Johnny Yin 18 April, 2014Expert of yeast origin nutrients for Microorganisms

Contents123Introduction about Angel yeast Co.,Ltd.Angel yeast extracts Good nutrient-sources for microoganismsApplication of Angel yeast extract 1

1. Introduction of Angel Yeast Co.,Ltd.1.1 About us >1.2 Global net work > 1.3 Our history >1.4 Main business >1.5 Angels value chain >1.6 Employees >1.7 Advantages >

1.1 About us Angel yeast, founded in 1986, isa listed high-tech yeast company in China.It is one of the largest global yeast and yeast extract manufacturers with12factories in China and Egypt up to now.

1.2 Global network12Factories4Yeast extract plants12Technical centers140Countries

1989200020031986Project started Public companyNo.1 in Asia First facilitiesFirst yeast extract facilitiesGlobal supplier Today20081.3 History

The fastest growing company in global yeast industry

Angel yeast is estimated to grow at a CAGR of 20% 2011-2012, while the global yeast markets CAGR is to be 8.6% (Foodnavigator.com, 2012-04-05).

CAGR 2011-2012

Chart1

0.2

0.086

CAGR 2011-2016

Sheet1

CAGR 2011-2016

Angel20%

Global9%

Main business BakeryYeast extract-savoryYeast extract-fermentationBeverage & ethanolHuman healthEnzymesAnimal nutrition

1.4 Main business

1.5 Angels Value Chain MicroorganismIndustrial fermentationProductBrewingFeedPharmEnvironmentEnergyAgricultureFoodChemicalLabWine yeastEnzymeYeast extractYeast cell wallFeed yeastEnzymeActive yeastYeast extractInactive yeastYeast extractAlcohol yeastYeast extractBio-fertilizerBakeryEnzymeSeasoningInactive yeastYeast extractEnzymeYeast extractYeast extractInactive yeastTechnical supportExperimentPackageTrading

Our global partners

1.6 Employees>1331 employees, 70% of which are professional peopleOver 100 researchers work together with universities.7 international experts employed as senior technical consultants.

Rosenberg (Netherlands)He Chongyue (Taiwan, China) Yao Juan Li Xiao Kuang Jinbao Li Pei Li ZhijunTilak (U.S.)Jan Hille ((Netherlands)

1.7 Advantages1.7.1 Strong production output >1.7.2 Professional production process>1.7.3 R&D strength >1.7.4 Quality, safety and consistency >1.7.5 Certification>1.7.6 Environment protection >1.7.7 Global professional service >

1.7.1 Strong production output >

Yichang Plant Yili Plant Chongzuo Plant Binzhou Plant Total output of Yeast is 120.000 tons, Yeast Extract is 46.000 tons.

1.7.2 Professional production process

1.7.3 R&D strength >4 sub-headquarters with technical centers

National yeast technology research and promotion center

Post-doctoral scientific research workstation

R & D team led by PhDs and experts

National recognized laboratory CNAS

Basic research and Application research laboratory

Own the first-class test device and pilot plant.

1.7.4 Quality, safety and consistency > EU standard design production line

Stable and consistent quality applies to all production plants

Distributed control system

Siemens PCS7 process control system

Whole-course CIP cleaning

SAP-ERP Management flat

1.7.5 Certifications >BRCSEDEXISO22000HALALKOSHER(OU)ISO14001ISO9001CNAS

1.7.6 Environment protection >First class environment protection facilities in each plantThe high-concentration organic waste water was made into bio-organic fertilizer.

1.7.7 Global professional service >We do not only provide the best quality products, we also provide technical solutions for all our serving industriesSet up global baking centersOver 200 application engineers

2. Angel Yeast Extracts Good nutrient-sources for microoganisms

2.1 Whats yeast extract >2.2 Why choose yeast extract for fermentation industry > 2.3 Range of Angels typical products >

2.1 Whats yeast extract? Yeast extract is a kind of :

100% animal free natural ingredient that is made from yeast.

