IVF clinic Products

8/14/2019 IVF clinic Products

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ani Scientific Industries (P) Ltd.

//www.shivaniscientific.com/ivfproducts/spermfuge.htm[5/13/2009 1:50:55 PM]

Mobile Nest

Lab + GuardWarming Blocks

Turnkey Projects

Rapid Recovery

System

IVF Division

IVF P roducts Spermfu

Click here for Clinical Research paper on SpermFuge

Concept

Spermfuge is an improvised centrifuge, dedicated totally for elevating the to

motile sperm recovery from the parent semen sample, by controlling/maintain

the main parameter of "Temperature" thus aimed at Enhancing the ART results. T

instrument has been designed to regulate and subsequently maintain the "Critic

inner chamber temperature before, after and during centrifugation. Maintenance

this critical temperature throughout the entire sperm processing procedure helps t

sperm maintain their peak motility and enhanced linearity. The two of the most v

parameters which act as a yard stick to gauge the effectiveness of an ideal spe

wash. The existing dependency on RPM will be eliminated by on screen g-fo

selection.

Design

The Spermfuge has been designed after taking into consideration detailed cellu

physiological demands, locomotion kinetics and pooling together ideal suggestion

opinions, ideas and propositions of numerous Andrologists and Embryologists a

other professionals in the field of Human Reproduction. The Spermfuge with

Temperature controlled chamber (room temperature to 42C), programma

microprocessor based controls, multiple tube selection, accurate g-force selectio

independent centrifugation and/or heating selection and other added advantagmake it an ideal state of the art instrument for use in an ART laboratory.

Temperature and Centrifugation

The heating range of Spermfuge's chamber is from 2C above ambient to 42C. T

intelligent control system does not allow the centrifugation to commence till the

temperature is attained. However, one does have the option to commence t

centrifugation without chamber heating and to commence the chamber heat

without centrifugation.

Multiple Programs


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The Spermfuge facilitates user selectable 9 programs for different applications a

also for different set of protocols as standardized by various clinics, laboratories a

regulatory bodies. The user can select RCF, RPM, Tube Size, Temperature, Time

each program by a digital encoder.

Acceleration and Braking

An adjustable brake is provided to prevent the rotor from stopping too quickly

the end of run which might cause disturb to the firmly formed pellet. One c

select 3 mode in acceleration and braking, slow medium and to fast can be givon screen.

G - Force

The exact indication of G - Force helps the ideal sperm pellet formation

subjecting the sperm to an exact and precise amount of G - Force unlike ot

Centrifuges where G - Force is subject to RPM and the tube size. Now a da

centrifugation speeds are never reported in RPM as those numbers are not use

for any one wanting to reproduce the method. (Being dependent Rotor radius.)

Rotor Head and Tube Selection

Spermfuge has a swing out rotor head to accommodatetubes maximum of 15 ml. The Sperm Fuge c

accommodate 6ml Tubes (Falcon 2003), 14 ml Tub

(Falcon 2001) and 15 ml Tubes (Falcon 2095). The tu

selection can be done on screen with the help of t

encoder, which ensures an exact G - Force selection.

Operating Security

The Vision port in the lead at the center helps to confirm

rotational speed with a stroboscopic device. The safety

interlock in the lead prevents accidental opening once the

Rotor is operational. The maximum RCF is 800g which

prevents accidental damage to the sperm.

Intelligent Control System

State of the art micro-controller with optomagnetic encod

provides intelligent control system. Digital programm

via microprocessor control for various parameters includ

temperature, RCF and time. The parameters are display

on four line LCD display.

Maintenance

The Spermfuge is virtually maintenance free since it utilizes brushless drive mot


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The motor is extremely smooth even during running at the maximum speed. T

motor is electrically controlled during the acceleration and breaking w

smoothness.


