KOHJIN BIO CATALOGUE€œKOHJIN“, as written in Kanji letters, means “The organization group of...

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KOHJIN BIO CATALOGUEKOHJIN BIO CATALOGUE

KohjinKohjin Bio Co., Ltd.Bio Co., Ltd.

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Our mission and basic philosophy

After the experience of laboratory animal's rearing management for over 10 years, we have been handling the manufacture and sales of animal blood and sera. At our initial foundation period we have won over a lot of trusts through biotechnology service business, particularly establishing the position as the raw material supply base of the monoclonal and polyclonal antibodies.

Recently, we have been working on the development and manufacturing of microbiological media as well as tissue culture media even while industrial infrastructures of biotechnology was expanding, we especially concentrate on making development of the tissue culture media and challenging to a new creation at a vision.

““KOHJINKOHJIN““, as written in Kanji letters, means “The organization group of those whoalways consider”.

Our basic philosophy is as follows:(1) It is always understood to have worked by the

wholeheartedly. (2) We have developed and released the state-of-

the-art products that are necessary to the world. (3) We are establishing a company filled with a lot

of pleasure.

President/CEO : Takahito Nakamura

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Company profile

Company Name: Kohjin Bio Co., Ltd.

Address: 5-1-3 Chiyoda, Sakado city,Saitama 350-0214

President/CEO : Takahito Nakamura

Capital: JPY 105 million

Establishment: April 20, 1981

Number of Employees: 86

Dealing Banks:

Mitsubishi Tokyo UFJ, Sakado branch

Sumitomo Mitsui Bank, Sakado branch

Saitama Resona Bank, Sakado branch

Musashino Bank

Hanno Shinkin Bank Headquarters

Major Business Activities:・ Manufacturing and sales of animal blood, sera, tissue culture

media, agar media for microorganism tests, in vitro diagnostic agents, and cosmetics

・Sales of medical equipments・Trust of animal immunization and the following antibody

preparation・Import and export operations of products and raw materials

Permits and Approvals:・Medicine manufacturing permit

・The second-class medicine sales permit

・Cosmetics-manufacturing permit

・Poisonous and deleterious substances-manufacturing registration

・Poisonous and deleterious substances import registration

・Medical equipment-manufacturing permit

・The third-class medical equipment-manufacturing sales permit

・Medical equipment-repairing permit

　

ISO 9001: 2008 certificated

ISO 13485 certificated

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29

YEARS

History

1981 Established as Kohjin Co., Ltd. at Suginami-ku, Tokyo, with the capital of JPY 10,000,000. Started on manufacture of animal blood/sera and culture media for bacterial test.

1982 Built a factory at Hanakage-cho, Sakado, Saitama and the manufacturing base moved there.

1986 Increased the capital to JPY 20,000,000. Built a new factory at Asabano, Sakado and started the manufacture of tissue culture media. Obtained permission as “Poisonous and deleterious substances manufacturing permit”

1989 Renamed to Kohjin Bio Co., Ltd. Increased the capital to JPY 28,000,000. Launched on production service of customer-oriented monoclonal and polyclonal antibodies.

1991 Increased the capital to JPY 48,000,000. Started manufacturing in vitro diagnostic agents. Obtained Medicine manufacturing permit.

1996 Moved the head office to Asabano. Increased the capital to JPY 70,000,000.

1997 Received the authorization on “Promotion of a creative action by small- and medium-sized businesses” from Saitama Prefecture.

1998 Received the commendation for “Technology development” from the Technology Development Foundation.

2000 Received the authorization on “Small- and medium-sized business innovation” from Saitama Prefecture.

2001 Built a new factory at the present address (Chiyoda, Sakado) and the manufacturing base moved there together with the head office.

2003 Acquired ISO 9001. Obtained the first to receive the authorization on “Management innovation model enterprise” from Saitama Prefecture.

2005 Increased the capital to JPY 98,000,000. Obtained cosmetics manufacturing permit.

2006 Acquired ISO 13485. Launched on development and manufacture of normal human dermal keratinocytes.

2007 Established Osaka office.

2008 Increased the capital to JPY 105,000,000.

2009 Obtained medical equipment manufacturing permit, the third-class medical equipment manufacturing sales permit, and the third-class medical equipment manufacturing sales permit. Establish Kohjin Japan Medical Biology Technological (Beijing) Co., Ltd as 100% subsidiary company in Beijing China.

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Contents

Major Products

KBM Serum-free media　KBM200 series　KBM300 series　KBM500 series　KBM700 series

KM Bankers (Cryopreservatives) Embryo culture kits Classical media Mouse embryonic stem cell culture

medium In vitro fertilization and embryo culture

kits Classical media Analytical equipment (outside product)

Price List

KBM Serum-free media　KBM200 series　KBM300 series　KBM500 series　KBM700 series

Culture-related products Analytical equipment (outside product) Normal human cell (culture media) Mouse embryonic stem cell culture medium In vitro fertilization and embryo culture kits Classical media Sera, plasma, and blood (outside products)

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Price List

KBM serum-free media

KBM 200 series for virus or recombinant protein productionProduct Name Form Packaging Product Code Unit Price Appliction

Liquid 500 mL 16022205 JPY 6,800Liquid 1000 mL 16022200 JPY 9,500Liquid 10 L 16022206 Need inquiryLiquid 500 mL 16022305 JPY 7,000Liquid 1000 mL 16022300 JPY 10,000Liquid 10 L 16022306 Need inquiryLiquid 500 mL 16022405 JPY 7,800Liquid 1000 mL 16022400 JPY 13,000Liquid 10 L 16022406 Need inquiry

KBM270 Liquid 500 mL 16022705 JPY 6,800 Monolayer CHO cellsProtein production

KBM220

KBM230

KBM240

Monolayer MDCK cellsVirus production

Monolayer Ｖｅｒｏ cellsVirus production

Suspended CHO cellsProtein production

KBM 300 series for cell fusion and hybridoma cultureProduct Name Form Packaging Product Code Unit Price Appliction

Liquid 500 mL 16023005 JPY 7,500Liquid 1000 mL 16023000 JPY 12,000

KBM306 Liquid 500 mL 16009025 JPY 8,500Cultures from cell fusionto establishment ofhybridoma cells

Expansion culture ofestablished hybridoma cellsKBM300

KBM 700 series for recombinant protein production by insect cellsProduct Name Form Packaging Product Code Unit Price Appliction

Liquid 500 mL 16027105 JPY 3,000

Liquid 1000 mL 16027100 JPY 5,600Sf-9, BmN, and N4H cellsderived from cabbage mothKBM710

KBM 500 series for activation and expansion culture of humanlymphocytes

Product Name Form Packaging Product Code Unit Price ApplicationLiquid 500 mL 16025015 JPY 20,000

Liquid 1000 mL 16025020 JPY 16,000

Liquid 1000 mL 1602502B JPY 17,500

Liquid 1000 mL 16025310 JPY 12,000

Liquid 500 mL 16025405 JPY 9,000Liquid 1000 mL 16025400 JPY 15,000

Liquid 1000 mL 1602540B JPY 16,500Gas-permeable bag containing KBM540Liquid 1000 mL 16025410 JPY 12,000

Liquid 1000 mL 16025500 JPY 15,000 Activation and expansion

Liquid 350 mL 1602550C JPY 10,000Liquid 1000 mL 1602550A JPY 16,500

Liquid 1000 mL 16025510 JPY 12,000

Liquid 1000 mL 16025600 JPY 15,000

Liquid 1000 mL 1602560A JPY 16,500

Liquid 1000 mL 16025610 JPY 12,000

Liquid 1000 mL 16025710 JPY 12,000

Activation and expansion(add IL-2)

Expansion

Depleted of IL-2 from KBM550Activation and expansion(add IL-2)

Not containing both albumin and IL-2Activation and expansion(add IL-2)

Activation and expansion

Expansion

KBM501

KBM53１

KBM541

KBM551

KBM502

KBM502B

Containing human albumin and 1,750,000 JRU/L IL-2Activation

Containing human albumin and 175,000 JRU/L IL-2Activation

Gas-permeable bag containing KBM502Expansion

KBM540

KBM540B

Containing human albumin and 175,000 JRU/L IL-2

Gas-permeable bag containing KBM560

Depleted of IL-2 from KBM540

Containing human albumin and 175,000 IU/L IL-2

Expansion

KBM550

KBM550BA

Containing human albumin and 175,000 IU/L IL-2

Gas-permeable bags containing KBM550

KBM560

KBM560BA

Activation and expansion

KBM571

KBM561Depleted of IL-2 from KBM560

Activation and expansion(add IL-2)

Containing human albumin but not IL-2Activation and expansion(add IL-2)

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Price List

Culture-related products

CryopreservativesProduct Name Form Packaging Product Code Unit Price DescriptionKM Banker Liquid 120 mL 16092005 JPY 12,000 Chemically definedKM Banker II Liquid 120 mL 16092010 JPY 12,000 Containing human albumin

Gas-permeable culture bags

Medium supplementProduct Name Form Packaging Product Code Unit Price DescriptionBovine pituitarygland extract

Lyophilizate 15 mg 16500100 JPY 8,000 Extracted from New Zealand bovinesSustitute for basic FGF or other growth factors

Product Name Qty. Package Product Code Unit Price Direction/DescriptionMaccell 1 1 16087500 JPY 5,000Maccell 1 10 16087501 JPY 39,000Maccell 10 1 16087504 JPY 39,000Maccell II 1 1 16087517 JPY 5,000Maccell II 1 10 16087518 JPY 39,000Maccell II 10 1 16087516 JPY 39,000Maccell A 1 1 16087530 JPY 5,000Maccell A 1 10 16087531 JPY 39,000Maccell A 10 1 16087532 JPY 39,000Maccell II-A 1 1 16087526 JPY 5,000Maccell II-A 1 10 16087527 JPY 39,000Maccell II-A 10 1 16087528 JPY 39,000

TAZETTA-F 1 10 16087520 JPY 35,000

Use for 25- to 300-mL suspension culture.Cells grow in the bag under the environmentalconditions of 5% CO2 plus 95% air at 37Cbecause the bag is made with gas-permeablethin plastic membrane.

Partition clip 10 1 16087523 JPY 12,000

Use for partitioning a whole culture room of aTAZETTA bag into two or three small rooms.The net volume is increased by removing theclump(s) with increasing the number of cellsduring culture, decreasing the cell concentrationwith medium.

Use for semi-large scale suspension culture upto 1500 mL.Cells grow in the bag under the environmentalconditions of 5% CO2 plus 95% air at 37Cbecause the bag is made with gas-permeablethin plastic membrane.

EquipmentProduct Name Product Code Kit Price Application

TeraScan VPC 19925010 JPY 7,284,000 Cytotoxicity test

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Price List

Media for normal human cellsProduct Name Form Packaging Product Code Unit Price DescriptionSerum-Free Medium for NormalHuman Epidermal Keratinocytes Liquid, frozen 500 mL 16087541 JPY 18,000

Containing pituitary gland extract derivedfrom New Zealand bovines

Medium for Normal HumanVascular Endothelial Cells Liquid, frozen 500 mL 16087542 JPY 22,000

Containing fetal bovine serum, epidermalgrowth factor, and endothelial growth factorsupplement

Medium for embryonic stem cells

Product Name Form Packaging Product Code Unit Price DirectionLiquid, frozen 250 mL 16089102 JPY 8,000Liquid, frozen 500 ｍL 16089105 Need inquiry

Serum-Free Medium for MouseEmbryonic Stem Cells

Add leukemia inhibitoryfactor.

