ISTA Forest Tree and Shrub Seed Committee 2010 - 2011
Transcript of ISTA Forest Tree and Shrub Seed Committee 2010 - 2011
ISTAForest Tree and Shrub Seed
Committee2010 - 2013
ISTA Annual Meeting, Venlo NLJune 12, 2012
Chair Fabio Gorian Italy GER, BSC, RUL
Vice-chair Zdenka Prochazkova Czech Republic
Members Isolde D. KossmannFerraz
Brazil
Robert P. Karrfalt United States AOSA, MOI
Dave Kolotelo Canada
Stefanie Krämer Germany TEZ
Beti Piotto Italy
Hugh W. Pritchard United Kingdom STO
Heidi Røsok Bye Norway
Dale Simpson Canada
Victor Vankus United States AOSA
Rosa Colomer Spain
Efisio Mattana Italy
Czeslaw Koziol Poland
Vacant
FTSC MEMBERS
A- Rules developmentA1 Introduction of new methods
1.Water activity measurement
2.Alternative germination substrate for conservation programs and small lots
3.MOI: working sample
A- Rules developmentA1 Introduction of new methods
1. Alternative method to the moisture content determination2. MOI and AW were compared in 2012 in ITML 0600
Water activity measurement
A- Rules developmentA1 Introduction of new methods
A- Rules developmentA1 Introduction of new methods
A- Rules developmentA1 Introduction of new methods
The use of the agar for germination tests in conservation programs and with smalllots
1. sterile substrate;2. Homogeneous and constant moisture conditions
Proposal to Germination Committee and Storage
A- Rules developmentA1 Introduction of new methods
Discussion is open among MOI members to change ISTA rule n° 9.1.5.2 (and 9.1.5.5):
Proposal: also for large seeds (as tree seeds) 2 working samples each of 5 gr
MOI cutting working group
Scope 1: to verify if cutting is compulsory for the seeds of Pinus genus when the TSW is > 200 g.
Scope 2: to verify if it is possible to test 2 working samples of 5 g (or 10 g) of seeds instead of 5 seeds x 2 replicates.
1. ITML0600
2. CZDL0200
3. USDL0300
4. DEDL0400
5. FRDL0200
6. BEDL0200
Partecipating labs
Samples are in preparation for distribution
MOI cutting working group
A- Rules developmentA2 Introduction of new species
1.Validation of germination methods for tropical/subtropical species2.Tetrazolium 3.Pretreatments on Mediterranean shrub seeds4.Juglans spp.
Basic germination protocols for many tropical tree species are available.
Palm seed germination at 30°C
0
5
10
15
20
<20 <40 <60 <80
Mean time to germinate (d)
Num
ber o
f spe
cies
A- Rules developmentA2 Introduction of new species
34 Palm species (Pritchard, Mark, 2011)
Rubia peregrina subsp. peregrina Rubus ulmifolius
Species 1000 TSW TEZ test MOI behaviour
Rubia peregrina L. subsp. peregrina 14,5 g 95% Orthodox
Rubus ulmifolius Schott 2,6 g 93% Orthodox
ITML 0600
A- Rules developmentA2 Introduction of new species
A- Rules developmentA2 Introduction of new species
GERMINATION % OF SOME MEDITERRANEAN TREE AND SHRUBS SPECIES*(May 2012)
*Montaraz laboratories -Madrid (Spain)
SPECIES TREATMENT % GER DAYS
Arbutus unedo Soaking in water (48 hours) + Cold Stratificacion (1 month). Tª (15-20ºC) 65-80 28-35
Cistus clusii Bowling water (1 minute) 45-60 25-30
Cistus creticus Soaking in water (24 hours) 60-70 35-40
Cistus laurifolia Bowling water (5 seconds). Tª(15-20ºC) 40-50 25-30
Colutea arborescens Bowling water (5 seconds) + Soaking in water (24 hours). Tª(15/25ºC) 70-85 35-45
Cytisus multiflorus Bowling water (5 seconds). Tª(15) 80-90 28-35
Genista scorpius Bowling water (5 seconds) + Ice + Soaking in water (24 hours) . Tª(15-20ºC) 60-70 28-35
A- Rules developmentA2 Introduction of new species
GERMINATION % OF SOME MEDITERRANEAN TREE AND SHRUBS SPECIES*(May 2012)
*Montaraz laboratories -Madrid (Spain)
SPECIES TREATMENT % GER DAYS
Halimium atriplicifolium Bowling water (5 seconds). Tª(15-20ºC) 60-70 25-30
Pinus halepensis No soaking before sowing 85-95 14-21
Pinus nigra laricio No soaking before sowing 85-95 14-21
Pinus nigra salzmannii No soaking before sowing 85-95 14-21
Retama monosperma SA(3 h)+CA(24 h) 70-80 14-21
1. Both ISTA and AOSA no rules2. Important commercial species
Only among European laboratories
ViableNo viable
A- Rules developmentA2 Introduction of new species
Juglans spp.