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//www.shivaniscientific.com/autoclavesteriliser/frontload.htm[5/13/2009 1:51:12 PM]

Double Wall

AutoclaveLaboratory Autoclaves

Semi Automatic

Portable Autoclave

Semi Automatic

Gas cum Electric

Autoclave

Horizontal Sterilizer

Fully Automatic

Autoclaves GMP

Compliant Series

Acculeak Detector

Autoclaves & Sterilisers Front Loading Flash Sterlis

Model : FL 17

Features:

Table top front loading autoclave

Fully Automatic micro-processor based control

Sterilization chamber made up of thick stainless steel sheet, tested for h

pressure

Digital timer for both wet and dry cycle

Auto drain of water to reservoir tank & also condensation of steam

Alarm after the total command is completed

Cycle time 10-15 minutes
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//www.shivaniscientific.com/ivftraining/index.htm[5/13/2009 1:51:18 PM]

Embryology Academy of research Training (EART), a part of Shivani ScientInductries, is a Young Emerging New Dimension in the field of ART Education. EA

initiated sincere and honest efforts to understand current needs of t

embryologists & gynecologists for learning various ART techniques (IUI, IVF, IC

and Crayopreservation).

Thus, EART provides a platform for perfecting Assisted Reproductive skills & Late

technologies.

EART conducts various hands on courses in India with the help of renowned Indi

and International faculties. EART has successfully ensured that each participant ge

an adequate hands-on experience and project mock cycles, coupled with trequisite theory lectures. One of the basic criteria of learning is getting an actu

hands-on experience. The hands-on session details are mentioned in the da

sheets of each course.

One-on-one training offers the maximum opportunity to learn at EART. Courses a

available for all assisted conception centre staff including medical, scienti

personnel. The unique one-on-one training makes the courses flexible in the cour

content and the timing to meet your specific needs.

Apart from the above, EART also offers other services such as:

Turn key projects for IUI, IVF and ICSI Labs.

Calibration Services.

Quality Management System Implementation.

Accreditations

Embryology Academy for Research & Training (EART) is a recognized resea

institute for the purpose of providing the means of instruction and research to

M.V.Sc and Ph.D studies of the Maharashtra Animal and Fisheries Scien

University, MAFSU. EART is also collaborated with www.FertAid.com, Internet To


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//www.shivaniscientific.com/ivftraining/index.htm[5/13/2009 1:51:18 PM]

for the Reproductive Sciences, Australia.

For more details kindly visit www.ivftraining.com
http://www.ivftraining.com/http://www.ivftraining.com/


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//www.shivaniscientific.com/ivfproducts/mobilenest.htm[5/13/2009 1:51:46 PM]

Sperm Fuge

Lab + GuardWarming Blocks

Turnkey Projects

Rapid Recovery

System

IVF Division

IVF P roducts Mobile Ne

Concept

Human oocytes are extremely sensitive to transient cooling 'IN VITRO' and mode

reductions in Temperature can cause irreversible disruption of the meiotic spind

with possible chromosome dispersal. Embryos subjected to such heat shocks ha

high proportion of chromosomal abnormalities and this may contribute to the hi

rates of pre-clinical and spontaneous abortion. Therefore, temperature control is

paramount importance and should not be compromised in any way right from Ovu

Pick Up (OPU) till Embryo Transfer (ET).

Design

This is a handheld device with Li-ion rechargable battery. It is equipped w

heating element, Digital sensor, microcontroller, non-volatile memory and LE

indicators. When placed on base station, it gets conected electrically by

contacts. These contacts help base station and the mobile device communicat

with each other and also charge the battery. The mobile nest when in mobile mod

continuously monitors the temperature with a 14 bit accuracy, corrects it seve

times a second.

This yields a rock steady temperature profile even in varying surroundings. T


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mobile nest continues to log for the power consumption index and temperat

abnormalities. These are downloaded to the base station when the nest is dock

With monitoring and reporting features the user can be assured about the mob

nest working accurately at every step. A transparent window facilitates easy view

of the follicular fluid.

How Safe are P reheated Tubes?

The Falcon Tubes (2001) pre heated by regular Test Tube Warmer loses

temperature in less than 15 seconds the moment it is out of the heating block a

the tube reaches near to the room temperature, thus increasing the risk of therm

shock to the oocytes during aspiration. Mobile Nest is Portable Test Tube Warm

with it's own Internal rechargeable Battery which gives immense mobility and at

same time maintains precise temperature.

