09:00 - 09:25 i KL25 i BIOMARKERS OF (ANTI)OXIDANT STATUS IN HUMAN NUTRITION, AGINGAND DISEASEEugene Jansen

Canada

TheNetherlands

09:25 - 09:50 i IL26 i A GENETIC IMMUNONUTRITION RESEARCHEXPERIENCERanda Reda Mabrouk

Egypt

09:50 -10:05 i YSL27 i IMPACT OF ElEVATED LEVELSOF PLASMA HOMOCYSTEINE ANDIMPAIRED DNA REPAIR IN A MOUSE MODEL OF VASCULAR COGNITIVE

IMPAIRMENTNafisa JadavjiCoffee break

COMPARISON OF THE POLYPHENOLlC PROFILES OF BERRIES FROMDIFFERENT VACCINIUM SPECIESIN RELATION TO THEIR NUTRACEUTICAL

VALUEClaudia Anciilotti

Portugal

11:35 - 11:50 i YSL30

Lunch break

Italy

ATHENA STUDY: ANTIOXIDANTS AND CARDIOVASCULAR RISKCaterina Brasacchio

11:50 -12:05 i YSL31 Italy

12:05 -12:20 i YSL32 INTESTINAL TRANSPORT OF SARDINE (SARDINE PICHARDUS) PROTEINHYDROLYSATE PREPARED FROM ACTION OF SACCHAROMYCES BREWINGYEAST SURPLUS PROTEASES- ASSESSMENT OF ANTIOXIDANT AND ACE-INHIBITORY ACTIVITIESElsa Vieira

12:20-13:20Poster Session iii

l#i1#il1P.JIJI!I!.. 1:.:"'.1111 ."1.13:20 -14:20

(-)-CARVONE REDUCED INTRACELLULAR TRIGLYCERIDE ACCUMULATIONVIA ACTIVATION OF OR1Al1N CULTURED HEPATOCYTES

Sung Joon Lee

Korea14:20 - 14:40 i L33

DATE SEEDS:PROXIMATELY COMPOSITION AND BINDING OF BILE ACID IN

VITROGülden Gökşım

14:40 - 14:55 i YSL34Turkey

14:55 -15:15 i L35 COCONUT CONSUMPTION AND CARDIOVASCULAR DISEASEINCIDENCE, ISTHERE AN ASSOCIATION? A CASE CONTROL STUDY IN SRI LANKAN

POPULATIONLathika Athauda

Sri Lanka

15:15 -15:35 Coffee breakSocial Programme18:00 - 21:00

6

10:15 - 10:30

BENEFICIAL EFFECTOF LOW DOSEOF ETHANOL ON THE SENESCENCEIN SENESCENCE-ACCELERATEDMICE JapanNorihisa Kato

09:25- 09:50 L37 i NEW TRENDS IN BIOLOGICAL ACTIVITY DETECTION - RELATION TONUTRITION AND DIAGNOSTICS i Czech RepublicJan Krejci

9:50 -10:15 L38 i DIOXINs IN THE.FODDER OF INTENSIVE LlFESTOCKFARMING? i AustriaHans Peter SchıeferClosing ceremony - Best Poster and Best Young Scientist Presentation Award.Invitation to the 16th INDC

10:30 - 11:30 Farewell cocktall

7

IL - Invited LectureKL- Keynote LectureL- LectureOL - Opening LectureYSL- Young sclentlst's Lecture

L33 (-)-CARVONE REDUCED INTRACELLULAR TRIGLYCERIDE ACCUMULATION VIAACTIVATION OF OR1A11N CULTURED HEPATOCYTES

Lee S. J. (1), Wu C. (1), Jia Y. (1), Kim Y. (1)

(1) Department of Biotechnology, Graduate School of Life Sciences and Biotechnology, BK21-PLUS,Korea University, SeouI136-713, Republic of Korea

E-mail: junelee@korea.ac.kr

Keywords: (-)-carvone, arama compound, olfactory receptor, lipid metabolism, liver

