La Spada Lab Meeting 08.05.15
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LA SPADA LAB MEETING 08.04.15 Jahnavi Singh
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Transcript of La Spada Lab Meeting 08.05.15
LA SPADA LAB MEETING 08.04.15Jahnavi Singh
NPC QPCR RESULTSNDUFS3
0
1
2
3
4
5
Control 50Q 65Q 70Q
PGC1a
01
23456
7
Control 50Q 65Q 70Q
*
*
*
*
*ACADM
0
1
2
Control 50Q 65Q 70Q
CYCS
0
1
2
Control 50Q 65Q 70Q
GPX1
0
1
2
3
4
Control 50Q 65Q 70Q
*
***
*Cox6A1
0
1
2
3
4
5
Control 50Q 65Q 70Q
***
Cox7C
0
1
2
3
4
Control 50Q 65Q 70Q
*
CAT
0
1
2
Control 50Q 65Q 70Q
SOD1
0
1
2
3
4
5
Control 50Q 65Q 70Q
**
SOD2
0
1
2
3
Control 50Q 65Q 70Q
UCP2
0
1
2
3
Control 50Q 65Q 70Q
TFAM
0
1
2
Control 50Q 65Q 70Q
Sirt6
0
1
2
3
4
Control 50Q 65Q 70Q
MOUSE RETINA QPCR RESULTSCox4i1
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
TFAM
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
ACADM
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
CYCS
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7SOD1
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
SOD2
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
Cox6A
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
GPX
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
UPC2
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
CAT
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
PGC1a
0.00
0.25
0.50
0.75
1.00
1.25
WT SCA7
SUMMARY
Increased regulation of ROS Defense genes in Complex 4 of the mitochondrial ETC
Upward trend associated with number of CAG repeats in NPC qPCR results
Future plans: run Mouse Retina
TECHNIQUES USED
DNA Cloning and Bacterial Transformation
PCR Amplification
Mitochondrial Morphometry and Total Fluorescence (Fiji, ImageJ Analysis and Microscope)
RNA Extraction/ cDNA Generation of mouse retina and SCA7 NPC cells
qPCR assays
BCA and Western Blot
