JOURNAL OF BACTERIOLOGYVOLUME 167 SEPTEMBER 1986 NUMBER 3

Simon Silver, Editor in Chief (1987)University of Illinois, Chicago

James D. Friesen, Editor (1987)University of Toronto,Toronto, Canada

Stanley C. Holt, Editor (1987)The University of Texas HealthScience Center, San Antonio

Samuel Kaplan, Editor (1988)University of Illinois, Urbana

June J. Lascelles, Editor (1989)University of California, Los AngelesRichard M. Losick, Editor (1988)Harvard University, Cambridge, Mass.

L. Nicholas Ornston, Editor (1987)Yale University, New Haven, Conn.

Robert H. Rownd, Editor (1990)Northwestern Medical School,Chicago, Ill.

Graham C. Walker, Editor (1990)Massachusetts Institute of

Technology, Cambridge, Mass.

Robert A. Weisberg, Editor (1990)National Institute of ChildHealth and HumanDevelopment, Bethesda, Md.

EDITORIAL BOARDDavid Apirion (1988)Stuart J. Austin (1987)Frederick M. Ausubel (1986)Gad Avigad (1986)Barbara Bachmann (1987)Manfred E. Bayer (1988)Margret H. Bayer (1986)Claire M. Berg (1986)Helmut Bertrand (1988)Terry J. Beveridge (1988)Edwin Boatman (1986)Donald A. Bryant (1988)Joseph M. Calvo (1987)A. M. Chakrabarty (1986)Keith F. Chater (1988)Terence G. Cooper (1987)John E. Cronan, Jr. (1986)Jorge H. Crosa (1988)Walter B. Dempsey (1986)Patrick Dennis (1988)W. Ford Doolittle (1988)David A. Dubnau (1986)Martin Dworkin (1988)Bert Ely (1988)

Wolfgang Epstein (1987)David Figurski (1987)Timothy J. Foster (1986)Robert T. Fraley (1988)Masamitsu Futai (1988)Robert Gennis (1988)Jane Gibson (1988)Robert D. Goldman (1988)E. Peter Greenberg (1988)Scott R. Hagedorn (1988)Barry G. Hall (1988)R. E. W. Hancock (1987)Richard S. Hanson (1988)Robert Haselkorn (1987)Gerald L. Hazelbauer (1987)Dennis Henner (1988)Charles L. Hershberger (1987)Penny J. Hitchcock (1987)James Hopper (1988)Martha M. Howe (1987)Karin Ihler (1987)A. W. B. Johnston (1986)Eva R. Kashket (1987)David E. Kennell (1988)

Wil N. Konings (1987)Jordan Konisky (1987)Dennis J. Kopecko (1987)Viji Krishnapillai (1988)Terry Krulwich (1987)Lasse Lindahl (1987)Jack London (1987)Paul S. Lovett (1987)Ben J. J. Lugtenberg (1986)Robert Macnab (1988)Francis L. Macrina (1986)Sarah A. Mclntire (1986)Larry McKay (1987)S. Mizushima (1988)Edward A. Morgan (1987)Gisela Mosig (1988)R. G. E. Murray (1986)Robert A. Niederman (1988)Allen T. Phillips (1988)Patrick J. Piggot (1987)Barry Polisky (1987)Linda Randall (1987)Marilyn Roberts (1986)

Palmer Rogers (1987)Barry P. Rosen (1986)Rudiger Schmitt (1986)June R. Scott (1987)Jane K. Setlow (1987)Peter Setlow (1987)James A. Shapiro (1988)Lucille Shapiro (1986)Louis A. Sherman (1988)Howard A. Shuman (1988)Issar Smith (1987)John L. Spudich (1988)Catherine Squires (1987)Robert Switzer (1987)Alexander Tomasz (1986)Godfried D. Vogels (1987)Barry Wanner (1987)Bernard Weisblum (1986)Malcolm Winkler (1988)David Womble (1986)Henry C. Wu (1987)Duane C. Yoch (1986)Howard Zalkin (1988)

Helen R. Whiteley, Chairman, Publications BoardLinda M. Illig, Managing Editor, Journals

Kirk Jensen, Director of PublicationsDennis G. Burke, Production Editor

The Journal of Bacteriology (ISSN 0021-9193), a publication of the American Society for Microbiology, 1913 I St., NWWashington, DC 20006, is devoted to the advancement and dissemination offundamental knowledge concerning bacteria andother microorganisms. Instructions to authors are published in the January issue each year; reprints are available from theeditors and the Publications Department. The Journal is published monthly, and the twelve numbers are divided into fourvolumes per year. The nonmember subscription price is $280 per year; single copies are $21. The member subscription price is$41 (foreign, $54 [surface rate]) per year; single copies are $7. Correspondence relating to subscriptions, reprints, defectivecopies, availability of back issues, lost or late proofs, disposition of submitted manuscripts, and general editorial mattersshould be directed to the ASM Publications Department, 1913 I St., NW, Washington, DC 20006 (phone: 202 833-9680).Claims for missing issues from residents of the United States, Canada, and Mexico must be submitted within 3 months afterpublication of the issues; residents of all other countries must submit claims within 6 months of publication of the issues.Claims for issues missing because offailure to report an address change or for issues "missing from files" will not be allowed.Second-class postage paid at Washington, DC 20006, and at additional mailing offices.POSTMASTER: Send address changes to Journal of Bacteriology, ASM, 1913 I St., NW, Washington, DC 20006.Made in the United States of America.Copyright © 1986, American Society for Microbiology. a* : 1MHI5Y0A L U:trw%kJ (IPIfoAll Rights Reserved.The code at the top of the first page of an article in this journal indicates the copyright owner's consent that copies of the arti-cle may be made for personal use or for personal use of specific clients. This consent is given on the condition, however, thatthe copier pay the stated per-copy fee through the Copyright Clearance Center Inc., 21 Congress St., Salem, MA 01970, forcopying beyond that permitted by Sections 107 and 108 of the U.S. Copyright Law. This consent does not extend to otherkinds of copying, such as copying for general distribution, for advertising or promotional purposes, for creating new collectiveworks, or for resale.

Author IndexAlexander, Patrick, 870Ambulos, Nicholas P., Jr.,

842Arias, Alicia, 1092

Bantle, John A., 940Barbe, Jordi, 1055Baumeister, Wolfgang, 1048Bayer, Edward A., 828Begg, Kenneth J., 1004Bisher, Margaret E., 968Bradbeer, Clive, 920

Cairo, Jordi, 1055Cervefiansky, Carlos, 1092Chang, Chung-Fu, 935Chang, Shing, 952Clevenson, Dina S., 920Correia, Frederick F., 1009Cowell, James L., 968Crawford, Ronald L., 818

Daneo-Moore, Lolita, 960Daugherty, Robin M., 805Davila, Guillermo, 1043de Maagd, Ruud A., 1083DeVeaux, Linda C., 920, 928Dicker, David T., 960Dickson, M. R., 1025Doi, Masaki, 975Donachie, William D., 809,

1004,Dougan, Gordon, 799Downing, K. H., 1025Duvall, Elizabeth J., 842

Fahnestock, Stephen R., 870Falk, Leon, 766Ferenci, Thomas, 1081Filpula, David, 870Finkelthal, Ophir, 766Folkerts, Otto, 774Foster, John W., 1086Friedrich, M. J., 928Fusselman, Robert, 1035

Garrett, Janetta, 1035Garro, Anthony, 766Glaeser, R. M., 1025Grimaila, Ray, 1035Gross, Zvi, 986Guerrero, Ricardo, 1055Guffanti, Arthur A., 766

Heller, Knut J., 1071Henner, Dennis J., 792Hernandez-Chico,Concepci6n, 1058

Hicks, David B., 766Higgins, Michael L., 960Hoover, Timothy R., 999Howe, Martha M., 905Huschka, Hans-Georg, 1020

Inamine, Julia M., 855Inatomi, Ken-ichi, 837Inoue, Matsuhisa, 975Inouye, Masayori, 1009Inouye, Sumiko, 1009Ishino, Fumitoshi, 975Iyer, Shankar, 893

Jalal, Mahbubul A. F., 1020Johnson, William, 893

Kadner, Robert J., 920, 928Kanemasa, Yasuhiro, 1016Kenan, Daniel J., 809Kitano, Kazuaki, 759Koch, Arthur L., 960Kolter, Roberto, 1058Kondorosi, Adam, 881Konno, Masatoshi, 975Krauel, Verena, 1071Krulwich, Terry A., 766Kuroda, Mitzi I., 792

Lamed, Raphael, 828Lampson, Bert C., 888LeBlanc, Donald J., 855Lee, Kin-Sang, 1081

Lee, Linda N., 855Lewis, Thomas A., 981Lindahl, Lasse, 1095Lorenz, R. Todd, 981Lovett, Paul S., 842Ludden, Paul W., 999Lugtenberg, Ben, 1083Lynn, Melanie E., 940

Matsuhashi, Michio, 975McCabe, J. B., 1066McLaughlin, Jane R., 952Montgomery, Rex, 893Moon, Harley W., 799Moreno, Felipe, 1058Moseley, Steve L., 799Mutoh, Norihiro, 992

