ISSN 0975-2722

Vol. XXX, No. 1-2 Hkkx&30] vad 1&2

January-June, 2009 tuojh&twu] 2009

Journal of Drug Research in Ayurveda and Siddha

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Published by

CENTRAL COUNCIL FOR RESEARCH IN AYURVEDA AND SIDDHA

NEW DELHI

Editorial and Business Office:

Jawahar Lal Nehru Bhartiya Chikitsa Evam

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Institutional Area, No. 61-65

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Annual Subscription In India Rs. 120/-; Abroad US $ 40

Journal of Drug Research in Ayurveda and Siddha

Vol. XXX, No.1-2

January-June, 2009

PATRON

Union Minister for Health & Family Welfare

(Government of India)

Editor-in-Chief

Prof. G.S. Lavekar

Editor

Dr. M.M. Padhi

Editorial Board

Dr. S.N. Gaidhani

Dr. G.V.R. Joseph

Dr. B.P. Dhar

Dr. Prameela Pant

Dr. Neera Vyas

Dr. M.M. Sharma

JOURNAL OF DRUG RESEARCH IN AYURVEDA AND SIDDHA

EDITORIAL ADVISORY BOARD

Dr. Lohar Dr. (Mrs.) Veena Gupta

Dr. Raman Singh Dr. S.H. Parihar

Dr. Shamshad Ahmed Dr. B.M. Singh

Dr. Y.K.S. Rathore Dr. S. Rajasekharan

Dr. K.S. Nagaraja Prof. P. Das

Dr. S. Srivastava Dr. Manisha Sarkar

Dr. Bikram Singh Dr. V.K. Lal

Dr. Naveen Khare Dr. (Mrs.) Shanta Mehrotra

Dr. Suresh Walia Dr. Rajeev Sharma

Prof. V.K. Kapoor Dr. Roma Mitra

Dr. H.B. Singh Dr. S.D. Kamat

Dr. V.K. Singh Dr. S.J.S. Flora

Dr. K.C. Bhatt Dr. Y.P. Sahni

Dr. K.S. Negi Dr. K.K. Srivastava

Editorial Support

Mr. N.K. Gupta Mr. Upendra Singh Mr. Syed Fahar Ali

JOURNAL OF DRUG RESEARCH Vol. XXX, No.1-2

IN AYURVEDA AND SIDDHA January-June, 2009

CONTENTS

S.No. Particular

Page No.

1. Evaluation of in vivo anthelmintic efficacy of Swertia chirata (Chirayata) against Gastrointestinal Nematodiasis in Goats

Manpreet Arora, Y.P. Sahni, Vidhi Gautam and Alok Singh

1-8

2. Ethno-Medicinal studies on plants used in the treatment of Urino-genital Disease by Bhil Tribe of Ratlam District (M.P.)

Dinesh Jadhav

9-18

3. Hepatoprotective Activity of Withania somnifera Dunal (Ashwagandha) on Paracetamol induced liver damage in

Albino Rats

Y.P. Sahani and Vidhi Gautam

19-26

4. Pharmacognostical study of roots of Merremia emarginata (Burm. F.) Hall. F. and Merremia aegyptia (Linn.) Urban

Suman Kumari, V.J. Shukla and Alka Agarwal

27-38

5. Effect of Decoction of Butea monosperma (Linn.) Kuntze and Daucus carota (Linn.) Var. sativa seeds on oestrous cycles

of Female Albino Rats

Shilpa Donga, Vinay J. Shukla, Sarada Ota, B. Ravishankar

And I.U. Mistry

39-48

6. Development of standard operating Procedure and Pharmacopoeial standards for Raca Kanti Meluka (RKM) A Siddha Formulation

A. Saraswathy, Arunmozhi Devi and Nandhini Devi

49-58

EVALUATION OF IN VIVO ANTHELMINTIC

EFFICACY OF SWERTIA CHIRATA

(CHIRAYATA AGAINST GASTROINTESTINAL

NEMATODIASIS IN GOATS

Manpreet Arora 1, Y.P. Sahni 2, Vidhi Gautam 3 and Alok Singh 4

(Received on 14.12.2008)