High performance and stable sources of nutrients for microorganisms

Clean and safe raw materials for industrial fermentation and culture media

Process of yeast extract production

2.1.1 Molecular weight distribution

2.1.2 Profile of Amino acids

2.1.3 Profile of free Amino acids and Total amino acidsFree Amino acids / Total Amino acids 100

2.1.4 Trace elements

Name of elementsConcentrationmg/kgCa246Mg149Zn86Fe33Cr1.26Mn 0.08K9172Na2022As0.03Se0.002Pb 0.19

2.1.5 Vitamins

Name of vitaminsConcentrationmg/kgVitamin B12.7Vitamin B256.9Niacin 336Vitamin B617.6Pantothenic acid29Free D-Biotin500 g/100g

2.1.6 Nucleic acid and Nucleotides

2.1.7 Functionality of yeast extract Increase biomass Improve the accumulation of metabolites

2.1.8 Stability of yeast extractTwo key indicators: Total nitrogen and Amino nitrogen, For example: Stability of Yeast extract FM902

2.2 Why we choose yeast extract for fermentation industry? Clean and Safe - Clean and safe natural material for culture media and industrial fermentation - Animal free, no risks of illness - GMO free, Non allergen - Kosher and Halal certified.

Stable quality - Produced with strict quality control system. - Large industry scale production - No quality change in different seasons

High performance - Advanced production technology

Beside yeast extract, Angel also produce yeast peptone and inactive yeast for fermentation industry

2.3 Range of Angels typical products

Microfiltered yeast extractFM888PowderLab, BiopharmaceuticalsNutrients-enriched yeast extractFM803FM505PowderPowderProbiotics, Starter cultureYeast peptoneFP101LP101PowderPasteProbiotics, culture mediaStandard yeast extractFM808FM902LM800LD00PowderPowderPasteLiquidIndustrial fermentationAutolyzed & Inactive yeastFM801YP101PowderPowderAntibiotics, APIsEconomical yeast extractFM701LM815PowderPasteAgriculture and environment

Which are the most popular products in lab research?

Angel Microfiltered yeast extract FM888

Features and Advantages - High solubility- Easily assimilated by microorganisms and cells- Rich in peptides and growth factors- Low impurities

Application- Lab research Dehydrated culture media, Cell culture, Diagnostic media- High value-added industrial fermentation Insulins, Hormones, Hyaluronic acids

Angel yeast peptone FP101

Features and Advantages - Good replacement of Animal origin peptone- Could be combined with yeast extract- Rich in peptides

Application- Lab research Microorganisms culture, small scale fermentation- Industrial fermentation Probiotics, Enzymes, Hormones, Organic acids

Which are the most popular products in industrial fermentation?

Angel standard yeast extract FM808

Features and Advantages - Good solubility- High nitrogen level- Rich in peptides- Light color

Application- Lab research Dehydrated culture media- Industrial fermentation Insulins, Hormones, Hyaluronic acids, Probiotics

Angel standard yeast extract FM902

Features and Advantages - Total soluble in water- High nitrogen level- Cost effective- High consistency

Application- Industrial fermentationFor >60 types of fermentation-based products

Angel standard yeast extract FM902

Angel standard yeast extract FM902

Angel standard yeast extract FM902

Angel standard yeast extract FM902

FM902EnvironmentAgricultureIndustrial fermentationAmino acidEnzymeHormoneOrganic acidProbioticNucleotides / NucleosidesPoly saccharideBiological preservativesAntibioticMashroomRhizobiaBiological baitEffective MicroorganismsBio-fertilizerWaste water treatment

Environmental microorganisms

Angel standard yeast extract LM800 and LD00

Features and Advantages - Paste and Liquid form of yeast extract FM902- Rich in peptides- Cost effective- High consistency

Application- Industrial fermentationFor >60 types of fermentation-based products

How to judge the quality?