Nagle, James, 870Neal, Gregory, 1035Noda, Yasuko, 1016Noti, John D., 774, 784

Oosawa, Kenji, 992Otten, Sharee, 893Ownby, James D., 940

Parisi, Joseph T., 888Parks, Leo W., 981Peters, Jurgen, 1048Porter, R. D., 1074Putnoky, Peter, 881

Raab, Ronald, 1035Roberts, Gary P., 999Robinson, Arthur C., 809Rodriguez, Russell J., 981Romano, Nino, 1089Romero, David, 1043Ross, Wilma, 905Rottem, Shlomo, 986, 1089Ruby, E. G., 1066

Sako, Tomoyuki, 850San Millin, Jose Luis, 1058

Schneider, Robert A., 799Scopes, Robert K., 863Shah, Vinod K., 999Shimotsu, Hidenori, 792Shirvan, Mitchell H., 1089Shore, Scott H., 905Shuman, Henry, 935Sidhu, Anita, 766Simon, Melvin I., 992Somlyo, Andrew P., 935Song, Min Dong, 975Spector, Michael P., 1086Spratt, Brian G., 1004Steven, Alasdair C., 968Stevens, S. E., Jr., 1074Sweeney, Janis, 809Szalay, Aladar A., 774, 784

Thomas, Daniel, 968Ting, Yi E., 952Tirgari, Simin, 1086Tomasek, Paul H., 818Tomasz, Alexander, 759, 1077Trus, Benes L., 968Tuomanen, Elaine, 759, 1077Turken, Arthur N., 774

Ubukata, Kimiko, 975Ugalde, Rodolfo A., 947

Van Arsdell, Janelle N., 952van der Helm, Dick, 1020Villaverde, Antonio, 1055

Wachi, Masaaki, 975Winkelmann, Gunther, 1020Winkler, Herbert H., 805Wong, Hing C., 952Wu, W. H., 1025

Yamashita, Naoko, 975Yanofsky, Charles, 792Young, Ry, 1035Yun, Allen C., 784

Zachariou, Michael, 863Zengel, Janice M., 1095Zhang, Jia Ming, 968Zorreguieta, Angeles, 947

AUTHOR INDEX

VOLUME 167

Actis, Luis A., 57Adar, Lyrit, 299Akiyama, Yoshinori, 201Alexander, Patrick, 870Allen, Caitilyn, 279Altamura, Sergio, 265Altuvia, Shoshy, 415Amaral, Leonard, 492Ambulos, Nicholas P., Jr.,

842Amils, Ricardo, 265Andersson, Kristoffer K., 735Ang, Debbie, 25Ankenbauer, Robert, 7Aranda, Armando, 544Arias, Alicia, 1092Armstrong, G. D., 660

Banner, Carl D. B., 623Bantle, John A., 940Barbe, Jordi, 1055Baron, L. S., 191Barron, Anne, 433Barth, Peter T., 12Baumann, Marlene, 191Baumeister, Wolfgang, 1048Bayer, Edward A., 828Beatty, J. Thomas, 604Beaty, P. Shawn, 179Beck, D. A., 285Beck, Pamela J., 251Beckwith, Jon, 160Begg, Kenneth J., 1004Belas, Robert, 210Beppu, Teruhiko, 110Bergbauer, H., 368Bergmans, Hans, 407Berman, Phyllis M., 279Biek, Donald P., 594Bisher, Margaret E., 968Bishop, Paul E., 480Biswas, Gour D., 685Blakemore, Richard P., 73,729

Blankenship, R. E., 655Bock, August, 265Bohall, Nicholas A., Jr., 716Boman, Hans G., 18Boogerd, Fred C., 30Boos, Winfried, 462Boylan, Sharon, 82Bradbeer, Clive, 920Brambl, Robert, 124Brasch, Michael A., 703Bremer, Erhard, 433Brey, Robert N., 623Broekhuizen, Cornelis P., 393Bullerjahn, George S., 396Burdett, I. D. J., 219

Cairo, Jordi, 1055Cammarano, Piero, 265Campbell, John W., 49Cannon, Janne G., 186Carbonetti, Nicholas H., 467Casadaban, Malcolm J., 639,

711Cervefiansky, Carlos, 1092Chakrabarty, A. M., 611Chakraborty, T., 368Chandrasekhar, G. N., 25

Chang, Chung-Fu, 935Chang, Ming Chung, 551Chang, Shing, 952Chang, Ying-Ying, 312Chen, Lingling, 389Chilton, Mary-Dell, 387Chye, Mee-Len, 749Clark, Alvin J., 327Clark, David P., 362Clevenson, Dina S., 920Cohen, Amikam, 327Cohen, Stanley N., 594Coleno, Alain, 400Collins, Mary Lynne Perille,

89Collmer, Alan, 117Condreay, J. Patrick, 251Cooper, Ronald A., 467Correia, Frederick F., 1009Cossart, Pascale, 639Courvalin, Patrice, 631Cowell, James L., 968Cox, Charles D., 7Crawford, Ronald L., 818Cronan, John E., Jr., 312Crook, Suzanne M., 89Crosa, Jorge H., 57, 647Curtis, Michael A., 522Czechowski, Melvin H., 722

Daneo-Moore, Lolita, 562, 960Daugherty, Robin M., 805Davidson, Barrie E., 556Davila, Guillermo, 1043Davis, Philip J., 299Dean, Donald H., 719de Boer, Mark, 393Del-Arenal, Patricia, 544Del Bue, Marina, 740de Lorenzo, Victor, 350de Maagd, Ruud A., 1083DeMoll, Edward, 681Dempsey, Walter B., 336Denoya, Claudio, 138De Ree, Han, 407Deretic, Vojo, 611Dessaux, Yves, 732DeVeaux, Linda C., 920, 928De Vos, Guido F., 66de Vrind, Johannes P. M., 30de Vrind-de Jong, ElisabethW., 30

Dicker, David T., 960Dickson, M. R., 1025Diolez, Annick, 400Dobrogosz, Walter J., 616Doi, Masaki, 975Donachie, William D., 809,

1004,Dougan, Gordon, 799Downing, K. H., 1025Drews, Gerhart, 96Dubnau, David, 138Duvall, Elizabeth J., 842

Ecker, David J., 666, 674Elhai, Jeffrey, 411Ellar, David J., 257Elleman, Thomas C., 243Ellenberger, Suzanne R., 57Ely, Susan, 44

Escamilla, Jose E., 544Esch, Keith, 82

Fahnestock, Stephen R., 870Fahrney, David E., 49Falk, Leon, 766Fall, Ray, 496Farrand, Stephen K., 732Fee, Brian E., 336Ferenci, Thomas, 1081Ferrero, Maria, 562Ferretti, Joseph J., 631Filpula, David, 870Finan, Turlough M., 66Finkelthal, Ophir, 766Finlay, B. Brett, 660, 754Firshein, William, 319Fischer, Meir, 423Fish, William, 57Fliss, Edward R., 168Foerster, Harold F., 168Folkerts, Otto, 774Follettie, Max T., 695Foltermann, K. F., 285Ford, Steven, 467Foster, J. M., 655Foster, John W., 1086Fowler, Robert G., 130Frank, Rainer, 96Fridovich, Irwin, 346Friedman, David I., 191Friedrich, M. J., 928Frost, Laura S., 660, 754Fuller, R. C., 655Fusselman, Robert, 1035

Garrett, Janetta, 1035Garro, Anthony, 766Geller, Tanya, 423Georgopoulos, Costa, 25Gicquel-Sanzey, Brigitte, 639Giddings, Thomas H., 496Gill, John F., 611Gilmore, Keeta S., 631Glaeser, R. M., 1025Glickman, Barry W., 130Goebel, W., 368Goodman, Thomas C., 387Goosen, Nora, 503Grandi, Guido, 740Graves, James, 685Greenberg, Elaine, 82Grimaila, Ray, 1035Groisman, Eduardo A., 639Gross, Zvi, 299, 986Guerrero, Ricardo, 1055Guest, John R., 749Guffanti, Arthur A., 766Guiney, Donald G., 517Gunsalus, Glen L., 272Gunsalus, Robert P., 272Guy, C. L., 124

Hagblom, Per, 231Hall, Ruth M., 420Hamood, Abdul N., 375Hancock, Robert E. W., 473Hanne, Larry F., 7, 473Harayama, S., 455Harper, Stuart H., 49Hatch, T. P., 570

Hauser, Frank M., 57Hazelbauer, Gerald L., 101Hederstedt, Lars, 735Heierson, Agneta, 18Heller, Knut J., 1071Hengge-Aronis, Regine, 462Henner, Dennis J., 792Hernandez-Chico,Concepci6n, 1058

Hicks, David B., 766Higgins, Michael L., 562, 960Hildebrand, Eilo, 305Hodges, R. S., 660Hoekstra, Wiel, 407Hook, Magnus, 77Hoover, Timothy R., 999Horikoshi, Koki, 404Howe, Martha M., 905Hoyne, Peter A., 243Huhle, B., 368Hummel, Heidi, 265Hunt, John F., 160Huschka, Hans-Georg, 1020Hutchinson, C. Richard, 575,

581Huynh, Boi Hanh, 674

landolo, John J., 726lglewski, Barbara H., 473Ihara, Norihiko, 148lismaa, T. P., 379Inamine, Julia M., 855Inatomi, Ken-ichi, 837lnoue, Matsuhisa, 975Inouye, Masayori, 1009Inouye, Sumiko, 1009Ishino, Fumitoshi, 975Ito, Eiji, 508Ito, Koreaki, 201Iwasaki, Hiroyoshi, 508lyer, Shankar, 893