Investigations were conducted to evaluate in vivo anthelmintic efficacy of aqueous extract of

seeds of Swertia chirata (Chirayata) against some of the most common pathogenic gastrointestinal

nematodes such as Haemonchus, Bunostomum and Oesophagostomum spp. in naturally infected

goats. The aqueous extract was administered at the dose of 550 mg/kg body weight, orally for 10

consecutive days in adult Goats naturally infected with gastrointestinal nematodes. The

anthelmintic activity of S. chirata was compared with Albendazole (5 mg/kg, oral), a standard

anthelmintic drug used in clinical practice. The anthelmintic efficacy of aqueous extract was

assessed by determining the mean EPG count on day 0, day 3, day 6, day 9 and day 12

respectively. Examination of the fecal samples revealed significant and progressive reduction in

the number of eggs in feces (EPG) from second post treatment day onwards and till day 12. The

anthelmintic efficacy based on fecal scores in Goats treated with S. chirata and Albendazole on

day 12 of the experiment was found to be 62.50% and 100%. The findings indicated that S. chirata

exhibited significant in vivo anthelmintic efficacy against gastrointestinal nematodes in Goats.

1 & 4

M.V. Sc. Student 2 Associate Professor

3 Asst. Professor Dept. of Pharmacology & Toxicology, College of Veterinary Science & Animal Husbandry to

Jabalpur

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fpjk;rk ds chtk ds tylRo dk ijh{k.k cdfj;k e lk/kkj.kr% ik;s tkus okys vkrd`fe tSls&gheksUdl] C;wuksLVksee rFkk blksQxksLVksee ds fojks/k e ijthoh ukkd lf;rk dks tkapus ds fy, izkd`frd :i ls izHkkfor cdfj;k e fd;k x;k gSA fpjk;rk ds tylRo dks 550 fe-xke@fdyksxzke@tkuoj eq[k ls nl fnu rd yxkrkj fn;k x;kA tylRo dh ijthoh ukkd {kerk Mean EPG ds }kjk thjks fnu] rhljs fnu] ukSo fnu o ckjgo fnu ij fu/kkZfjr dh xbZA cdfj;k ds esy ds ijh{k.k }kjk ;g fl) gqvk fd fpj;rk ds tylRo dks nsus ds nwljs fnu ls gh ijthoh ds vaMk dh la[;k e deh vkuh 'kq: gks xbZA fpj;rk ds tylRo dh ijthoh ukkd {kerk dk ey ijh{k.k Ldksj cdfj;k e ckjgo fnu ds fpfdRlk mijkar 62-50 izfrkr jgk tcfd vWycsUMktkWy dh 5 fe-xke@fdyksxzke@tkuoj ijthoh ukkd {kerk dk ey ijh{k.k Ldksj 100 izfrkr jgkA

ETHNO-MEDICINAL STUDIES ON PLANTS USED

IN THE TREATMENT OF URINO-GENITAL

DISEASE BY BHIL TRIBE OF

RATLAM DISTRICT (M.P.)

Dinesh Jadhav1

(Received on 17.12.2008)

Frequent ethnomedicinal surveys were conducted during August 2004 to March 2007 in

different tribal inhabited villages of Ratlam District (M.P.). Result of these surveys indicated that 34

plant species are interestingly used for the treatment of urinogenital diseases prevalent in the Bhil

tribe of the district. Botanical names followed by family, local names and the ethnomedicinal uses

have been provided.

1 30, Rajiv (Kasturba) Nagar, Ratlam 457001 (M.P.) INDIA

lkjkak

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jksxk ds mipkj e yk;s tkus okys iknik dk yksdoukS"k/kh; v/;;u

fnusk tk/ko

jryke tuin ds fofHkUu xkok dk vxLr 2004 ls ekpZ 2007 ds e/; ,d yksd oukS"k/kh; losZ{k.k fd;k x;kA blds ifj.kkeLo:i Kkr gqvk fd bl tyin e fuokl djus okyh Hkhy tutkfr 34 ikni iztkfr;k dks viuh ew= ,oa tuusfUnz; laca/kh fofHkUu O;kf/k;k dh fpfdRlk e jkspd rjhds ls iz;ksx djrh gSA bl izi= e bu 34 ikni iztkfr;k ds okuLifrd uke ds lkFk gh dqy ds uke] LFkkuh; uke o yksdks"k/kh; mi;ksx fn;s x;s gSA

HEPATOPROTECTIVE ACTIVITY OF WITHANIA

SOMNIFERA DUNAL (ASHWAGANDHA) ON

PARACETAMOL INDUCED LIVER

DAMAGE IN ALBINO RATS

Y.P. Sahani 1 and Vidhi Gautam2

(Received on 24.12.2008)

In the present study, aqueous and alcoholic extracts of Withania somnifera were screened for

their hepatoprotective activity on paracetamol induced liver damage in albino rats. The percent

hepatoprotective activity of Withania somnifera, in terms of three major enzymes of liver viz.

