Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG...

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Kloning av DNA fragment in i E.coli

Transcript of Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG...

Page 1: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC

Kloning av DNA fragment in i E.coli

Page 2: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 3: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 4: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 5: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 6: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 7: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 8: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 9: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 10: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 11: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC
Page 12: Kloning av DNA fragment in i Ecoli - Linköping University · PDF fileGGATCC BamHl CC TAGG GAATTC EcoRl CT TAAG GGCC Haelll CCGG GCGC Hhal CGCG CTCGAG Xhol GAGCTC

Gene DC andDX - pnas.org Okb 10, 20 30 40 50 I 5'UT/SS e4tTM/3'UT SUTTM,2 h 51UT/SS * DCs DCOD I tI S. I,-I, I Ia I A1 ... 5'UT/SS.1.tTM/3UT 3'UT/TM PtP 1 5UT/SS DX 0DX BamHl I CIOI

Gene DC andDX - pnas.org Okb 10, 20 30 40 50 I 5'UT/SS e4tTM/3'UT SUTTM,2 h 51UT/SS * DCs DCOD I tI S. I,-I, I Ia I A1 ... 5'UT/SS.1.tTM/3UT 3'UT/TM PtP 1 5UT/SS DX 0DX BamHl I CIOI

Dieses Dokument wurde von einer Papierkopie gescannt und ... · BMT/3/2 page 5 1 8 9 enzymes have been discovered, for example EcoRI recognises and cuts the sequence GAATTC. Figure

Dieses Dokument wurde von einer Papierkopie gescannt und ... · BMT/3/2 page 5 1 8 9 enzymes have been discovered, for example EcoRI recognises and cuts the sequence GAATTC. Figure

Microbe08n03 454 - APS Home · 2018. 12. 4. · 91 L Y Y G A D p X G G A V E S G V R F F A S p T C H H A p D gtC t 121 V Y S G V E S ... Sp = Sphl, and X = Xhol. B shows the nucleotide

Microbe08n03 454 - APS Home · 2018. 12. 4. · 91 L Y Y G A D p X G G A V E S G V R F F A S p T C H H A p D gtC t 121 V Y S G V E S ... Sp = Sphl, and X = Xhol. B shows the nucleotide

Palindrome - 國立臺灣大學juang.bst.ntu.edu.tw/files BC/BC2007/NA(1) 2007A.pdf · 2016. 2. 16. · Palindrome, Restriction Enzyme, Sticky Ends GAATTC GAATTC G AATTC G AATTC Sticky

Palindrome - 國立臺灣大學juang.bst.ntu.edu.tw/files BC/BC2007/NA(1) 2007A.pdf · 2016. 2. 16. · Palindrome, Restriction Enzyme, Sticky Ends GAATTC GAATTC G AATTC G AATTC Sticky

JA - Soluciones - DNA recombinante · Tecnología del DNA Recombinante La enzima de restricción EcoRI reconoce y se une a la secuencia palindrómica GAATTC. El corte del DNA en este

JA - Soluciones - DNA recombinante · Tecnología del DNA Recombinante La enzima de restricción EcoRI reconoce y se une a la secuencia palindrómica GAATTC. El corte del DNA en este

€¦ · rbs Sacl BamHI Spe I ... 3418 3095 3663 3563 1179 Accl Acelll Acil Alul Alwl Alw211 Alw441 AlwNl Apol Aval Avail BamHl Banl

€¦ · rbs Sacl BamHI Spe I ... 3418 3095 3663 3563 1179 Accl Acelll Acil Alul Alwl Alw211 Alw441 AlwNl Apol Aval Avail BamHl Banl

dnaconda.riken.jp2.0 Erectrophoresis 100 Restriction enzyme Noti Xhol Noti+Xhol O z o X 1 x TAE Buffer kbp kbp kbp kbp 19 nanogram DNA per lane ; 1% agarose gel , Expected size of

dnaconda.riken.jp2.0 Erectrophoresis 100 Restriction enzyme Noti Xhol Noti+Xhol O z o X 1 x TAE Buffer kbp kbp kbp kbp 19 nanogram DNA per lane ; 1% agarose gel , Expected size of

06_20_cancer_age.jpg. Gene Mutation: Origins and Repair Processes GAATTC GTATTC A a Skin cancers from Xeroderma pigmentosum, a recessive hereditary.

06_20_cancer_age.jpg. Gene Mutation: Origins and Repair Processes GAATTC GTATTC A a Skin cancers from Xeroderma pigmentosum, a recessive hereditary.