Aroma compounds in plants show biological activities interacting with cellular receptors and regulators.Here we show that (-)-carvone, a major aroma compound in spearmint reduces intracellulartriglyceride concentrations. (-)-carvone is known as a ligand to a human olfactory receptor, OR1A1. Inthis study, OR1A1 expression was detected in HepG2 liver cells. OR1A1 activation by (-)-carvoneincreased the cyclic adenosine monophosphate (cAMP), but not intracellular Ca2+ concentration,thereby inducing protein kinase A (PKA) activity with subsequent phosphorylation of cAMP responseelement-binding protein (CRES) and upregulation of the CRES-responsive gene hairy and enhancerof split (HES)-1, a corepressor of peroxisome proliferator-activated receptor-y (PPAR-y) inhepatocytes. In (-)-carvone-stimulated cells, the repression of PPAR-y reduced the expression of thetarget gene, mitochondrial glycerol-3-phosphate acyltransferase, which encodes a key enzymeinvolved in triglyceride synthesis. Intracellular triglyceride level and lipid accumulation were reduced incells stimulated with (-)-carvone, effects that were diminished following the los s of OR1A1 function.These results indicate that OR1A 1 may function as a non-redundant receptor in hepatocytes thatregulates the PKA-CRES-HES-1 signaling axis and thereby modulates hepatic triglyceridemetabolism.

Acknowledgements: This work was conducted with the support of the "Cooperative Research Programfor Agriculture Science & Technology Development" (Project No. PJ01009003) of the RuralDevelopment Administration, Republic of Korea.

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L34 DATE SEEDS: PROXIMATEL Y COMPOSITION AND BINDING OF BILE ACID IN VITRO

Gülden G. /1l, Özge D. (1), Sedat S. (1), H. İbrahim E. (1)

(1) Department of Food Engineering, University of Mersin, 33343, Mersin, Turkey

E-mail: gulgok33@gmail.com

Keywords: dateseeds, dietaryfiber, antioxidant, bile acid binding

Introduction: In the past few years there have been attempts to use agricultural by-products forpotential health benefits in the diet formulation of human nutrition. Date seeds are by-products of date(Phoenix dactylifera) serve as good sources of dietary fiber compared with most fresh, dried fruits andtheir by-products. Increased intake of dietary fiber has been found to alter gut transit time, alert thecolonic microflora, increase feces weight, decrease appetite effects, absorb toxins and bind bile acids.The bile acids are known amphiphilic steroids which are synthesized from cholesterol in the liver. Theadsorption capacity of bile acids by dietary fiber is known regarding the mechanisms for thehypocholesteremic effect of dietary fiber and an increase in the fecalloss of bile acids. Objectives: Themain objectives of this study were the determination of the proximate composition and the nutritionalproperties of date seed. Thus, protein, fat, soluble and insoluble dietary fiber, lignin, mineralcompositions were analyzed. Additionally, total phenolic and antioxidant content and bile acid bindingcapacity of date seeds were also determined.Material and Methods: Date seeds were obtained fromdate palm three cultivars (Safawi, Sughai, Mabrum) grown in Madinah, Saudi Arabia. The dry matter,protein, lipid and ash content of date seeds were determined by us ing standard AOAC methods(AOAC, 2000). Soluble and insoluble dietary fiber was determined according to Prosky (1987) byusing the Megazyme total dietary fiber analysis kit (Megazyme International Ireland Ltd, Wicklow,Ireland). The method given by Sayar (2005) was applied for the determination of bile acid bindingcapacity. Results: The protein, fat, ash and total dietary fiber contents of the date seeds varieties(Safawi, Sughai and Mabrum) were ranged from 5.42-5.60, 6.99-8.14, 0.81-0.97 and 85.43-86.41g/100 9 dry weight, respectively. The potassium concentration was the highest, followed in deseendingorder by magnesium and phosphorus. Safawi date seed had the highest amount of total phenolics(48.32 mg GAE/g DM) and DPPH radical scavenging activity (6.17 mg TROLOX E/g DM). The bileacid binding activity of Safawi, Sughai and Mabrum date seeds were found to be 7.02, 7.67 and 6.96mmol/l00g respectively. Conclusions: Date seeds can provide more dietary fiber in the diet than waspreviously believed. There could be relationship between SA binding ability and dietary fiber contentaccording to these findings.However, micro and macro nutrients of date seeds to a wide range ofprOducts will contribute to the development of value-added or functional and technological foods thatrecenlly are in high demand. This confirms that the date seeds has great potential in food applications,especially in development of functional foods.