SGOT, SGPT and ALP was found to be 91.30%, 77.77% and 65.4% for alcoholic extract and

74.08%, 68.92% and 53.75% for aqueous extract, respectively. The study showed that alcoholic

extract of Withania somnifera was more effective than alcoholic extract but its efficacy was found

to be lesser than standard hepatoprotective drug LIV-52.

1 Professor, College of Veterinary Science & Animal Husbandry, Jabalpur (M.P.) 482001

2 Asstt. Professor, College of Veterinary Science & Animal Husbandry, Jabalpur (M.P.) 482001

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ok;- ih- lkguh ,oa fof/k xkSre

izLrqr v/;;u e] voxa/kk ds tylRo o ,sYdksgy lRo dk iSjklsVkekWy }kjk ;d`r ukk gksus ij ;d`r cpko dk ijh{k.k pwgk ij fd;k x;k gSA voxa/kk dh ;d`r cpko lf;rk dk v/;;u] ;d`r ds ,atkbEl ds izfrkr }kjk fd;k x;k gSA ;d`r ds rhu cM+s ,atkbEl ,l-th-vks-Vh-] ,l-th-ih-Vh- rFkk ,-,y-ih- dk ;d`r cpko izfrkr ,sYdksgy lRo e 91.30%, 77.77% rFkk 65.46% ,oa tylRo e 74.08%, 68.92% rFkk 53.75% ik;k x;kA bu ijh{k.kk ls ;g izdV gqvk fd voxa/kk dk ,sYdksgy lRo] blds tylRo ls vf/kd ;d`r cpko e izHkkodkjh gSA

PHARMACOGNOSTICAL STUDY OF ROOTS OF

MERREMIA EMARGINATA (BURM. F.) HALL. F.

AND MERREMIA AEGYPTIA (LINN.) URBAN

Suman Kumari 1, V.J. Shukla 2 and Alka Agarwal 3

(Received on 13.01.2009)

Merremia is one of the important genus of family Convolvulaceae and this family is known

for the presence of glycosidal resin and its purgative property. Merremia aegyptia (Linn.) Urban

and Merremia emarginata (Burm. F.) Hall. F. are the two plants of the same genus belonging to

this family. The microscopic characters of Merremia aegyptia roots showed thick walled cork

cells, small clusters of rosette calcium oxalate crystals and slightly larger xylem vessels than

Merremia emarginata. The characteristic features of Merremia emarginata roots were one of two

layers of suberized cork cells and uni or bi seriate medullary rays. The cells of cortical region were

slightly polygonal to wavy in shape in Merremia aegyptia, while in Merremia emarginata

parenchymatous cells of cortex region were polygonal to spherical in shape.

TLF profile of Merremia aegyptia showed more intense and significant spots in selected

solvent system. Both the plant extracts showed many common spots having similar R1 and colour

indicating more or less similar constituents but with different composition.

1 Senior Research Fellow (Chemistry), CCRAS, Janakpuri, New Delhi - 110058

2 Research Officer (Chemistry), CCRAS, Janakpuri, New Delhi - 110058

3 Co-opted Lecturer (Phytochemistry), IAMPS, Gujarat Ayurved University, Jamnagar, Gujarat

lkjkak

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vcZu ds ewy dk Hks"kt vfHkKkuh; v/;;u

lqeudqekjh] oh-ts- 'kqDyk ,oa vydk vxzoky

esfjfe;k dkUokYoqyslh ifjokj dk ,d egRoiw.kZ oak gSA vkSj ;g ifjokj XyksbdksflMy jsftax ,oa 'kks/ku lEifk ds fy, tkuk tkrk gSA esfjfe;k bftfIVvk fyUu vcZu ,oa esfjfe;k bejftukVk ceZ ,Q- gky- ,Q-

