Jessica Turner, USAPH Region North, LT Kirk Mundal USN, LCDR Craig Stoops USN,

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USAPHR-NORTH VECTOR-BORNE DISEASES DETECTION PROGRAM 2007-2010: WHAT WE DID FOR CONTINGENCY DIAGNOSTICS. Jessica Turner, USAPH Region North, LT Kirk Mundal USN, LCDR Craig Stoops USN, Melissa Miller USAPH Region-North,

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Jessica Turner, USAPH Region North, LT Kirk Mundal USN, LCDR Craig Stoops USN, Melissa Miller USAPH Region-North, . USAPHR-North Vector-Borne Diseases Detection Program 2007-2010: What we did for contingency diagnostics. . VECTOR-BORNE DISEASE SURVEILLANCE KIT. PHR-N. PHR-N. - PowerPoint PPT Presentation

Transcript of Jessica Turner, USAPH Region North, LT Kirk Mundal USN, LCDR Craig Stoops USN,

Page 1: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

USAPHR-NORTH VECTOR-BORNE DISEASES DETECTION PROGRAM 2007-2010: WHAT WE DID

FOR CONTINGENCY DIAGNOSTICS.

Jessica Turner, USAPH Region North, LT Kirk Mundal USN, LCDR Craig Stoops USN,

Melissa Miller USAPH Region-North,

Page 2: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

VECTOR-BORNE DISEASE SURVEILLANCE KIT

Kit based presentationDetailed instructionsCollection materialsPre-printed return envelope

PHR-NPHR-N

PHR-N

PHR-N

Page 3: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

UPON RECEIPT BY USAPHR-North Blood prepared and tested for appropriate

organismbased on animal species

○ PCR Ticks, fleas, etc.

IdentifiedPrepared and tested for appropriate organism

○ PCR Test results provided to submitter and other

appropriate recipients.

Page 4: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

ANIMALS SAMPLED 2007-2009

Bagram, AF Kandahar, AF Ramadi, IQ Bagdad, IQ

Dogs 38 24 6 1

Cats 62 191 7 2

Foxes 0 1 10 1

Jackals 5 25 6 1

Coyotes 13 0 0 0

Jungle Cats 0 1 1 3

Hedgehogs 0 2 0 1

Mouse 0 1 0 0

Cow 1 0 0 0

Sheep 1 0 0 0

Goats 3 0 0 0

TOTAL 123 244 30 9

Page 5: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

Ectoparasites Identified 2007-2009

Kandahar, AF Bagram, AF Ramadi, IQ Bagdad, IQ

Xenopsylla astia 3 6 0 0Ctenocephalides canis

6 9 0 9

Ctenocephalides felis

3 0 0 0

Pulex irritans 5 25 7 0Synosternus pallidus

0 0 3 0

Rhipicephalus turanicus

37 32 144 30

R. sanguineus 0 0 40 0Hyalomma anatolicum

0 27 0 0

Ixodes rubicundus 0 0 6 0

Page 6: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

Analysis PerformedPathogen Test Type

Anaplasma Real-time PCR

Babesia Conventional PCR

Borrelia Real-time PCR

Ehrlichia Real-time PCR

Rickettsia Real-time PCR

Page 7: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

Results of AnalysisKandahar, AF Bagram, AF

Babesia# POSITIVE/# tested % POSITIVE

CAT

R. turanicus 3 /19 16% 3 /6 50%

Whole Blood 13 /187 7% 3 /60 5%

JACKAL

R. turanicus 1/12 8% 0/4

Whole Blood 3/25 12% 0/5

DOG

R. turanicus none 1/14 7%

Whole Blood 2/24 8% 0/16

Page 8: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

Results of Analysis cont.Kandahar, AF Bagram, AF

Babesia# POSITIVE/# tested %POSITIVE

HEDGEHOG

R. turanicus 2/10 20% none

C. canis 1/1 100% none

Whole Blood none none

Page 9: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

Results of Analysis cont.Ramadi, IQ Baghdad, IQ

Babesia# POSITIVE/# tested % POSITIVE

CAT

R. turanicus 1/26 4% 0/3

JACKAL

R. turanicus 1/12 8% 2/8 25%

Page 10: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

Kandahar, AF 2008

Page 11: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

CONCLUSIONS

Babesia species circulating in the animal and ectoparasite populations

Vector-borne diseases information can be gained with this type of surveillance

Page 12: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

FUTURE Conclude analysis of 2009 Iraq samples Receive samples from Iraq and

Afghanistan in 2010Several cooperators have requested supplies

Refine capability to employ serological analysis on blood samples

Update analysis platform to allow concise results for multiple pathogens in one test run

Page 13: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

FUTURE cont. Apply epidemiological information to the

data Receive samples from livestock

Can analyze for other potential pathogens of importance

Page 14: Jessica Turner, USAPH Region North,  LT Kirk Mundal USN, LCDR Craig Stoops USN,

THANK YOU

By Cpl. Matthew K. Hacker from USA War Dogs