Normal human cellsProduct Name Form Packaging Product Code Unit Price

Normal Human Dermal Keratinocytes Suspension, frozen at-80C while delivering 1 106 cells 16087540 JPY 36,000

Normal Human Umbilical VenousEndothelial Cells

Suspension, frozen at-80C while delivering 1 106 cells 17510140 JPY 30,000

In vitro fertilization and embryo culture kits

Product Name Form Packaging Product Code Unit Price DescriptionARTEC AH-25 Liquid 25 mL 6 16087960 JPY 9,000 Basal embryo culture mediumARTEC B-25 Liquid, frozen 0.3 mL 6 16087970 JPY 5,000 Additive to the basal mediumARTEC C1-25 Liquid 4.7 mL 6 16087962 JPY 10,000 Serum substitute for culture at 2-to 8-cell stagesARTEC C2-25 Liquid, frozen mL 6 16087963 JPY 10,000 Serum substitute for culture at 8-cell to blastcyst stagesARTEC D-25 Liquid, frozen 0.3 mL 6 16087964 JPY 5,500 Supplement for culture at 8-cell to blastcyst stagesARTEC E-25 Liquid, frozen 0.2 mL 6 16087965 JPY 5,500 Supplement for culture at 8-cell to blastcyst stagesARTEC F-500 Liquid 500 mL 6 16087971 JPY 12,000 Mixture of external nutrient medium and a gas environment stabilizer

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Price List


Physiologically isotonic buffer solutionsProduct Name Form Packaging Product Code Unit Price Description

Liquid 500 mL 16005550 JPY 1,600Powder 1 L 10 16232001 JPY 3,700Powder 10 L 16232000 JPY 2,600

10-fold ConcentratedD-PBS（-）

Liquid 500 mL 16005510 JPY 3,100

Phosphate-Buffered Saline Liquid 500 mL 16008505 JPY 1,600 Sterilized by 0.1-m membranefiltration

HBSS(-)without Ca2+ and Mg2+ Liquid 500 mL 16229015 JPY 1,600 Depleted of Ca2+ and Mg2+

Containing phenol redHBSS（＋）with Ca2+ and Mg2+

without Phenol RedLiquid 500 mL 16230020 JPY 2,800 Containing Ca2+ and Mg2+

Depleted of phenol red

D-PBS（-）without Ca2+ and Mg2+

Depleted of Ca2+ and Mg2+

SupplementsProduct Name Form Packaging Product Code Unit Price Direction100-fold ConcentratedMEM Vitamin Solution

Liquid, frozen 100 mL 16222004 JPY 2,800 Add a 1% volume of the solution to preparing Eagle's MEMor to culture for nutritional replenishment.

50-fold ConcentratedMEM Essential Amino Acid Solution

Liquid, frozen 100 mL 16223004 JPY 4,200Add a 2% volume of the solution to preparing MEM or to culture fornutritional replenishment.

100-fold ConcentratedMEM Non-Essential Amino Acid Solution

Liquid, frozen 100 mL 16224004 JPY 3,000Add a 1% volume of the solution to preparing MEM or to culture fornutritional replenishment.

L-Glutamine Solution （200mM） Liquid, frozen 100 mL 16202004 JPY 3,900 Add a 1-2% volume of the solution to any preparing mediumor to culture for nutritional replenishment.

Sodium Hydrogen Carbonate Solution（7.5%）

Liquid 100 mL 16201004 JPY 2,500 Add an appropriate volume of the solution to any preparing mediumat the indicated concentration.

Penicillin-Streptomycin Solution(50,000 U/mL-50 mg/mL）

Liquid, frozen 10 mL 16210001 JPY 3,000 Add a 1% volume of the solution to culture to inhibit growth ofbacteria.

HEPES Buffer Solution （1M） Liquid 100 mL 16203004 JPY 12,000 Add an appropriate volume of the solution at less than 25 mMto any preparating medium to achieve the desired pH.

50-fold Concentrateed HAT Solution Liquid, frozen 100 mL 16213004 JPY 4,000 Add a 2% volume of the solution to culture to eliminate unfusedor self-fused myeloma cells during hybridoma selection culture.

50-fold Concentrated HT Solution Liquid, frozen 100 mL 16214004 JPY 3,200 Add 2% volume of the solution to culture after unfused or self-fusedmyeloma cells are eliminated.

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Price List


Cell dissociation reagentsProduct Name Form Packaging Product Code Unit Price Direction

Trypsin Solution (0.25%) Liquid, frozen 100 mL 16204004 JPY 3,000

Before use, mix the solution with anequal volume of EDTA solution (0.1%)aseptically.Dilute 2.5 times with D-PBS(-).

Trypsin Solution (2.5%) Liquid, frozen 100 mL 16205004 JPY 6,000

Before use, mix the solution with anequal volume of EDTA solution (0.4%)aseptically.Dilute 10 times with D-PBS(-).

EDTA Solution (0.02%) Liquid 100 mL 16206004 JPY 2,000

Dissolve trypsin (1:250) powder atconcentrations of 0.05-0.2% inEDTA solution (0.02%). Sterilizethe solution by membrane filtration.

EDTA Solution (2%) Liquid 100 mL 16207004 JPY 2,800

Dilute 100 times with D-PBS(-) first.Dissolve trypsin powder (1:250) atconcentrations of 0.05-0.2% in thediluted EDTA solution.Sterilize the solution by membranefiltration.

EDTA-Trypsin Solution(0.02%-0.1%) Liquid, frozen 100 mL 16208004 JPY 2,800 Use the solution as it is.

OthersProduct Name Form Packaging Product Code Unit Price Application

Trypan Blue Solution (0.5%) Liquid 100 mL 16209004 JPY 2,800 Cell viability testPyrogen-Free Ultra Pure Water Liquid 1000 mL 16216010 JPY 3,400 Medium preparation

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Price List

Classical media

RPMI 1640Product Name Form Packaging Product Code Unit Price

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Liquid 500 mL 16005005 JPY 1,600

Liquid 500 mL 16005050 JPY 1,600

Note

RPMI 1640

Ham's F-12 (F-12 Nutrient Mixtures) Product Name Form Packaging Product Code Unit Price

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Liquid 500 mL 16006255 Need inquiry

Liquid 500 mL 16006250 JPY 1,600

Note

Ham's F-12

DMEM （Dulbecco's Modified Eagle’s Medium）Product Name Form Packaging Product Code Unit Price

DMEM

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Liquid 500 mL 16003005 JPY 1,600 Liquid 500 mL 16003550 JPY 1,600 Liquid 500 mL 16003505 JPY 1,600

Liquid 500 mL 16003560 JPY 1,600

Note

Low glucose

High glucose

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Price List

Classical media

Eagle’s MEM （Minimum Essential Media Eagle）Product Name Form Packaging Product Code Unit Price

MEM Eagle

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Liquid 500 mL 16002005 JPY 1,600


Liquid 500 mL 16002025 Need inquiry Liquid 500 mL 16002020 Need inquiry

Note

Earle's salts

Hanks' salts

MEM (alpha modification of Eagle’s MEM)Product Name Form Packaging Product Code Unit Price

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Liquid 500 mL 16002950 JPY 2,100

Note

MEM

DMEM/F-12 (1:1 Mixture of Dulbecco’s Modified Eagle’s Medium and Ham’s F-12)Product Name Form Packaging Product Code Unit Price Note

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Liquid 500 mL 16007100 JPY 2,000

DMEM/F-12

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Price List

Classical media

Basal Media EagleProduct Name Form Packaging Product Code Unit Price

Basal Media Eagle

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Liquid 500 mL 16001505 JPY 1,600 Liquid 500 mL 16001500 Need inquiry

Note

Earle's salts

Hanks' salts

Miscellaneous MediaProduct Name Form Packaging Product Code Unit Price

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IMDMwithout L-Glutamine

Liquid 500 mL 16007505 JPY 2,100

McCoy's 5A ModifiedMediumwithout L-Glutamine


Note

Media 199 Earle

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Price List

Sera and plasma

Animal seraProduct Name Form Packaging Product Code Unit PriceFetal Bovine Serum Liquid, frozen 100 mL 12180610 Need inquiryFetal Bovine Serum Liquid, frozen 500 mL 12180650 Need inquiryNewborn Calf Serum Liquid, frozen 100 mL 12181610 JPY 5,000Newborn Calf Serum Liquid, frozen 500 mL 12181650 JPY 20,000Calf Serum Liquid, frozen 100 mL 12182610 JPY 4,500Calf Serum Liquid, frozen 500 mL 12182650 JPY 18,000Bovine Serum Liquid, frozen 100 mL 12183610 JPY 4,000Bovine Serum Liquid, frozen 500 mL 12183650 JPY 16,000Equine Serum Liquid, frozen 100 mL 12180110 JPY 5,000Equine Serum Liquid, frozen 500 mL 12180150 JPY 20,000Porcine Serum Liquid, frozen 100 mL 12180910 JPY 5,000Porcine Serum Liquid, frozen 500 mL 12180950 JPY 20,000Goat Serum Liquid, frozen 100 mL 12180810 JPY 10,000Goat Serum Liquid, frozen 500 mL 12180850 JPY 40,000Sheep Serum Liquid, frozen 100 mL 12180210 JPY 8,000Sheep Serum Liquid, frozen 500 mL 12180250 JPY 32,000Rabbit Serum Liquid, frozen 100 mL 12180310 JPY 7,500Rabbit Serum Liquid, frozen 500 mL 12180350 JPY 30,000Chicken Serum Liquid, frozen 100 mL 12180510 JPY 40,000Chicken Serum Liquid, frozen 500 mL 12180550 JPY 160,000Mouse Serum Liquid, frozen 10 mL 12181001 JPY 15,000Mouse Serum Liquid, frozen 20 mL 12181002 JPY 20,000Mouse Serum Liquid, frozen 50 mL 12181005 JPY 40,000Rat Serum Liquid, frozen 10 mL 12181101 JPY 25,000Rat Serum Liquid, frozen 50 mL 12181105 JPY 65,000Guinea Pig Serum Liquid, frozen 10 mL 12180701 JPY 10,000Guinea Pig Serum Liquid, frozen 20 mL 12180702 JPY 18,000Guinea Pig Serum Liquid, frozen 50 mL 12180705 JPY 30,000

Animal plasmaProduct Name Form Packaging Product Code Unit Price AnticoagulantBovine Plasma Liquid, frozen 100 mL 12273610 JPY 20,000 CitrateEquine Plasma Liquid, frozen 100 mL 12170110 JPY 15,000 HeparinPorcine Plasma Liquid, frozen 100 mL 12170910 JPY 94,000 CitrateGoat Plasma Liquid, frozen 100 mL 12270110 JPY 18,000 CitrateSheep Plasma Liquid, frozen 100 mL 12270210 JPY 15,000 CitrateChicken Plasma Liquid, frozen 100 mL 12200090 JPY 45,000 CitrateRabbit Plasma Liquid, frozen 100 mL 12220310 JPY 41,000 HeparinRabbit Plasma Liquid, frozen 100 mL 12270310 JPY 39,000 CitrateCanine Plasma Liquid, frozen 50 mL 12225005 JPY 30,000 CitrateCanine Plasma Liquid, frozen 50 mL 12225305 JPY 32,000 HeparinBeagle Plasma Liquid, frozen 50 mL 12260415 JPY 60,000 HeparinBeagle Plasma Liquid, frozen 100 mL 12260410 JPY 110,000 HeparinRat Plasma Liquid, frozen 20 mL 12272103 JPY 45,000 HeparinRat Plasma Liquid, frozen 50 mL 12272105 JPY 80,000 HeparinGinea Pig Plasma Liquid, frozen 100 mL 12273310 JPY 150,000 Citrate

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Price List

Blood

Preserved animal bloodProduct Name Form Packaging Product Code Unit PriceEquine Blood, Preserved Blood 50 mL 12075105 JPY 2,500Equine Blood, Preserved Blood 100 mL 12070110 JPY 4,000Sheep Blood, Preserved Blood 50 mL 12075205 JPY 2,500Sheep Blood, Preserved Blood 100 mL 12070210 JPY 4,000Rabbit Blood, Preserved Blood 50 mL 12075305 JPY 10,000Rabbit Blood, Preserved Blood 100 mL 12070310 JPY 14,500Goose Blood, Preserved Blood 20 mL 12072402 JPY 12,000Goose Blood, Preserved Blood 50 mL 12075405 JPY 25,000Chiken Blood, Preserved Blood 50 mL 12070505 JPY 7,500Chiken Blood, Preserved Blood 100 mL 12070510 JPY 12,000Bovine Blood, Preserved Blood 50 mL 12075605 JPY 9,800Bovine Blood, Preserved Blood 100 mL 12070610 JPY 17,500Guinea Pig Blood, Preserved Blood 20 mL 12072702 JPY 10,500Guinea Pig Blood, Preserved Blood 50 mL 12075705 JPY 21,000Rat Blood, Preserved Blood 10 mL 12091201 JPY 10,000Rat Blood, Preserved Blood 50 mL 12091205 JPY 40,000Mouse Blood, Preserved Blood 10 mL 12071801 JPY 18,000African Green Monkey Blood,Preserved

Blood 10 mL 12081101 JPY 49,000

Defibrinated or hemolyzed animal bloodProduct Name Form Packaging Product Code Unit PriceEquine Blood, Defibrinated Blood 50 mL 12065105 JPY 2,500Equine Blood, Defibrinated Blood 100 mL 12060110 JPY 4,000Sheep Blood, Defibrinated Blood 50 mL 12065205 JPY 3,600Sheep Blood, Defibrinated Blood 100 mL 12060210 JPY 6,000Rabbit Blood, Defibrinated Blood 50 mL 12065305 JPY 13,000Rabbit Blood, Defibrinated Blood 100 mL 12060310 JPY 22,500Bovine Blood, Defibrinated Blood 50 mL 12065605 JPY 16,000Bovine Blood, Defibrinated Blood 100 mL 12060610 JPY 28,000Equine Blood, Hemolyzed Blood 100 mL 12090110 JPY 5,000

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Price List

All products derived from human blood are provided from the organizations that have obtained FDA approval for manufacture and distribution.They are for a laboratory research use only and not be able to use as raw materials to prepare diagnostic or therapeutic agents.

