Kleuren met:Prisma® & Coverslipper HQplus

Door: Jorrit van het Bolscher

Aplication specialist Sakura

Agenda

Update Prisma & Coverslipper HQplus

Kleuringen (H&E) Bij de gebruiker

Wat als: De Kleuring niet mooi of constant is……..

Prisma®

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Throughput & speed Proven reliabilityFlexibility

Routine

&

Special660

slides/hour

• Continuously loading and 11 protocols simultaneously running!

Introducing: Prisma® & Coverslipper HQplus

Introducing: Prisma® & Coverslipper HQplus

HQplus

• New & improved H&E formula in combination with

optimized & validated protocols

• Consistent high-quality staining up to 2.000 slides

• Integrated barcode reader for efficient slide tracking

• Fastest multi-stainer and coverslipper

Prisma® HQ Staining

New and improved H&E staining formula

• Proven consistent high-quality staining up to 2.000 slides

• Packaging for ease of use and increased safety

• Efficiency and time saving

• Integration by system matching volumes

• Flexibility to optimize staining results

Results H&E Staining, optimized formula

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Placenta Colon Prostate

Slide 1

Slide 1.000

Slide 1.500

Slide 2.000

HQ staining: an example in more detail

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Examples: Placenta

3 tones of pink (eosin) staining Erytrocytes, red blood cells Connective tissue Connective tissue

Haematoxyline Crisp blue/purple nuclear detail

H&E Staining Kit 270 mL

• Item 8640 for Medium Solution reservoirs

H&E Staining Kit 750 mL

• Item 8641 for Large Solution reservoirs

Ready to Use (RTU) H&E Staining Kits

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Prisma® Coverslipper Plus

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Film Glas g2

Plus: Barcode reader integration

Barcode reader to support track and trace capabilities

• Barcode reader sends a file in data form via USB

• Results can be sent to an LI(M)S or stand alone computer

• Results are connected to Track and Trace System to verify finished Coverslipping

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Plus: Barcode reader integration

Benefits

• Feedback when slides are ready for reporting• Reduction of recording errors • Free up resources: manual scanning of the slides• Easier tracing of root causes

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Summary: Prisma® & Coverslipper HQplus

Optimized Staining results• Ready-to-use H&E Staining kits• Documentation and specialized support• Easy of use and increased safety

Consistent Staining result• Validated Sakura protocols• Clear cut off point to replace reagents

Integrated Slide Barcode Reading Track when the slides are ready Aid with tracing root cause Free resources for more value adding work

Maximized productivity • Fastest system in the market with highest throughput

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Voorbeelden Gebruik Prisma®

1 Klant voorbeeld HE gebruik gekoppeld aan FILM Afdekmachine

2 Klant voorbeeld HE en PAP Kleuring gekoppeld aan FILM Afdekmachine

3 Klant voorbeeld HE en Speciaal Kleuringen gekoppeld aan FILM Afdekmachine

Voorbeeld Gebruik Prisma®:1: HE gebruik gekoppeld aan FILM Afdekmachine

Voorbeeld Gebruik Prisma®:1: HE gebruik gekoppeld aan FILM Afdekmachine

• Program Name:HE+OVEN•

• Step Station Solution Time Delay Mix• 1 S* Start Station --:--:--• 2 D* Drying Station 00:15:00 ** OFF• 3 21 Xylene 00:02:00 ** OFF• 4 20 Xylene 00:02:00 ** OFF• 5 19 Xylene 00:02:00 ** OFF• 6 18 Alcohol 100% 00:02:00 ** OFF• 7 17 Alcohol 100% 00:02:00 ** OFF• 8 9 Alcohol 100% 00:02:00 ** OFF• 9 10 Alcohol 90% 00:02:00 ** OFF• 10 1 Alcohol 70% 00:02:00 ** OFF• 11 W* Wash Station 00:02:00 ** OFF• 12 2 Hematoxylin(Mayers) 00:03:00 20% EW• 13 3 Hematoxylin(Mayers) 00:03:00 20% ON• 14 W* Wash Station 00:05:00 20% EW• 15 11 Ammonia Water 00:00:27 == ON• 16 W* Wash Station 00:01:00 50% OFF• 17 12 Erytrosine 00:01:30 20% ON• 18 W* Wash Station 00:01:00 20% EW• 19 13 Alcohol 80% 00:01:00 20% OFF• 20 14 Alcohol 100% 00:01:00 ** OFF• 21 15 Alcohol 100% 00:01:00 ** OFF• 22 23 Alcohol 100% 00:01:00 ** OFF• 23 22 Alcohol 100% 00:01:00 ** OFF• 24 29 Xylene 00:02:00 ** OFF• 25 28 Xylene 00:02:00 ** OFF• 26 E* End Station --:--:--• Total 26 Steps

Voorbeeld Gebruik Prisma®:2: HE en PAP kleuring met FILM Afdekmachine

• Program Name: HE•

• Step Station Solution Time Delay Mix• 1 S* Start Station --:--:--• 2 D* Drying Station 00:10:00 ** OFF• 3 17 Xyleen 00:01:30 ** ON• 4 9 Xyleen 00:01:30 ** OFF• 5 1 Iso 100 00:01:30 ** OFF• 6 2 Methanol 00:01:30 ** OFF• 7 W* Wash Station 00:01:30 ** OFF• 8 15 Hematoxyline 00:03:00 20% ON• 9 W* Wash Station 00:01:30 20% OFF• 10 3 HCL 00:00:30 20% ON• 11 W* Wash Station 00:00:30 20% OFF• 12 14 Li Carbonaat 00:00:30 20% ON• 13 W* Wash Station 00:00:30 20% OFF• 14 13 Erythrosine 00:00:30 20% ON• 15 W* Wash Station 00:00:30 20% OFF• 16 11 Iso 100 00:01:30 ** ON• 17 10 Iso 100 00:01:30 ** OFF• 18 18 Xyleen 00:01:30 ** ON• 19 19 Xyleen 00:01:30 ** OFF• 20 E* End Station --:--:--

