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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Venepuncture and IV Cannulation

    Medical Student

    Practical Skill Session

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Contents

    Anatomy And Physiology 3Structure Of Veins 4Superficial Veins 5Antecubital Fossa 7Veins 7Arteries 7

    Patient Assessment 11Factors Influencing Vein Choice 11Condition Of Vein 11Improving Venous Access 11Site Preparation 12Infection Control 12

    Venepuncture 13Procedure Of Venepuncture 13Equipment 13Procedure 13Intravenous Cannulation 14Patient Assessment 14Cannula Selection 14Methods To Reduce Pain 14Cannulation Procedure 15Equipment 15Cannulation 15Securing The Cannula 16Care Of The Cannula 16Complications 16Resiting Or Removal Of Cannula 16

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Anatomy and Physiology

    Structure of veins Superficial veins

    Antecubital Fossa

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Superficial Veins Of The Upper Limb

    Cephalic vein

    Median Cubital vein

    Basilic vein

    Accessory Cephalic vein

    Cephalic vein Superficial Median vein of the forearm

    Palmar Venous Plexus

    Palmar Digital veins

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    The Forearm Veins

    The Cephalic Vein

    Forms from a confluence of veins at the base of the thumb and passes upward along the radial

    (lateral) aspect of the forearm to enter the lateral part of the antecubital fossa.

    PRO's.

    Readily receives a large cannula and is therefore a good site for blood administration. Splinted by the forearm bones. Cannula is easily secured.

    CON's.

    Can be more difficult to cannulate than the metacarpel veins. May be confused with an aberrant radial artery.

    The Basilic Vein

    Forms from a confluence of veins on the postero-medial aspect of the wrist and passes upward

    slightly posterior to the ulnar (medial) border of the forearm but winds round over the ulnar to enter

    the medial aspect of the antecubital fossa.

    PRO's

    A large vein that is frequently overlooked in the hunt for veins.CON's.

    Requires awkward positioning of the limb to gain access to the vein.

    The vein tends to roll away when you attempt to cannulate it. Sites prone to phlebitis. Cannula port gets caught on sheets.

    The Median Veins Of The Forearm

    Many Veins with vary variable courses.

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Metacarpal Veins

    PRO's

    Easy to see and palpate veins. Splinted by metacarpal bones. Allows use of more proximal veins in the same limb should the cannula need to be re-sited. Cannula is easily accessible in the theatre environment.

    CON's

    Active patients may dislodge easily. Dressing may be compromised by handwashing. May be more difficult if the skin is thin and friable. Flow can be affected by wrist flexion or extension i.e. A POSITIONAL VENFLON.

    Basilic vein

    Cephalic vein

    Dorsal Venous Plexus

    Dorsal metacarpal veins

    Dorsal Digital vein

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    The Veins Of The Antecubital Fossa

    At least 3 major veins;

    Cephalic Vein

    A continuation of the vein upward from the antero-lateral aspect of the forearm onto the antero-lateral aspect of the arm over the biceps muscle. From here it passes up to the deltoid muscle where,

    at a variable point, it passes through the superficial fascia to join the brachial vein to form the

    axillary vein.

    Basilic Vein

    A continuation of the vein from the antero-medial aspect of the forearm. It may pierce the

    superficial fascia in the antecubital fossa and join the deep veins to form the brachial vein or it may

    traverse the antecubital fossa and pierce the fascia at a variable point on the medial aspect of the

    arm.

    Median Vein

    There may be more than one median vein in the antecubital fossa.

    They are formed by the convergence and divergence of branches of the 3 forearm vems.

    PRO's

    Large veins and so they will readily accept a large cannula.Do not "shut down" as quickly as the more peripheral veins.FIRST CHOICE IN THE EMERGENCY SITUATION.

    CON's

    Can be very positional due to elbow flexion/extension.Can be very uncomfortable for the patient due to elbow flexion/extension.Care must be taken not to cannulate the brachial artery.

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    The Antecubital Fossa.

    Brachial ArteryBasilic Vein

    Biceps Medial Cutaneous

    Nerve of ForearmBrachialis

    Med. Cut. N of

    Forearm and Loop

    Lat. Cut. N of Forearm

    Median N

    Brachioradialis Median Basilic Vein

    Median Cephalic VeinBicepital Aponeurosis

    Cephalic Vein Deep Communicating Vein

    Pronator Teres

    Superficial

    Median Vein

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Veins

    DefinitionA collecting system of vessels for blood RETURNING from the peripheries to the heart.All veins, except for the pulmonary veins, carry deoxygenated blood and carbon dioxide.