Better yeast extract perform better solubilityDependsGood solubility means readily assimilated nutrients, but it depends on specific needs. Better yeast extract perform lighter colorDependsSome fermentation need light colorBetter yeast extract perform higher amino nitrogenDependsHigher amino nitrogen product is suitable for seed culture and probiotics, not all the fermentationYeast extract should contain low level active microbesTrueHigh level active microbes cause contaminationLess biomass and productivity means poor quality of yeast extractDepends40% of this situation means unsuitable process, condition or culture media formulaBakers yeast extract is better than Brewers yeast extractTrueBrewers yeast extract is not stable which come from spent brewers yeastBakers yeast extract must get Kosher/Halal certificatesTrueClean and safeYeast extract contain rich proteinDependsIt depends on the customers specific needs

3. Application of Angel Yeast Extract3.1 Summary of Angel yeast extract application >3.2 Some experiments of Angel yeast extract >

3.1 Summary of Angel yeast extract applicationBiomass products industry - Starter cultures and probiotics for human food products - Cultivation of mashroom and macrofungi Recommendation: FM808, FM803, FM505, FP101Culture media industry and lab reagent - Bench scale research - Dehydrated culture media, Diagnostics, Cell cuture Recommendation: FM888, FM808Bioactive molecules and chemicals industry - Amino acids and peptides - Organic acids, Vitamins, Nucleotides, Enzymes, Alcohol - Vitamins, Antibiotics, Hormones, Biological preservatives - Other types of large scale fermentation Recommendation: FM808, FM902, LM800, LD00, YP101Agricultural and environmental industry - Sewage treatment, Bio-pesticide, Bio-plastic - Probiotics for agriculture and environment Recommendation: FM902, FM801, FM701, LM815, LP101

3.2 Some experiments of Angel yeast extract 3.2.1 Morphology of 4 types of typical microorganisms >3.2.2 Some experiments of Angel yeast extract >

3.2.1 Morphology of 4 types of typical microorganisms after using yeast extract and other type of organic nitrogenABAAABBB AAnother type of organic nitrogen BAngel yeast extractStreptomyces lincolnensisLactobacillus bulgaricus Bacillus subtilis Acetobacterium

3.2.2 Angel standard yeast extract FM808 for Lactobacillus thermophilus growthStrain: Lactobasillus thermophilus

Medium: Yeast Extract FM808 0.3%Glucose 2%, etc. pH: 6.5 Result: Yeast extract FM808 could improve the growth of Lactobacillus thermophilus wellAngel yeast extract FM808ControlAngel yeast extract FM808Control

3.2.3 Angel standard yeast extract FM902 for the growth of E.coli and Absidia coeruleaThe growth curve of E.coli, comparison of FM902 and Brewers yeast extract The conversion rate of HC(from RSA), Absidia coerulea, yeast extract FM902

3.2.4 Angel nutrients enriched yeast extract FM803 for probioticsStrain: Lactobacillus rhamnosusMedium: MRSStrain: Lactobacillus helveticusMedium: MRS Lactobacillus needs complicated nutritional condition for its growth and acid production. Yeast extract is rich in amino acids, small peptides and growth factors which Lactobacillus needs. Yeast extract FM803 is a kind of free amino acids enriched product and could improve the growth of Lactobacillus.Angel yeast extract FM803ControlAngel yeast extract FM803Control

Fig.1 The effect on L-Tryptophan when different ratio of yeast extracts were used

Fermentation culture medium(%): yeast extract FM902, glucose 2 %, ammonium sulfate 0.4%, potassium dihydrogen phosphate 0.15%, etc.

Conclusion:FM902 is rich in small peptides and free amino acids, and it also contains appropriate polypeptides, vitamins and trace elements. Thus FM902 is suitable for the fermentation of L-Tryptophan, 2% is a proper level of yeast extract ratio.

3.2.5 Angel standard yeast extract FM902For L-Tryptophan

Department of Microbial Nutrition Research The research and development of new products The optimization of culture media formula The suggestion of fermentation process Development of tailor-made products

Technical support

Test and scale up platform

Any question, please feel free to contact me:

Johnny YinAngel Yeast Co.,Ltd.Address: 168 Cheng Dong Avenue, Yichang, Hubei, China. 443003.Email: yinqiang@angelyeast.com; johnnyyinq@gmail.com Contact information

THANK YOU!