Sera, plasma, and blood

Normal human seraProduct Name Form Packaging Product Code Unit PricePooled Normal Human Serum Liquid, frozen 100 mL 12181201 JPY 24,000Pooled Normal Human Serum Liquid, frozen 500 mL 12181450 JPY 72,000Individual Normal Human Serum Liquid, frozen 100 mL 12181211 JPY 30,000Pooled Normal Human Serum,Type AB

Liquid, frozen 100 mL 12181301 JPY 40,000

Pooled Normal Human Serum,Defibrinated

Liquid, frozen 100 mL 12183320 Need inquiry

Pooled Normal Human Serum,Defibrinated


Individual Normal Human UmbilicalSerum


Individual Normal Human UmbilicalSerum


Pathologic human plasma and seraProduct Name Form Packaging Product Code Unit PriceIndividual HyperlipidemiｃHuman Plasma


Individual Syphilis-PositiveHuman Serum


Individual HBV-PositiveHuman Serum


Individual HIV-PositiveHuman Serum


Individual HCV-PositiveHuman Serum


Individual CRP-PositiveHuman Serum


Individual RF-PositiveHuman Serum


Individual ASO-PositiveHuman Serum


Individual CMV-PositiveHuman Serum


Normal human plasmaProduct Name Form Packaging Product Code Unit Price AnticoagulantPooled Normal Human Plasma Liquid, frozen 100 mL 12250110 JPY 10,000 CitratePooled Normal Human Plasma Liquid, frozen 100 mL 12250210 JPY 20,000 HeparinIndividual Normal Human Plasma Liquid, frozen 100 mL 12271210 JPY 15,000 CitrateIndividual Normal Human Plasma Liquid, frozen 100 mL 12271310 JPY 25,000 Heparin

Normal human blood

All products listed above are treated with a mixture of citrate, phosphate, and dextrose as an anticoagulant at the blood collection.

Product Name Form Packaging Product Code Unit PriceNormal Human Blood, Type A Blood 450 mL 12081445 JPY 70,000Normal Human Blood, Type B Blood 450 mL 12081545 JPY 70,000Normal Human Blood, Type O Blood 450 mL 12081645 JPY 70,000Normal Human Blood, Type AB Blood 450 mL 12082102 JPY 70,000

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Major Products

KBM 500 series developed for human T cell culture

KBM 500 series (501/502, 540, 550 and 560) are the most frequently used for adoptive immunotherapy in Japan.

KBM 500 series

Other domestic andoverseas products(ALyS, AIM-V, etc.)

【Features】･ All KBM media are prepared using

selected high quality reagents or raw materials including GMP-grade stuff.

･ They do not contain proteins other than serum albumin which is injection grade and recombinant human insulin.

･ Buffering ability is strengthened so that pH during culture is stable.

･ Kanamycin sulphate is formulated as an antibiotics.

･ Shelf life of each medium is comparatively longer even in the presence of L-glutamine.

･ The products are manufactured at our factory as certified ISO 9001 and ISO 13485.

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0 5 10 15 20 25 300

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Cytotoxicity of activated lymphocytesto K562 cells

KBM 500 SeriesKBM 500 SeriesLiquid media for activated human lymphocytes

KBM500 series is very popular with serum-free culture media in the fields of immunological and clinical researches in activation ofhuman T cells and cellular immune response.

are optimized for activation of KBM502and KBM501lymphocytes, particularly natural killer (NK) cells and cytotoxic T cells (CTL), and the subsequent expansion culture of the activated lymphocytes, respectively.

is optimized for activation and the subsequent KBM530expansion culture of human lymphocytes even in the absence of albumin.

was developed to support the growth of T cells activated KBM540with immobilized anti CD3 antibody (20 percents more proliferative than the growth achieved in KBM530)

was developed to support the growth of T cells activated KBM550with immobilized anti CD3 antibody. The growth promoting ability is almost the same as that of KBM540.

is the same as that of KBM550. KBM551The formulation of However, IL-2 is absent. Before use, add an appropriate concentration of IL-2 to the medium.

was developed for the activation and expansion culture KBM561of lymphocytes with NK or CTL activity. However, IL-2 is absent. Before use, add an appropriate concentration of IL-2 to the medium.

Major Products

0 2 4 6 8 10 12 1450

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Proliferative response of IL-2-dependent humanlymphocytes on immobilized anti CD3 antibody

KBM 561A (Company G)KBM 551

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Major Products

【KBM220 for MDCK monolayer culture】free culture of MDCK cells (dog -is suitable for serumKBM 220

kidney origin) for which the study of membrane permeability of mesenchymal cells in the academic field or for making viruses to manufacture vaccines in an industrial world.

origin protein derivatives with a -contains the plantKBM 220low molecular weight. It is relatively easy to purify the virus because the medium does not contain serum.

【KBM230 for Vero monolayer culture】Vero cells (African green monkey kidney origin) have been cultured everywhere and are probably the most contributed cells to prepare various vaccines all over the world.

was developed to culture the Vero cells. KBM230The medium contains the animal origin protein derivatives with a low molecular weight. It is relatively easy to purify virus because the medium does not contain serum.

can be used to culture MDCK cells and KBM230Moreover, BHK-21 cells (Syrian hamster kidney origin)

【KBM240 for CHO suspension culture】was developed for culturing CHO cells (Chinese KBM240

hamster ovary origin) which are introduced foreign genes to produce proteins such as a human type of antibodies and that of cytokines.

is optimized for the high density suspension culture KBM240of CHO cells, resulting in a high recombinant protein recovery. This medium contains the animal origin protein derivatives with a low molecular weight. It is relatively easy to purify therecombinant protein because the medium does not contain serum.

KBM200 SeriesKBM200 SeriesSerum-free liquid media for industrial purpose

【KBM270 for CHO monolayer culture】was developed for culturing CHO cells. KBM270is optimized for a monolayer culture of the KBM270

cells contains no animal-derived material. Thus, this medium is a “chemically defined“ synthetic medium, enabling purification of recombinant proteins.

Culture time (days)

The

num

ber o

f cel

ls (

10-4

cells

/mL)

Growth of CHO-K1 cells

KBM240C (Company G)

0 1 2 3 4 5 6 710

20

30

50

100

200

Growth of MDCK cells

0 1 2 3 4 5 60.50.7

1.0

2.0

3.0

5.07.0

10.0

20.0

The

num

ber o

f cel

ls (

10-4

cells

/cm

2 )Culture time (days)

KBM220M (Company G)

Growth of Vero cells

0 2 4 6 81

2

345

7

10

20

Culture time (days)

The

num

ber o

f cel

ls (

10-4

cells

/cm

2 )

KBM240V (Company G)

0 2 4 6 8

0.5

0.7

1.0

2.0

3.0

5.0

7.0

10.0

20.0

30.0

50.0

Culture time (days)

The

num

ber o

f cel

ls (×

10-4ce

lls/m

L)

Growth of CHO-K1cells

Ham's F-12 with 10% fetal bovine serumCD (Company H)KBM270

20

KBM300 Series KBM300 Series Serum-free liquid media for hybridoma culture

KBM 700 SeriesKBM 700 SeriesSerum-free liquid medium for insect cells

【KBM710 for insect cell culture】

KBM710 was developed for the recombinant protein expression in Sf-9 cells derived from Spodoptera frugiperda as well as promoting the cell growth. KBM710 contains proteins with a molecular weight of less than 500. Therefore, the medium is practically suitable for the expression of proteins with a larger molecular weight. KBM710 is able to apply for not only the shaking and stirring cultures but also the static culture.

【KBM300 for hybridoma culture】

density culture of established -was developed for achieving a highKBM300hybridoma cells and a high yield of antibody at more than 1 mg/mL.

nly is formulated with quite low content of proteins. It contains oKBM300transferrin and insulin at as little as 5 mg/ml each. It does not contain albumin.Excessive pH change is minimum due to the effect of a combination of HEPES and sodium bicarbonate.

cells are able to grow and secret antibody in hybridomaThus, a variety of . KBM300

【KBM306 for hybridoma culture】

as contains the same protein species (totally less than 10 mg/ml) KBM306KBM300 does. The shelf life is 8 months when preserved at 2–8C in the dark.The medium decreases cytotoxic activity caused by reactive oxygen species such as hydrogen peroxide. Excessive pH change is minimum due to the effect of a combination of a certain buffer agent and sodium hydrogen carbonate.Hybrid cells after cell fusion and during HAT selection are able to grow in this medium.It is commonly understood that cells cultured in a serum-containing medium require adaptation to shift to serum-free culture conditions by gradually decreasing the serum concentration until the cells adapted to the conditions.

does not need serum supplementation for the KBM306However, culture in adaptation.

Major Products

Sｆ9細胞に対する　培地性能比較試験

0

200

400

600

800

1000

1200

1400

0 2 4 6 8

培養時間　（日）

細胞数 (× 104　cells/mL)

ＫＢＭ710 Ａ社

Growth of Sf-9 cells

Culture time (days)

The

num

ber o

f cel

ls (

10-4

cells

/mL)

ＫＢＭ710

S medium (Company G)

21

Recommended Products

KM Banker and KM Banker KM Banker and KM Banker ⅡⅡSpecial cryopreservativesKM Banker

KM Banker is a chemically defined cryopreservative which contains neither serum components nor other proteins. Therefore, this cryopreservative is used when cells adapted to serum-free culture conditions are frozen for their preservation.Monolayer cells, lymphoid cells, and transformed cells are cryo-preserved without remarkable lose of viability. These cells include Vero cells, MDCK cells, CHO cells, HeLa cells and other cell lines in addition to activated lymphocytes.

ⅡKM Banker

KM Banker II was developed for cryopreservation of lymphoid cells.To improve the viability after cell-thawing, KBM Banker II is supplemented with human albumin. Particularly, cryopreservation of activated human lymphocytes is recommended.Other cells cultured in a suspension form are also cryopreserved in KM Banker II. These cells include Daudi cells, Raji cells, K562 cells, Molt-4F cells, and CTLL-2 cells.

Survival of human lymphocytes stored in variouscryopreservatives up to 1 year at -80C

0

20

40

60

80

100

1 week 1 month 2 months 6 months 1 year

Preservation Period

Servival (%) of thawed cells

KM Banker II

Company A

Company B

KM Banker

KM Banker II

C (Company N)

B (Company L)

KM Banker

Growth of activated human lymphocytes stored invarious cryopreservatives for 1 week at -80C

0

50

100

150

200

250

0 1 2 3 4 5 6 7

Days of culture

The number of cells (x 10-4 cells/mL)

KM Banker II

KM Banker

Company A

Company B

KM Banker II

KM Banker

C (Company N)

B (Company L)

Viability of human lymphocytes storedin various cryopreservatives for 1 week at -80C

0

20

40

60

80

100

Company A Company B KM Banker KM Banker II

Survival (%) of thawed cells

C(Company N)

B(Company L)

KM Banker KM Banker II

Human lymphocytes stored in variouscryopreservatives for 1 month at -80C

0

20

40

60

80

100

KM Banker Company A Company B


KM Banker C(Company N)

B(Company L)

Molt-4F cells stored in variouscryopreservatives for 6 months at -80C

0

20

40

60

80

100



KM Banker B(Company L)

C(Company N)

Vero cells stored in variouscryopreservatives for 6 months at -80C

0

20

40

60

80

100



B(Company L)


MDCK cells stored in variouscryopreservatives for 1 month at -80C

0

20

40

60

80

100




B(Company L)

NoticeNoticeKM Bankers contain dimethyl sulfoxide (DMSO) as a

cryoprotective agent. DMSO will erode plastic resin such as polyethylene terephthalate (PET) with lapse of time.

A cryopreservative fluid is packaged in glass bottles, guaranteeing the quality of the products.

The products are always kept at 2-8C in the dark up to 2 years after the production. Freezing-and-thawing of the cryopreservatives is prohibited.

22

A typical monolayer of human umbilical venous endothelial cells are shown in above photograph.The tube contains umbilical venous endothelial cells, obtained from a healthy donor, within three passages of culture.The number of the cells are 1 x 106 cells in each tube. The existence of HIV, HBV, HCV, mycoplasma, Treponema pallidum, fungous, or bacteria is negative.The ability of cell growth is guaranteed up to 15 divisions after the products have been distributed.Purity of the cells has been confirmed by the presence of factor Ⅷand cellular labeling with Dil-Ac-LD. The data sheet describing race, sex, and age of the donor will be attached.The tube is frozen in a box filled with dry ice while delivering. It should be preserved at below -80C until cell-thawing and subsequent culture.