Jackson, Matthew P., 726Jacobson, Marty R., 480Jahnke, Linda L., 238Jalal, Mahbubul A. F., 1020Joerger, Melissa C., 439Johnson, Jeanette A., 604Johnson, William, 893Johnstone, Keith, 257Jonsson, Ann-Beth, 231

Kadner, Robert J., 920, 928Kanemasa, Yasuhiro, 1016Kasian, Paul A., 556Kawai, Shinya, 551Kawakami, Bunsei, 404Kellerman, Katherine, 57Kenan, Daniel J., 809Kirkwood, T. B. L., 219Kistler, H. Corby, 587Kitano, Kazuaki, 759Kitzler, Jeffrey, 346Kivaisi, Amelia, 18Klaenhammer, Todd R., 439Koch, Arthur L., 960Koide, Yoshinao, 110Kolter, Roberto, 1058Kondorosi, Adam, 881Konno, Masatoshi, 975

i

ii AUTHOR INDEX

Korch, Christopher, 231Kornacki, Jon A., 319Krauel, Verena, 1071Kroll, J. Simon, 44Krueger, Ray D., 49Krulwich, Terry A., 766Kudo, Toshiaki, 404Kumazaki, Tsutomu, 713Kunkel, Barbara, 66Kuramitsul, Howard K., 711Kuroda, Mitzi I., 792

Lacy, George H., 279Lamed, Raphael, 828Lampson, Bert C., 888Leavitt, Ron W., 467LeBlanc, Donald J., 855Lee, Kin-Sang, 1081Lee, Linda N., 855Lee, Yang, 492Lee, Young-Moo, 82LeGall, Jean, 722Lehrbach, Philip R., 447, 455Leong, Sally A., 587Leppik, R. A., 455Leung, Patrick, 82Levy, Esther, 423Lewis, Thomas A., 981Lindahl, Lasse, 1095Londei, Paola, 265Lorenz, R. Todd, 981Lorian, Victor, 492Loshon, Charles A., 168Lovett, Paul S., 842Ludden, Paul W., 999Lugtenberg, Ben, 1083Lynn, Melanie E., 940

Mason, James M., 174Matsuhashi, Michio, 975Matsumoto, Kunihiro, 713Matthews, Barbara G., 517Matzanke, Berthold F., 666,

674May, Gerhard, 433McCabe, J. B., 1066McFall, Elizabeth, 429Mclnerney, Michael J., 179McIntosh, Mark A., 375McKem, Neil M., 243McLaughlin, Jane R., 952Mermod, Nicolas, 447, 455Merryweather, Andrew, 12Meyer, Richard J., 703Michaelis, Susan, 160Miguel, Ann Gunsalus, 272Miller, Stephen P. F., 522Molineux, Ian J., 251Monod, Michel, 138Montgomery, Rex, 893Montoya, Alice L., 387Moon, Harley W., 799Moreno, Felipe, 1058Moseley, Steve L., 799Motamedi, Haideh, 575, 581Mount, Mark S., 279Moxon, E. Richard, 44Muller, Gertraud, 674Mutoh, Norihiro, 992

Nagle, James, 870Najera, Rebeca, 487Nakamura, Akira, 110

Narahashi, Yoshiko, 404Neal, Gregory, 1035Ne'eman, Zvi, 299Neilands, J. B., 350Nicas, Thalia I., 473Nichols, Peter D., 238Niederman, Robert A., 153Noda, Yasuko, 1016Normark, Staffan, 231Noti, John D., 774, 784

O'Connor, Kathleen A., 744Olivera, Hiram, 487Oosawa, Kenji, 992Oppenheim, Amos B., 415Otaka, Eiko, 713Otani, Mieko, 148Otten, Sharee, 893Ownby, James D., 940

Paoletti, Lawrence C., 73Paranchych, William, 660, 754Parisi, Joseph T., 888Park, Chankyu, 101Parker, Ann P., 719Parker, Charlotte D., 375Parker, J. M. R., 660Parks, Leo W., 981Parr, Thomas R., Jr., 473Peck, Harry D., Jr., 722Peters, Jurgen, 1048Pettis, Gregg S., 375Phelps, Patricia, 4%Pieroni, P., 660Piggot, Patrick J., 257Pirola, Angelo, 740Pittard, James, 556, 749Plesofsky-Vig, Nora, 124Podger, Denis M., 420Porter, R. D., 1074Postma, Pieter W., 393Preiss, Jack, 82Premakumar, R., 480Prickril, Benet C., 722Pritchard, Arthur E., 291Prochoda, Michael, 4%Przybyla, A. E., 722Putnoky, Peter, 881

Raab, Ronald, 1035Rabinowitz, Jesse C., 205Ramirez, Remedios, 544Ramos, Juan L., 447Raucci, Giuseppe, 77Raymond, Kenneth N., 666,

674Reddy, T. L. Prasad, 1Redlinger, T. E., 655Reilly, Patricia A., 153Reineke, W., 455Rekik, M., 455Richaud, Francois, 400Ritchie, Lyndal J., 420Roberts, Anne N., 257Roberts, Daniel P., 279Roberts, Gary P., 999Roberts, Mark, 467Robinson, Arthur C., 809Rodriguez, Russell J., 981Romano, Nino, 1089Romero, David, 1043Rosenberg, Eugene, 356Ross, Wilma, 905

Rottem, Shlomo, 299, 986,1089

Rowe, D. B., 379Ruby, E. G., 1066

Sako, Tomoyuki, 850Sanders-Loehr, Joann, 57San Millan, Jose Luis, 1058Sano, Keiji, 148Sanz, Jose Luis, 265Schaaper, Roel M., 130Schimz, Angelika, 305Schmetterer, Georg, 411Schneider, Robert A., 799Schoedel, Christianne, 117Schreier, Harold J., 35Schroeder, Craig J., 616Schwalbe, Richard S., 186,

685Schwarz, Uli, 492Scopes, Robert K., 863Segovia, Lorenzo, 487Serre, Marie-Claude, 639Setlow, Barbara, 168Setlow, Peter, 168, 174Shah, Vinod K., 999Sherman, Louis A., 396Shiba, Kiyotaka, 201Shibuya, Ichiro, 551Shimada, Akira, 508Shimotsu, Hidenori, 792Shirvan, Mitchell H., 1089Shore, Scott H., 905Short, Kevin A., 729Shuman, Henry, 935Siden, Inga, 18Sidhu, Anita, 766Signer, Ethan R., 66Silverman, Michael, 210Simon, Melvin, 210Simon, Melvin I., 992Sinskey, Anthony J., 695Sober6n-Chavez, Gloria, 487Somlyo, Andrew P., 935Sonenshein, Abraham L., 35Song, Min Dong, 975Sparling, P. Frederick, 685Spector, Michael P., 1086Speziale, Pietro, 77Spratt, Brian G., 1004Steven, Alasdair C., 968Stevens, S. E., Jr., 1074Stewart, David J., 243Stewart, Valley, 383Strauch, Mark A., 191Stromberg, Verlyn K., 279Sweeney, Janis, 8098witalski, Lech M., 77Szalay, Aladar A., 774, 784

Tadros, Monier Habib, 96Tai, Phang C., 389Takagi, Masamichi, 551Thomas, Daniel, 968Thompson, John, 522Tietz, Alisa, 356Timmis, Kenneth N., 447, 455Timpl, Rupert, 77Ting, Yi E., 952Tippett, Janet, 44Tirgari, Simin, 1086Todd, John A., 257

Toma, Salvatore, 740Tomasek, Paul H., 818Tomasz, Alexander, 759, 1077Treml, Suzanne B., 89Trus, Benes L., 968Tsai, Lin, 681Tuomanen, Elaine, 535, 759,

1077Turken, Arthur N., 774

Ubukata, Kimiko, 975Ugalde, Rodolfo A., 947Umezawa, Chisae, 148Uozumi, Takeshi, 110

Van Arsdell, Janelle N., 952Van Den Bosch, Han, 407van de Putte, Pieter, 503van der Helm, Dick, 1020Vandeyar, Mark A., 530Van Die, Lrma, 407Van Megen, Ingrid, 407Varon, Mazal, 356Vary, Patricia S., 716Vasil, Michael L., 291Villarejo, Merna, 433Villaverde, Antonio, 1055Visai, Livia, 77

Wachi, Masaaki, 975Wake, R. G., 379Waters, Virginia L., 647Weber, Morton M., 1Weisburg, W. G., 570Wendt-Pienkowski, Evelyn,

575, 581Whalley, J. B., 219Whitehead, Terence R., 205Wild, J. R., 285Wilkins, Brian M., 12Willetts, Neil S., 754Williams, Peter H., 467Williams, Shericca, 387Winkelman, Jeffrey W., 362Winkelmann, Gunther, 1020Winkler, Herbert H., 805Winter, Greg, 257Woese, C. R., 570Wofford, Neil Q., 179Wolf, Heinz, 265Wolf, Julie B., 623Wolk, C. Peter, 411Wong, Hing C., 952Wong, W. K. Raymond, 604Woodruff, Wendy A., 473Worobec, E. A., 660Wu, W. H., 1025