Thank you very much for taking your time to be here in your so valued rest day. Im Johnny from Angel Yeast, my responsibility in our company is international business of yeast extract products which can be widely used for industrial fermentation and microbial culture media. Today, I would like to share some background knowledge about Angel and yeast extract. Hope my presentation could be helpful for you to understand Angel and yeast extract well.**This picture shows the global network of angel, you could find angel has set up 12 factories in the world include 4 yeast extract plants, 12 technical centers are located in China, southeast Asia and Africa, customers from more than 140 countries could buy angels products from local distributors. So you could find angels business covered most of the countries in the world. But, how long time does angel expand its business, what about angels history? So, next , let me draw your attention to the history of angel yeastIn this time line, you could find angel started in 1986, just like I mentioned before, and now we become one of the largest supplier and manufacturer of yeast and yeast extract, it goes through 28 years. Compared with some manufacturers from Europe with more than 100 years history, angel is really a new player in global yeast industry, but the growth is very fast.About how fast angel yeast grow during those years, I made a summary just like the left picture showed in this page, in 2011-2012, CAGR of Angel yeast, that means Compound Average Growth Rate, is 20%, but this data of global market is only 8.6%, so you could find how fast angel grow. As a supplementary, the right picture shows the yearly sales from 2000 to 2013, you could find angel keep fast and stable growth, and I believe this trend will continue in future.So, what kind of business does angel focus on? Briefly speaking, angels business cover 7 fields, that is bakery, yeast extract for savory and for fermentation, beverage and ethanol, human health, enzymes and animal nutrition. In those business, Bakery yeast is our core business, yeast extract is our fastest growing business. Angel developed those business just aim for serving the whole industrial chainI mean fermentation industry. Let me draw your attention to this picture, angels value chain, that shows how angels products could serve the whole fermentation industry. As you know, fermentation industry chain start from lab research, many useful strains and projects are developed from laboratory, and then, scale up and industrial scale fermentation, then get many useful fermentation-based products, those products could be used in brewing, food, agricuture, environment, chemical feed, energy and pharm industry. That is the whole industrial chain of fermentation industry. Angels target is supplying more and more products and service for the whole fermentation industry.Now our valued partners include many global famous company such as , so why angel could get customers trust about made in China?Because angel is a professional global yeast company, 70% of employees are professional people such as engineers, masters and PhDs, more than 100 researchers in University and instritute collaborated with angel and 7 international experts from EU, US, Taiwan and China mainland serve angel because of their rich experiences in yeast productionBesides professional employees, angel also has some advantagesFirst, strong production output, up to now, angel has 120,000tons yeast capacity and 46,000tons yeast extract capacity, that could meet the growing demand of yeast and yeast extract in the world. This is the basic process of yeast production, you could find we use our own selected yeast strains and culture it from bevel to large scale fermentor, then we could get active yeast or inactive yeast, yeast extract come from yeast cream which is a kind of cream after yeast fermentation, I will introduce the process of yeast extract in next part.Angel has strong R&D capabilityAngel produce its products with stable quality, safety and consistency, Angel get so many certificates such as Angel also pay much more attention to environment protection, because yeast production will bring some waster materials and waste water, we keep devoting ourselves to avoid pollution. Just like those picture showed in this page, angels production line is not harmful for the nature and some by products such as bio-organic fertilizer could be produced after waste water treatment.Angel also offer global professional servicesOk, well, this is an introduction about angel yeast, hope you have established a profile about angelLets go into second part, angel yeast extract, good nutrient sources for microorganisms.*First step, lets see whats yeast extract? ActuallyThis is the process of yeast extract production, This chart shows a typical molecular weight distribution of yeast extract, This chart shows a typical animo acids profile of yeast extract, This chart shows 3 typical free amino acids/total amino acids profile of yeast extractThis list shows typical data of elementsThis list shows typical data of vitaminsThis chart shows content of RNA and 5 types of nucleotidesSo, whats the main functionality of yeast extract? Actually, fist, yeast extract could be helpful for biomass increasing, because yeast extract perform better solubility in water and contain some nutrients which can be easily assimilated by microorganisms. Second, yeast extract could improve the accumulation of metabolites because the nutrition composition of yeast extract is complete, such as amino acids, nucleotides, vitamins, peptides, etc.Yeast extract is also a kind of stability nutrients for microorganisms, it could be produced with industrial scale and controlled by strict quality system, compared with other raw materials for fermentation(most of them are by-products from agriculture or animal), yeast extract is not affected by different seasons or places.*