Intelligent Control System

State of the art microcontroller with optomagnetic encoder provides intellige

control system. Digital programming via microprocessor control for vario

parameters including temperature and power status. The parameters are display

on four line LCD display

Unique Portability

This Tubenest has been designed keeping in mind its portable nature. T

rechargeable battery is capable of running for 30 minutes at a stretch before

indicates low battery level. The unique locking mechanism for tube allo

Embryologist to pour the aspirated follicular fluid into petridish for oocyte scann

very easily. Easy grip design allows convenient handling and battery time facilita

free movement in the LAB and OT.

Oprating Security

To ensure maximum oocyte survival without quality compromise, this unit

various safety features. The Nest Tube has RED LED INDICATOR which flashes wh


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battery is weak. When the Nest is replaced in the Base Station, it checks the

and indicates any overshoots. Moreover the Nest has a safety over temperature

out.


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//www.shivaniscientific.com/ivfproducts/labplusguard.htm[5/13/2009 1:52:02 PM]

Sperm Fuge

Mobile NestWarming Blocks

Turnkey Projects

Rapid Recovery

System

IVF Division

IVF P roducts Lab + Gua

Concept

The LAB + GUARD is developed to improve the laboratory air quality making

nearly equal to clean air. Thus replenishing and subsequently nourishing the ent

laboratory, making it safe for the sensitive procedures to be executed. This

achieved by a number of inclusions and their applications all of which are simp

cost effective and user friendly. The positive pressure system uses the ba

principle of"Over P ressure or positive pressure in a room which restricts t

entry of outside air " . This is achieved by absorbing the circulating air outside th

IVF Lab (Room) and then subsequently passing the same through a series o

stage filters thus making it quite IVF friendly. Thus ultimately the effecti

circulating air in the IVF lab reaches a near clean air status, provides a posit

pressure, reduces the CFU count, eliminates the SPM, VOCs and CACs.

Ensure Net Flow Out

In any HVAC / air flow design it is essential that net flow is going out of the roo

This means room air volume and pressure should be slightly higher than adjoin

areas / rooms. This will keep out any toxic fumes trying to come in. IVF labs wh

are not having adequate positive pressure are suceptible to risk of contamination.

Design

The LAB + GUARD has been designed with State of the Art technology usingstage filtration system. The unique filter combination filters out air and g

contaminant thus by giving most suitable air for gametes and embryos. LAB

GUARD system contains a unique 4 stage filtration system containing Prefilter, He

Filter and unique blend of activated carbon and alumina, which ensures w

variety of Gases, particular contaminants are oxidized or are absorbed. The LAB

GUARD has been designed to reduce the microbial air flora to the minimum level.

Reduces CFU, Reduces VOC

The unique design and the concept of LAB + GUARD positive pressure system no

only reduces VOC content in the air but also the CFU count in the IVF Lab. Th


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LAB + GUARD not only protects embryos and gammas but also protect

embryologists who is the most affected otherwise.

Operating Security

Every care has been taken in designing to make sure that air absorbed

thoroughly cleaned for contaminants and to reduce bacterial count in IVF Lab. T

LAB + GUARD design also facilitates introduction of fresh air into the air circ

which gives maximum safety from Carbon dioxide released into the IVF Lab by C

Incubators and also it protects embryologists from gas mixtures released on wo

stations.

Change Filters As P er Usage

The LAB + GUARD also houses a unique automatic indication system whereby t

user is informed when to change the filters, and not "every quarterly". This featu

not only gives tremendous relief to the user for tracking the stipulated time

change but also saves lot of money.

MaintenanceThe LAB + GUARD has automatic indication for Filter change and the des

facilitate extremely easy replacement of filters even by non technical staff. Oth

than filter change it is virtually maintenance free

Return Blower

The return blower is utilised to circulate back the air of IVF lab to room where L

+ GUARD has been fitted.

Upgrading, Expanding Or Setting Up New IVF Lab?

The LAB + GUARD team helps you in evaluation of possibilities to improve

quality in your lab. If you are upgrading, expanding or setting up new lab cont

us for technical assistance.


