IV.Bacterial Structure and Growth A.Bacterial Cells: An Overview B.Bacterial Cell Structures...
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IV. Bacterial Structure and Growth
A. Bacterial Cells: An Overview
B. Bacterial Cell Structures
C. Factors that Influence Bacterial Growth
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IV. A. Bacterial Cells: An Overview
• Shapes & Arrangements– Round Bacteria
Coccus Staphylococcus
Diplococcus Tetrad
Streptococcus Sarcina
– Rod-shaped Bacteria
Bacillus Streptobacillus
Diplobacillus Coryneform bacteria
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IV. A. Bacterial Cells: An Overview
• Shapes & Arrangements (cont.)– Curved & Spiral Bacteria
Vibrio
Spirillum
Spirochaete
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IV. A. Bacterial Cells: An Overview
• Sizes– Typically ~ 0.1 - 20 m (with some exceptions)
– Typical coccus: ~ 1 m (eg Staphylococcus)
– Typical short rod: ~ 1 x 5 m (eg E. coli)
– Barely within the best resolution of a good compound light microscope
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IV. A. Bacterial Cells: An Overview
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IV. B. Bacterial Cell Structures
1. Capsules
2. Cell Wall
3. Plasma Membrane
4. Cytoplasm & Cytoplasmic Inclusions
5. Ribosomes
6. Bacterial DNA
7. Pili
8. Flagella
9. Spores
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IV. B. 1. Capsules
• Species and strain specific
• Structure – Polysaccharide or polypeptide layer outside cell
wall
– May be tightly or loosely bound
– Detected by negative staining techniques
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IV. B. 1. Capsules (cont.)
• Functions – Attachment
– Resistance to desiccation
– Nutrient Storage
– Evasion of phagocytosis
eg. in Streptococcus pneumoniae
S strain is encapsulated & virulent
R strain is nonencapsulated & nonvirulent
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IV. B. 2. Cell Wall
• Gram Staining– Method developed by Gram in 1888
– Gram-positive cells stain purpleGram-negative cells stain pink
– Later, it was discovered that the major factor determining Gram reactions is the bacterial cell wall structure
– “Gram-positive” & “Gram-negative”These terms can mean either:Staining results, or Types of cell wall structure
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IV. B. 2. Cell Wall
• Peptidoglycan Structure– Composition
• A Polysaccharide
• Composed of alternating units ofN-acetylglucosamine (NAG) andN-acetylmuramic acid (NAM)
– Peptide Crosslinking BetweenNAM units
– Much thicker and more crosslinking in Gram-positive than in Gram-negative Bacteria
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IV. B. 2. Cell Wall
• Gram-positive Cell Wall– Thick Layer of Highly Crosslinked
Peptidoglycan
– Teichoic Acid Strands
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IV. B. 2. Cell Wall
• Gram-negative Cell Walls– Outer Membrane
• Lipopolysaccharide Layercontaining Lipid A
• Phospholipid Layer
• Outer Membrane Proteins
– Thin Layer of Peptidoglycanwith no teichoic acid
– Periplasmic Space
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IV. B. 2. Cell Wall
• Variations on Cell Wall Architecture– Acid-fast Cell Walls
• Similar to Gram-positive structure, buthave Mycolic Acid: A waxy lipid
• Require special acid-fast staining technique
• Includes Mycobacterium and Nocardia
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IV. B. 2. Cell Wall
• Variations on Cell Wall Architecture (cont.)– Mycoplasmas
• Bacteria that are naturally have no cell walls
• Includes Mycoplasma and Ureaplasma
– Archaeobacteria• Have unusual archaeobacterial cell walls
with no peptidoglycan
• Have unusual metabolisms
• Share a more recent common ancestor with eukaryotes than with eubacteria (“true bacteria”)
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IV. B. 3. Plasma Membrane
• Structure– Phospholipid Bilayer with Associated
Proteins
• Functions – Maintain Cell Integrity
– Regulate Transport
– Specialized Functions in Bacteria
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IV. B. 4.Cytoplasm & Cytoplasmic Inclusions
• Composition: – Viscous aqueous suspension of proteins,
nucleic acid, dissolved organic compounds, mineral salts
• Cytoplasmic Inclusions:– Metachromatic Granules (Phosphate)
– Starch Granules
– Lipid Droplets
– Sulfur Granules
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IV. B. 5. Ribosomes
• Suspended in Cytoplasm
• Sites of Protein Synthesis
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IV. B. 6. Bacterial DNA