Yamashita, Naoko, 975Yang, T.-S., 674Yano, Keiji, 551Yanofsky, Charles, 383, 792Young, Ry, 1035Yue, Steven, 581Yun, Allen C., 784Yura, Takashi, 201

Zachariou, Michael, 863Zahler, Stanley A., 530Zengel, Janice M., 1095Zhang, Jia Ming, 968Zorreguieta, Angeles, 947Zuidweg, Elly, 407Zusman, David R., 744Zylicz, Maciej, 25

J. BACTERIOL.

SUBJECT INDEXVOLUME 167

ADPglucose pyrophosphorylaseE. coli glgC mutant

altered allosteric properties, 82cloning and expression, 82

AerobactinE. coli ColV-K30

iron transport system, 350iucA and iucC genes, 350

Aerobactin biosynthesisColV-K30 plasmid

50,000-dalton polypeptide, role, 467E. coli iron uptake system, 467

Aerobactin iron uptake systemCoIV plasmidDNA environment, 647genes, 647

Aerobic bacteriairon respiration-driven proton transloca-

tion, 729AerolysinA. hydrophila aer gene

cloning in E. coli, 368Aeromonas hydrophila

aer gene, 368cloning in E. coli, 368

Agrobacterium spp.T-DNA tms gene products

sustained ethylene production, 387transformed carrot disks, 387

Agrobacterium tumefaciens,-D(1-2) glucan synthesis

internal-membrane enzymes, 947235-kilodalton protein intermediate,947

R. meliloti megaplasmidnodulation, 66root hair curling, 66

Ti plasmid noc and occ lociproline biosynthesis, 732

Alkaline phosphatase exportE. coli signal sequence mutants, 160

AlkalophilesBacillus spp., 766

Amino acidsS. lactismajor constituents, 522new amino acid, 522

Anabaena variabilisheterocyst gene expressionDNA-RNA hybridization, 940heterocyst RNA-vegetative-cell RNA

hybridization, 940Anaerobically regulated genes

E. coli, 362Anguibactin

V. anguillarumnovel siderophore, 57

Anthracycline antibiotic productionS. glaucescensnew metabolites, 581nonproducing mutants, 575, 581

Antibiotic resistanceB. thuringiensis phage-resistant

mutants, 18S. faecalis

bifunctional 6'-aminoglycoside acetyl-transferase 2"-aminoglycoside phos-photransferase gene, 631

Antibiotic sensitivityarchaebacterial elongation factors, 265

Antibiotic synthesis

microcin B17gene transcription regulation, 1058mcb gene transcription, 1058

Aquaspirillum magnetotacticumhydroxamate production

iron concentration, effect, 73iron uptake, 73

Aquaspirillum serpenssurface layer protein

diverse morphology, 1025three-dimensional structure, 1025

Archaebacteriaelongation factor-targeted drugs

fusidic acid, 265kirromycin, 265pulvomycin, 265

eubacterial elongation factor Tu, 265eucaryotic elongation factor 1, 265M. barkeri membrane-bound ATPase,

837polypeptide elongation factorsaminoacyl-tRNA binding to

ribosomes, 265antibiotic sensitivity, 265

Arthrobacter spp.xanthone biodegradation

catabolic intermediates, 818complete gentisate pathway, 8183,4-dihydroxyxanthone ring cleavage,

818Aspartate transcarbamoylase polypeptides

Enterobacteriaceaein vivo formation, 285pyrB catalytic gene, 285pyrI regulatory gene, 285

ATP-dependent protein translocationE. coliH+-ATPase, 389proton motive force, 389

ATP hydrolysisE. coliRecA and LexA protein levels, 1055SOS induction, 1055UV-light effects, 1055

ATP transport systemB. bacteriovorus

active transport permease, 1066intact ATP transport, 1066

Autocide AMIM. xanthus

fatty acid composition, 356fruiting body formation defects, 356resistance mutants, 356

AutolysisE. coli

cell wall degradation products, 759E. coli relA + cells

induction, 1077resistance, 1077

Auxin synthesisAgrobacterium spp., 387

Azobacter vinelandiiiron-molybdenum nitrogenase cofactor

in vitro synthesis, 999nifV-dependent factor, 999nitrogen fixation-derepressed cultures,999

nitrogen fixationmolybdenum transcriptional regula-

tion, 480nifH-homologous regions, 480

Azospirillum brasilensenif expression regulation, 423

histidine transport regulation, 423nitrogen fixation, 423

Bacillus cereusrespiratory systems

composition, 544forespore, 544mother cell, 544sporulation stages, 544

small, acid-soluble spore protein genes,168

Bacillus Genetic Stock CenterB. subtilis Tn9J7 insertion mutants, 530

Bacillus marine speciesvegetative cellscytochromes b and c, 30electron transport system, 30manganese reduction, 30

Bacillus megateriumcobalamin biosynthesis genes, cloning,

623penicillin acylase genes, 623Tn9J7 transposition, 716vitamin B12 genes, cloning, 623

Bacillus pumilustrp operon regulatory elements

B. subtilis trp, comparison, 792trans-acting transcription termination

factor, 792Bacillus spp.

cell wall teichoic acidscomparative studies, 508structure, 508

facultative alkalophilic strainsalkaline and neutral pH effects, 766isolation and characterization, 766

lipoteichoic acidsBacillus stearothermophilus

small, acid-soluble spore protein genes,168

Bacillus subtiliscat-86 induction, 842cell growth kinetics

nucleoid extension correlation, 219surface growth rates, 219

chloramphenicol acetyltransferase geneamicetin induction, 842cat-86, 842chloramphenicol induction, 842regulatory sequences, 842

cytochrome b558amplification in E. coli membranes,

735electron paramagnetic resonance

spectroscopy, 735membrane succinate dehydrogenasecomplex, 735

endospore heat resistance, 257glutamine synthetase mutants

P-galactosidase derepression, 35gInA gene regulation, 35

intracellular serine protease genecloning and sequencing, 110sporulation, 110

macrolide-lincosamide-streptogramin Bresistance plasmid, 138

mutant sporesheat resistance reduction, 257

neutral protease expression

iii

iv SUBJECT INDEX

nprR regulatory regions, 740transcript accumulation effects, 740

penicillin-binding protein 5 genecloning in E. coli, 257

phage SP16 genomecircular permutation, 719terminal redundancy, 719

plasmid pIM13sequence and properties, 138

prophage SP, homology region, 379replication terminus terCnonrandom cosmid cloning, 379prophage SP,B homology, 379

spore UV-light resistancesmall, acid-soluble spore proteins, 174

succinate dehydrogenase complexcytochrome b558 amplification, 735

temperate phage SP, d geneimmunity control, 952lysogeny control, 952

Tn9O7 chromosomal insertionsauxotrophic requirements, 530cryptic mutations, 530location and characterization, 530

trp operon regulatory elementsB. pumilus trp, comparison, 792

Bacillus thuringiensisphage-resistant mutants

antibiotic resistance, 18decreased virulence, 18extracellular protein production, 18flagellation, 18H. cecropia pupae, 18hemolymph susceptibility, 18

Bacterial cell surfaceshydrophobicity determination

nonionic-surfactant adsorption, 1016Bacterial conjugation

plasmid RP4-encoded DNA primase, 12Bacteriochlorophyll

C. aurantiacusphotosynthetic membrane system, 655

BacteriocinL. helveticus helveticin J, 439

BacteriophageBacillus phage SP16 genome

circular permutation, 719terminal redundancy, 719

B. subtilis phage SP,3 d geneimmunity control, 952lysogeny control, 952

F-like pilin-binding sites, 660lambda clII gene

lysogenic response, 415tor mutations, 415translational regulatory signals, 415

lambda DNA replicationE. coli grpE gene, 25heat shock protein, 25temperature effects, 25

lambda immP22 growth inhibitionE. coli sip gene mutations, 191phage P22 cl gene product, 191

lambda lysis gene Sloss of alpha-helical character, 1035mutational analysis, 1035protein oligomerization, 1035

lambda Q gene product, 429Mu

E. coli replication-defective mutants,905

Mu transpositiongene c transcription, 503

Ner- mutant, 503Ner protein role, 503plaque formation, 503

Mx4M. xanthus genetic analysis, 744

P1M. xanthus genetic analysis, 744

T7S. sonnei infection, 251

T3 DNA ligaseS. sonnei DNA repair, 251

T5 Itf genecloning and expression, 1071polypeptide identification, 1071Tnl000 insertion analyses, 1071unique 5-kilobase BamHI fragment,

1071Bacteroides nodosus

two-subunit pilin genenucleotide sequence, 243

Bacteroides spp.R plasmid pCP1

clindamycin resistance regions, 517cloning in E. coli, 517replication function region, 517tetracycline resistance regions, 517

Bdellovibrio bacteriovorusATP transport system

active transport permease, 1066intact ATP transport, 1066

Benzyl alcohol oxidationTOL plasmid pWWO, 455

Bifunctional 6'-aminoglycoside acetyltrans-ferase 2"-aminoglycoside phosphotrans-feraseS. faecalis gene

antibiotic resistance, 631cloned regions, 631DNA sequence analysis, 631

Bordetella pertussisfimbriae

density, 968helical structure, 968phase variations, %8

Bradyrhizobium japonicumnitrogen fixation genes

,-galactosidase expression, 784nif promoter-lacZ fusion studies, 784

symbiotic nitrogen fixationfix gene regions, 774nif gene transcription and translation,