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//www.shivaniscientific.com/ivfproducts/warmingguard.htm[5/13/2009 1:52:10 PM]

Sperm Fuge

Mobile NestLab + Guard

Turnkey Projects

Rapid Recovery

System

IVF Division

IVF P roducts Warming Bloc

Are you giving thermal shocks to gametes and embryos?

Sudden and drastic temperature losses, recovery times and change in pH a

minimized when using Fornax Warming Blocks. The unique aluminum alloy condu

heat and provides almost direct thermal contact with the heating surface. Dishes

room temp when kept for incubation without the Warming Block take a prolong

time to attain the set temperature compared to those dishes kept in preincubat

Warming Block, also ensures a safe and easy handling of dishes and test tubes

IVF laboratory.

GB-4WDRAMade from Aluminum Alloy with hard anodised surface.

Holding capacity 4 well NUNC 176740

Dimensions 76 x 98 x 15 mm

Weight 112gm.

GB-PDRA

Made from Aluminum Alloy with hard anodised of surface

Holding

capacity

One PetriDish Falcon 3002 /

3037

Dimensions 76 x 98 x 15 mm

Weight 160 gm.

GB-717CA

Made from Aluminum Alloy with hard anodised surface.

Holding capacity 7 tubes of 14 ml / 17 mm

Dimensions 83 x 80 mm(d x h)

Weight 888 gm.

GB-IDHA

Made from Aluminum Alloy with hard anodised surface.

Holding capacity One ICSI dish - Falcom 1006

Dimensions 83 x 8 mm (d x h)


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Weight 44 gm.


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//www.shivaniscientific.com/ivfproducts/turnkeyproducts.htm[5/13/2009 1:52:21 PM]

Sperm Fuge


Warming Blocks

Rapid Recovery

System

IVF Division

IVF P roducts Turnkey Proje

Shivani Scientific Industries (P) Ltd has vast experience of more than 20 years

designing and building pharmaceutical Laboratories as per WHO-GMP norms w

stringent requirement of sterility. The same experience has helped us to fulfill t

demanding needs of to-days IVF Labs.

A new Lab means start contacting designers, architects, sellers, resellers, vendo

suppliers, agents, distributors to initiate slow, long and laborious process

discussing dates and negotiating rates. This, you will agree, will further eat i

your most precious commodity "Time".

Alternatively you have the easier and simpler option: Contact US.


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//www.shivaniscientific.com/ivfproducts/turnkeyproducts.htm[5/13/2009 1:52:21 PM]

3D Lab design / 3 D W alk through

We offer State of the Art computer modeling to visualize your Lab either in 3D

3D Walk through, even before your lab is ready.

Customised Video Processing

All modern equipments comes with all provision of image processing which is ve

well utilized by our Combination of hardware camera selection and softwa

selection. Multiple Camera inputs can be synthesised in one system to have bet

recording and processing.

Embryology Services

We provide assistance in providing complete embryology services from Ovum Pic

up to embryo transfer. Qualified and experienced team of clinicians a

embryologists are selected to provide these services.

Air Quality evaluation and improvement

We have capability to thoroughly evaluate Air quality on existing Lab a

recommend appropriate steps to improve the same. It varies from HVAC inspect

to microbial air sampling.

Hands on training for Lab Staff

We offers comprehensive hands on training in collaboration with Maharash

Animal & Fishery Sciences University to Lab Staff.

Annual Maintenance of IVF Lab Equipments

We undertake Annual Maintenance Contracts for IVF Labs with the support of

highly qualified team of Engineers. We also cater to calibration contracts with o

highly accurate calibration equipments.


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//www.shivaniscientific.com/ivfproducts/rapid-recovery-system.htm[5/13/2009 1:52:30 PM]

Sperm Fuge


Warming Blocks

Turnkey Projects

IVF Division

IVF P roducts Rapid Recovery Syste

What is Heat Transfer?

To define Heat Transfer requires 2 important group of words. It is a form of enerand in that context we are concerned with the transport of heat energy from o

body to another. Motivation for this transfer is provided by the difference

temperature. The definition of Heat Transfer would therefore include a wo

transport of energy due to temperature difference.