References

(l)AOAC, 2000. Official Methods of Analysts, 18 the drı., Assoc. Off. AnaL. Chem., Wash. D.C., USA.(2) Nardi, E. P., Evangelista, F. S., Tormen, L., Saint, T. D., Curtius, A. J., de Souza, S. S., & Sarbosa,

F. (2009). The use of inductively coupled plasma massspectrometry (ICP-MS) for the determinationof toxic and essential elements in different types of food samples. Food Chemistry, 112(3), 727-732.

(3) Prosky, L., Asp, N. G., SChweizer, T. F., DeVries, J. W., & Furda, I. (1987). Determination ofinsoluble, soluble, and total dietary fiber in foods and food products: inter laboratory study. Journal-Association of Official Analytical Chemists, 71(5),1017-1023.

(4) Thaipong, K., Soonprakob, U., Crosby, K., Cisneros-Zevallos, L., & Syrne, D. H. (2006).COmparison of ASTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity fromguava fruit extracts. Journal of food composition and analysis, 19(6), 669-675.

(5) Sayar, S., Jannink, J. L., & White, P. J. (2005). In vitro bile acid bindingoffloursfromoat lines varyingin percentage and molecularweightdistributionof 13-glucan. Journal of agricultural and foodchemistry, 53(22), 8797-8803.

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P91 A HIGH PROTEIN DIET AND ISOMETRIC EXERCISES IMPROVE BODY COMPOSITION INER+ BREAST CANCER PATIENTS TREATED WITH ANTIESTROGENIC MEDICATION

Artene D. (1), BHdaru A. (2), Bordea C. i. (2)

(1) Carol Davila Medicine University, Bucharest, Romania(2) Surgical Oncology Department of Surgery at Carol Davila Medicine University, Oncology

Department at Alexandru Trestioreanu Institute of Oncology, Bucharest, Romania

E-mail: artene.diana@yahoo.com

Keywords: breast caneer, high protein diet, isometric exercises, body composition

Many breast cancer patients gain weight during treatment increasing recurrence, oncology specificmortality and general mortality risks. Breast cancer diagnosis and treatment overthrow patients'lifestyle aggravating sedentariness and any preexisting weight gain causes like insulin and leptinresistance, dysbiosis and dyslipidemia.The aim of this study is to evaluate the efficiency of a high protein diet - based on foods naturally highin proteins, omega-3 fatty acids, calcium, probiotics and prebiotics - and of an isometric exe re iseinterventions to generate fat los s without muscle los s in ER+ breast cancer patients takingantiestrogenic treatmenı'We randomized 50 ER+ breast cancer patients - taking either Tamoxifen or Aromatase Inhibitors (AI)after surgery and chemotherapy - to follow the high protein diet, or the diet and 4' isometric exercisesper day for 8 weeks. Patients were instructed to eat only when hungry and to keep a food journal. Wemeasured weight and body composition with a bioelectrical impedance scale after checking forhydration status.The diet group lost 2.17 ± 2.42% body fat (p = 0,000) with no muscle loss, and there was no statisticaldifference between patients taking Tamoxifen or AI regarding body composition evolution. The diet +isometric exercise group lost 2.2 more pounds than the diet only group and 0.66 ± 0.91% visceral fat(p = 0,001) also with no muscle loss. AI patients from the diet and exercise group did not improvemuscle mass - maybe because of the musculoskeletal impact of AI medication.In conclusion, a high protein diet can decrease body fat in ER+ breast cancer patients onantiestrogenic medication. Adding a daily minimal exercise protocol to a high protein diet decreasesvisceral fat - which is more hormonally active. And resistance-training exercises are more appropriatefor patients on AI.

References

(1) Barron, C., Tsiarıi, E., and Tsakiridis, T. (2012, June). Expression of the glucose transportersGLUT1, GLUT3, GLUT4 and GLUT12 in human cancer cells. In BMC Proceedings (VoL. 6, No.Suppl 3, p. P4). BioMed Central Ltd.

(2) Batterham, R L., Heffron, H., Kapoor, S., Chivers, J. E., Chandarana, K., Herzog, H., ... & Withers,D. J. (2006). Critical role for peptide yy in protein-mediated satiation and body-weight regulation.Cell metabolism, 4(3), 223-233.

