Filtration 119Combisart® manifolds

Glass Filter Holders Two compact vacuum filter holders for easy particulate ana lysis. Both the top and bottom part of the filter holders are easily and securely fastened together using the

metal clamp. The centering rim on the filter support ensures correct positioning of the membrane filter. The glass frit filter support guarantees uniform distribution of retained particles on the filter surface.

Ordering InformationDescription Order No.Glass filter holder Filter diameter Filtration area Capacity Outlet

30 ml 25 mm (or 24 mm) Prefilter, 20 mm 3 cm2

30 ml 12 mm outer diameter

16306

Glass filter holder Filter diameter Filtration area Capacity Outlet

250 ml 47 mm (or 50 mm) Prefilter, 40 mm 12.5 cm2

250 ml 15 mm outer diameter

16307

Adapter, 16836 | Adapter, 16837 For use of a glass filter holder, 16306 or 16307, on a Combisart® stainless steel manifold.

Glass Filter Holders; 30, 250 ml For Particle Counting

Ordering InformationDescription Order No. Adapter with 11 mm opening in stopper; for using filter holder 16306 on a Combisart® manifold

16836

Replacement stopper for 16836 00280 Adapter with 14 mm opening in stopper; for using filter holder 16307 on a Combisart® manifold

16837

Replacement stopper for 16837 00281

Basic Filtration120 Combisart® manifolds

Polycarbonate Filter Holders For Particle Counting

Polycarbonate Filter Holder, 250 mlThis reusable, practical filter holder made of autoclavable plastic is ideal for analytical testing outside the laboratory.For use with 47 mm membrane filters.

Outlet: TR 20 + 2 mm male thread

Ordering InformationDescription Order No.Polycarbonate filter holder without receiver flask 16511

Polycarbonate filter holder with receiver flask 16510Hand vacuum pump with gauge and 60 cm PVC tubing 16672

Basic Filtration 121Combisart® manifolds

Ready-to-Use Biosart® 250 Funnels For Particle Counting

Biosart® 250 Funnel The Biosart® 250 Funnel has been specially designed for analytical quality assurance. The sterile 250 ml plastic funnel guarantees fast filtration and high sample throughputs

during routine testing. Its large inner diameter allows high flow rates, and the tapered inner walls permit thorough flushing of the funnel, after filtration.

Ordering InformationDescription Order No. Biosart® 250 Funnel, 50 units, sterile-packaged 16407-25-ALK

Single Support, 16840 For adapting a Biosart® 250 Funnel for use on a Combisart® stainless steel manifold.

Ordering InformationDescription Order No. Stainless steel filter support for stainless steel manifold. 16840

Replacement

Description Order No. Stainless steel frit for 50 mm membrane filters 6980102Stainless steel frit for 47 mm membrane filters 6980103Silicone flat gasket underneath the frit 6980124PTFE flat gasket underneath the frit 6980104Silicone O-ring for 16840 male thread 6980274

Get an overview of our high-quality laboratory instruments, high-grade consumables and excellent services.

Our product portfolio focuses on laboratory instruments, such as lab balances, pipettes and laboratory water purification systems. Moreover, we offer the widest range of consumables like laboratory filters and pipette tips.

Did You know …… that you can order more lab catalogs?

Laboratory Water Purification Products

Laboratory Water Purification ProductsPublication No.: SL-0002-eOrder No.: 85037-548-54

Laboratory Filtration Products

Laboratory Filtration Products Publication No.: SLU0006-eOrder No.: 85032-543-83

Laboratory Weighing ProductsPublication No.: WL-0007-eOrder No.: 98649-018-13

Products for Microbiological Control

Products for Microbiological Control Publication No.: SL-1530-e Order No.: 85034-538-81

Laboratory Weighing Products

Sartorius Liquid Handling Products Publication No.: SUL0002-eOrder No.: 85032-542-25

Download our lab catalogs from our website: www.sartorius.com/lab-catalogs

Laboratory Weighing Products Sartorius Liquid Handling Products

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EuropeGermanySartorius Lab Instruments GmbH & Co. KGWeender Landstrasse 94–10837075 Goettingen

Phone +49.551.308.0Fax +49.551.308.3289

France & Suisse RomandeSartorius France2, rue Antoine Laurent de LavoisierZA de la Gaudrée91410 Dourdan

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[email protected]

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Phone +52.5555.62.1102Fax +52.5555.62.2942

Asia|PacificAustraliaSartorius Australia Pty. Ltd.Unit 5, 7-11 Rodeo DriveDandenong South Vic 3175

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Phone +86.10.8042.6300Fax +86.10.8042.6486

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Phone +852.2774.2678Fax +852.2766.3526

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Phone +91.80.4350.5250Fax +91.80.4350.5253

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Sales and Service ContactsFor further contacts, visit www.sartorius.com

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Laboratory Filtration Products · 10,000 MWCO 25 VS02H01 10,000 MWCO 100 VS02H02 30,000 MWCO 25...

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