774nitrogenase genes, isolation, 774

Broad-host-range expression vector, 447

Candida maltosahost-vector system, constructionARS site, 551n-alkane assimilation genes, 551organelle biogenesis genes, 551

Capsule productionH. influenzae type b capsule genes, 44

Carotenoid-binding proteinSynechocystis cytoplasmic membrane,

396Cell envelope-cell division gene cluster

E. coli ddlftsQ regionoverlapping transcriptional elements,

809Cell growth kinetics

B. subtilisnucleoid extension correlation, 219surface growth rates, 219

Cell mass-cell volume relationshipS. faecium RNA and protein syntheses,960

Cell septationE. coliPBP3-RodA protein interaction, 1004rodA gene, 1004

Cell shapeE. coli, 1004

Cellulase gene expressionR. capsulatus

endoglucanase synthesis, 604exoglucanase synthesis, 604host culture aeration, 604plasmid expression vectors, 604

CellulosomeC. thermocellum cell surface

cellulose interaction, 828ultrastructure, 828

Cell wall autolytic degradationE. coli, 759

Cell wall synthesisinhibitors

antibacterial effects, 535synthetic enzymes

cell growth rates, 535functional versus nonfunctional en-

zymes, 535penicillin-binding proteins, 535

Chemosensory transducerE. coli Trg proteins, 101

ChemotaxisE. coli receptor mutants, 992

Chlamydiaeeubacterial origin

cell wall peptidoglycan content, 570relationship to planctomyces, 570rRNA sequence comparisons, 570

Chloramphenicol acetyltransferase geneB. subtilis cat-86

amicetin induction, 842chloramphenicol induction, 842regulatory sequences, 842

Chloroflexus aurantiacusphotosynthetic membrane system

bacteriochlorophyll effects, 655cytochrome content, 655light effects, 655oxygen developmental regulation, 655

Cholesterol uptakeS. cerevisiae, 981

Chromatophore membrane formationR. rubrumcytoplasmic-membrane invagination,

89immunocytochemical ultrastructural

analysis, 89Chromatophores

R. capsulatacytoplasmic surface, 96light-harvesting polypeptides, 96

cis-Acting proteinsDNA replicative proteins, 429A Q gene product, 429D-serine deaminase activator protein,429

transposases, 429Clostridium acidiurici

10-formyltetrahydrofolate synthetasegeneE. coli cloning and expression, 205

Clostridium thermocellumcell surface cellulosome

J. BACTERIOL.

SUBJECT INDEX vVOL. 167, 1986

cellulose interaction, 828ultrastructure, 828

Cobalamin biosynthesisB. megaterium genes, 623

Collagen bindingS. aureus

bacterial tissue colonization, 77cell surface receptor, 77fibrinogen effect, 77structural requirements, 77

ConidiosporesN. crassa

cycloheximide treatment, 124freezing injury, 124protection by heat shock, 124

Corynebacterium glutamicumpheA gene

chorismate mutase activity, 695cloning and sequencing, 695E. coli pheA auxotroph complementa-

tion, 695prephenate dehydratase activity, 695

CyanobacteriaA. variabilis

heterocyst gene expression, 940heterospecific transformation, 1074luciferase expression

V. fischeri, 411V. harveyi, 411

physiological transformation, 1074Synechococcus or Synechocystis spp.,

1074Synechocystis sp.

carotenoid-binding protein, 396Cyclic AMP

S. typhimuriumreceptor protein genes, 616receptor protein interaction, 616

Cyclic-AMP-dependent protein kinaseS. cerevisiae ribosomal protein phos-

phorylation, 713Cyclic diphosphoglycerate accumulationM. thermoautotrophicum, 49

Cytochrome b558B. subtilis succinate dehydrogenase

complex, 735electron paramagnetic resonance spec-

troscopy, 735Cytochromes

Bacillus sp. vegetative cells, 30B. cereus respiratory systems, 544

Deinococcus radioduranssurface-layer protein gene

cloning in E. coli, 1048HPI polypeptide expression, 1048

De Ley-Doudoroff pathwayR. meliloti galactose metabolism, 1092

Desulfovibrio vulgarisperiplasmic hydrogenase, small subunit

putative signal peptide, 722Differential methylation

E. coli chemotaxis receptor mutants,992

Disaturated phosphatidylcholinesMycoplasma species, 299Spiroplasma species, 299

DNA adenine methylationS. typhimurium mutant

frameshift mutation induction, 420DNA-membrane complex

plasmid RK2 replication, 319DNA polymerase III

E. colilacI mutation frequencies, 130mutD5 mutational specificity, 130

DNA primaseplasmid RP4

bacterial conjugation, 12DNA replicative proteins, 429D-Serine deaminase activator protein, 429

Electron paramagnetic resonance spec-troscopyB. subtilis cytochrome b558, 735

Electron transport systemBacillus sp. vegetative cellsmanganese reduction, 30

Elongation factorsarchaebacteria

antibiotic sensitivity, 265eubacterial factors, 265eucaryotic factors, 265factor-targeted drugs, 265

Endospore heat resistanceB. subtilis

penicillin-binding protein 5, 257Enterobacteriaceae

hybrid aspartate transcarbamoylasesin vivo formation, 285pyrB catalytic characteristics, 285pyrI regulatory control, 285

Enzyme 111G1c

E. coli glycerol kinase regulation, 393Erwinia carotovora

pectolytic enzymescellulase, 279E. coli potato maceration, 279endopectate lyase, 279endopolygalacturonase, 279exopectate lyase, 279

Erwinia chrysanthemipectate lyase pelB gene

regulatory mutants, 400pelB-pelC gene homology, 117pectate lyase genes, homology, 117

Erwinia herbicolacell-free ice nuclei, 496ice nuclei, release, 496outer membrane vesicles, 496

Escherichia coliA. hydrophila aer gene cloning, 368alkaline phosphatase export

signal sequence mutations, effects,160

anaerobically induced genesmap locations, 362phenotypes, 362regulatory properties, 362

aroP genecloning, 749product identification, 749

asparagine metabolism mutants, 362aspartate metabolism mutants, 362ATP-dependent protein translocationH+-ATPase, 389proton motive force, 389

ATP hydrolysisRecA and LexA protein levels, 1055SOS induction, 1055UV-light effects, 1055

autolysiscell wall degradation products, 759murein degradation, 759

autolysis inductionnongrowing cells, 1077

resistance, 1077Bacteroides plasmid cloning, 517btuCED genes

nucleotide sequence, 928periplasmic binding protein transport

system, homology, 928vitamin B12 transport, 920, 928

C. acidiurici 10-formyltetrahydrofolatesynthetase genecloning and expression, 205

calcium content, 935cell envelope

penicillin-binding site, 492cell envelope-cell division gene cluster

overlapping transcriptional units, 809cell physiologyMalE-LacZ protein effects, 201

cell shape and division requirementsprotein PBP3-RodA interaction, 1004

cell wall synthesis inhibitors, 1077chemotaxis receptor mutants

differential methylation, 992nonsense and missense mutations,

992null phenotypes, 992signaling defect, 992tar mutation, 992Tar protein, 992

chorismate mutase-P-prephenatedehydratase, 695

ColV-K30 aerobactin systemaerobactin biosynthesis, 350iucA and iucC genes, 350

ColV-K30 plasmidaerobactin biosynthesis, 467iron-uptake system, 467

crp geneS. typhimurium crp sequence homol-

ogy, 616crp gene comparisons

S. flexneri crp, 639S. typhimurium crp, 639

dam mutants, 420ddlftsQ regionDNA sequence, 809overlapping transcriptional units, 809transcriptional organization, 809

DNA polymerase III3'-*5'exonuclease, 130replication fidelity, 130

F41 adhesinDNA cloning, 799homology with K88 adhesin, 799

ferric enterobactinouter membrane protein receptor,

666, 674recognition and transport, 666, 674

fimbriaefunctional relationship of gene clus-

ters, 407serological differences, 407

ftsl genecell septation, 1004

glgC mutant geneADPglucose pyrophosphorylase mu-

tant, 82cloning and expression, 82

glycerol kinase regulationenzyme IlGlIc, 393

grpE heat shock protein geneA DNA replication, 25host growth, 25temperature effects, 25

vi SUBJECT INDEX

htpR gene function, 201iron enterobactin uptake

cytoplasmic uptake, 674ferric enterobactin siderophore com-

plex, 674Mossbauer spectroscopic monitoring,

674periplasmic uptake, 674

LexA protein, 1055linear plasmid multimers

recB21 recC22 sbcB15 mutant, 327recombination frequencies, 327rolling-circle-DNA synthesis initia-

tion, 327log-phase anid dividing cells

calcium content, 935elemental composition, 935magnesium content, 935monovalent Na, Cl, and K ions, 935

magnesium content, 935MalE-LacZ protein effects

envelope protein export, 201heat shock protein induction, 201

maltose-binding protein-p-galactosidasehybrid, 201

mannose-resistant hemagglutination, 799monovalent ions, 935murein degradationendopeptidase participation, 759transglycosylase participation, 759

mutDS mutational specificityDNA polymerase III activity, 1303'-*5'exonuclease defect, 130lacI mutation frequencies, 130

