LABORATORY DIAGNOSIS PERIPHERAL BLOOD SMEAR. A properly prepared blood smear is essential to...

LABORATORY DIAGNOSISLABORATORY DIAGNOSIS

PERIPHERAL BLOOD PERIPHERAL BLOOD SMEARSMEAR

PERIPHERAL BLOOD SMEARPERIPHERAL BLOOD SMEAR

A properly prepared blood smear is A properly prepared blood smear is essential to accurate assessment of essential to accurate assessment of cellular morphologycellular morphology

The wedge smear is the most convenient The wedge smear is the most convenient and commonly used technique for making and commonly used technique for making PBSPBS

PERIPHERAL BLOOD SMEARPERIPHERAL BLOOD SMEARWedge technique of making

PBS

A. Correct angle to hold spreader slide

B. Blood spread across width of slide

C. Completed wedge smear


Characteristics:Characteristics:– About two thirds to About two thirds to

three fourths of the three fourths of the slide is covered by the slide is covered by the smearsmear

– It is very rounded at It is very rounded at featheredge (thin featheredge (thin portion), not bullet portion), not bullet shapeshape

– Lateral edges of the Lateral edges of the smear should be smear should be visiblevisible

Well-made PBS


Characteristics:Characteristics:– It is smooth without It is smooth without

irregularities, holes, or irregularities, holes, or streaksstreaks

– When the slide is held When the slide is held up to light, the up to light, the featheredge of the featheredge of the smear should have a smear should have a “rainbow” appearance“rainbow” appearance

– The whole drop is The whole drop is picked up and spreadpicked up and spread

Well-made PBS


Examples of unacceptable smearsExamples of unacceptable smears


Staining of PBSStaining of PBSPurpose of staining is to identify cells and Purpose of staining is to identify cells and recognize morphology easily through the recognize morphology easily through the microscopemicroscope

Uses Wright stain or Wright-Giemsa stain which Uses Wright stain or Wright-Giemsa stain which contain both eosin and methylene blue contain both eosin and methylene blue polychrome stainspolychrome stains


Optimally stained smears have the Optimally stained smears have the following characteristics:following characteristics:

1. RBC’s should be pink to salmon in color

2. Nuclei are dark blue to purple

3. Cytoplasmic granules of neutrophils are lilac

4. Cytoplasmic granules of basophils are dark blue to black

5. Cytoplasmic granules of eosinophils are red to orange

6. The area between the cells should be clean and free of precipitated stain


Peripheral smear Peripheral smear examinationexamination

Correct area of blood in Correct area of blood in which to evaluate cellular which to evaluate cellular distribution and perform distribution and perform WBC estimateWBC estimate


Peripheral smear Peripheral smear examinationexamination

““Battlement” pattern for Battlement” pattern for performing a WBC performing a WBC differential countdifferential count

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