iWorx Cardiovascular

Research Solutions

ApplicationsBlood pressureECGBlood flow and cardiac outputPressure-VolumeSonomicrometry dimension and volumeIsolated Langendorff and working heart modelsIsolated tissue studies

iWorx offers complete systems to studyphysiological function for a variety ofscientific research applications. Ourcardiovascular products are used toassess cardiac performance andhemodynamics both in vivo and in vitro.We offer transducers, accessories,amplifiers, data recorders and softwarefor in situ experiments, isolated heartpreparations and isolated tissueprotocols.

Data Acquisition and Analysis

for the Life Sciences

LabScribe2 Software

LabScribe2 software is a powerful recording and analysissoftware package offered by iWorx. LabScribe2 has anintuitive, user-friendly interface for setting up acquisitionscreens, calibrating signals and analyzing data. Up to 128channels of data can be displayed simultaneously at samplingrates as high as 100,000 samples/second.

A comprehensive set of analysis routines have beenpreconfigured making data analysis and interpretation quickand easy. Specific analysis modules for cardiovascularresearch are available to analyze pressure signals, blood flowdata, ECG recordings, ventricular pressure-volume loops andsonomicrometry dimension data.

LabScribe2 includes a scripting function for use if the built-inanalysis tools fall short of what is required for your application.Scripting languages currently supported include: Dadisp,Python, MatLab, LabView, and C++.

Features

DisplayLabScribe2 software displays data through a customizable,user-friendly, and intuitive interface. The number of input andcomputed channels, the display time, and scaling of the X andY axes are just a few of the parameters that can be saved to aviewing template. Simply click one button to change from oneview to another.

Real-time Online CalculationsMonitor and observe functional parameters during anexperiment with an array of real-time calculations and user-defined computed channels. Labscribe2 software cancalculate rates, min/max/mean values, systolic/diastolic valuesas well as derivatives and integrals in real-time. A uniquechannel math function allows users to create their ownformulae for viewing signals in real-time.

Data ReductionData can be analyzed and reduced in the Analysis window.Selections of data can be averaged and written to theLabScribe2 Journal or directly to a text file. For example, thesoftware can be instructed to break a file up into five-minutepieces, determine the average heart rate for the data in eachsegment, and write those values to a report.

LabScribe2 interface showing data being acquired in real-time.

Changing how data is displayed can easily be accomplished by

setting up different views.

Data can be saved for several hours in one file. Marks can be entered

to easily navigate to specific time points for data viewing and

reduction.

LabScribe2 Software

Complex mathematical calculations are available for analyzing any

cardiac signal. Examples include some of the pressure-volume loop

algorithms included with LabScribe2.

Real-Time AnnotationsMark data during an experiment with free-form text notes.These marks are searchable and appear in the field of data towhich they apply. Marks are time locked to the record and canbe used to “Go To” any marked region of the data. Marks canbe preset and assigned as keyboard shortcuts for quick andeasy annotation.

Advanced AnalysisSpecific time points and features of a signal can beAutoMarked for further analysis and validation. For example,the ECG AutoMark feature will place marks at the Q, R, S, T,and P waves. The blood pressure AutoMark will place marks atsystolic and diastolic points, dichrotic notch and max/min dP/dt.

XY WindowAny two channels can be displayed in XY format, online oroffline. LabScribe2 software supports area measurements andsegment slopes from such plots. And, of course, you can savethe plots as pictures or text.

Settings filesSettings files can be created for different experimental protocols. The number of input channels, calibration parameters, graph settings, computed channels, views andanalysis protocols are all saved to a settings file.

FFT analysisLabScribe2 includes a Fast Fourier Transform feature for frequency analysis of any signal.

Input/OutputLabScribe2 supports various pacing and stimulation protocolswith a built-in stimulator interface which allows delivery of a single, continuous, or train of pulses. Pulse durations, frequencies and amplitudes can be changed as the protocol isbeing delivered. The software also offers full control of digitalI/O lines that can be used to count events, or control devices inthe lab environment.

ExportAll recorded data can be exported to either a text file or a picture (.png, .jpg) making reports or posters much easier tocreate. Of course, you can always print your data from anywindow at any scale.