N-acetylneuraminate lyase gene, 404nanA, 404osmotic stress adaptation

envelope protein composition, 433glycine betaine regulation, 433proU induction, 433

outer membrane ferric enterobactin re-ceptorbinding specificity, 666, 674fepA, 666

outer membraneslipopolysaccharide 0-antigen, effects,

1081P. aeruginosa hemolysin gene cloning,

291P. aeruginosa porin protein F gene ex-

pression, 473paraquat lethality

effect of 2,4-dinitrophenol, 346effects of salts, 346uptake-defective mutant, 346

penicillin-binding proteinspH effects, 492

periplasm, 160phage Mu replication-defective mutants

gyr mutations, 905himA mutations, 905hip mutations, 905

phage Mu replicative transpositions, 905pheA auxotroph complementation

C. glutamicum pheA, 695phoA mutations, 160plasmid R1162 conjugative mobilization

oriT requirement, 703potato tissue maceration

E. carotovora pectolytic enzymes,279

poxB mutants, 312pyruvate oxidase mutants

cloning, sequencing, and analyses,312

lipid activation defects, 312recA genehomology with V. cholerae recA, 375

RecA protein, 1055recD gene

characterization, 594exonuclease V activity, 594plasmid maintenance, 594recombination, 594

relA+ cellsamino acid starvation, 1077autolysis induction, 1077

ribosomal gene transcriptionnutritional shift-up, 1095S10 transcription attenuation mecha-

nism, 1095rodA gene

cell shape, 1004sak product export

cytoplasmic membrane, 850immediate export pathway entrance,

850secretory proteins, transportjamming phenomenon, 462translation and sorting, 462

sip gene mutationsA imm'22 inhibition, 191phage P22 ci gene product, 191

SOS inductionATP hydrolysis, 1055

staphylokinase exportpathway entrance requirement, 850

S. typhimurium GlpQ' proteinE. coli protein transport jamming, 462

Tn917 transpositionF' plasmid derivative, 711shuttle plasmid pHK1207, 711

trg mutationsligand binding, 101Trg chemosensory transducer, 101

Trp repressor proteinintracellular levels, 272trpR autoregulatory gene, 272L-tryptophan effects, 272

trpR gene, 272tryptophanase (tna) operon expression

leader peptide synthesis, role, 383tyrP gene

expression regulation, 556promoter operator region, 556tyrosine-mediated repression, 556

vitamin B12 transportBtuCED polypeptides, role, 920, 928cytoplasmic-membrane-transport

genes, 920, 928outer-membrane-transport genes, 920

Ethylene productionAgrobacterium-transformed carrot disks

elevated auxin synthesis, 387T-DNA tms gene expression, 387

EubacteriaC. psittaci relatedness

cell wall peptidoglycan content, 570rRNA sequence comparisons, 570

Exfoliative toxin BS. aureus gene sequence, 726

F41 adhesinE. coliDNA cloning, 799homology with K88 adhesin, 799

Fatty acid compositionM. capricolum, 986M. capsulatusgrowth at low oxygen tensions, 238

Fatty acid metabolismS. wolfei

cell-free extracts, 179enzymes, 179

Ferric enterobactinE. coli

outer membrane receptor specificity,666, 674

recognition and transport, 666, 674Fimbriae

B. pertussisdensity, 968helical structure, 968phase variations, 968

E. colifunctionally related gene clusters, 407serological differences, 407

FlagellaB. thuringiensis phage-resistant

mutants, 18V. parahaemolyticus laf genesurface-dependent expression, 210transcription regulation, 210

FlavoproteinM. phlei succinate dehydrogenase, 1

F-like piliantibody-binding locations, 660antigenic determinants, location, 660phage-binding locations, 660protein A-gold immunoelectron micros-

copy, 66010-Formyltetrahydrofolate synthetase

C. acidiurici geneE. coli cloning and expression, 205

Frameshift mutationsS. typhimurium, 420

Freezing injuryN. crassa conidiospores

cycloheximide treatment, 124protection by heat shock, 124

Fruiting body formationM. xanthus

autocide AMI, 356fatty acid composition, 356

Fusarium oxysporumlinear plasmidlike DNA

exonuclease sensitivity, 587restriction endonuclease mapping, 587

Galactose metabolismR. melilotiDe Ley-Doudoroff pathway, 1092

3-GalactosidaseB. japonicum nitrogen fixation genes,

effects, 784B. subtilis glutamine synthetase mu-

tants, 35E. coli cell physiology, 201

P-D(1-2) Glucan synthesisA. tumefaciens, 947R. meliloti, 947

Gluconate formationB. megaterium spore germination, 149

Glucose acquisitionR. prowazekii

uridine 5'-diphosphoglucose trans-port, 805

Glucose catabolismB. megaterium spore germination, 149

J. BACTERIOL.

SUBJECT INDEX vii

Glucose-fructose oxidoreductaseZ. mobilis sorbitol production, 863

Glutamate synthaseN. crassa structural gene

en(am)-2 mutations, 1043Glutamine synthetase

B. subtilis mutants, 35Glycerol kinase regulation

E. coli enzyme IIIGlC, 393Glycine betaine

E. coli osmotic-stress adaptation, 433Gonococci

cryptic-plasmid transfer, 685Gram-negative bacteria

broad-host-range expression vector, 447

Haemophilus influenzaetype b capsule genes

capsule production, 44deletion mutagenesis, 44virulence, 44

Halobacterium halobiumphotosensory signals

attractant or repellent response, 305integration steps, 305oscillator shape changes, 305swimming direction, 305

Heat resistanceB. subtilis endospores, 257

Heat shock proteinE. coli grpE gene, 25

Hemolymph susceptibilityB. thuringiensis phage-resistant

mutants, 18Hemolysin

P. aeruginosa genecloning in E. coli, 291phosphate regulation, 291

HeterocystsA. variabilis gene expression, 940nitrogen fixation sites, 940

Hfr matingV. cholerae, 375

Histidine transport regulationA. brasilense, 423

HPI polypeptideD. radiodurans surface-layer protein

gene, 1048Hyalophora cecropia pupae

B. thuringiensis avirulent mutants, 18Hydrophobicity

bacterial cell surfacesdetermination by nonionic

surfactants, 1016Hydroxamate productionA. magnetotacticum

iron concentration effect, 73iron uptake, 73

P. aeruginosa mutants, 7

Ice nucleicell-free nuclei

outer membrane vesicles, 496protein-modifying reagents, 496

E. herbicola, 496Pseudomonas spp., 496shedding in growth medium

E. herbicola, 496P. fluorescens, 496P. syringae, 496

Immunocytochemical ultrastructural anal-ysisR. rubrum chromatophore membrane,

89Immunoglobulin G-binding protein

Streptococcus sp. spg geneexpression in E. coli, 870nucleotide sequence, 870

Intracellular serine proteaseB. subtilis, 110

Intragenic variationN. gonorrhoeae

cryptic-plasmid DNA, 231site-specific recombination, 231

Iron-molybdenum nitrogenase cofactorA. vinelandii, 999K. pneumoniae, 999

Iron respiration-driven proton transloca-tion, aerobic bacteria, 729

Iron uptakeA. magnetotacticum

hydroxamate role, 73Iron uptake system

E. coli aerobactin biosynthesis, 467

Klebsiella pneumoniaeiron-molybdenum nitrogenase cofactor

in vitro synthesis, 999nifV-dependent factor, 999nitrogen fixation-derepressed cultures,999

,-Lactam antibioticsS. aureus penicillin-binding-protein genetobramycin resistance, 975

S. faeciumcell mass, 562envelope growth sites, 562

Lactobacillus helveticusbacteriocin helveticin J

characterization and purification, 439chromosome determination, 439host immunity determinants, 439inhibitory spectrum, 439

Lactose-metabolic genesS. cremoris

expression by chromosomal integra-tion, 855

Lac' DNA transformation, 855Streptococcus spp. Lac + DNA homol-

ogy, 855LamB transport

E. coliS. typhimurium glpQ product, effect,

462Leader peptide synthesis

E. coli tryptophanase operon expres-sion, 383

Legionella pneumophilalipopolysaccharides

core structure, 893serospecific antigens, 893unusual lipid A, 893

serospecific antigenschemical composition, 893immunological properties, 893molecular heterogeneity, 893

Ligand interactionE. coli, 101

Light-harvesting polypeptidesR. capsulata chromatophore surface, 96

Linear plasmidlike DNAF. oxysporum, 587

Lipid interconversionsaged M. capricolum cultures

diphosphatidylglycerol/phosphatidylgly-

cerol ratio, 986phospholipid/protein ratio, 986unsaturated-/saturated-fatty acid ratio,986

Lipopolysaccharide O-Antigenhigh-molecular-weight maltosaccharides

E. coli outer membranes, 1081S. typhimurium outer membranes,

1081maltosporin binding properties

E. coli outer membranes, 1081Lipopolysaccharides

L. pneumophila serospecific antigens,893

LuminescenceV. parahaemolyticus, 210

Lysogenic responsek clll gene, 415

Macrolide-lincosamide-streptogramin BB. subtilis resistance plasmid pIM13,

138S. epidermidis resistance plasmid, 888

Maltose-binding proteinE. coli cell physiology, 201E. coli transport

S. typhimurium glpQ product, effect,462

Manganese reductionBacillus sp. vegetative cells, 30

Mannose-resistant hemagglutinationE. coli, 799

Membrane-bound ATPaseM. barkerii

characterization and purification, 837divalent-cation requirements, 837eubacterial FoF, ATPase, similarity,