(3) Ju, Y. H., Doerge, D. R, Alıred, K. F., Alıred, C. D., and Helferich, W. G. (2002). Dietary genisteinnegates the inhibitory effect of tamoxifen on growth of estrogen-dependent human breast cancer(MCF-7) cells implanted in athymic mice. Cancer Research, 62(9), 2474-2477.

(4) Knols, R., Aaronson, N. K., Uebelhart, D., Fransen, J., and Aufdemkampe, G. (2005). Physicalexercise in cancer patients during and after medical treatmenI: a systematic review of randomizedand controlled clinical trials. Journal of clinicaloncology, 23(16), 3830-3842.

(5) Lin, Jennifer, et aL. "Intakes of calcium and vitamin D and breast cancer risk in women." Archives ofInternal Medicine 167.10 (2007): 1050-1059.

Acknowledgements: Project co-financed by the European Social Fund Operational ProgrammeHuman Resources Development 2007-2013 - POSDRU/159/1.5/S/155463

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P92 ANTI-ESCHERICHIA COLl ACTIVITY AND ANTI-MUTAGENIC ACTIVITY OF PHOENIXDACTYLlFERA SEEDS

Gülden G. (1), A. Elit E. (1), H. ibrahim E. (1)

(1) Department of Food Engineering, University of Mersin, 33343, Mersin, Turkey

E-mail: gulgok33@gmail.com

Keywords: Dateseeds, Umu-test, Anti-Escherichia coli activity, Healtheffects

In the pa st few years there have been attempts to use agricultural by-products for potential healthbenefits in the diet formulation of human nutrition.For Muslims all over the world dates are used asfood about 6000 years and are mentioned importantly in many times in the Quran.The date seedshave possed extractible high value components for safely human consumption as functional foods andingredients in nutraceuticals. Date seeds represent about 10-15% of the date fruits weight and havephytochemical compositions and good nutritional values. However, date seeds are utililized asdomesticated animal feed and waste and have very little and inadequate use at presenI. Date seedshave been scientifically demonstrated to have therapeutic implications against such as diabetes,hyperlipidemia, obesity, cholesterol, coloncancer and intestinaldisordersdue to high dietary fibercontents 'and bioactive constituents. Additionly date seeds have antiviral, gastrointestinal protective,hepatoprotective and nephroprotective activities at preclinical studies. The seeds of date include highamounts of bioactivecompounds (polyphenolic antioxidants and dietary fiber), which may have positivehealth effects and contribute to preventing various infectious diseases. In the study, the anti-Escherichia coli activity and anti-mutagenic activity of date seeds water extract from date palm threecultivars grown (Safawi, Sughai, Mabrum) in Madinah from Saudi Arabia were assessed bydiscdiffusionmethod and Umu-test using S.typhimuriumTA1535/pSK1002, respectively. The MICvalues of date fiber extracts on E. coli were determined 0.26, 0.51, 1.03 mg/mL for Safawi, Sughai andMabrum, respectively. Acording to Umu-test, Mabrum, Safawi and Sughai were highly suppressed67.6%, 56.6%, 14.4% of the mutagenicity of furylfuramide(S9-) and 3.6%, 43.1%, 16.5% of themutagenicity of 2-aminoanthracene (S9+), respectively.

References

(1) Vanden-Berghe, D. A., & Viietinek, A. J. Screening methods for antibacterial and antiviral agentsfrom higher plants 1991. Methods in Plant Biochemistry. Academic Press, London.

(2) Yamamoto, A., Kohyama, Y., & Hanawa, T. (2002). Mutagenicity evaluation of forty-one metal saltsby the umu test, Journal of biomedical materials research,59(1), 176-183.

(3) McFarland, J. (1987). Standardization of bacterial culture for the disc diffusion assay. J Am MedAssoc, 49, 1176-1178.

(4)AI-Farsi, M. A., & Lee, C. Y. (2008). Optimization of phenolics and dietary fibre extraction from dateseeds. Food Chemistry, 108(3),977-985.

(5)AI-Shahib, W., & Marshall, R J. (2003). The fruit of the date palm: its possible use as the best foodfor the future? Internationaljournal offood sciences and nutrition, 54(4), 247-259.

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