ImportAn optional import module is available so that data acquiredfrom other acquisition systems can be analyzed withLabScribe's powerful analysis routines.

Data from Automarks are sent to the journal from where they can be

exported easily to Excel or another analytical program for further

analysis.

FFT analysis can be performed on any signals. For example, heart

rate variability can be calculated using either ECG or Blood pressure

from this menu.

Proper assessment of cardiac function and hemodynamicperformance requires a measurement of pressure from withinthe heart and the peripheral arteries. iWorx offers severalsolutions for measuring pressure including fluid-filled pressuretransducers, catheter-tipped micro pressure sensors and non-invasive tail cuff pressure systems.

Systems and Components

Fluid-Filled Catheter TransducerThe BP-100 blood pressure transducer is an economical andaccurate device used to measure direct arterial or venouspressure in animals using the fluid-filled transducer technique.A precision laser-trimmed chip provides accurate and linearmeasurements over a broad range of pressures. Pressurechanges in the vessel are transmitted within the fluid-filled tubeto the pressure transducer and acquired for monitoring andfurther analysis. Tubing is available as small as 1 mm in outsidediameter making this technique well-suited for measuringpressures in very small vessels.

Catheter-Tipped TransducersiWorx offers catheter-tipped solid-state pressure catheters insizes ranging from 1.2F to 1.6F. These catheters arerecommended for measuring pressure in small vessels as wellas ventricular pressures in small rodents. Solid-statetransducer-tipped catheters offer superior sensitivity andtemporal resolution compared to fluid-filled catheters and aregenerally more suitable for measuring intra-cardiac pressuresfrom animal species with high intrinsic heart rates.

Non-invasive Blood PressureInvasive measures of pressure are not always possible and, asa result, there is a need for non-invasive methods to detectpressure. The most widely accepted technique for animals isthe tail-cuff method.

iWorx offers the IITC noninvasive blood pressure systems formice and rats. The IITC Life Science amplifier with built-ininflation outputs systolic pressure, mean pressure, diastolicpressure and heart rate as analog signals which can beacquired by iWorx data recorders. Systems include the amplifierwith built-in cuff pump, sensor and restrainer, cables,sphygmomanometer and accessories. Warming chambers areavailable so that no heating pads are required.

Blood Pressure Measurement

BP-100 and BP-100RE Intravascular Blood

Pressure Transducers measure direct

arterial or venous pressure in animals.

FTM-1211B-0018 1.2F Mouse Pressure Catheter

FTM-1611B-0018 1.6F Rat Pressure Catheter.

IITC-229M Noninvasive Blood Pressure System -

Single Mouse and IITC-229R Noninvasive Blood

Pressure System – Single Rat.

Arterial pressure calculations:

Heart Rate (HR)Maximum Pressure (Pmax) Minimum Pressure (Pmin)Mean Arterial Pressure (MAP)Systolic and diastolic pressureMaximum dP/dt (dP/dtmax) Minimum dP/dt (dP/dtmin)Pulse HeightNotch PressureSystolic DurationDiastolic DurationCycle Duration Time To PeakElection TimePercent Recovery

Ventricular pressure calculations:

Heart Rate (HR)Maximum Pressure (Pmax) Minimum Pressure (Pmin)Mean Pressure (Pmean)End-systolic Pressure (Pes) End-diastolic Pressure (Ped)Maximum dP/dt (dP/dtmax) Minimum dP/dt (dP/dtmin)Developed PressuredP/dt@Contractility IndexRelaxation IndexSystolic DurationDiastolic DurationCycle DurationTension Time IndexRelaxation TimeIsovolumetric TimeTau (Weiss, Logistic, Glantz, Mirsky)

Blood Pressure Analysis

Acquire and analyze data from blood pressure transducers. Data can be analyzed from ventricular

and arterial signals in real-time.

The blood pressure module automatically calculates common indices of function from the blood

pressure signal where it can easily be exported to another program for further analysis.

Electrocardiograms (ECG/EKG)

Record and AutoMark the ECG, sending the calculations to the journal from where they can be

exported easily to Excel or other analytical program for further analysis.ECG calculations:

Heart rateTime at RR-R IntervalDelta R-RPR IntervalP DurationQRS DurationQT IntervalQTc IntervalST SegmentT DurationT-P Duration

Heart Rate Variability (HRV*) calculations:

Delta R-RLow PowerHigh PowerMean Power at low bandMean Power at high band

*Note: HRV can be derived from either an ECG trace or a blood pressure trace.