837optimum pH, 837

Membrane fraction compositionB. cereus respiratory systems, 544

Membrane lipid compositionM. capricolum

cell volume effects, 1089cholesterol/phospholipid ratio, 1089

Membrane lipid synthesisM. capsulatusgrowth at low oxygen tensions, 238

Membrane transportN. crassa

iron-surrounding N-acyl residues,1020

molecular recognition ofsiderophores, 1020

peptide backbone, 1020Methanobacterium thermoautotrophicum

cyclic diphosphoglycerate accumulation,49

phosphate-limited growth, 49phosphate uptake, 49

Methanococcus vannieliinitrogen source

purines, 681pyrimidines, 681

Methanosarcina barkerimembrane-bound ATPase

characterization and purification, 837divalent-cation requirements, 837eubacterial FoF1 ATPase, similarity,

837optimum pH, 837

Methyl methanesulfonate resistanceV. cholerae recA gene, 375

VOL. 167, 1986

viii SUBJECT INDEX

Methylococcus capsulatusgrowth at low oxygen tensions

cyclopropane fatty acid increase, 238membrane lipid changes, 238methyl sterol increase, 238

intracytoplasmic membrane systems,238

Methyl sterol compositionM. capsulatusgrowth at low oxygen tensions, 238

Microcin B17 genestranscription regulationgrowth phase, 1058ompR gene product, 1058

MolybdenumA. vinelandii nifH gene

transcriptional regulation, 480Mossbauer spectroscopy

E. coli iron enterobactin uptake, 674Murein degradation products

E. coli autolysis, 759Mycobacterium phleimembranes, 1succinate dehydrogenase

purification, 1solubilization, 1

Mycoplasma capricolumlipid interconversions

aging cultures, 986diphosphatidylglycerol/phosphatidylglycerc

ratio, 986phospholipid/protein ratio, 986unsaturated-/saturated-fatty acid ratio,986

membrane lipid compositioncell volume effects, 1089cholesterol/phospholipid ratio, 1089

Mycoplasma spp.disaturated phosphatidylcholines

fatty acid concentration, effects, 299incorporation and modification, 299intracytoplasmic-membrane associa-

tion, 299Myxococcus xanthus

autocide AMIfatty acid composition, 356fruiting body formation defects, 356resistance mutants, 356

genetic analysis, 744isolation of gene replacementsphage Mx4, 744phage P1, 744

N-Acetylneuraminate lyaseE. coli

constitutive synthesis, 404nanA gene, 404

n-Alkane assimilation genesC. maltosaARS site, 551host-vector system, 551

N5-(1-carboxyethyl)-ornithineS. lactis intracellular amino acid, 522

Neisseria gonorrhoeaecryptic-plasmid DNA

intragenic variation, 231site-specific recombination, 231

cryptic-plasmid presence and transfer,685

isogenic derivatives, 685protein II genes

genetic transformation, 186outer membrane proteins, 186

repetitive sequencesantigenic variation, 1009phase variation, 1009two-dimensional heteroduplex map-

ping, 1009Neisseria meningitidis

repetitive sequences, 1009Neurospora crassa

conidiospore freezing injurycycloheximide treatment, 124protection by heat shock, 124

glutamate synthase structural geneen(am)-2 mutations, 1043

membrane transportiron-surrounding N-acyl residues,

1020peptide backbone, 1020

molecular recognition of siderophores,1020

Neutral protease expressionB. subtilisnprR regulatory regions, 740

Nitrogenase genesB. japonicum, 774

Nitrogen fixationA. brasilense, 423A. variabilis heterocysts, 940A. vinelandii nifHDK gene clustermolybdenum transcriptional regula-

l tion, 480nifI fragment-homologous regions,480

B. japonicumfix gene regions, 774nif genes, 774nitrogenase genes, 774, 784

Nitrogen sourceM. vannielii

purines, 681pyrimidines, 681

NodulationR. meliloti nod genes

early essential functions, 881nodulation efficiency, 881

R. phaseolimodification by genetic rearrange-

ments, 487

Obligate alkalophilyBacillus alkalophilic strains

alkaline and neutral pH, 766physiological comparisons, 766

Osmotic stressE. coli adaptation, 433

Outer membrane protein II genesN. gonorrhoeae, 186

Outer membrane vesiclescell-free ice nuclei, shedding

E. herbicola, 496P. fluorescens, 496P. syringae, 496

OxidoreductasesB. cereus respiratory systems, 544

Paraquat lethalityE. coli

effect of 2,4-dinitrophenol, 346effects of salts, 346uptake-defective mutant, 346

Pectate lyaseE. chrysanthemi pelB mutants, 400

Pectate lyase isozymes

E. chrysanthemipelB-pelC gene homology, 117purification and properties, 117

Pectolytic enzymesE. carotovora genes

E. coli potato tissue maceration, 279Penicillin acylase

B. megaterium genes, 623Penicillin-binding protein

E. coli cell envelope sitepH effects, 492reactions with penicillin, 492

S. aureus gene1-lactam antibiotic resistance, 975molecular cloning, 975tobramycin resistance, 975

Penicillin-binding protein SB. subtilisdacA cloning in E. coli, 257endospore heat resistance, 257

Penicillin-binding proteinscell wall-synthesizing enzymes

acylation and cell lysis, 535short functional half-life, 535

Periplasmic binding-protein-dependenttransport systemsE. coli btuCED genes

vitamin B12 transport system homol-ogy, 928

Periplasmic hydrogenaseD. vulgaris

putative signal peptide, 722small subunit, 722

Phosphate uptakeM. thermoautotrophicum, 49

Phosphoenolpyruvate:carbohydrate phos-photransferase systemE. colienzyme IIIGlc, 393glycerol kinase regulation, 393

Phospholipase CP. aeruginosa gene

cloning in E. coli, 291sequence and expression, 291

Phospholipid contentM. capricolum, 986

Phosphomannose isomeraseE. coli expression, 611P. aeruginosa expression, 611

Phosphorylation reaction enzymesS. wolfei, 179

Photosensory signalsH. halobium

attractant or repellent response, 305integration steps, 305oscillator shape changes, 305swimming direction, 305

Photosynthetic membrane developmentR. sphaeroides synchronously dividing

cells, 153Photosynthetic membrane system

C. aurantiacusdevelopmental factors, 655

PilinsB. nodosus two-subunit gene

nucleotide sequence, 243Plaque formationphage Mugene c transcription, 503Ner protein role, 503transposition, 503

Plasmidsaerobactin iron uptake system

J. BACTERIOL.

SUBJECT INDEX ix

DNA environment, 647genes, 647

A. tumefaciens Ti plasmidproline biosynthesis, 732

Bacteroides plasmid pCP1cloning in E. coli, 517

broad-host-range expression vector, 447B. subtilis pIM13

sequence and properties, 138ColV, 647ColV-K30

aerobactin gene cluster transcription,467

aerobactin iron transport system, 350iron uptake system, 467iucA and iucC genes, 350RNA-DNA hybridization analysis,

467gonococcal cryptic-plasmid transfer, 685IncF plasmidsfinP alleles, 754finP RNA molecule, 754

IncP groupRP4-encoded DNA primase, 12

linear multimersE. coli mutants, 327rolling-circle-DNA synthesis, 327

linear plasmidlike DNAF. oxysporum, 587

pBR322phage T5 ltf gene cloning, 1071

R1162 DNAconjugative-plasmid mobilization, 703genetic organization, 703oriT involvement, 703

RK2 replicationcoupling to transcription and transla-

tion, 319DNA-membrane complex, 319trfA-encoded protein, 319

R. meliloti megaplasmidexopolysaccharide loci, 66thiamine loci, 66

RP4-encoded DNA primase, 12R. phaseoli symbiotic plasmid

genetic rearrangements, 487nodulation modification, 487

R100 traM and finP genes, 336S. epidermidis macrolide-lincosamide-

streptogramin B resistance plasmidpNE131nucleotide sequence, 888S. aureus plasmid homology, 888

stable maintenanceE. coli recD gene, 594

Tn917 transpositionF' plasmid derivative, 711shuttle plasmid pHK1207, 711

TOL plasmid pWWOP. putida upper pathway operon, 455xylA, 455

Porin protein FP. aeruginosa gene

expression in E. coli, 473Potato tissue maceration

E. coliE. carotovora pectolytic gene prod-

ucts, 279Proline biosynthesisA. tumefaciens Ti plasmid

noc and occ loci, 732Prophage SPP homology region

B. subtilis terC, 379

Protein A-gold immunoelectron micros-copyF-like pili, 660

Protein oligomerizationX lysis gene S

loss of alpha-helical character, 1035mutational analysis, 1035

Proton motive forceE. coli ATP-dependent protein

translocation, 389Proton translocation

aerobic bacteriairon respiration effect, 729

Pseudomonas aeruginosaalgA transcriptionphosphomannose isomerase expres-

sion, 611tac promoter control, 611

hemolysin genecloning in E. coli, 291phosphate regulation, 291

phospholipase C genecloning in E. coli, 291sequence and expression, 291

phosphomannose isomeraseoverproduction and assay, 611

porin protein F geneexpression in E. coli, 473outer membrane protein, 473

pyoverdin mutationshydroxamate production, 7map positions, 7nonfluorescence, 7temperature sensitivity, 7

