Kentuck- E . Derby
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Transcript of Kentuck- E . Derby
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Nathan Reyna, BiologyJeff Matocha, Computer ScienceOuachita Baptist University
Kentuck-E. Derby
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Project GoalsProject Goals
Active, hands-on approach to teaching basic Active, hands-on approach to teaching basic biology and computer science conceptsbiology and computer science concepts
Encourage abstract thinkingEncourage abstract thinking
Keeps students/faculty engaged in a Keeps students/faculty engaged in a semester long project.semester long project.
Have funHave fun
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Project OverviewProject Overview
GoalGoal– To engineer a biological “wire” capable of To engineer a biological “wire” capable of
propagating a chemical signal down its propagating a chemical signal down its lengthlength
Initial Signal
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Harvard iGEM 2005
Orr AshenbergPatrick BradleyConnie ChengChris DoucetteHing EngJennifer Gao
Kang-Xing JinYin LiThomas NoriegaDaniel PopperAlexander RushYves Wang
Doh!Doh!
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RequirementsRequirements
Inducible PromoterInducible Promoter
Product/Signal that activates promoters in Product/Signal that activates promoters in other cellsother cells– Secrete product outside of cellSecrete product outside of cell
Cell to cell communication/Quorum sensingCell to cell communication/Quorum sensing
Detection MethodDetection Method
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MethodMethod
Signal: acyl-homoserine lactones (AHL) Signal: acyl-homoserine lactones (AHL) used in bacterial quorum sensingused in bacterial quorum sensing– Lux system: 3OC6HSLLux system: 3OC6HSL– Las system: 3OC12HSLLas system: 3OC12HSL– Sender device Sender device
Other promoters associated with cell to Other promoters associated with cell to cell communicationcell communication– Most are “in planning” stageMost are “in planning” stage
– LacI generatorLacI generator– LacI promoterLacI promoter
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Harvard’s ConclusionsHarvard’s Conclusions
ConclusionsConclusions– Degradation tags, RBS/promoter strength Degradation tags, RBS/promoter strength
may need fine-tuningmay need fine-tuning– Because of positive feedback, noise is Because of positive feedback, noise is
amplifiedamplified– Further experiments necessaryFurther experiments necessary
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GFP production across plateGFP production across plate
Change in pH gradientChange in pH gradient
Vernier conductivity probeVernier conductivity probe– Senses changes in pHSenses changes in pH– Connects to a computerConnects to a computer– Currently use probes in Freshman Bio and Gen Currently use probes in Freshman Bio and Gen
ChemChem
Tracking the HorsesTracking the Horses
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Data acquisitionData acquisition– interfacing with probesinterfacing with probes
Data reportingData reporting
CS student projectsCS student projects
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Computer ScienceComputer Science
Time Time
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Not just a one heat raceNot just a one heat race
Reduce pathway bottlenecksReduce pathway bottlenecks
Diffusion rates/distance between cellsDiffusion rates/distance between cells
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QuestionsQuestions
What promoter/signal pairs do we have?What promoter/signal pairs do we have?
Are they secreted outside the cell?Are they secreted outside the cell?