Normal Human Umbilical Venous Endothelial Normal Human Umbilical Venous Endothelial CellsCells

This product is composed of a 500-mL basal medium (RPMI 1640) supplemented with 10% fetal bovine serum (FBS) and an additive.

Quality of FBS was carefully checked and only the batch of FBS passed a growth examination has been supplemented to the medium.

The additive is a mixture of epidermal growth factor and endothelial cell growth supplement at concentrations of 10 g/L and 70 g/L, respectively, when added to the medium.

The shelf life of both components is one year and half if they are preserved at -20C.

Please use the complete medium within three weeks after adding the extract to the basal medium and stored at 2-8C in the dark.

Medium for Normal Human Vascular EndothelialMedium for Normal Human Vascular EndothelialCellsCells

Miscellaneous Products

A tube contains the additive consisted of epidermal growth factor and endothelial cell growth supplement.

23

This product is composed of a 500-mL serum-free basal medium and a pituitary gland extract derived from New Zealand bovines. The animals are proven not to suffer from bovine spongiform encephalopathy.The shelf life of both components is one year and half if they are preserved at -20C.

Please use the complete medium within three weeks after adding the extract to the basal medium and stored at 2-8C in the dark.

SerumSerum--Free Medium for Normal Human Dermal Free Medium for Normal Human Dermal KeratinocytesKeratinocytes

Miscellaneous Products

Cell dissociation reagents (2 smaller square bottles) seen in a photograph above are available as an option.A tube in front of bottles is another product. It contains normal human dermal keratinocytes.

Normal Human Dermal Normal Human Dermal KeratinocytesKeratinocytes

A typical monolayer of human dermal keratinocytes are shown in above photograph. The tube contains dermal keratinocytes, obtained from a healthy donor, within three passages of culture.The number of the cells are 1 x 106 cells in each tube. The existence of HIV, HBV, HCV, mycoplasma, Treponema pallidum, fungous, or bacteria is negative.The ability of cell growth is guaranteed up to 15 divisions after the products have been distributed.When the cells are plated in a 25-cm2 flask, they grow to confluence in about five days of culture.The data sheet describing race, sex, and age of the donor will be attached.The tubel is frozen in a box filled with dry ice while delivering. It should be preserved at below -80C until cell-thawing and subsequent culture.

24


In Vitro Fertilization and Embryo Culture Kits In Vitro Fertilization and Embryo Culture Kits ““ARTECARTEC””

Kohjin Bio’s in vitro fertilization and embryo culture kits (ARTEC) were developedas a serum-free culture system optimized for in vitro culture of human embryos up to a bastcyst stage.The kits consist of basal culture medium, serum substitutes, nutritional supplements, and external gas environment stabilizer. These are essential as nutrients, antioxidants, and pH-buffering agents to support of the growth of embryos at earlier stages.

Form: LiquidStorage: 2-8C in the dark (AH-25, C1-25, and F-500)

-20C (B-25, C2-25, D-25, and E-25)Shelf life: 1 Year

Prior to use ARTEC-PRI and ARTC-SEC are prepared by mixing components 1) to 3) and 1) to 5), respectively.

ARTEC-PRI for culture of embryos at an early stage within 3 days of fertilization1) AH-25: Basal embryo culture medium (25 mL)2) B-25: Additive to the basal medium (0.3 mL)3) C1-25: Serum substitute used at 2- to 8-cell stages (4.7 mL)

ARTEC-SEC for culture of embryos at 8-cell to blastcyst stages 1) AH-25: Basal embryo culture medium (25 mL)2) B-25: Additive to the basal medium (0.3 mL)3) C2-25: Serum substitute used at 8-cell to blastcyst stages (4.2 mL)4) D-25: Supplement used at 8-cell to blastcyst stage (0.3 mL)5) E-25: Supplement used at 8-cell to blastcyst stage (0.2 mL)

ARTEC F-500 for cultures of embryos at 2-cell to blastcyst stagesExternal fluid to supply nutrients and to stabilize gas environment (500 mL)

SerumSerum--Free Medium for Mouse Embryonic Stem Free Medium for Mouse Embryonic Stem CellsCells

Kohjin Bio’s embryonic stem cell culture medium was developed as a serum-free medium optimized for the culture of mouse embryonic stem cells.This medium is a ready-to-use liquid medium. However, addition of leukemia inhibitory factor to the medium is essentially required.

Form: LiquidPackaging: 250 mL and 500 mLStorage: -20CShelf life: 1 Year

【Specifications】Osmolality 285-315 mOsm/kg, determined by freezing point depression osmometrypH

Aseptic test

Endotoxin test

Mycoplasma test

Growth test

Differentiation test

Sufficient growth promotion when representative mouse embrionic stem cellsare cultured as compared to the culture in control mediumUnder the appropriate differentiation-stimulatory conditions, sufficientpromotion of differentiation when representative mouse embrionic stem cellsare cultured as compared to the culture in control medium

7.3-7.5, determined using a glass electrode

Less than 0.3 EU/mL by Limulus amebocyte lysate assay

Negative by Mycotrim® TC (Irvine Scientific) assay

Negative by TGC and SCD cultures

25

R. Dulbecco and M. Vogt improved usual physiologic saline solutions and composed a new phosphate-buffered saline, which is referred to as Dulbecco's Phosphate-Buffered Saline (D-PBS), in 1957. D-PBS contains potassium chloride, calcium chloride, and magnesium sulfate in addition to common potassium-sodium phosphate salts and sodium chloride. Because of the osmolarity, ion concentration, and a pH-buffering range are similar to those of mammals. D-PBS is used when tissues, organs, or cells are processed for culture or treated for biochemical research. In particular, D-PBS lacking calcium chloride, and magnesium sulfate is widely used when a single cell suspension is prepared by dissociating cells from organs or cultured monolayers after tryptic digestion.

J. Hanks improved usual balanced salt solutions and composed a new one, which is referred to as Hanks' Balanced Salt Solution (HBSS), in 1976. HBSS is frequently used to maintain cellular metabolism during transportation or manipulation of tissues, organs, or cells.

Kohjin Bio Co. manufactures and distributes physiologically isotonic buffer solutions listed in a table on the right. These solutions are prepared by dissolving only quality raw materials in GMP-grade purified water.

Physiologically Isotonic Buffer SolutionsDulbecco’s Phosphate-Buffered Saline (D-PBS)Hanks’ Balanced Salt Solution (HBSS)


*1 Determined by freezing point depression osmometry.*2 The solution is diluted 10 times with purified water.*3 Determined using a glass electrode.*4 Determined after 14-day cultures in TGC and SCD media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific) assay.

D-PBS(-)without Ca2+ and Mg2+

10-fold ConcentratedD-PBS(-)

HBSS(-)without Ca2+ and Mg2+

HBSS(+)with Ca2+ and Mg2+

without Phenol Red16005550 16005510 16229015 16230020

140.0100.0100.0

200 2000 400.0 400.0200 2000 60.0 60.0

8000 80000 8000.0 8000.0350.0 350.0

1150 11500 47.9 47.9

1000.0 1000.010.0

Clear red solution Clear solution265-295 265-295*2

7.1-7.7 7.1-7.7*2 7.1-7.5

Calcium chloride (anhydrous)Magnesium chloride hexahydrateMagnesium sulfate heptahydrate

Potassium phosphate monobasicSodium chlorideSodium hydrogen carbonateSodium phosphate dibasic (anhydrous)Other compounds:

D-Glucose

Membrane filtration (0.2 m)

Liquid in a square PET bottle500 mL

Product Name

Product CodeFormPackaging

265-295

15‒25°C

Inorganic salts:

Potassium chloride

2 YearsComponents Concentration （mg/L）

StorageShelf Life

Phenol red

Clear solutionAppearanceSpecifications

Aseptic Test*4

Endotoxin Test*5

Mycoplasma Test*6 Negative

Osmolality*1

pH*3

SterilizationNegativeLess than 0.1 EU/mL

26

BME Basal Media EagleEagle’s Basal Media

H. Eagle develpoed Basal Medium Eagle (BME) in 1955 for culturing HeLa cells and mouse L cells. BME was in the forefront of synthetic cell culture media, e.g. Eagle's Minimum Essential Medium. Many investigators have extended BME and its modifications to other cells in culture such as human diploid WI-38 cells.

Kohjin Bio Co. manufactures and distributes four types of BME listed in a table on the right. These media are prepared by dissolving only quality raw materials in GMP-grade purified water.

Major Products

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3 For the determination the media are exposed to

humidified air containing 5% CO2 at 37C overnight.*4 Determined after 14-day cultures in TGC and SCD

media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific)

assay.*7 The media are supplemented with 5% fetal bovine

serum and with or without L-glutamine at the indicated concentration. Thereafter, Vero cells are assessed for their growth.

BME Earle withoutL-Glutamine

BME Earle withL-Glutamine

BME Hanks withoutL-Glutamine

BME Hanks withL-Glutamine

16001495 16001490 16001505 16001500

200.00 200.00 140.00 140.0097.67 97.67 97.67 97.67

400.00 400.00 400.00 400.0060.00 60.00

6800.00 6800.00 8000.00 8000.002200.00 2200.00 350.00 350.00

47.88 47.88121.74 121.74

21.00 21.00 21.00 21.0012.00 12.00 12.00 12.00

292.00 292.0010.81 10.81 10.81 10.8126.00 26.00 26.00 26.0026.00 26.00 26.00 26.0036.47 36.47 36.47 36.477.50 7.50 7.50 7.50

16.50 16.50 16.50 16.5024.00 24.00 24.00 24.004.00 4.00 4.00 4.00

18.04 18.04 18.04 18.0423.50 23.50 23.50 23.50

1.00 1.00 1.00 1.001.00 1.00 1.00 1.001.00 1.00 1.00 1.001.00 1.00 1.00 1.002.00 2.00 2.00 2.001.00 1.00 1.00 1.001.00 1.00 1.00 1.000.10 0.10 0.10 0.101.00 1.00 1.00 1.00

1000.00 1000.00 1000.00 1000.0010.00 10.00 10.00 10.00

Other compounds:

Clear orange to red solution270-305

Hemicalcium D-pantothenate

Thiamine hydrochloride

D-Glucose

AppearanceSpecifications

6.9-7.4270-310

Sterilization Membrane filtration (0.2 m)pH*2Osmolality*1

L-Phenylalanine

Choline chlorideFolic acid

L-TryptophanL-Threonine

L-Tyrosine

Vitamins:

Inorganic salts:

L-CystineL-Arginine hydrochloride

Shelf Life

Amino acids:

Sodium chloride

Concentration （mg/L）9 Months

L-Histidine hydrochloride monohydrateL-Glutamine

Sodium phosphate monobasic (anhydrous)

Sodium hydrogen carbonate

500 mL2‒8°C in the dark

PackagingForm

Storage

L-Isoleucine

RiboflavinPyridoxal hydrochlorideNiacinamide

L-Lysine hydrochloride

L-Valine

L-Methionine

myo -Inositol

D-Biotin

L-Leucine

Aseptic Test*4 NegativeLess than 0.3 EU/mLNegativeSufficient growth promotion when compared to the growth in each control medium

Sodium phosphate dibasic (anhydrous)

7.0-7.5*3

Phenol red

Cell growth Test*7Mycoplasma Test*6Endotoxin Test*5

Potassium phosphate monobasic

Calcium chloride (anhydrous)Magnesium sulfate (anhydrous)Potassium chloride

Components

Product Code

Product Name

Liquid in a square PET bottle

27

Major Products

MEMEagle's Minimum Essential MediaMinimum Essential Media Eagle

H. Eagle developed the synthetic medium composed of minimum nutritional requirements for culturing mouse L cells in 1959. The medium, which is referred to as Minimum Essential Medium (MEM), is an advanced medium derived from Basal Medium Eagle. Nowadays, MEM is widely used to culture many species of mammalian monolayer cells.

Kohjin Bio Co. manufactures and distributes four types of MEM listed in a table on the right. These media are prepared by dissolving only quality raw materials in GMP-grade purified water.

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3 For the determination the media are exposed to

humidified air containing 5% CO2 at 37°C overnight.*4 Determined after 14-day cultures in TGC and SCD

media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific) assay.*7 The media are supplemented with 5% fetal bovine

serum and with or without L-glutamine at the indicated concentration. Thereafter, Vero cells are assessed for their growth.