    There are 3 venous systems;

    Systemic:

    Drains blood from all the organs, except for the lungs and G.I. tract back to the right atrium.

    This system can be sub-divided into a SUPERFICIAL and DEEP system according to the

    veins' relationship to the superficial fascia of the body.

    Pulmonary:

    Drains oxygenated blood from the lungs to the left atrium.

    Portal:

    Drains blood from the G.I. tract between the gastro-oesophageal junction and the recto-analjunction and carries it to the LIVER. The blood then drains into the systemic system via the

    hepatic veins.

    All veins, except for the superficial systemic veins, have a similar pattern of distribution as arteries,

    e.g

    Femoral Vein and Artery

    Carotid Artery and Internal Jugular Vein (external jugular is a superficial vein).

    Structure3 layers like arteries, but;

    There is much less muscle in the media which means the wall is much thinner and ismuch more easily distended or collapsed by pressure.

    The intima is folded up to form venous valves.Despite its thinner media the vein retains significant sympathetic innervation and so significant

    VENOCONSTRICTION can occur leading to collapsed or SHUT DOWN veins.

    Arteries

    Definition The vessels carrying blood AWAY from the heart. All arteries, except the PULMONARY arteries, carry oxygenated (bright red) blood.

    Structure3 layers

    Intima: Consists of an ENDOTHELIUM surrounded by a thin layer of elastic tissue.

    The endothelial cells are flat and line the vessel to promote the smooth laminar

    flow of blood. They also release chemical substances involved in the initiation of

    clotting. More recently it has been discovered that they synthesise and releasenitric oxide, a -simple molecule, involved in many physiological and pathological

    processes.

    Media: A thick layer of intermingled smooth muscle cells and elastic fibres.

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Its function is to distend as the heart ejects blood into the arterial tree and then to

    contract back down when the heart goes into diastole. This maintains the normal

    calibre of the vessel and also promotes forward flow of blood during diastole.

    This effect can beseen on an arterial line or pulse oximeter trace as a "bump" on

    the downstroke of the trace.

    Adventitia: A tough fibrous layer.

    This protects the artery and merges in with the surrounding connective tissu

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Patient Assessment

    Factors Influencing Vein Choice

    Age of patient

    Previous uses and condition of the veins

    Clinical status of patient e.g. Dehydrated, shock, amputee, mastectomy, oedema, thrombocytopenia,CVA

    Other clinical procedures required during admission

    Type and length of treatment

    Medications: warfarin, heparin, steroids

    Patient preference

    Patient co-operation, previous experiences

    Try to use non dominant arm

    Sites: median antecubital veins, forearm veins, dorsum of hands and in difficult patients dorsum of

    foot.

    Condition Of Vein

    A good vein is:

    Bouncy Soft Refills when depressed Visible Has a large lumen Well supported Straight

    A void veins which are:

    Thrombosed / sclerosed / fibrosed Inflamed / bruised Hard Thin / Fragile Mobile / tortuous Near bony prominences, painful Areas or sites of infection, oedema or phlebitis In the lower extremities (unless none else available) Have undergone multiple previous punctures

    Improving Venous Access

    Application of a tourniquet promotes venous distension. The tourniquet should be tightenough to impede venous return but not affect arterial flow.

    Lower the extremity below the level of the heart Use muscle action to force blood into the veins - e.g. open and closing of the fist Light tapping of the vein Apply warm compresses or immerse limb in bowl of hot water to increase vasodilatation Consider GTN Patch

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Site Preparation

    Position the patient appropriately to facilitate the procedure, you may need help.

    Choose an appropriate site

    Infection Control

    Asepsis is vital as the skin is breached and a foreign object is introduced into a sterile circulating

    system. The main sources of microbial contamination are:

    Cross infection from practitioner to patient Skin flora

    Hands should be clean, having been washed prior to the procedure, and an alcohol solution/gel

    applied to the hands before donning a pair of gloves. Gloves will protect your hands against

    contamination from the patients blood, and will provide some additional protection in the case of a

    needle-stick injury by wiping some of the contaminating blood from the needle prior the skin

    puncture.

    The site of the proposed venepuncture should be wiped with an isopropyl alcohol 70% swab (e.g.

    mediswab) and this should be allowed to dry (for a minimum of 30 seconds) prior to proceeding

    with venepuncture. This will clean any gross contamination of the patients skin and will reduce the

    patients skin flora at the site of puncture.

    The skin must not be touched or the vein re-palpated once the skin has been cleaned,

    Sharps should be immediately disposed of in a sharps container, and no needles should be re-

    sheathed.

