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Study of bacteria isolated from urinary

tract infections and determination oftheir susceptibility to antibiotics

Pembimbing :

dr. Dasril Nizam, Sp.PD, KGEH

Disusun oleh :Maya Andriana

07120080048

FK UNIVERSITAS PELITA HARAPAN

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INTRODUCTION

Urinary tract infection (UTI) is the second mostcommon infectious in community medicalpractice.

150 million people are diagnosed UTI each year. UTI are classified :

Uncomplicated healthy female patients with structurally and functionally

normal urinary tracts.

Complicated abnormalities of the urinary tract that impede urine flow,

the existence of a foreign body (e.g., indwelling catheter,stone), or infection with multi- drug resistant pathogens.

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UTI

the lower urinary tract or both theupper and the lower tracts.

Cystitis : dysuria, frequency, and occasionally

suprapubic tenderness.

Acute pyelonephritis : flank pain or tenderness, or

both, and fever, often associated with dysuria,

urgency, and frequency.

> 95% of urinary tract infections are caused bya single bacterial species (E. coli).

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Corynebacterium urealyticum important

nosocomial pathogen.

Anaerobic organisms are rarely pathogens in

the urinary tract.

Coagulase Negative Staphylococci are a

common cause of UTI in some reports.

Staphylococci saprophyticus in young

women of a sexually active age.

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MATERIALS AND METHODS

- Sampling-

In total, 7056 patients with clinical symptoms

of UTI referred to Imam Khomeini hospital

Ahvaz, Iran, were investigated. 4209 (50.7%) females and 2847 (40.3%) males.

age range of 25-60 years (mean, 43.7 years).

Clean-Catch midstream urine of the patients wascollected in a sterile tube (4-5 ml).

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MATERIALS AND METHODS

- Antimicrobial susceptibility testing -

Study antimicrobial susceptibility testing wasdone on Mueller- Hinton agar.

ampicilin (10g), kanamicin (30g),cephalothin (30g), ciprofleoxacin (5g),tetracycline (30g), nalidixic acid (30g),nitofurantoin (300g), amikacin (30g),

tobramicin (10g), ceftriaxone (30g),cefotaxime (10g)and gentamicin (10g)andtrimethoprim-sulfamethoxazole (25g)

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RESULTS

In this study, 553(8.7%) patients out of 7056

were showed to be urine culture positive

(their colony count was equal or more than104).

There were 376(68%) females and 177(32%)

males in patients with urine positive culture.

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RESULTS

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RESULTS

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DISCUSSION

Approximately 1 in 3 women will require

antimicrobial treatment for a UTI before age

24, and 40% - 50% of women will have a UTIduring their lifetime.

The elevated incidence of UTI among females

(between male) is related to genitourinarysystems in anatomy and microflora.

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DISCUSSION

The prevalence of Gram-positive cocci was not high inour study ; this is similar to other studies in differentcountries.

E. coli was the most predominant bacteria. In present study a high level of bacterial resistance

ampicillin and cephalothin. (similar to previous studiesin the United States).

The most effective antimicrobial agents in our studyand other reported researchesamikacin andciprofleoxacin for Gram- negative bacilli (81%-100%).

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TERIMA KASIH