MEM Earlewithout L-Glutamine

MEM Earlewith L-Glutamine

MEM Hankswithout L-Glutamine

MEM Hankswith L-glutamine

16002005 16002000 16002025 16002020

200.00 200.00 140.00 140.0098.00 98.00 98.00 98.00

400.00 400.00 400.00 400.0060.00 60.00

6800.00 6800.00 8000.00 8000.002200.00 2200.00 350.00 350.00

47.88 47.88121.74 121.74

126.00 126.00 126.00 126.0023.78 23.78 23.78 23.78

292.00 292.0042.00 42.00 42.00 42.0052.00 52.00 52.00 52.0052.00 52.00 52.00 52.0073.00 73.00 73.00 73.0015.00 15.00 15.00 15.0032.00 32.00 32.00 32.0048.00 48.00 48.00 48.0010.00 10.00 10.00 10.0036.00 36.00 36.00 36.0046.00 46.00 46.00 46.00

1.00 1.00 1.00 1.001.00 1.00 1.00 1.001.00 1.00 1.00 1.002.00 2.00 2.00 2.001.00 1.00 1.00 1.001.00 1.00 1.00 1.000.10 0.10 0.10 0.101.00 1.00 1.00 1.00

1000.00 1000.00 1000.00 1000.0010.00 10.00 10.00 10.00

Inorganic salts:Components

Calcium chloride (anhydrous)

Amino acids:

Potassium chlorideMagnesium sulfate (anhydrous)

Concentration （mg/L）

Liquid in a square PET bottle500 mL2‒8°C in the dark9 Months

NegativeSufficient growth promotion when compared to the growth in each control mediumCell growth Test*7

Mycoplasma Test*6

Thiamine hydrochlorideOther compounds:

D-GlucosePhenol red

NegativeLess than 0.3 EU/mL


L-Arginine hydrochloride

Potassium phosphate monobasic

Sodium phosphate dibasic (anhydrous)

Sodium chlorideSodium hydrogen carbonate

Sodium phosphate monobasic (anhydrous)

L-ThreonineL-Tryptophan

L-Cystine

L-LeucineL-Lysine hydrochlorideL-Methionine

L-Histidine hydrochloride monohydrateL-Isoleucine

L-Glutamine

L-Phenylalanine

L-ValineL-Tyrosine

Niacinamide

Vitamins:

Choline chlorideFolic acid

myo -InositolHemicalcium D-pantothenate

Appearance

Pyridoxal hydrochlorideRiboflavin

SpecificationsClear orange to red solution

Product Name

Shelf LifeStoragePackagingFormProduct Code

Endotoxin Test*5Aseptic Test*4

270-3106.9-7.5*3 6.9-7.4pH*2

Osmolality*1

Sterilization

275-315

28

Major Products

RPMI 1640

G. E. Moore and his co-workers developed RPMI 1640 medium for culturing leukemia cell lines at Roswell Park Memorial Institute in 1966. RPMI 1640 medium is one of the useful synthetic media for suspension culture of lymphoid cells, holding an established position with advance in cellular immunological research including lymphocyte response to certain plant lectins.

Kohjin Bio Co. manufactures and distributes two types of RPMI 1640 listed in tables on the right. Both media are prepared by dissolving only quality raw materials in GMP-grade purified water.

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3 For the determination the media are exposed to humidified air containing 5% CO2 at 37°C

overnight.*4 Determined after 14-day cultures in TGC and SCD media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific) assay.*7 The media are supplemented with 5% fetal bovine serum and with or without L-glutamine

at the indicated concentration. Thereafter, Molt-4F cells are assessed for their growth.

Product Name RPMI 1640 without L-Glutamine RPMI 1640 with L-Glutamine

Product Code 16005005 16005050FormPackagingStorageShelf LifeComposition

Calcium nitrate tetrahydrate 100.000 100.000Magnesium sulfate (anhydrous) 48.840 48.840Potassium chloride 400.000 400.000Sodium chloride 6000.000 6000.000Sodium hydrogen carbonate 2000.000 2000.000Sodium phosphate dibasic (anhydrous) 800.000 800.000

L-Arginine 200.000 200.000L-Asparagine monohydrate 56.820 56.820L-Asparatic acid 20.000 20.000L-Cystine 49.840 49.840L-Glutamic acid 20.000 20.000L-Glutamine 300.000Glycine 10.000 10.000L-Histidine 15.000 15.000Hydroxy-L-proline 20.000 20.000L-Isoleucine 50.000 50.000L-Leucine 50.000 50.000L-Lysine hydrochloride 40.000 40.000L-Methionine 15.000 15.000L-Phenylalanine 15.000 15.000L-Proline 20.000 20.000L-Serine 30.000 30.000L-Threonine 20.000 20.000L-Tryptophan 5.000 5.000L-Tyrosine 20.000 20.000L-Valine 20.000 20.000

4-Aminobenzoic acid 1.000 1.000D-Biotin 0.200 0.200Choline chloride 3.000 3.000Cyanocobalamin 0.005 0.005Folic acid 1.000 1.000Hemicalcium D-pantothenate 0.250 0.250myo -Inositol 35.000 35.000Niacinamide 1.000 1.000Pyridoxine hydrochloride 1.000 1.000Riboflavin 0.200 0.200Thiamine hydrochloride 1.000 1.000



Amino acids:

Inorganic salts:

Vitamins:

D-Glucose 2000.000 2000.000Glutathione (reduced) 1.000 1.000Phenol red 5.000 5.000

AppearanceOsmolality*1

pH*2

SterilizationAseptic Test*4

Endotoxin Test*5

Mycoplasma Test*6

Cell growth Test*7

Clear orange to red solution

Negative

NegativeLess than 0.3 EU/mL

270-310

Other compounds:

Sufficient growth promotion when compared to the growth in eachcontrol medium

6.9-7.5*3


Specifications

Product code 16005005 16005050Composition Concentration （mg/L）

29

Ham's F-12 F-12 Nutrient Mixtures

R. G. Ham developed F-12 Nutrient Mixture (Ham's F-12) in 1965 to achieve clonal growth of CHO cells, HeLa cells, and mouse L cells. Ham's F-12 has been extended to culture a variety of cell lines. Furthermore, the medium is used as a basal medium of modified synthetic media by others.

Kohjin Bio Co. manufactures and distributes two types of Ham's F-12 listed in tables on the right. Both media are prepared by dissolving only quality raw materials in GMP-grade purified water.

Major Products

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3 For the determination the media are exposed to humidified air containing 5%

CO2 at 37°C overnight.*4 Determined after 14-day cultures in TGC and SCD media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific) assay.*7 The media are supplemented with 5% fetal bovine serum and with or without L-

glutamine at the indicated concentration. Thereafter, CHO-K1 cells are assessed for their growth.

1802.0000 1802.00004.0810 4.08100.0800 0.08000.2100 0.21001.2000 1.20000.1600 0.1600

110.0000 110.00000.7000 0.7000

Specifications

SterilizationpH*2Osmolality*1

Appearance


Clear ligh red to light pink solution285-3157.0-7.6*3

Cell growth Test*7

Mycoplasma Test*6Endotoxin Test*5Aseptic Test*4

Sufficient growth promotion when compared to the growth ineach control medium

Less than 0.3 EU/mLNegative

Negative

D-Glucose

Phenol red

Thymidine

HypoxanthineLineleic acid

Sodium pyruvate

Other compounds:

Putrescine dihydrochloride

DL--Lipoic acid

Ham's F-12 without L-Glutamine Ham's F-12 with L-Glutamine

16006205 16006250

33.2200 33.220057.2200 57.2200

223.6000 223.60007599.0000 7599.00001176.0000 1176.0000142.0000 142.0000

8.9000 8.9000211.0000 211.000015.0000 15.000013.0000 13.000035.0000 35.000014.7000 14.7000

146.00007.5000 7.5000

21.0000 21.00004.0000 4.0000

13.0000 13.000036.5000 36.50004.5000 4.50005.0000 5.0000

34.5000 34.500010.5000 10.500012.0000 12.00002.0000 2.00005.3700 5.3700

11.7000 11.7000

0.0073 0.007314.0000 14.00001.4000 1.40001.3000 1.30000.5000 0.5000

18.0000 18.00000.0400 0.04000.0600 0.06000.0400 0.04000.3000 0.3000

0.0025 0.00250.8300 0.83000.8600 0.8600

9 MonthsComposition Concentration （mg/L）Inorganic salts:

Calcium chloride (anhydrous)Magnesium chloride (anhydrous)Potassium chloride

Liquid in a square PET bottle500 mL2‒8°C in the dark

FormProduct Code

Product Name

L-Arginine hydrochlorideL-Asparagine monohydrateL-Asparatic acidL-Cysteine hydrochloride monohydrateL-Glutamic acidL-GlutamineGlycineL-Histidine hydrochloride monohydrateL-IsoleucineL-LeucineL-Lysine hydrochlorideL-MethionineL-PhenylalanineL-ProlineL-Serine

Vitamins:

D-BiotinCholine chlorideCyanocobalamin

Zinc sulfate heptahydrate

Riboflavin

L-ThreonineL-TryptophanL-TyrosineL-Valine

Folic acid

myo -InositolNiacinamide

Hemicalcium D-pantothenate

Trace elements:

Copper(II) sulfate pentahydrateIron(II) sulfate heptahydrate

Pyridoxine hydrochloride


Shelf LifeStoragePakaging

L-Alanine

Sodium chlorideSodium hydrogen carbonateSodium phosphate dibasic (anhydrous)Amino acids:

16006205 16006250Product codeComposition Concentration （mg/L）

30

Alpha MEM (MEM)Alpha Modification of Eagle's Minimum Essential Media

Major Products

C. P. Stanners and his co-workers modified Minimum Essential Medium (MEM) to extend for culturing mouse-hamster hybrid cells in 1971. The modified MEM is referred to as MEM. MEM is supplemented with additives that are not formulated to original MEM. These include deoxyribonucleosides, ribonucleosides, non-essential amino acids, and certain vitamins. MEM is also used to culture cells in a dihydrofolate reductase(DHFR) selection system.

Kohjin Bio Co. manufactures and distributes two types of MEM listed in tables on the right. However, both media contains neither deoxyribonucleosidesnor ribonucleosides. Cells lacking DHFR-producing gene will fail to grow in these media even when L-glutamine is supplemented. Both media are prepared by dissolving only quality raw materials in GMP-grade purified water.

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3 For the determination the media are exposed to humidified air containing 5% CO2 at

37°C overnight.*4 Determined after 14-day cultures in TGC and SCD media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific) assay.*7 The media are supplemented with 5% fetal bovine serum and with or without L-

glutamine at the indicated concentration. Thereafter, Vero cells are assessed for their growth.

D-Glucose 1000.00 1000.00DL--Lipoic acid 0.20 0.20Phenol red 10.00 10.00Sodium pyruvate 110.00 110.00


pH*2


Endotoxin Test*5

Mycoplasma Test*6

Cell growth Test*7

Other compounds:

Less than 0.3 EU/mLNegativeSufficient growth promotion when compared to the growth ineach control medium

7.1-7.7*3

Negative

Clear red solution285-315


Specifications


Product NameMEM without L-Glutamineand Nucleic Acid-RelatedCompounds

MEM without Nucleic Acid-Related Compounds


Calcium chloride (anhydrous) 200.00 200.00Magnesium sulfate (anhydrous) 98.00 98.00Potassium chloride 400.00 400.00Sodium chloride 6800.00 6800.00Sodium hydrogen carbonate 2200.00 2200.00Sodium phosphate monobasic (anhydrous) 121.73 121.73

L-Alanine 25.00 25.00L-Arginine hydrochloride 127.00 127.00L-Asparagine monohydrate 50.00 50.00L-Asparatic acid 30.00 30.00L-Cysteine hydrochloride monohydrate 100.00 100.00L-Cystine 23.78 23.78L-Glutamic acid 75.00 75.00L-Glutamine 292.00Glycine 50.00 50.00L-Histidine hydrochloride monohydrate 42.00 42.00L-Isoleucine 52.00 52.00L-Leucine 52.00 52.00L-Lysine hydrochloride 73.00 73.00L-Methionine 15.00 15.00L-Phenylalanine 32.00 32.00L-Proline 40.00 40.00L-Serine 25.00 25.00L-Threonine 48.00 48.00L-Tryptophan 10.00 10.00L-Tyrosine 35.79 35.79L-Valine 46.00 46.00

D-Biotin 0.10 0.10Choline chloride 1.00 1.00Cyanocobalamin 1.40 1.40Folic acid 1.00 1.00Hemicalcium D-pantothenate 1.00 1.00myo -Inositol 2.00 2.00Niacinamide 1.00 1.00Pyridoxal hydrochloride 1.00 1.00Riboflavin 0.10 0.10Sodium L-ascorbate 50.00 50.00Thiamine hydrochloride 1.00 1.00



Vitamins:

Amino acids:

Inorganic salts:

31

DMEM Dulbecco's Modified Eagle's Media

R. Dulbecco and G. Freeman modified Basal Medium Eagle (BME) for culturing mouse embryonic cells in 1959. The modified BME, which is referred to as Dulbecco's Modified Eagle's Medium (DMEM), characteristically contains a 4-fold concentration of amino acids and vitamins. Furthermore, DMEM enriched with glucose from 1000 mg/L to 4500 mg/L is widely used for not only a conventional culture but also a large-scale high density culture of many cell lines.