    This is to avoid needle-stick injuries to you or others involved in the patient's care, lowering theincidence of blood borne viral illnesses (In particular Hepatitis B/C and HIV)

    Use a no-touch technique for any part of the needle or cannula which is to puncture the patient's

    skin.

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Venepuncture

    Procedure Of Venepuncture

    Equipment

    Tray Mediswab Tourniquet Small adhesive dressing. Sharps Container Gloves Isopropyl alcohol 70% solution hand rub solution Vacutainer system

    needle, holder, appropriate evacuated tubes

    Or

    Sterile syringe, Sterile needle, Appropriate evacuated tube

    Procedure

    1. Assemble equipment2. Inform patient of procedure3. Select a suitable vein - e.g. the vein in the antecubital fossa or forearm4. Palpate the vessel to exclude the possibility that it is an artery5. Apply a tourniquet medial to selected site6. Put on gloves7. Cleanse skin with alcohol wipe8. Fix the vein by applying pressure to skin over the vein, approximately two inches below

    venepuncture site

    9. Leaving the coloured shield on the needle, screw it onto the holder10. Remove shield and approach the skin, with needle bevel uppermost at an angle of 35~45

    degrees

    11. When the needle has penetrated the skin, realign it with the vein and reduce the angle to about15 degrees

    12. Introduce the tube into the holder, with middle and forefmger supporting flange of the holder,push the tube with the thumb to the end of the holder, puncturing the diaphragm of the

    stopper.

    13. As soon as blood starts to flow into the tube, remove the tourniquet.14. When blood flow ceases, gently disengage tube from holder - if more samples are required,

    repeat from stage 12

    15. Tubes with additives should be gently inverted to mix contents - shaking may causehaemolysis.

    16. Always draw samples without additives first.17. Place a clean swab or piece of cotton wool over the needle as it is gently withdrawn, pressure

    should be applied to the site until haemostasis occurs, at which time an adhesive dressing is

    applied. It is not recommended that the patient bend their arm as this increases the

    intravascular pressure.

    18. Ensure all samples are clearly labelled19.

    Never re-sheath needles as this is the commonest source of needles tick injury.20. Ensure all sharps are disposed of safely and examine holder for any contamination, in whichcase it should be discarded - in normal practice the holder does not come into contact with

    blood products and is intended for multiple use.

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Intravenous Cannulation

    Patient Assessment Site Selection

    Site selection

    Inspect both arms (and legs if required)

    Try to use non dominant armPalpate the vein

    Does it bounce? Make sure it does not pulsate. Is it thrombosed?, will it take the size of cannula

    needed? Will you be able to secure the cannula easily? Does the venous drainage look normal (is

    there evidence of fracture, extravasation from previous proximal cannula, lymphoedema or

    paralysis? Have you avoided a joint area which may need to be splinted?

    Cannula Selection

    When considering the choice of cannula consideration should be given to the following: minimising

    discomfort to the patient, ensuring good flow rates, and easy insertion with no tissue reaction to the

    cannula. It should be of the smallest practical size to provide the required fluid regimen and take

    into account the size of vessel cannulated, the time scale of the proposed administration of infusion

    and the viscosity of the fluid to be infused.

    Flow through the cannula is proportional to:

    The fourth power of the radius i.e. 2xr=16xflow

    The pressure difference across the cannula (i.e. pressurised infusions flow faster)

    Flow through the cannula is inversely proportional to:

    The length of the cannula

    The viscosity of the fluid being administered

    Colour Size mm Max flow/min (length) Common uses

    Orange/brown 14g 2.0 265mllmin (l=42mm) Rapid transfusions, blood

    Grey 16g 1.7 170mllmin (l=42mm) As above

    Green 18g 1.2 90mllmin (l=40mm) IV maintenance fluids

    Pink 20g 1.0 55mllmin (l=32mm) IV drugs/infusions

    Blue 22g 0.8 25mllmin (l=25mm) Paediatrics/difficult veins.

    Methods To Reduce Pain

    Good technique, skill and vein selectionLocal anaesthetic infiltration

    Topical anaesthesia e.g. EMLA and Amethocaine gel

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Cannula Procedure

    Equipment

    IV cannula Alcohol wipe 2ml syringe 25g needle 5ml amp of 1 % lignocaine solution Adhesive dressing for fixation of cannula Tourniquet Sharps Container Gloves Isopropyl alcohol 70% solution hand rub solution