Electrodes

C-ISO-255 3-Lead Isolated wide bandwidth cable for ETH-256C AmplifierC-ISO-104 3-Lead Isolated ECG Amplifier CableA-NE-300 Set of 3 Needle Electrodes with Safety Pin ConnectorsA-NE-500 Set of 5 Needle Electrodes

with Safety Pin ConnectorsC-WT-100 The Wilson Terminal Cable allows simultaneous recording of periodical leads and one chest lead when used with iWorx 214 RecordingInterface and a C-ISO-104 3-Lead Isolated ECG Amplifier Cable.

The cardiac cycle involves a sequentialcontraction of the atria and ventricleswhich is triggered by the coordinatedelectrical activity of the myocardial cells inthe heart. These electrical currents can berecorded non-invasively through electrodesproducing what is known as anelectrocardiogram or ECG. The ECG canbe measured in 3 dimensions by looking atthe electrical signals from different viewsor leads. iWorx offers complete systemsfor humans and animals to study ECG insome of the most common leadconfigurations - 3 lead ECG, 6 lead ECG,12 lead ECG.

Amplifier/Signal Conditioner Options:

ETH-256C Two Channel Combination Bridge/ECG/EMG/EEG AmplifierSAE-100 Small Animal ECG Amplifier SAE-200 Small Animal ECG Amplifier

Data Recording Options:

IX-300 seriesIX-400 series

Intracardiac Electrical Potentials

iWorx offers minimally invasive 8-electrodecatheters for studying intracardiacelectrical potentials in the rodent.Intracardiac electrophysiology cathetersallow for in depth analysis of biopotentialpropagation through the heart. Up to 4regional intracardiac electrograms can berecorded simultaneously. The samecatheter can be used to pace the heartwhile recording electrical activity.

Applications

His Bundle characterizationSA node characterizationGeneral signal propagationEndocardial ECG assessmentRegional atrial assessmentConduction studiesQualification of cardiac insult

Amplifier/Signal Conditioner Options:

ETH-256C Two Channel Combination Bridge/ECG/EMG/EEG AmplifierSAE-100 Small Animal ECG Amplifier SAE-200 Small Animal ECG Amplifier

Data Recording Options:

IX-300 seriesIX-400 series

Sensors/Electrodes:

A-NE-100 Subdermal Ground Electrode

System Components:

FTS-1113A-0518 1.2F Cardiac Electrogram CatheterFTS-1913A-1018 1.9F Cardiac Electrogram CatheterFI893B Catheter Interface Box (includes extension cable)

FI893B Catheter Interface Box with

FTS-113A-0518 Catheter.

Intracardiac electrical potentials look similar to a surface ECG. Multiple signals from different

locations can be recorded simultaneously and analyzed from a single catheter.

PV Loop Calculations:

Maximum and Minimum VolumeDiastolic and Systolic VolumeStroke VolumeCardiac OutputEjection FractionEnd-Systolic Pressure VolumeRelationship (ESPVR)End-Diastolic Pressure VolumeRelationship (EDPVR)Preload-Recruitable StrokeWork (PRSW)Pressure-Volume Area (PVA)Arterial ElastanceTime-Varying ElastanceMaximum PowerPreload-Adjusted MaximalPowerPotential EnergyCardiac EfficiencyHeart Rate Variability (HRV) Calculations

Plotting:

PV LoopsPRSWMax dp/dt vs EDVPVA vs EDVPVA vs ESP

Pressure Volume Loops

Pressure-volume loops have become thegold standard for studying myocardialcontractility, compliance, muscle energeticand other quantitative measures of cardiacfunction. iWorx offers a comprehensiveacquisition and analysis solution for PVloop experiments. Our LabScribe2 PVloop module can be used to analyze PVdata from any source includingconductance catheters, admittancecatheters or sonomicrometry dimensioncrystals.