blh ifjokj ds ,d oak ls laca/k j[krs gA lw{enkhZ v/;;u e esfjfe;k bftfIVvk ikni ds ewy esfjfe;k bejftukVk dh rqyuk l?ku nhokj ls f?kjh gqbZ dkdZ dksfkdkvk dSyfk;e vkWDlysV ffLVy ds y?kq lewg ,oa nk#rarq ik= i znfkZr djrs gSA ,d ;k nks lrg dh lqosjuhdj.k dkdZ dksfkdk, vkSj ,dh; ,oa }hiafDr eTtk fdj.k esfjfe;k bejftukVk dh fok"V foks"krk gS esfjfevk bftfIVvk e izkarLFkk {ks= dh dksfkdk, dqN cgqeq[kh ls rjaxhr vkdkj dh gksrh gS] xksykdkj vkd`fr dh gksrh gSA

esfjfe;k bftfIVvk fyUu vcZu dh Vh ,ylh :i js[kk e vf/kd ,oa egRoiw.kZ fukku ?kksy }kjk ik;s x;sA nksuk iknik ds vdZ us dqN leku vkj-,Q- ,oa jax ds ladsr fn;s fd ,d gh la?kVd ;k vo;o dks iznfkZr djrk gSA

EFFECT OF DECOCTION OF BUTEA MONOSPERMA

(LINN.) KUNTZE AND DAUCUS CAROTA (LINN.)

VAR. SATIVA SEEDS ON OESTROUS CYCLES OF

FEMALE ALBINO RATS

Shilpa Donga 1, Vinay J. Shukla 2, Sarada Ota 3,

B. Ravishankar 4, And I.U. Mistry 5

(Received on 23.03.2009)

The anti-ovulatory activity of decoction of Butea monosperma (Linn.) Kuntze (Bacaceae)

seeds and Daucus carota (Linn.) Var. sativa (Umbelliferae) seeds were studied in rats. The

decoctions were administered to animals orally for 28 days. The vaginal smears were examined

daily for the number and duration of oestrous and di-oestrous cycles. The oestrous phase was

reduced while the di-oestrous phase was persistent and prolonged in both the treatment groups,

thus revealing anti-fertility potential of these herbs.

1 & 5

Lecturer, Deptt. of Streeroga & Prasutitantra

2 C-opted Lecturer

3 Research Officer (Ayurveda), CCRAS, New Delhi - 110058

4 Head in I.M.P.S., Deptt.of Pharmacology, I.P.G.T. & R.A., Gujarat Ayurved University, Jamnagar, Gujarat

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,l- ch- nkxk] oh- ts- 'kqDy] ,l- vksVk] ch- jfo 'kadj ,oa vkbZ- ;q- feL=h

izLrqr 'kks/ki= e iykk cht DokFk ,oa xktj cht DokFk dh xHkZ fujks/kd ,UVh&vkO;qysVjh vlj ns[kus ds fy;s eknk pwgk ds bLVl p dk v/;;u fd;k x;k gSA iykkcht DokFk 1620 fexzk@fdyksxzke 'kjhj Hkkj rFkk xktj cht DokFk 2160 fexzk@fdyksxzke 'kjhj Hkkj ls 28 fnu rd nsus ij bLVl fnuk e egRoiw.kZ deh ikbZ xbZ] tcfd gkbZ&bLVl e o`f) ,oa LFkk;hRo ik;k x;kA bl izdkj vkO;qysku dks jksd dj xHkZfujks/kd :i e mi;ksxh gks ldrk gSA

DEVELOPMENT OF STANDARD OPERATING

PROCEDURE AND PHARMACOPOEIAL STANDARDS

FOR RACA KANTI MELUKA (RKM) A SIDDHA FORMULATION

A. Saraswathy 1, Arunmozhi Devi 2 and Nandhini Devi 3

(Received on 29.09.2009)

Siddha medicinal system is an ancient system, practiced for over 5000 years. Siddha

experts indicate that Siddha formulations have been proved to be useful in controlling venereal

disease (VD) including diseases closely resembling to HIV clinically. The drugs like Raca Kanti

Meluka (RKM), Amukkara curanam (AC), Nellikkai ilakam (NI) and in combination of these

drugs were reported to be useful in HIV patients for both clinical improvement and elevation of

CD4 counts. In the present study, an attempt has been made to develop Standard Operating

Procedure for the preparation and Pharmacopoeial standards for Raca Kanti Meluku. The

developed SOP and analytical standards can be used for fixing standards in Siddha

Pharmacopoeia.

1 Assistant Director In-charge

2 & 3

Senior Research Fellows, Captain Srinivasa Murti Drug Research Institute for Ayurveda and Siddha (CCRAS),

Arumbakkam, Chennai - 600106

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,- ljLorh] v:.keks>h nsoh ,oa uUnuhnsoh

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