Conduction vs. Convection

The word Convection means to bring in or to carry. In other words in normal C

Incubator the heat energy is carried through the medium of air to the Petridish

well dish and thereafter it is transmitted to culture media and then to embryo

Whereas Conduction means transfer of heat energy through direct contact of so

substances.

In this case the heat energy in the incubator all the heated shelf can be direc

transmitted to the 4 well dish or Petridish if they are in contact.

Thermal Conductivity

Thermal Conductivity of air is 0.034 whereas Thermal Conductivity of Aluminium

204 and Thermal Conductivity of Stainless Steel is 19.

Temperature changes during embryo culture when the dishes are taken out fro

37 C incubator into lab where the temperature is anywhere between 24 C to 2


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//www.shivaniscientific.com/ivfproducts/rapid-recovery-system.htm[5/13/2009 1:52:30 PM]

C and also when culture media which are used in embryo culture lose t

temperature when they are droplet from flat surface.

Temperature changes recovery time and PA changes are drastically reduced wh

Rapid Recovery System is used with regular CO2 Incubator. The new Ra

Recovery System provides direct contact, which promotes heat further throu

conduction to bottom of the culture dish.

Loss of temperature on heated surfaces

The air gap under culture dishes promotes bad conduction between incubator sh

and the bottom of the dishes and as air is very bad conductor of heat it is qu

deter mental to the embryos and gametes.

Recovery of CO2 Incubator

When the dishes are kept back into the CO2 Incubator, CO2 often shows recove

of the temperature on the display. However, it does not mean the temperature

Embryos and Oocyte has reached. It is often reported that the actual stabilizat

on 37 C in the Embryo happens after 22-30 minutes of Incubator.

The New Rapid Recovery System

The New Rapid Recovery system has been designed to enhance Embr

development and the culture conditions by recovering temperature practically fas

than conventional methods. The new Rapid Recovery System is to be used with o

GB Series Warming Blocks. The Rapid Recovery System has necessary desig

feature to accommodate 4 well dishes, Nunc Dishes and Falcon Petridishes. T

Rapid Recovery system give you direct thermal contact between the dry block ty

heating system and the individual culture dish wells, ensuring rapid temperatu

equilibration combined with low temperature variability.


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//www.shivaniscientific.com/ivfproducts/ivfproducts/index.htm[5/13/2009 1:52:44 PM]

Sperm Fuge


Warming Blocks

TurnKey Projects

Rapid Recovery

System

IVF P roducts IVF Division

Click here to know more Click here to know more






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1

Dr Franken, K Avari

February 2007

Introduction

Sperm retrieval techniques form an integralpart of the assisted reproductive programme.The success of sperm separation is measuredby the number of motile sperm retrieved from agiven semen sample. The influence of varioustechnical procedures and diluent media uponhuman spermatozoa has been tested in vitro(Jeulin et al., 1982). The percentage andvelocity of motile spermatozoa are oftenmeasured objectively using laser Dopplervelocimetry or Hamilton Thorne IVOS 10computerized sperm analyzer (CASA). Whenspermatozoa are incubated at 37 degrees C,rapid declines in both percentage motile and in

velocity were observed with incubation periodslasting more than 4-h (Jeulin et al., 1982).Human sperm incubation at room temperaturedoes not allow capacitation, although it doesnot affect hFF-induced acrosome reaction incapacitated cells. The blocking effect isovercome when spermatozoa are exposed to37C (Maryn-Briggiler et al., 2002). Keppler etal., (2002) found significantly higher percentmotility, mean average path velocity, straightline velocity, lateral head displacement, andpercent hyperactivation in sperm at the 40Ctemperature (Keppler et al., 1999)

Objectives

To evaluate the effect of temperature on thesperm motion characteristics and subsequentoutcome of sperm preparation.

Material & Methods

Semen samples and sperm preparation

Semen samples were obtained fromnormozoospermic donors and andrologyreferrals. Directly after collection samples weredivided into 2 aliquots. One aliquot wereplaced in a CO2 incubator at 34

0C while the

second aliquot were left at room temperature.A period of 30 minutes was allowed to stabilizethe semen temperatures before experimentalprocedure. Prior to the experimental onset themotion characteristics were recorded with theCASA instrument in order to obtain initialvalues for the measured motioncharacteristics.