• Program Name: PAP•

• Step Station Solution Time Delay Mix• 1 S* Start Station --:--:--• 2 W* Wash Station 00:03:00 ** OFF• 3 15 Hematoxyline 00:02:00 ** ON• 4 W* Wash Station 00:02:00 ** ON• 5 28 Iso 70 00:01:30 ** OFF• 6 23 Orange G 00:01:00 20% ON• 7 22 Iso 70 00:02:00 ** OFF• 8 21 Iso 70 00:02:00 ** OFF• 9 27 Light Green 00:02:00 ** ON• 10 20 Iso 70 00:02:00 ** ON• 11 12 Iso 100 00:02:00 ** OFF• 12 18 Xyleen 00:01:30 ** ON• 13 19 Xyleen 00:01:30 ** ON• 14 E* End Station --:--:--

• Overige programma’s

Voorbeeld Gebruik Prisma®:3: HE en Speciaal kleuringen met FILM Afdekmachine

Voorbeeld Gebruik Prisma®:3: HE en Speciaal kleuringen met FILM Afdekmachine

Program Name:HE

Step Station Solution Time Delay Mix1 S* Start Station --:--:--2 D* Drying Station 00:10:00 ** OFF3 44 Xylene 00:02:00 ** OFF4 43 Xylene 00:02:00 ** OFF5 41 Alcohol 100% 00:02:00 ** OFF6 42 Alcohol 100% 00:02:00 ** OFF7 40 Alcohol 70% 00:02:00 ** OFF8 W* Wash Station 00:02:00 ** OFF9 39 A DEST 00:02:00 ** OFF10 37 Haematox 00:02:00 ** OFF11 W* Wash Station 00:03:00 ** OFF12 6 Alcohol 70% 00:02:00 ** OFF13 1 Alcohol 100% 00:02:00 ** OFF14 2 Eosin Y 00:01:30 ** OFF15 15 Alcohol 100% 00:02:00 ** OFF16 16 Alcohol 100% 00:02:00 ** OFF17 31 Xylene 00:02:00 ** OFF18 32 Xylene 00:03:00 ** OFF19 E* End Station --:--:--

Total 19 Steps

Program Name:PAS Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 D* Drying Station 00:10:00 ** OFF 3 28 Xylene 00:01:00 ** OFF 4 27 Xylene 00:01:00 ** OFF 5 25 Alcohol 100% 00:01:00 ** OFF 6 26 Alcohol 100% 00:01:00 ** OFF 7 24 Alcohol 70% 00:01:00 ** OFF 8 23 A DEST 00:01:00 ** OFF 9 20 Perj 1% 00:10:00 ** OFF 10 W* Wash Station 00:01:00 ** OFF 11 21 A DEST 00:01:00 ** OFF 12 19 SCHIFF`S 00:20:00 ** OFF 13 W* Wash Station 00:05:00 ** OFF 14 22 Hematoxylin 00:01:00 ** OFF 15 W* Wash Station 00:03:00 ** OFF 16 17 Alcohol 100% 00:01:00 ** OFF 17 18 Alcohol 100% 00:02:00 ** OFF 18 33 Xylene 00:01:00 ** OFF 19 34 Xylene 00:02:00 ** OFF 20 E* End Station --:--:-- Total 20 Steps

Voorbeeld Gebruik Prisma®:3: HE en Speciaal kleuringen met FILM Afdekmachine

Program Name:EVG Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 D* Drying Station 00:10:00 ** OFF 3 28 Xylene 00:01:00 ** OFF 4 27 Xylene 00:01:00 ** OFF 5 25 Alcohol 100% 00:01:00 ** OFF 6 26 Alcohol 100% 00:01:00 ** OFF 7 24 Alcohol 70% 00:01:00 ** OFF 8 23 A DEST 00:01:00 ** OFF 9 3 Elastica 00:30:00 ** OFF 10 W* Wash Station 00:02:00 ** OFF 11 4 Van Gieson's 00:10:00 ** OFF 12 17 Alcohol 100% 00:01:00 ** OFF 13 18 Alcohol 100% 00:01:00 ** OFF 14 33 Xylene 00:01:00 ** OFF 15 34 Xylene 00:02:00 ** OFF 16 E* End Station --:--:-- Total 16 Steps

Program Name:ALCIAN BLUE Step Station Solution Time Delay Mix 1 S* Start Station --:--:-- 2 D* Drying Station 00:10:00 ** OFF 3 28 Xylene 00:02:00 ** OFF 4 27 Xylene 00:02:00 ** OFF 5 25 Alcohol 100% 00:02:00 ** OFF 6 26 Alcohol 100% 00:02:00 ** OFF 7 24 Alcohol 70% 00:02:00 ** OFF 8 23 A DEST 00:02:00 ** OFF 9 36 Alcian Blue 00:30:00 ** OFF 10 W* Wash Station 00:01:00 ** OFF 11 35 Kernecht rood 00:05:00 ** OFF 12 W* Wash Station 00:01:00 ** OFF 13 17 Alcohol 100% 00:02:00 ** OFF 14 18 Alcohol 100% 00:02:00 ** OFF 15 33 Xylene 00:02:00 ** OFF 16 34 Xylene 00:03:00 ** OFF 17 E* End Station --:--:-- Total 17 Steps

Wat als:

Application Bulletin H&E Staining kits V-1.pdf

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Bedankt voor jullie aandacht!