Pseudomonas putidaTOL plasmid pWWO

oxidative catabolism, 455upper pathway operon, 455xylA, 455

xylA producttoluene and benzyl alcohol oxidation,

455Pseudomonas spp.

cell-free ice nuclei, 496ice nuclei release, 496outer membrane vesicles, 496

Purine metabolismM. vannielii, 681

Pyoverdin productionP. aeruginosa mutants, 7

Pyrimidine metabolismM. vannielii, 681

Pyruvate oxidase mutantsE. coli poxBDNA and enzymatic analyses, 312lipid activation, 312

Rhizobium leguminosarumfractionation of cells

cytoplasmic components, 1083outer membrane, 1083periplasmic membrane, 1083

subcellular protein localization, 1083Rhizobium meliloti

efn gene nodulation efficiency, 881exopolysaccharide synthesis loci, 66galactose metabolismDe Ley-Doudoroff pathway, 1092

P-D(1-2) glucan synthesisinternal-membrane enzymes, 947235-kilodalton protein intermediate,

947nod genes, 881

early essential functions, 881nodulation efficiency, 881

nodule formation, 66root hair curling, 66symbiotic megaplasmid, 66thiamine synthesis loci, 66

Rhizobium phaseolinifH and mob sequence rearrangements

heat treatment effects, 487nodulation modification, 487

symbiotic plasmidgenetic rearrangements, 487

Rhodobacter capsulatuscellulase gene expression

effect of host culture aeration, 604endoglucanase synthesis, 604exoglucanase synthesis, 604plasmid expression vectors, 604

Rhodopseudomonas capsulatachromatophores

cytoplasmic surface, 96exposed N-terminal region, 96light-harvesting polypeptides, 96

Rhodopseudomonas sphaeroidesintracytoplasmic membranes, 153photosynthetic membrane developmentgrowth initiation sites, role, 153synchronously dividing cells, 153

Rhodospirillum rubrumchromatophore membrane formationcytoplasmic-membrane invagination,

89immunocytochemical ultrastructural

analysis, 89Ribosomal gene transcription

E. colinutritional shift-up, 1095S10 transcription attenuation mecha-

nism, 1095Ribosomal protein phosphorylation

S. cerevisiaecyclic-AMP-dependent protein kinase,

713Rickettsia prowazekii

glucose acquisitionuridine 5'-diphosphoglucose trans-

port, 805RNA and protein synthesis

S. faeciumautoradiographic studies, 960cell mass-cell volume relationship,

960Rolling-circle DNA

E. colisynthesis initiation, 327

R100 plasmidoriT transfer origin gene

transcription terminators, 336traM and finP genes

cloning, mapping, and sequencing,336

Saccharomyces cerevisiaecholesterol uptake, 981ribosomal protein S10cyclic-AMP-dependent protein kinase,

713phosphorylation in vivo, 713

sterol uptakeantimicrotubule agents, 981cellular free cholesterol, 981steady-state cellular concentration,

981

VOL. 167, 1986

x SUBJECT INDEX

Salmonella typhimuriu'mcrp gene,

E. coli crp sequence homology, 616crp mutants

catabolite-repressible sugar fermenta-tion, 616

receptor protein-cyclic AMP interac-tions, 616

cyclic-AMP receptor protein genes, 616DNA adenine methylation mutantcysG locus, 420E. coli dam mutants, 420frameshift mutation induction, 420

glpQ productE. coli protein transport jamming, 462

mutant adenylate cyclase regulationgenes, 616NAD metabolism, 1086nadA cloning, 1086pnuC cloning, 1086

outer membraneslipopolysaccharide 0-antigen, effects,

1081Serospecific antigens

L. pneumophila, 893Shigella flexneri

crp gene comparisonE. coli crp, 639

Shigella sonneiphage T7 infectionDNA damage repair, 251phage T3 DNA ligase, 251unassembled capsid protein expres-

sion, 251Siderophore

V. anguillarum anguibactin, 57N. crassa molecular recognition, 1020

Small, acid-soluble spore proteinsB. cereus, 168B. stearothermophilus, 168B. subtilis spores

UV-light resistance, 174"T. thalpophilus," 168

Sorbitol productionZ. mobilis glucose-fructose oxidoreduc-

tase, 863SOS induction

E. coli ATP hydrolysis, 1055Spiroplasma spp.

disaturated phosphatidylcholinesincorporation and modification, 299

SporulationB. cereus respiratory systems, 544B. megaterium

gluconate formation, 149glucose catabolism pathway, 149

B. subtilisheat resistance reduction, 257intracellular serine protease gene, 110small, acid-soluble spore proteins, 174UV-light resistance, 174

Staphylococcus aureuscollagen binding

bacterial tissue colonization, 77cell surface receptor protein, 77fibrinogen effect, 77structural requirements, 77

exfoliative toxin B gene sequence, 726penicillin-binding-protein gene

P-lactam antibiotic resistance, 975molecular cloning, 975tobramycin resistance, 975

S. epidermidis plasmid homology, 888

transport-required N-terminal peptide,726

Staphylococcus epidermidisconstitutive macrolide-lincosamide-strepto-gramin B resistance plasmid pNE131nucleotide sequence, 888S. aureus plasmid homology, 888

Staphylokinase exportE. coli sak gene productpathway entrance requirement, 850precursor accumulation during syn-

thesis, 850Sterol uptake

S. cerevisiae, 981Streptococcus cremoris

lactose-metabolic genes, characteriza-tionexpression by chromosomal integra-

tion, 855Lac DNA transformation, 855Streptococcus spp. Lac' DNA ho-

mology, 855Streptococcus faecalis

bifunctional 6'-aminoglycoside acetyl-transferase 2"-aminoglycoside phos-photransferase geneantibiotic resistance, 631cloned gene regions, 631DNA sequence analysis, 631

Streptococcus faeciumcell mass, antibiotic effects

ampicillin, 562cephalothin, 562

cell mass-cell volume relationship, 960envelope growth sites, initiation

shape effects, 5621-lactam antibiotic effects

cell mass, 562envelope growth sites, 562

RNA and protein synthesisautoradiographic studies, 960cell volume effects, 960

Streptococcus lactisintracellular amino acid pool

major constituents, 522new amino acid, 522

N5-(1-carboxyethyl)-ornithinepurification and characterization, 522structure and synthesis, 522

Streptococcus sp.spg gene

expression in E. coli, 870immunoglobulin G-binding protein,

870Lancefield group G, 870nucleotide sequence, 870

Streptomyces glaucescensanthracycline antibiotic production, 575,

581tetracenomycin C

biosynthetic pathway, 575nonproducing mutants, 575, 581

Succinate dehydrogenaseM. phlei membranes

purification, 1solubilization, 1

Surface layer proteinA. serpens

diverse morphology, 1025three-dimensional structure, 1025

Surface-layer protein geneD. radiodurans

cloning in E. coli, 1048

HPI polypeptide expression, 1048Synechococcus heterospecific transforma-

tion, 1074Synechocystis sp.

cytoplasmic membranecarotenoid-binding protein, 396

Syntrophomonas wolfeifatty acid metabolism

cell-free extracts, 179enzymes, 179

phosphorylation reaction enzymes, 179

Tetracenomycin CS. glaucescens

biosynthetic pathway, 575nonproducing mutants, 575, 581

"Thermoactinomyces thalpophilus"small, acid-soluble spore protein genes,

168Tn9O7 transposition

B. megaterium, 716E. coli, 711

Toluene oxidationTOL plasmid pWWO, 455

Transposases, 429Trp repressor protein

E. coliintracellular levels, 272trpR autoregulation, 272L-tryptophan effects, 272

Tryptophanase operon expressionE. coli tna gene

leader peptide synthesis, role, 383Tyrosine

E. coli tyrP gene expression, 556

UltrastructureC. thermocellum cell surface cellulo-some, 828

Unassembled capsid proteinS. sonnei expressionphage T3 DNA ligase, effects, 251phage T7 infection, effects, 251

UV irradiation resistanceV. cholerae recA gene, 375

UV-light effectsE. coli ATP hydrolysis, 1055

Vibrio anguillarumanguibactin characterization, 57novel siderophore, 57

Vibrio choleraerecA gene

Hfr mating, 375homology with E. coli recA, 375methyl methanesulfonate resistance,

375UV irradiation resistance, 375

Vibrio fischeriluciferase expression

filamentous cyanobacteria, 411Vibrio harveyi

luciferase expressionfilamentous cyanobacteria, 411

Vibrio parahaemolyticuslateral flagella gene

laf transcription regulation, 210surface-dependent expression, 210

luminescence, 210swarming phenotype, 210

Vitamin B12

J. BACTERIOL.

SUBJECT INDEX xi

B. megaterium genes, 623Vitamin B12 transport

E. coliBtuCED polypeptides, role, 920cytoplasmic-membrane transport

genes, 920, 928

outer-membrane-transport genes, 920

Xanthone biodegradationArthrobacter spp.

catabolic intermediates, 8183,4-dihydroxyxanthone ring cleavage,

818

Zyinornonas inobilisglucose-fructose oxidoreductase

characterization, 863low substrate affinity, 863optimal pH values, 863sorbitol production, 863

VOL. 167, 1986