Kohjin Bio Co. manufactures and distributes four types of DMEM listed in a table on the right. These media are prepared bydissolving only quality raw materials in GMP-grade purified water.

Major Products

*1 Determined by freezing point depression osmometry.

*2 Determined using a glass electrode.*3 For the determination the media are

exposed to humidified air containing 5% CO2 at 37°C overnight.

*4 Determined after 14-day cultures in TGC and SCD media.

*5 Determined by Limulus amebocyte lysate assay.

*6 Determined by MycoTrim® TC (Irvine Scientific) assay.

*7 The media are supplemented with 5% fetal bovine serum and with or without L-glutamine at the indicated concentration. Thereafter, Vero cells are assessed for their growth.

Product Name DMEM (Low Glucose)without L-Glutamine

DMEM (Low Glucose)with L-Glutamine

DMEM (High Glucose)without L-Glutamine

DMEM (High Glucose)with L-Glutamine

Product Code 16003005 16003550 16003505 16003560FormPackagingStorageShelf LifeComposition

Calcium chloride (anhydrous) 200.00 200.00 200.00 200.00Magnesium sulfate (anhydrous) 97.67 97.67 97.67 97.67Iron(Ⅲ) nitrate nonahydrate 0.10 0.10 0.10 0.10Potassium chloride 400.00 400.00 400.00 400.00Sodium chloride 6400.00 6400.00 6400.00 6400.00Sodium hydrogen carbonate 3700.00 3700.00 3700.00 3700.00Sodium phosphate monobasic (anhydrous) 108.70 108.70 108.70 108.70

L-Arginine hydrochloride 84.00 84.00 84.00 84.00L-Cystine 48.34 48.34 48.34 48.34L-Glutamine 584.00 584.00Glycine 30.00 30.00 30.00 30.00L-Histidine hydrochloride monohydrate 42.00 42.00 42.00 42.00L-Isoleucine 105.00 105.00 105.00 105.00L-Leucine 105.00 105.00 105.00 105.00L-Lysine hydrochloride 146.00 146.00 146.00 146.00L-Methionine 30.00 30.00 30.00 30.00L-Phenylalanine 66.00 66.00 66.00 66.00L-Serine 42.00 42.00 42.00 42.00L-Threonine 95.00 95.00 95.00 95.00L-Tryptophan 16.00 16.00 16.00 16.00L-Tyrosine 71.59 71.59 71.59 71.59L-Valine 94.00 94.00 94.00 94.00

Choline chloride 4.00 4.00 4.00 4.00Folic acid 4.00 4.00 4.00 4.00Hemicalcium D-pantothenate 4.00 4.00 4.00 4.00myo -Inositol 7.20 7.20 7.20 7.20Niacinamide 4.00 4.00 4.00 4.00Pyridoxal hydrochloride 4.00 4.00 4.00 4.00Riboflavin 0.40 0.40 0.40 0.40Thiamine hydrochloride 4.00 4.00 4.00 4.00

D-Glucose 1000.00 1000.00 4500.00 4500.00Phenol red 15.00 15.00 15.00 15.00Sodium pyruvate 110.00 110.00


pH*2


Endotoxin Test*5

Mycoplasma Test*6

Cell growth Test*7

SpecificationsClear red solution

Membrane filtration (0.2 m)Negative

7.0-7.6*3320-350

Sufficient growth promotion when compared to the growth in each control medium




Other compounds:

Vitamins:

Amino acids:

Inorganic salts:

32

Major Products

Media 199 Earle

J. F. Morgan and his co-workers developed Medium 199 in 1950 to study nutritional requirements for culture of chick embryonic fibroblasts. Medium 199 characteristically contains certain vitamins, nucleic acid-related compounds, sugars, and antioxidants in addition to common amino acids and vitamins. The medium is frequently used as a basal cell culture medium for virus production.

Kohjin Bio Co. manufactures and distributes two types of Media 199 with Earle’s salts listed in tables on the right. Both media are prepared by dissolving only quality raw materials in GMP-grade purified water.

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3 For the determination the medium is exposed to humidified air containing 5% CO2 at

37°C overnight.*4 Determined after 14-day cultures in TGC and SCD media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific) assay.*7 The medium is supplemented with 5% fetal bovine serum and with or without L-

glutamine at the indicated concentration. Thereafter, Vero cells are assessed for their growth.


Product Name Medium 199 Earlewithout L-Glutamine

Medium 199 Earlewith L-Glutamine


Calcium chloride (anhydrous) 200.000 200.000Iron(Ⅲ) nitrate nonahydrate 0.720 0.720Magnesium sulfate (anhydrous) 97.670 97.670Potassium chloride 400.000 400.000Sodium chloride 6800.000 6800.000Sodium hydrogen carbonate 2200.000 2200.000Sodium phosphate monobasic (anhydrous) 121.740 121.740

L-Alanine 25.000 25.000L-Arginine hydrochloride 70.000 70.000L-Asparatic acid 30.000 30.000L-Cysteine hydrochloride monohydrate 0.110 0.110L-Cystine 19.950 19.950L-Glutamic acid 75.000 75.000L-Glutamine 100.000Glycine 50.000 50.000L-Histidine hydrochloride monohydrate 21.880 21.880Hydroxy-L-proline 10.000 10.000L-Isoleucine 20.000 20.000L-Leucine 60.000 60.000L-Lysine hydrochloride 70.000 70.000L-Methionine 15.000 15.000L-Phenylalanine 25.000 25.000L-Proline 40.000 40.000L-Serine 25.000 25.000L-Threonine 30.000 30.000L-Tryptophan 10.000 10.000L-Tyrosine 40.000 40.000L-Valine 25.000 25.000

4-Aminobenzoic acid 0.050 0.050D-Biotin 0.010 0.010Calciferol 0.100 0.100Choline chloride 0.500 0.500Folic acid 0.010 0.010Hemicalcium D-pantothenate 0.010 0.010myo -Inositol 0.050 0.050Menadione 0.010 0.010Niacin 0.025 0.025Niacinamide 0.025 0.025Pyridoxal hydrochloride 0.025 0.025Pyridoxine hydrochloride 0.025 0.025Retinol acetate 0.140 0.140Riboflavin 0.010 0.010Sodium L-ascorbate 0.056 0.056Thiamine hydrochloride 0.010 0.010DL--Tocopherol phosphate disodium salt 0.010 0.010

Vitamins:

Inorganic salts:

Amino acids:



2-Deoxy-D-ribose 0.500 0.500D-Glucose 1000.000 1000.000D-Ribose 0.500 0.500

Adenine hemisulfate 10.000 10.000Adenosine 5'-monophosphate sodium salt 0.239 0.239Adenosine 5'-triphosphate disodium salt 1.000 1.000Guanine hydrochloride 0.300 0.300Hypoxanthine 0.300 0.300Thymine 0.300 0.300Uracil 0.300 0.300Xanthine sodium salt 0.340 0.340

Cholesterol 0.200 0.200Glutathione (reduced) 0.050 0.050Phenol red 20.000 20.000Tween 80 20.000 20.000Sodium acetate (anhydrous) 50.000 50.000


pH*2


Endotoxin Test*5

Mycoplasma Test*6

Cell growth Test*7

Other compounds:


Clear orange to red solutionSpecifications

Sugars:

Nucleic acid-related compounds:

Sufficient growth promotion when compared to the growth ineach control medium

270-3107.0-7.6*3

Membrane filtration (0.2 m)Negative

33

McCoy's 5A Modified Medium without L-Glutamine

T. A. McCoy and his co-workers modified Basal Medium 5A in 1959 for culturing Novikoff rat hepatoma cells. The modified medium is referred to as McCoy's 5A Modified Medium. McCoy's 5A Modified Medium contains animal organ extract in addition to common amino acids and vitamins. The medium has been extended to culture other types of cells derived from a variety of organs such as bone marrow, kidney, lung, and spleen.

Kohjin Bio Co. manufactures and distributes McCoy's 5A Medium without L-glutamine in tables on the right. This medium is prepared by dissolving only quality raw materials in GMP-grade purified water.

Major Products

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3For the determination the medium is exposed to humidified air containing 5%

CO2 at 37°C overnight.*4 Determined after 14-day cultures in TGC and SCD media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific) assay.*7 The medium is supplemented with 5% fetal bovine serum and 219.2 mg/L

L-glutamine. Thereafter, Vero cells are assessed for their growth.

Product Name McCoy's 5A Modified Medium without L-Glutamine

Product Code 16008005Form Liquid in a square PET bottlePackaging 500 mLStorage 2‒8°C in the darkShelf Life 9 MonthsComposition Concentration （mg/L）

Calcium chloride (anhydrous) 100.00Magnesium chloride (anhydrous) 97.67Potassium chloride 400.00Sodium chloride 6460.00Sodium hydrogen carbonate 2200.00Sodium phosphate monobasic (anhydrous) 504.30

L-Alanine 13.36L-Arginine hydrochloride 42.14L-Asparagine monohydrate 45.03L-Asparatic acid 19.97L-Cysteine hydrochloride monohydrate 35.14L-Glutamic acid 22.07Glycine 7.51L-Histidine hydrochloride monohydrate 20.96Hydroxy-L-proline 19.67L-Isoleucine 39.36L-Leucine 39.36L-Lysine hydrochloride 36.54L-Methionine 14.92L-Phenylalanine 16.52L-Proline 17.27L-Serine 26.28L-Threonine 17.87L-Tryptophan 3.06L-Tyrosine 18.12L-Valine 17.57

4-Aminobenzoic acid 1.00D-Biotin 0.20Choline chloride 5.00Cyanocobalamin 2.00Folic acid 10.00Hemicalcium D-pantothenate 0.20myo -Inositol 36.00Niacin 0.50Niacinamide 0.50Pyridoxal hydrochloride 0.50Pyridoxine hydrochloride 0.50Riboflavin 0.20Sodium L-ascorbate 0.56Thiamine hydrochloride 0.20

Inorganic salts:

Vitamins:

Amino acids:

Bacto peptone (Difco) 600.00D-Glucose 3000.00Glutathione (reduced) 0.50Phenol red 10.00

Appearance Clear orange to red solutionOsmolality*1 270-310pH*2 6.9-7.5*3

Sterilization Membrane filtration (0.2 m)Aseptic Test*4 NegativeEndotoxin Test*5 Less than 0.3 EU/mLMycoplasma Test*6 Negative

Cell growth Test*7Sufficient growth promotion when compared to thegrowth in control medium

Specifications

Other compounds:Composition Concentration （mg/L）

34

DMEM/F-121:1 Mixture of Dulbecco’s Modified Eagle’s Medium and Ham’s F-12

It has been thought that serum is an essential cell growth-promoting factor in culture. Since 1979 G. Sato and his co-workers have postulated a new concept that molecules replaceable serum in mammalian cell culture are considered as hormones where the term "hormone“ does not mean a classical endocrine hormone but is a growth factor. Representative hormones include serum albumin, transferrin, insulin, polypeptides growth factors, amine compounds, and trace elements. Several combinations of these hormones at appropriate concentrations support growth of many types of cells in serum-free medium. A 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F-12 (DMEM/F-12) has been widely used as a representative serum-free basal medium.

Kohjin Bio Co. manufactures and distributes two types of DMEM/F-12 (1:1) listed in tables on the right. Both media are prepared by dissolving only quality raw materials in GMP-grade purified water.

Major Products

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3 For the determination the media are exposed to humidified air

containing 5% CO2 at 37°C overnight.*4 Determined after 14-day cultures in TGC and SCD media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific) assay.*7 The media are supplemented with 5% fetal bovine serum and with or

without L-glutamine at the indicated concentration. Thereafter, Vero cells are assessed for their growth.