    Cannulation

    1. Assemble equipment2. Inform patient of procedure3. Select a suitable vein - e.g. the vein in the forearm or dorsum of the hand4. Palpate the vessel5. Apply a tourniquet medial to selected site6. Put on gloves7. Cleanse skin with alcohol wipe8. Infiltrate skin over proposed puncture site with 1 % lignocaine solution9. Hold patient's hand with your non-dominant hand, using your thumb to keep skin taut, and

    anchor vein to prevent it rolling

    10. Inspect needle tip to ensure cutting edge is smooth and intact. Place cannula needle in linewith direction of the vein, and a few mm below proposed entry site, with bevel pointingupwards to reduce tissue trauma

    11. At a low angle, gripping the cannula as in demonstration, insert the needle through the skinand into the vein, as identified by the flashback of blood into the chamber at the hub of the

    cannula

    12. Once inside the vein advance the needle 2-3mm in a parallel motion to ensure the cannula isalso in the vein

    13. Withdraw the needle stylet (holding the cannula steady) about 5mm to avoid piercing theposterior vein wall, there should be a further flashback of blood along the shaft of the cannula

    and now advance the cannula into the vein.

    14. Never re-insert the stylet as this can shear off the end of the cannula and cause an embolus.15. Release the tourniquet16. Place a finger over the vein above the tip of the cannula to prevent bleeding as you now

    remove the needle stylet.

    17. To separate the needle and the lure lock cap, hold the cap between thumb and third finger anduse your index finger of the same hand to push on the guard, away from you.

    18. Place the cap on the cannula and safely dispose of the needle19. Flush the cannula with heparinised saline to ensure cannula patency20. Cover the insertion site and immobilise the cannula by applying a sterile non-occlusive

    dressing.

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    University Section of Anaesthesia, Pain & Critical Care Medicine Clinical Skills in Venepuncture & IV Cannulation

    Securing The Cannula

    It is important to secure the cannula to prevent mechanical phlebitis.

    This can be done with clean tape or a special adhesive dressing. Care should be taken to avoid the

    insertion site.

    If the device is located over a joint, the joint should be immobilised and splinted to prevent

    movement and dislodgement of the cannula.

    Care Of The Cannula

    Once sited the cannula should be flushed with either normal saline or heparinised saline. The site

    should be regularly inspected for signs of phlebitis.

    Peripheral cannulae should be re-sited every 48-72 hours to reduce the risk of phlebitis, but this

    may be difficult in patients with difficult veins.

    Complications

    If Cannulation is unsuccessful do not reinsert stylet into cannula as it may shear off the cannula andlead to catheter embolism.

    Chemical irritation from the infusion may cause phlebitis and pain. An acidic pH and high

    osmolality are particularly likely to cause problems. Dilute solutions appropriately for peripheral

    administration. Where osmolarity of the solution exceeds 600molmolal avoid peripheral venous

    administration and give into a central vein. Buffering of solutions prior to administration with small

    quantities of phosphate or bicarbonate buffers up to a pH of 7 will reduce the incidence of phlebitis

    from chemical irritation but introduces the risk of making the environment more suitable for

    bacterial contamination.

    The cannula may block from thrombus formation if it is not kept flushed.

    Extravasation occurs when cannula pulls out of the vein, or becomes partly occluded by venous

    constriction causing back flow of the infusate through the puncture site into surrounding tissues.The patient may complain of tightness, burning and discomfort around the iv site and there may be

    swelling and blanching of the tissues. Treatment is to stop infusion immediately and re-site cannula.

    Haematoma is formed when blood leaks into the tissues surrounding the insertion site after failure

    to penetrate vein properly during insertion, puncture of posterior wall of vessel or removal of the

    cannula. Treatment is to apply pressure to puncture site for 3-4 minutes.

    Infection: This can cause phlebitis and thrombus formation. It is prevented by good aseptic

    technique, keeping the dressings clean and not leaving the cannula in for any longer than necessary.

    Phlebitis: This is acute inflammation of the intima of the vein. It is caused by mechanical and

    chemical irritation, or by microscopic particles that may contaminate infusion fluids. Clinically

    there is erythema over the cannulated vein and surrounding skin and it is warm to touch. Treatment

    is to remove the cannula. Thrombophlebitis. This is acute inflammation of the intima of the veinwith the formation of a thrombus.

    It is commonly associated with infection at the site of the cannula and may present with raised

    white cell count, lymphadenopathy and positive blood cultures. There may be pus visible around

    skin entry site. Treatment is to remove cannula and commence parenteral antibiotics.

    Resiting Or Removal Of Cannula

    Cannulae should not remain in situ for any longer than necessary to reduce the risks of infection.

    Consideration should be given to resiting them after 48-72 hours.

    When removing the cannulae, pressure should be applied to the site for at least a minute and the site

    should be occluded with a sterile dressing.