Elastance and compliance curves can begenerated in real-time from within theacquisition interface providing instantfeedback on the quality of the occlusionsbeing performed. Unlike other softwareofferings for PV analysis, all the acquisitionand analysis takes place within oneprogram providing seamless and timesaving options for generating data forpublication.

Complete Systems iWorx offers the Scisense ADVantagesystem for rodents which yields the mostaccurate, reliable and repeatablemeasurements of pressure-volume data.Scisense catheters range in size from1.2F to 1.6 F and can acquire bothpressure and volume data from a singlecatheter. Typical applications includeheart failure studies, gene therapy andmanipulation protocols, and pharmacologystudies.

Benefits of the ADVantage system include:No Saline bolus requiredReal-time measurement of parallel conductanceAbsolute ventricular volumesLower standard deviationsAccurate, reliable, and reproducible data

Data Recording Options:

IX-300 seriesIX-400 series

Components

FV897B ADVantage PV SystemFTS-1212B-4518 1.2F mouse PV catheterFTS-1912B-8018 1.9F rat PV catheterFC-ADV-PK Cable packageFM-1287-1 mouse myocardial probeFM-1987-1 rat myocardial probeHI76301ADV Conductivity probeLS20/PVL software module

Typical screen configuration for acquiring PV data in the rodent. Signals being displayed are LVP,

LVV, Phase, Magnitude and dP/dt as well as an XY plot depicting LVP vs. LVV. A PV Loop

AutoMark feature can be used to display key delineators and write data to the online journal in real-

time.

Scisense FV897B Pressure-Volume System

Isolated Heart Preparations

Record and AutoMark LDEVP, systolic and diastolic from a blood pressure curve, sending the

calculations to the journal and a spreadsheet file in real-time.

The isolated perfused heart system, asoriginated by Oscar Langendorff more thana century ago, has become a mainstay ofpharmacological and physiologicalresearch. The system allows theexamination of cardiac inotropic (cardiaccontractile strength), chronotropic (heartrate), and vascular effects without thecomplications of an intact animal model.The original design has evolved toencompass both constant pressure andconstant flow models in recirculating ornon-recirculating modes, as well asworking heart models. iWorx providestransducers, accessories and dataacquisition hardware and software to beused in any of these configurations.

Modes

Langendorff:Constant pressure recirculatingConstant pressure nonrecirculatingConstant volume recirculatingConstant volume non-recirculating

Working Heart

PacingFor pacing the heart during a Langendorffpreparation, the built-in digital stimulator ofan iWorx data acquisition unit can be used.iWorx stimulators have very flexiblecontrols, which even allow real-timechanges in stimuli parameters to be made(with the touch of a button) throughLabScribe2 software.

Force, Pressures, Flow, and VolumesContractile forces, pressures, flow, andvolumes can be measured using anycombination of recording electrodes,strain-gage transducers and amplifierswhich are available from iWorx. K+, Ca2+,Cl- and pH can also be measured directlyfrom the perfusate by using electrodesconnected to an iWorx signal conditioner.O2 consumption can be measured byplacing an O2 electrode in the inflow andin the effluent and calculating thedifference in concentration.

ECGElectrocardiograms can also be recordedfrom the isolated heart using electrodesconnected to any of our low noisebiopotential amplifiers.

Transducers

BP-100 Blood Pressure TransducerFT-302 Force TransducerISE-100 pH ElectrodeDO2E-200 Oxygen Electrode/variousion electrodesTM-100 Temperature Electrode

Amplifier/Signal Conditioner Options:

ETH-256C Two Channel Combination Bridge/ECG/EMG/EEG AmplifierSAE-100 Small Animal ECG Amplifier SAE-200 Small Animal ECG AmplifierETH-401 4 Channel Transducer Interface

Data Recording Options:

IX-300 seriesIX-400 series

iWorx 300 Series Research Grade Recorders

The iWorx 300 SeriesResearch GradeRecorders come in 4,8 and 16 channelconfigurations. Thesesystems provide thehigh resolution andsensitivity required forcardiovascular andhemodynamic research as well as organ and tissue bathstudies, Oocyte clamp and epithelial voltage clamp applications.All 300 series recorders include stimulator outputs for each inputchannel, LabScribe2 Data Acquisition and Analysis Software forWindows and Macintosh computers, USB Cable and manuals.The recorders are available with or without galvanic isolation.