Swim up separation

Motile sperm fractions were retrieved from thesemen samples by mixing 0.5mL semen with1mL of Quinns Sperm Preparation medium.Two tubes were prepared for each experiment.The tubes were then placed in 2 differentcentrifuges, namely (i) SpermFuge SF 800(Shivani Industries, Mumbai, India), a highlyprecise centrifuge with a temperaturecontrolled chamber at 34

0C and (ii) Sigma

bench top with no temperature controlfacilities. Both centrifuges were set at1500rpm (428xG) for 5 minutes. Followingthe second washing procedure, both spermpellets were layered with culture medium andleft at a 45

0angle at 34

0C for 60 minutes. After

the incubation period 0.5mL supernatant wereremoved from each tube and immediatelyanalyzed for sperm motion characteristics.

Temperature Controlled Centrifugation: -The Effect on Motion Characteristics of Human Sperm

Department of Obstetrics & Gynaecology,

University of Stellenbosch, Tygerberg Hospital,Tygerberg, 7505, South Africa


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Temperature Controlled Centrifugation

2

Motion characteristics

Aliquots of 5L of spermatozoa (10x106cells/mL) from the sperm at 34C and 22Caliquots were placed in the micro chamber foranalyses with the HTM-IVOS V10.9 CASA

instrument (Hamilton-Thorne Research Inc.,Beverley, MA, USA). The following standardset-up parameters: 30 frames/60 Hz; minimumcontrast, 80; minimum cell size, 2; minimumstatic contrast, 30; low VAP cut-off, 5 m/s;low VSL cut-off, 11 m/s; head size, non-motile, 3; head intensity, non-motile, 160;static head size, 1.01-2.91; static headintensity, 0.60-1.40; slow cells, non-motile;magnification, 2.01; and temperature, at 34C.

The following parameters were evaluated:sperm concentration; motile and progressivelymotile concentrations; percentage motile andprogressively motile; path velocity (VAP);straight-line velocity (VSL); curvilinear velocity(VCL); amplitude of lateral head displacement

(ALH); beat cross frequency (BCF);straightness (STR); and linearity (LIN). Motioncharacteristics were recorded in all samplesusing 10 randomly selected microscopic fields.The proportion of hyperactivated spermatozoain each experiment was determined using theSORT function of the CASA instrument. To beclassified as hyperactivated, a trajectory had tomeet all of the 60 Hz SORT criteria, i.e.,VCL150m/s, LIN50% and ALH7m(Mortimeret al., 1990).

Results

Table 1 Results of semen parameters recorded in ejaculates and after preparationwith SpermFuge temperature controlled vs. room temperature centrifugation

Post Swim up

Ejaculates34

0C Spermfuge Room temperature

Volume

mL

Morphology% normal

Motile

% live

SpermConcentration

Mill/mL

Spermconcentration

Mill/mL

SpermConcentration

Mill/mL

Mean 3.20 10.80 58.00 80.60 12.10 a 7.30 b

SD 1.15 3.04 24.40 53.14 6.63 2.38

a vs.b Fishers exact test p =


22/22

Temperature Controlled Centrifugation

DFR22022007-F3

Table 3 Comparison of the velocity distribution of sperm cells( %) prepared by a SpermFugetemperature controlled (34

oC) centrifuge vs. room temperature swim up spermatozoa

SPERM VELOCITIES DISTRIBUTED IN 5 MOTION CATEGORIES

% RAPID % MEDIUM % SLOW % STATIC % HA

Baseline

34oC

20oC

Baseline

34oC

20oC

Baseline

34oC

20oC

Baseline

34oC

20oC

Baseline

34oC

20oC

Mean 4.2 4.5 4.8 3.2 3.5 3.6 4.0 3.6 3.3 4.0 3.6 3.9 0.4a 1.2b 0.3c

SD 0.7 0.7 1.3 0.5 0.7 0.6 0.5 0.6 1.0 0.7 0.4 0.7 0.8 1.2 0.8

Transformed ARCSIN values showed a vs.b, a vs c and b vs. c Fishers exact test p=

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