• Chromosomal DNA
• Plasmid DNA –R-Plasmids
–F-Plasmids
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IV. B. 7. Pili
• Hair-like structures on cell surface
• Functions –Attachment
–Conjugation
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IV. B. 8. Flagella
• Function– Motility
Almost all motile bacteria are motile by means of flagella
– Motile vs. nonmotile bacteria
• Structure– Filament
Composed of the protein flagellin
– Hook & Rotor AssemblyPermits rotational "spinning" movement
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IV. B. 8. Flagella
• Mechanism of Motility– “Run and Tumble” Movement
controlled by the direction of the flagellar spin
– Counterclockwise spin = Straight RunClockwise spin = Random Tumble
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IV. B. 8. Flagella
• Chemotaxis– Response to the concentration of chemical
attractants and repellants
– As a bacterium approaches an attractant:the lengths of the straight runs increase
– As a bacterium approaches a repellant:the lengths of the straight runs decrease
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IV. B. 9. Spores• Function
– To permit the organism to survive during conditions of desiccation, nutrient depletion, and waste buildup
– Bacterial spores are NOT a reproductive structure, like plant or fungal spores
• Occurrence – Produced by very few genera of bacteria
– Major examplesBacillus Clostridium
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IV. B. 9. Spores
• Significance in Medicine & Industry – Spores are resistant to killing
– Cannot be killed by 100°C (boiling)
– Requires heating to 120°C for 15-20 min (autoclaving or pressure cooking)
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IV. B. 9. Spores
• Sporulation – The process of spore formation
– Governed by genetic mechanism
– A copy of the bacterial chromosome is surrounded by a thick, durable spore coat
– This forms an endospore within a vegetative cell
– When the vegetative cell dies and ruptures, the free spore is released
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IV. B. 9. Spores
• Spore Germination – When a spore encounters favorable growth
conditions
– The spore coat ruptures and a new vegetative cell is formed
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IV. C. Factors that Influence Bacterial Growth
• Growth vs. Survival– Bacteria may tolerate or survive under more
extreme conditions than their growth conditions
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IV. C. Factors that Influence Bacterial Growth
• Nutrient Requirements– Energy Source
Most bacteria are chemotrophs; a few are phototrophs
– Carbon SourceMost bacteria are heterotrophs; a few are autotrophs
– Nitrogen, Phosphate, Sulfur, Trace Minerals
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IV. C. Factors that Influence Bacterial Growth
• Nutrient Requirements (cont.)– Special Requirements
examples: amino acids and enzyme cofactors (vitamins) Fastidious bacteria: Strains that are difficult or impossible to culture due to special growth requirements
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IV. C. Factors that Influence Bacterial Growth
• Temperature– Psychrophiles
Grow at ~0°C - 20°C
– Mesophiles Grow at ~20°C - 45°C
– Moderate Thermophiles Grow at ~45°C - 70°C
– Extreme Thermophiles Grow at ~70°C - 100°C
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IV. C. Factors that Influence Bacterial Growth
• pH– Acidophiles
Grow at ~pH 1.0 - pH 6.0
– Neutrophiles Grow at ~pH 6.0 - pH 8.5
– Alkalophiles Grow above pH 8.5
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IV. C. Factors that Influence Bacterial Growth
• Oxygen – Strict aerobes (Obligate aerobes)
Use oxygen for respiration in their metabolismRequire the presence of a normal oxygen concentration (~20%) for growth
– Strict anaerobes (Obligate anaerobes) Oxygen is a poison for these microbes Cannot grow at all in the presence of oxygen
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IV. C. Factors that Influence Bacterial Growth
• Oxygen (cont.)– Aerotolerate anaerobes
Do not use oxygen, but oxygen is not a poison for these Can grow equally well with or without oxygen
– Facultative anaerobes Use oxygen for respiration, but can also grow without oxygen Grow better with oxygen that without oxygen
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IV. C. Factors that Influence Bacterial Growth
• Oxygen (cont.) – Microaerophiles
Require low concentrations (~5% - 10%) of oxygen for growth