iWorx 300 Series Part NumbersIX-304Ti (isolated with transducer amplifier)IX-304T (with transducer amplifier)IX-308Ti (isolated with transducer amplifier)IX-308T (with transducer amplifier)IX-316Ti (isolated with transducer amplifier)IX-316T (with transducer amplifier)

iWorx 400 Series Recorders

iWorx 400 series areeconomical, high performance4, 8 and 16 channel datarecorders for use with a broadrange of amplifiers. Therecorders offer 16-bitresolution at maximum datacollection speeds up to 10KHz, making themappropriate for most researchapplications. All 400 seriesrecorders include LabScribe2Data Acquisition and Analysis Software for Windows andMacintosh computers, USB Cable and manuals. The 408 and416 recorders include a low voltage stimulator output.

iWorx 400 Series Part NumbersIX-404IX-408IX-416

Recorders

iWorx offers rugged, high speed, high resolution data acquisition systems suitable for teaching and research applications.

iWorx recorders include easy-to-use LabScribe2 data acquisition software in both a teaching and research version. LabScribe2 forTeaching is easy-to-use and includes courseware for more than 125 exercises in cardiovascular, respiratory and neuromuscularphysiology. LabScribe2 for Research allows advanced analysis capabilities as well as scripting.

All recorders are protected by a three-year warranty.

InputNumber of Inputs 4, 8 or 16, with or without galvanic

isolationInput Range + 10 VDC

Stimulator OutputsNumber 4, 8 or 16Resolution 16 BitOutput Range + 10 VDCModes Pulse, Train, Constant, Step,

Ramp,Triangle

A/D ConverterSample Speed up to 5 kHzInterface USB 1.1 / 2.0 full speedPower 120 V/220 V, 50/60 Hz

InputAnalog Inputs 4, 8 or 16Input Range + 10 VDC

Stimulator OutputsNumber 1Resolution 16 BitOutput Range + 10 VDCModes Pulse, Train, Constant, Step, Ramp,

Triangle

A/D ConverterSample Speed 10 kHz simultaneously (404)

100 kHz aggregate (408, 416)Interface USB 1.1 / 2.0 full speedPower USB

300 Series Recorders

400 Series Recorders

Recorders

Transducer InputsNumber of Transducer Inputs: 8Input Range: + 10 VDCResolution: 16 BitIsolation: NoExcitation: + 5 VCD, 100 mAConnector: DINGain: Programmable with

Input Resistor

Biopotential InputsNumber of Biopotental Inputs: 2Input Range: Software ConfigurableResolution: 16 BitHigh Pass Filters: Software ConfigurableLow Pass Filters: Software ConfigurableIsolation: 3 kVConnector: AAMI Safety

High Voltage Stimulator OutputNumber: 1Resolution: 12 bitConnectors: HV SafetyOutput Range: 0 - 20 mACompliance: 150 VDCMax. ON Time: 10 msec.

Low Voltage Stimulator OutputNumber: 1Resolution: 16 bitConnectors: BNCOutput Range: + 10 VDCModes: Pulse, Train, Constant,

Step, Ramp, Triangle

A/D ConverterSample Speed: 100 kHz Aggregate

InterfaceUSB: 1.1/2.0 Full Speed

Power: 5 VDC 2.6A

Enclosure: Plastic

Warranty: Protected by 3 year warranty

Software: LabScribe2 Recordingand Analysis Software

iWorx 228S Recorder

The iWorx 228S Recorder has 10 input channels and both alow voltage and a high voltage stimulator output. Channels 1and 2 can be configured for isolated recording of biopotentialsor as DC coupled inputs. Channels 3 through 10 each include atransducer amplifier. These recorders exhibit the high resolutionand low noise required for small animal cardiovascularresearch, Oocyte clamp and high end metabolic researchapplications. The IX-228S Recorder includes LabScribe2 DataAcquisition and Analysis Software for Windows and Macintoshcomputers, USB Cable and manuals.