Product Name DMEM/F-12without L-Glutamine

DMEM/F-12with L-Glutamine


Calcium chloride (anhydrous) 116.6000 116.6000Magnesium chloride (anhydrous) 28.6400 28.6400Magnesium sulfate (anhydrous) 48.8400 48.8400Potassium chloride 311.8000 311.8000Sodium chloride 6995.5000 6995.5000Sodium hydrogen carbonate 2438.0000 2438.0000Sodium phosphate dibasic (anhydrous) 71.0200 71.0200Sodium phosphate monobasic (anhydrous) 54.3400 54.3400

L-Alanine 4.4500 4.4500L-Arginine hydrochloride 147.5000 147.5000L-Asparagine monohydrate 7.5000 7.5000L-Asparatic acid 6.6500 6.6500L-Cysteine hydrochloride monohydrate 17.5600 17.5600L-Cystine 24.0050 24.0050L-Glutamic acid 7.3500 7.3500L-Glutamine 365.0000Glycine 18.7500 18.7500L-Histidine hydrochloride monohydrate 31.4800 31.4800L-Isoleucine 54.4700 54.4700L-Leucine 59.0500 59.0500L-Lysine hydrochloride 91.2500 91.2500L-Methionine 17.2400 17.2400L-Phenylalanine 35.4800 35.4800L-Proline 17.2500 17.2500L-Serine 26.2500 26.2500L-Threonine 53.4500 53.4500L-Tryptophan 9.0200 9.0200L-Tyrosine 38.6900 38.6900L-Valine 52.8500 52.8500

D-Biotin 0.0035 0.0035Choline chloride 8.9800 8.9800Cyanocobalamin 0.6800 0.6800Folic acid 2.6500 2.6500Hemicalcium D-pantothenate 2.2400 2.2400myo -Inositol 12.6000 12.6000Niacinamide 2.0200 2.0200Pyridoxine hydrochloride 2.0310 2.0310Riboflavin 0.2190 0.2190Thiamine hydrochloride 2.1700 2.1700

Vitamins:

Amino acids:

Inorganic salts:

9 MonthsConcentration （mg/L）

Liquid in a square PET bottle500 mL2‒8°C in the dark

Copper(II) sulfate pentahydrate 0.0013 0.0013Iron(II) sulfate heptahydrate 0.4170 0.4170Iron(Ⅲ) nitrate nonahydrate 0.0500 0.0500Zinc sulfate heptahydrate 0.4320 0.4320

D-Glucose 3151.0000 3151.0000Hypoxanthine 2.0445 2.0445Lineleic acid 0.0420 0.0420DL--Lipoic acid 0.1050 0.1050Phenol red 8.1000 8.1000Putrescine dihydrochloride 0.0810 0.0810Sodium pyruvate 55.0000 55.0000Thymidine 0.3650 0.3650


pH*2


Endotoxin Test*5

Mycoplasma Test*6

Cell growth Test*7

Trace elements:


Other compounds:

Specifications

Sufficient growth promotion when compared to the growth ineach control MEM

Clear orange to red solution295-3257.1-7.7*3

NegativeMembrane filtration (0.2 m)

35

IMDM without L-GlutamineIscove's Modified Dulbecco's Medium without L-Glutamine

L. Guilbert and N. Iscove modified Dulbecco's Modified Eagle's Medium (DME) in 1976 for culturing B cells, T cells, and hybrid cells in the presence of appropriate stimulators. The modified medium contains potassium nitrate, selenium, sodium pyruvate, and HEPES in addition to common amino acid and vitamins formulated to DME. This medium is referred to as Iscove's Modified Dulbecco's Medium (IMDM). IMDM has been widely used as a defined basal serum-free medium particularly when high-density cell culture is required.

Kohjin Bio Co. manufactures and distributes IMDM without L-glutamine listed in tables on the right. Glutamine synthetase (GS) selection is often used by culturing mammalian cells such as CHO cells to express recombinant proteins. This selection system is based on which the cells transfected the GS gene can grow in glutamine-free medium. IMDM without L-glutamine is prepared by dissolving only quality raw materials in GMP-grade purified water.

Major Products

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3 For the determination the medium is exposed to humidified air

containing 5% CO2 at 37°C overnight.*4 Determined after 14-day cultures in TGC and SCD media.*5 Determined by Limulus amebocyte lysate assay.*6 Determined by MycoTrim® TC (Irvine Scientific) assay.*7 The medium is supplemented with 5% fetal bovine serum and 584 mg/L

L-glutamine. Thereafter, Molt-4F cells are assessed for their growth.

Product Name IMDMwithout L-Glutamine

Product Code 16007505Form Liquid in a square PET bottlePackaging 500 mLStorage 2‒8°C in the darkShelf Life 9 MonthsComposition Concentration （mg/L）

Calcium chloride (anhydrous) 165.000Magnesium sulfate (anhydrous) 97.670Potassium chloride 330.000Potassium nitrate 0.076Sodium chloride 4505.000Sodium hydrogen carbonate 3024.000Sodium phosphate monobasic (anhydrous) 108.700Sodium selenite 0.017

L-Alanine 25.000L-Arginine hydrochloride 84.000L-Asparagine monohydrate 28.400L-Asparatic acid 30.000L-Cystine 70.000L-Glutamic acid 75.000Glycine 30.000L-Histidine hydrochloride monohydrate 42.000L-Isoleucine 105.000L-Leucine 105.000L-Lysine hydrochloride 146.000L-Methionine 30.000L-Phenylalanine 66.000L-Proline 40.000L-Serine 42.000L-Threonine 95.000L-Tryptophan 16.000L-Tyrosine 72.010L-Valine 94.000

D-Biotin 0.013Choline chloride 4.000Cyanocobalamin 0.013Folic acid 4.000Hemicalcium D-pantothenate 4.000myo -Inositol 7.200Niacinamide 4.000Pyridoxal hydrochloride 4.000Riboflavin 0.400Thiamine hydrochloride 4.000

Vitamins:

Amino acids:

Inorganic salts:

D-Glucose 4500.000HEPES 5958.000Phenol red 15.000Sodium pyruvate 110.000

Appearance Clear orange to red solutionOsmolality*1 275-315pH*2 6.9-7.4*3

Sterilization Membrane filtration (0.2 m)Aseptic Test*4 NegativeEndotoxin Test*5 Less than 0.3 EU/mLMycoplasma Test*6 Negative

Cell growth Test*7Sufficient growth promotion when compared to thegrowth in control medium

Specifications

Other compounds:Composition Concentration （mg/L）

36

Major Products

MEM Essential Amino Acid Solution (50-fold Concentrate) and MEM Non-Essential Amino Acid Solution (100-fold Concentrate) are composed of amino acids formulated to alpha modification of Eagle's Minimum Essential Medium (MEM) at 50-fold and 100-fold, respectively, concentrations. These concentrated solutions are used when MEM is prepared using individual raw materials or added to media as supplemental amino acids depending on the extent of nutritional exhaustion during cell culture. MEM Vitamin Solution (100-fold Concentrate) is composed of vitamins formulated to Eagle's Minimum Essential Medium (MEM) at a 100-fold concentration. This concentrated solution is used when MEM or MEM is prepared using individual raw materials or added to media as supplemental vitamins depending on the extent of nutritional exhaustion during cell culture.

Kohjin Bio Co. manufactures and distributes the concentrated solutions listed in tables on the right. These concentrates are prepared by dissolving only quality raw materials in GMP-grade purified water.

*1 Determined by freezing point depression osmometry.*2 Determined using a glass electrode.*3 Determined after 14-day cultures in TGC and SCD media.*4 Determined by Limulus amebocyte lysate assay.*5 Determined by MycoTrim® TC (Irvine Scientific) assay.*6 MEM is reconstituted with a 2% volume of the MEM essential amino acid solution and other

medium components. The reconstituted medium is further supplemented with 5% fetal bovine serum. Thereafter, Vero cells are assessed for their growth.

*7 Eagle’s MEM is supplemented with a 1% volume of the MEM non-essential amino acid solution and 5% fetal bovine serum. Thereafter, HeLa cells are assessed for their growth.

*8 Eagle’s MEM is reconstituted with a 1% volume of the MEM vitamin solution and other medium components. The reconstituted medium is further supplemented with 5% fetal bovine serum. Thereafter, Vero cells are assessed for their growth.

50-fold ConcentratedMEM Essential Amino Acid Solution

Product Name

Product CodeFormPackagingStorageShelf LifeComposition Concentration （mg/L）

632012002100262026203625755

16502380510

18002340


pH*2


Endotoxin Test*4

Mycoplasma Test*5

L-CystineL-Histidine hydrochloride monohydrateL-IsoleucineL-Leucine

400-5000.7-1.9Membrane filtration (0.2 µm)

Clear solution

Cell growth Test*6Sufficient growth promotion when compared tothe growth in control medium

100 mL

MEM essential amino acid solution(50-fold concentrate)

16223004Liquid in a square PET bottle

L-Arginine hydrochloride

‒20°C2 Years

Amino acids:

L-Lysine hydrochlorideL-MethionineL-PhenylalanineL-ThreonineL-TryptophanL-TyrosineL-ValineSpecifications

NegativeLess than 0.3 EU/mLNegative

100-fold ConcentratedMEM Non-Essential Amino Acid Solution

Product Name


8901500

1330

1470

750

1150

1050


pH*2


Endotoxin Test*4

Mycoplasma Test*5


Clear solution50-902.5-4.0

NegativeLess than 0.3 EU/mLNegative


L-Asparagine monohydrateL-Asparatic acidL-Glutamic acidGlycine

L-SerineL-Proline

Specifications

100 mL‒20°C2 Years

Amino acids:

L-Alanine

MEM Non-Essential Amino Acid Solutionwithout L-Glutamine (100-fold Concentrate)


100-fold ConcentratedMEM Vitamin Solution

Product Name


10010010020010010010

100


pH*2


Endotoxin Test*4

Mycoplasma Test*5


Membrane filtration (0.2 m)NegativeLess than 0.3 EU/mLNegative

Clear yellow solution5-155.0-70

Specifications

Choline chloride

100 mL

2 Years


Pyridoxal hydrochlorideRiboflavin

MEM Vitamin Solution (100-fold Concentrate)


‒20°C

Vitamins:

Folic acidHemicalcium D-pantothenatemyo -InositolNiacinamide

37

TeraScanTeraScan VPCVPCMicrofluorocytometer for Cytotoxicity Test

51Cr release assay has been so far widely used to evaluate cytotoxicity in immunology, pharmacology, toxicology and other medical and biological fields. However, the use of isotopes involves a variety of limitations. Radioisotopes must be treated in registered radioisotope facilities and used within their half-life. Furthermore, their quantity to be deposited or used at one time as well as the ways of safekeeping and waste disposal must be followed the governmental or local radioisotope regulations.

TeraScan is a microfluorocytometer developed for measuring cytotoxicity using a standard routine technique without limitations stated above. TeraScan consists of three pieces of apparatus, i.e. an inverted epifluorescent microscope, a control unit, and a data processor.

TeraScan assay requires fluorescent dye as a cytosolic marker of target cells, such as Calcein-AM. The routine procedure of the fluorometry is much simplified. Separation of the cells and incubation medium is not necessary after the target cells contact the effector cells, enabling kinetic analysis.

Not only NK cell- or CTL-mediated cytotoxicity but also antibody-dependent cellular cytotoxicity can be assessed by the TeraScan microfluorocytometer.

Outside Product

Manufacturer:Minerva Tech K. K.Minerva Tech K. K., Tokyo, JapanTEL: +81-3-5458-8865FAX: +81-3-5458-0390

Domestic distributor:KohjinKohjin Bio Co., Ltd.Bio Co., Ltd., Sakado, JapanTEL: +81- 49-284-3781FAX: +81- 49-284-4784

K562 cells

y = 0.9484x + 9.832R2 = 0.9639n = 24

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Daudi cells

y = 1.1557x - 11.128R2 = 0.877n = 24

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　　　　　　　 Fluorescent equipment Incident fluorescent illuminator mounted on Zeiss 　 inverted epifluorescent microscope　　　　　　　 Objective lens Plan Neofluar 10/0.30　　　　　　　 Detector Photomultiplier　　　　　　　 Filter set Excitation filter (485/20)

Dichroic filter (FT510)Barrier filter (BP515-565)

　　　　　　　 Wavelength range 400 – 800 nm　　　　　　　 Object holders 96-Well microplate

Terasaki plate（option）Slide glass (option）

　　　　　　　 Stage Computer-aided X-Y movement (110 mm 70 mm)　　　　　　　 Light source Halogen lamp　(12 V, 100 W) 　　　　　　　 Power consumption AC 100/200 V (50/60 Hz), 500 W　　　　　　　 Dimensions 370 (W) 700 (D) 540 (H) mm (microscope)

150 (W) 365 (D) 320 (H) mm (control unit)　　　　　　　 Weight 26 kg (microscope)

6 kg (control unit)　　　　　　　 Hardware MPU 1.5 GHz, RAM 512 MB　　　　　　　 Operating system Windows 2000/XP　　　　　　　 Softwares VPC for measurement

CalCt for calculation and analysis

【Specifications】

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Approach on Microbiology

Products used in clinical or food microbiology

Prepared Plate Media/Prepared Tube Media

*Media for microbiological culture

*Media for drug resistant bacteria detection

*Media for pathogenic bacteria detection

*Media for environmental monitoring

*Media for food microbiology

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Microbiological Media

“KBM“, the raw material of the microorganism media is used Laboratorious CONDA S.A (Madrid and Spain). The CONDA company provides high quality products to more than 80 countries such as Europe, Latin America, Asia, the Middle East, and Africa. In Japan, we business tie-up by our company and receives the support of the processing technology, the quality control technique, and the product development technology, and is supplying high-quality and a steady life medium to Japan.

