Laboratory Services

LAB.MICRO.300 Anaerobic and Aerobic Collection Guide

Copy of version 1.1 (approved and current)

Last Approval orPeriodic Review Completed 12/29/2017

Next Periodic ReviewNeeded On or Before 12/29/2019

Effective Date 8/31/2017

Controlled Copy ID 62016

Location Lab Test Catalog

Organization Memorial Health System

Comments for version 1.0 (last major revision)Initial version

Comments for version 1.1 (this revision)Added tissue; condensed photos.

Approval and Periodic Review Signatures

Type Description Date Version Performed By Notes

Periodic review Designated Reviewer 12/29/2017 1.1 Amery Ray

Approval Lab Director 3/26/2016 1.0 Cory Dunn

Approval Department Medical Director 3/24/2016 1.0 Nathan Johnston

Approval Manager Approval 3/21/2016 1.0 Patricia Dilley

Approval Supervisor Approval 3/18/2016 1.0 Amery Ray

Approval QA check 3/18/2016 1.0 Tianna McCormick

Version History

Version Status Type Date Added Date Effective Date Retired

1.1 Approved and Current Minor revision 8/31/2017 8/31/2017 Indefinite

1.0 Retired Initial version 3/14/2016 3/26/2016 8/31/2017

Anaerobic and Aerobic Culture CollectionPURPOSE

This process describes how to collect specimens for Anaerobic or Aerobic Culture

PROCESS For Anaerobic Culture Two (2) swabs are requested/collected. Use a sterile container for fluid specimens or tissue specimens. Do not submit fluid specimens or tissue specimens in swabs. Requests for anaerobic culture will always have aerobic culture and gram stain performed.

1. Aerobic Culture and Gram Stain

2. Anaerobic Culture

Suitable sources for Anaerobic Culture*: Aspirate (by needle and syringe) Bartholin’s gland/cyst and Boils, Skin Bone Marrow/Blood Bronchoscopy, Protected Brushing Culdocentesis (aspirate) Fallopian Tube (aspirate/biopsy) IUD (for Actinomyces species) Pilonidal Cysts Placenta via C-section delivery Sinus aspirate Surgical wounds and tissues (aspirate, tissue, swab) Transtracheal aspirate Urine, suprapubic aspirate

For Aerobic ONLY Culture One (1) swab is requested/collected. This does not include Gram Stain. Submit two (2) swabs if Gram Stain is desired.

*Murray, Patrick R., *Manual Clinical Microbiology, ASM, Current Ed, Washington, D.C. 2015, ASM Press

Microbiology Lab is available 24/7 for questions: 719-365-5686

Approved and current. Effective starting 8/31/2017. LAB.MICRO.300 (version 1.1) Anaerobic and Aerobic Collection Guide

Controlled copy ID 62016. Printed on 1/3/2018 10:34 AM (MST). Page 1 of 1