Part NumberIX-228S

IX-228 Recorder

Components

Amplifier/Signal Conditioner Options

ETH-256C Two Channel Combination Bridge/ECG/EMG/EEGAmplifierA high performance, general-purpose research amplifier with highinput impedance and high CMR toallow reliable low noise recording ofbiopotential (ECG,EMG,EEG)signals, as well as outputs fromstrain gauge type transducers, suchas force, displacement andpressure.

ETH-401A dedicated bridge amplifier foriWorx-brand transducers thatprovides four channels of excitationpower, gain, and noise filtration. Itexhibits low system noise and offset,making it suitable for use with highresolution data acquisition systems.

SAE-100 and SAE-200 SmallAnimal ECG Amplifiers3-lead signal conditioner optimizedfor high quality, low noise recordingof small animal (rat and mouse)ECG. The SAE-100 has a DINconnector and is powered by the300 series recorder. The SAE-200has a BNC connector and includes an external power supply.

ETH-256C

ETH-401

SAE-200

Complementary Products

Pulse Oximeter for AnimalsPO2-300D Pulse Oximeter measures bloodoxygen saturation (SpO2) in animals using alingual or animal wrap sensor. Sensors aresold separately.

Sensors: PO2-200-AWC Animal Wrap SensorPO2-200-ALC Animal Lingual Clip Sensor

Animal Respiration MonitorRM-204 (Animal) RespirationMonitor with BNC connectoremploys piezo technology to followthe relative depth and frequency ofbreathing. The measuring elementis mounted in a belt that strapsaround the chest of the subject.

Temperature SensorThe TM-100 is a self-containedtemperature transducer with arange of 0o C to 50o C. The sensoris a rugged epoxy-sealed pelletmeasuring only 3.0 mm in lengthand 1.0mm in diameter. The smallmass of the sensor gives the TM-100 a fast response time. Thesealed enclosure makes cleaningand/or sterilization easy. The TM-100 requires a transducerconditioning amplifier such as the ETH-256 or ETH-401 or aniWorx computer interface equipped with transducer inputs.

TM-100

PO2-300D

RM-204

Want to Learn More?Contact us by email at info@iworx.com orby phone at (800) 234-1757.

Data Acquisition for

the Life Sciences

LabScribe is a trademark and iWorx is a registered trademark of iWorx Systems, Inc. ©2009 iWorx Systems, Inc.

iWorx Systems, Inc.

One Washington Street, Suite 404

Dover, New Hampshire 03820

(T) 800-234-1757 / 603-742-2492

(F) 603-742-2455

info@iworx.com

www.iworx.com

Cardiovascular Research Systems

Tissue Bath SystemsiWorx offers two, four, and eight channeltissue bath systems. Systems are providedwith stands, tissue tensioners, heatedcirculating pumps, and everything youneed to set up an organ bath. Thechambers are available in 5, 10, 25, and50 ml sizes. The iWorx Web-site allowsyou to customize the system that bestmeets your needs by selecting theappropriate tensioner, tissue bath, tissueholder, data acquisition system,transducer, stimulator, and heatingcirculator.

Myograph SystemsiWorx offers offers 4-channel (M4) orsingle-channel (M1) Myograph systemsfrom Radnoti Glass Technology. Thesesystems are designed for researchersperforming contractile force studies onsmall ring samples with sizes ranging from60 µm to over 1 mm in diameter.Examples include mouse aortic rings andsmall intestinal ring samples as well as micro-vessel preparations like mesentericarteries.

The Myograph systems include myographchambers with transducers and amplifiers,a base with sliding wrist rest, temperaturecontrollers, stands, tubing kits and all otheressential items to conduct an experiment.

iWorx offers a myograph normalizationmodule (LS-20NM) which calculates theoptimal pretension settings for eachsample prior to conducting an experiment.

Blood Flow SystemsiWorx offers a range of blood flow metersand probes through a distributionarrangement with Transonic Systems.Transonic Systems’ transit time technologyhas become the gold-standard in volumeflow measurements. Probes range in sizefrom several centimeters in diameter toless than 1 mm in diameter and can beused to accurately measure flow in largeanimals (renal arteries, aortic flow,coronary flow) as well in small animals(cardiac output in mice and rats).

Flow data can be acquired and analyzed inLabScribe2 providing measurements ofstroke volume, heart rate, flow rate, meanflow, frequency, derivatives and integrals.