Effectof enterotoxin 1 in - Gut · Fasano, Noriega, Liao, Wang, Levine Xmnl Scal 2526- Xmnl Scal 3618. T7 promoter Kpal Apal Xhol Sall (Hincil) Accl Hindill EcoRV EcoRI-Pstl Smal

Effectof enterotoxin 1 in - Gut · Fasano, Noriega, Liao, Wang, Levine Xmnl Scal 2526- Xmnl Scal 3618. T7 promoter Kpal Apal Xhol Sall (Hincil) Accl Hindill EcoRV EcoRI-Pstl Smal

CHAPTER 8: RECOMBINANT DNA TECHNOLOGY · EcoRI 5’-GAATTC-3’ 3’-CTTAAG-5’ BamHI 5’-GGATCC-3’ 3’-CCTAGG-5’ SmaI 5’-GGGCCC-3’ 3’-CCCGGG-5’ The list and explanation

CHAPTER 8: RECOMBINANT DNA TECHNOLOGY · EcoRI 5’-GAATTC-3’ 3’-CTTAAG-5’ BamHI 5’-GGATCC-3’ 3’-CCTAGG-5’ SmaI 5’-GGGCCC-3’ 3’-CCCGGG-5’ The list and explanation

2 DASAR TEKNOLOGI DNA REKOMBINANPada gambar dibawah dapat dilihat misalnya enzim restriksi EcoRI,memotong molekul DNA pada urutan heksa-nukleotida 5’—GAATTC—3’ pada posisi

2 DASAR TEKNOLOGI DNA REKOMBINANPada gambar dibawah dapat dilihat misalnya enzim restriksi EcoRI,memotong molekul DNA pada urutan heksa-nukleotida 5’—GAATTC—3’ pada posisi

Genetic Engineering. Restriction Enzymes Cut DNA sequences at specific repeating patterns. EcoRI cuts at GAATTC which will appear at different points.

Genetic Engineering. Restriction Enzymes Cut DNA sequences at specific repeating patterns. EcoRI cuts at GAATTC which will appear at different points.

4.8 Les marqueurs génétiques moléculaires (ou …€¦ · = GAATTC = site de coupure par l’enzyme de restriction EcoRI 49 SNP :? GAATTC CTTAAG Test expérimental pour génotyper

4.8 Les marqueurs génétiques moléculaires (ou …€¦ · = GAATTC = site de coupure par l’enzyme de restriction EcoRI 49 SNP :? GAATTC CTTAAG Test expérimental pour génotyper

25 February, 2005 Chapter 10 Gene Mutation: Origins and Repair Processes GAATTC GTATTC A a.

25 February, 2005 Chapter 10 Gene Mutation: Origins and Repair Processes GAATTC GTATTC A a.

Growth Hormone (GH)mcb.berkeley.edu/courses/mcb41/lecture_17.pdfReview of recombinant DNA technology Growth Hormone Reading for this and next lecture: Chapter 19 GAATTC CTTAAG G CTTAA

Growth Hormone (GH)mcb.berkeley.edu/courses/mcb41/lecture_17.pdfReview of recombinant DNA technology Growth Hormone Reading for this and next lecture: Chapter 19 GAATTC CTTAAG G CTTAA

Analysis of the yeast SPT3 gene and Identification of its ...-winston-1986.pdf · Xhol fragment in pBR322 (29)( for Ty, B161, carrying a Ty Bglll restriction fragment in pBR322 (R.

Analysis of the yeast SPT3 gene and Identification of its ...-winston-1986.pdf · Xhol fragment in pBR322 (29)( for Ty, B161, carrying a Ty Bglll restriction fragment in pBR322 (R.

GAATTC AATGGCATAC-3’ TTACCGTATC-5’ - Babec · functions of the genes and occur ... contribute a hair for DNA extraction. ... Change tip every time the pipette is used even if

GAATTC AATGGCATAC-3’ TTACCGTATC-5’ - Babec · functions of the genes and occur ... contribute a hair for DNA extraction. ... Change tip every time the pipette is used even if

Lecture 6: Molecular Techniques · 2007. 7. 31. · Lecture 6: Molecular Techniques 1. Restriction Mapping 2. Molecular cloning ... into gel. Run gel. Ban-HI Gel results BamHl sites

Lecture 6: Molecular Techniques · 2007. 7. 31. · Lecture 6: Molecular Techniques 1. Restriction Mapping 2. Molecular cloning ... into gel. Run gel. Ban-HI Gel results BamHl sites

A Novel Expression Vector for the Cyanobacterium ......182 Expression Vector for Synechococcus PCC 6301 [Vol. 1, a. pBA1 (7.8 kb) b. pBR322M (4.3 kb) Hindlll Bglll Hpal Pvull Xhol

A Novel Expression Vector for the Cyanobacterium ......182 Expression Vector for Synechococcus PCC 6301 [Vol. 1, a. pBA1 (7.8 kb) b. pBR322M (4.3 kb) Hindlll Bglll Hpal Pvull Xhol

Phagedisplayofricin Bchainandits single bindingdomains ...SBD1 Pst1i-j SBD2 46 N'1 T143-/DFXhoI N2655 F262 1-XhoI T143 100 bp F Xhol 26 262 255 F N FIG. 1. Structure ofgene 3 (A) and

Phagedisplayofricin Bchainandits single bindingdomains ...SBD1 Pst1i-j SBD2 46 N'1 T143-/DFXhoI N2655 F262 1-XhoI T143 100 bp F Xhol 26 262 255 F N FIG. 1. Structure ofgene 3 (A) and