Product Name Packaging List Pr ice

”ＫＢＭ” ＴＣＢＳ Agar 20 JPY 2 ,800

”ＫＢＭ” ＤＨＬ Agar 20 JPY 2 ,800

”ＫＢＭ” CT-SMAC Agar 20 JPY 2 ,800

”ＫＢＭ” ＳＳ Agar 20 JPY 2 ,800

”ＫＢＭ” Macconkey Agar 20 JPY 2 ,800

”ＫＢＭ” ＢＴＢ Agar 20 JPY 2 ,400

”ＫＢＭ” Muel le r-Hinton Agar 20 JPY 2 ,800

”ＫＢＭ” Muel le r-Hinton Agar with sheep blood 20 JPY 3 ,600

”ＫＢＭ” Muel le r-Hinton Chocolate Agar 20 JPY 3 ,600

”ＫＢＭ” Candida Chrom Agar 20 JPY 3 ,800

”ＫＢＭ” STEC Chrom Agar 20 JPY 5 ,000

”ＫＢＭ” SS/STEC Chrom Agar 20 JPY 5 ,000

”ＫＢＭ” Choco late Agar with Bac it rac in 20 JPY 4 ,000

”ＫＢＭ” ＧＣ Choco late Agar 20 JPY 3 ,600

”ＫＢＭ” HB Choco late Agar 20 JPY 3 ,600

”ＫＢＭ” Skir row's Agar 20 JPY 4 ,000

”ＫＢＭ” Modified Campylobacter Blood Agar 20 JPY 5 ,000

Product Name Packaging List Pr ice

”ＫＢＭ” Columbia ＣＡ Blood Agar 20 JPY 3 ,800

”ＫＢＭ” Columbia ＣＡ Blood Agar／Macconkey Agar 20 JPY 4 ,000

”ＫＢＭ” Sheep Blood Agar 20 JPY 3 ,600

”ＫＢＭ” ＮＡＤ Sheep Blood Agar 20 JPY 3 ,800

”ＫＢＭ” NAD Sheep Blood Agar/Macconkey Agar 20 JPY 4 ,000

”ＫＢＭ” Mann ito l Salt Agar Ⅱ with Egg Yo lk 20 JPY 3 ,600

”ＫＢＭ” Anaerobic Rabbit Blood Agar １x１０ JPY 4 ,500

”ＫＢＭ” Anaerobic Rabbit Blood Agar ５x４ JPY 9 ,000

”ＫＢＭ” Anaerobic Rabbit Blood Agar ／ＢＢＥ Agar １x１０ JPY 4 ,500

”ＫＢＭ” SS/ CT-SMAC Agar 20 JPY 3 ,600

”ＫＢＭ” Potato Dextrose Agar with Ch loramphen ico l 20 JPY 3 ,000

”ＫＢＭ” ＮＧＫＧ Agar 20 JPY 3 ,600

”ＫＢＭ” ＳＣＤ Agar 20 JPY 2 ,400

”ＫＢＭ” Standard Methods Agar 20 JPY 2 ,400

”ＫＢＭ” Nutr ient Agar 20 JPY 2 ,800

”ＫＢＭ” Sabouraud Dextrose Agar 20 JPY 3 ,000

【Agar media 】

Please inquire the products are not shown on the list and the custom-designed media are available.

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Main products

”ＫＢＭ” Sheep Blood Agar ”ＫＢＭ” ＧＣ Chocolate Agar ”ＫＢＭ” ＢＴＢ Agar

”ＫＢＭ” SS/ CT-SMAC Agar ”ＫＢＭ” Candida Chrom Agar

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"KBM" Anaerobic Rabbit Blood Agar

【Purpose of use】

"KBM" Anaerobic Rabbit Blood Agar is used to culture the obligate anaerobe from a variety clinical specimens. This medium is excellent in the growthperformance of anaerobic cocci, such as Genus, Bacteroides that high detection rate, Clostridium, and Peptostreptococcus that phastidious organisms. Moreover, it is easy to make a differentiation and optimum for isolation and cultivation of phastidious anaerobic bacteria from the clinical specimen for Gram-negative rods (black pigment producing bacteria) such as Porphyromonas and Prevotella.

【Composition】

（1 liter of purified water）

Casein Peptone ・・・・・・・・・10.0gAnimal tissue – Pepsin digestive peptone ・・5.0gYeast extract・・・・・・・・・・・・5.0gCorn starch ・・・・・・・5.0gSodium chloride・・・・・・・・・5.0gHeart infusion ・・・・・・・3.0gSodium sulfite ・・・・・・・・・0.1gL‐Cysteine hydrochloride・・・・・・・0.3gDipotassium hydrophosphate・・・・2.5gSodium thioglycolate ・・・・0.3gＬ‐arginine ・・・・・・・・・・1.0gSodium pyruvate ・・・・・・・1.0gVitamin K1 ・・・・・・・・・・・・10mgHemin ・・・・・・・・・・・・・・10mgAgar ・・・・・・・・・・・・・・・13.5gRabbit blood ・・・・・・・・・・・・25mlRabbit hemolysis blood ・・25ml

pH7.3±0.2

【 Feature 】

1. Growth performance of phastidious anaerobic bacteria with is phastidious

2. Adsorption of poisonous substance against anaerobic bacteria

3. Make a differentiation of black pigmentproducing bacteria

4. Formation of typical colony

5. Anaerobic wrapping package (Singleplate package)

P.melaninogenica

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"KBM" STEC Chromo Agar

【Purpose of use】

“KBM” STEC chromo agar is used for the Selective Isolation and Cultivation of E.coli O157, O26, and O111 that is Shigella Toxin producing Escherichia Coli (STEC) from a variety clinical specimens and non-clinical specimens.

【Composition】

（1 liter per purified water）

Gelatin digestive peptone ・・・・・・17.0gCasein digestive peptone ・・・・・・・

1.5gAnimal tissue digestive peptone・・・1.5gSTEC chromogenic substrate ・・・・・

7.5gSodium chloride ・・・・・・・・・・

5.0gBile salt ・・・・・・・・・・・・・

1.5gNeutral red ・・・・・・・・・・・・・

0.03gPotassium tellurite ・・・・・・・2.5mgCrystal purple ・・・・・・・・・・・

1.0mgCefixime ・・・・・・・・・・・

0.05mgAgar ・・・・・・・・・・・・・・・

13.5gpH8.1±0.2

【 Feature 】

1. Potassium tellurite with high pH (pH8.1) is used as the selective medicine of STEC. Due to the selectivity is higher than conventional selective media, the growth of the other bacteria are inhibited.

2. "KBM" STEC chromo agar will make the colony forming because of enzyme substrate mix.

E.coli O157 transparent to gray colorE.coli O26 blue – green colorE.coli O111 red purple color

It is able to be differentiation for O157, O26, and O111 on the same plate.

E.coli O157

E.coli O26

E.coli O111

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【 Test tube media 】

Product Name Unit List Price（Screw Cap）List Pr ice（PP Cap）

”KBM” TSI Agar 50 JPY 5,000 JPY 4,000

”KBM” LIM Agar 50 JPY 5,000 JPY 4,000

”KBM” SIM Medium 50 ‐ JPY 4,000

”KBM” VP Medium 50 ‐ JPY 4,000

”KBM” Simmons Citrate Sodium Agar 50 ‐ JPY 4,000

”KBM” Selenite Broth 50 JPY 8,000 ‐

”KBM” Anaerobic Semisolid Agar 50 JPY 12,000 ‐

Please inquire the products are not shown on the list and the custom-designed media are available.

Test tube media

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Antibody trust service

Feature of monoclonal antibody trust

The case of standard work for heterologous protein antigen

・The mono-antibody production hybridoma will be delivered in three months.

・The low price has been achieved. ・There is no cost claim if the monoclonal antibody

production is failed.

It has been succeeded to be made a line and save labor cost from the long experience and results, and to be going to make the monoclonal antibody surely in a short term.

Content Lead Time Basic RateMonoc lonal ant ibody t rust 11weeks JPY 900,000/Anti genMonoc lonal ant ibody for asc ites 4weeks JPY 20,000/Clone

Monoc lonal ant ibody c reat ion 2weeks JPY 60,000/Clone

Opt ionⅠ Lead Time Basic RatePept ide synthesis（ inc lude creat ion） 4weeks JPY 90 ,000/Ant igen Hapten ic（KLH, OVA） 1week JPY 80 ,000/Clone

Opt ion Ⅱ Lead Time Basic RateAssay method other than ELISA ‐ Inqu iryImmunoblot t ing ‐ JPY 100 ,000

Opt ion Ⅲ Lead Time Basic RateAsc ites 4weeks JPY 20 ,000/CloneMass ce l l cu lture 4weeks JPY 50 ,000/CloneRefin ing 2weeks JPY 60 ,000/Clone

Monoclonal antibody trust

Polyclonal antibody trustAnimals Numbers Lead Time Basic Rate

Rabbit 2 13weeks JPY 160 ,000

Mouse 5 13weeks JPY 150 ,000

Rat 3 13weeks JPY 150 ,000

Goat 1 13weeks JPY 200 ,000

Sheep 1 13weeks JPY 200 ,000

Rooster 1 13weeks JPY 80 ,000

ELISA x 1, trial collecting blood x 1, and collecting blood x 1 are contained. Please inquire the other animals that may accepted.

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Automated Microbial Detection System （Versa TREK）

Versa TREK is a fully-automatic blood culture diagnostic device being offered by TREK Diagnostic Co., Ltd (United States), and Kohjin bio has the license to import and sales.500 or more systems are currently operating in the world.

Advantage of Versa TREK･The only system with important graph analysis･The only system authorized by FDA for the test of all fluids including blood and other body

fluid. (CSF etc.)

Versa TREK is a device that detects the change in the gas pressure in the bottle head space. The gas consumption and production of the bacteria are reinforced by high nutrition media.There are only two bottles that are aerobic and anaerobic to be able to detect all types of bacteria. A special bottle media do not need any special additive at all. In addition, the tornado stir method, that is a new technology is built in an aerobic bottle so that oxygen may always become abundant while it cultures.

The clinical microorganism detection can obtain the performance, efficiency, and the cost reduction by using the Versa TREK system.

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A joint research with Bio-Theta, Ltd. (invested by Daikin Industries, Ltd.)　Conpany HP http://www.bio-theta.co.jp/

Cell with electrode

“DOX” is an innovative test system using an oxygen electrode method. With this revolutionary approach, anyone can easily detect and determine the number of hygiene-indicating bacteria; total number of General microorganisms, coliforms, and Escherichia coli. If the case of 105CFU/g of live bacteria, it is reduced by 6 hours which it conventional test method takes 24 hours or more.

In the test system that the measuring, judges, and records of the viable cell count from the breath amount of the bacteria automatically. The result of a measurement turns out from the specimen which has more fungi one by one. The test time and cost can be reduced by this system. The content of an automatic record can be used as a data base. Data is attempted, and the root cause analysis can be easily done by retrieving the condition, and the speedup and the labor saving can attempt feedback to the site.

Livestock farming, fishery, agriculture, prepared daily food manufacturing, flour milling, noodle manufacturing, confectionery, food-service operations, up to and including chemical production......many entities in a wide range of industries both at home and abroad are employing this state-of-the-art system.

Special Media (developed by Kohjin bio)

Rapid and Easy Food Bacteria Count System （DOX）

Hardware（DOX-60F）

Software

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Cosmetics

Product HP http://claudechauchard.com/

Dr. Claude Chauchard Extreme Youth LinesCosmetics by Cosmetics by KohjinKohjin bio bio

【Skin toning lotion】

It is a skin toner that mixed 18 kinds of amino acids after the research of nutritional content for the skin cells culture for years.

It is included that a botanical additionally hyaluronic acid and the vitamin group, and neither the surface-active agent nor perfume are used, the effect can be expected by using it for a long term.

When it is used after taking a bath, you will notice that the skin will be different in the next day for sure. Please use it for any scene as a base of the make-up.

JPY 6,800 / 100ml

-Also The liquid foundation (JPY 6,800 / 30ml) and The enriching gel cream (JPY 6,800 / 35g) are available

It has been found that the most effective element for the skin cells and those combinations by the research of the nutrient liquid.

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