B- PublicationsB1 Rules accompanying publications
1.Tree and shrub seed handbook (revision) No progress2.Sampling handbook (tree seed) Discussion is in progress
among BSC members about chapters 2,4,5 and 6
1.Guia de propagulos e plantulas da Amazonia, vol.2
2.Handbook on seedling evaluation(tree seed)
B- PublicationsB2 Training publications on specific seed testing topics
Fruits, Seeds and Seedlings of Amazonian Trees Vol. 2Ferraz, I.D.K., Camargo J.L.C. & Albuquerque M.C.F.
0123456789
10
Núm
ber o
f spe
cies
<10g 11-100g 101-1.000g
1.001-10.000g
10.001-100.000g
Thousand Seed Weight
0
1
2
3
4
5
Num
ber o
f spe
cies
0-1w 1-2w 2-3w 3-4w 1-2m 2-3m 3-4m 5-6m 7-8m 8-9m 9-10m 11-12m 1-2y
Mean Germination Time
Mean germination time: from 6 to 728 days
Seed size TSW varied between
3 g and 26,700 kg25 of 75 species concluded
25 species – Vol II
Species Fam. Species Fam
Duguetia chrysea Anno Bombacopsis nervosa Malv
Ephedranthus amazonicus Anno Carapa guianensis Meli
Xylopia amazonica Anno Carapa procera Meli
Couma guianensis Apoc Cedrela odorata Meli
Protium klugii Burs Brosimum parinarioides Mora
Protium peruvianum Burs Maquira sclerophyla Mora
Goupia glabra Cela Trymatococcus amazonicus Mora
Glycydendron amazonicum Euph Compsoneura ulei Myri
Abarema cochleata Faba Osteophloeum platyspermum Myri
Cedrelinga cataeniformis Faba Lacunaria jenmanii Quii
Eperua duckeana Faba Matayba arborescens Sapi
Eschweilera amazoniciformis Lecy
Eschweilera pseudodecolorans Lecy
Gustavia elliptica Lecy
Protium peruvianum Burseraceae
Osteophloeum platyspermum Myristicaceae
ISTA Annual Meeting, ZürichJune 14, 2011
1. Guia de propagulos e plantulas da Amazonia, vol.1 (English version)
2. Guia de propagulos e plantulas da Amazonia, vol.2
3. Handbook on seedling evaluation(tree seed)Ex. Adenocarpuscomplicatus
NormalAbnormal
The handbook “Seedlingevaluation” needs some standardization regarding the pictures…
1) Seed storage in polyethylene bags of a recalcitrant species (Quercus ilex): analysis of some bio-energetic and oxidative parametersS. Pasquini, M. Mizzau, E. Petrussa, E. Braidot, S. Patui, F. Gorian, M. Lambardi, A. Vianello
B- PublicationsB3 Scientific information publications
2) Interchangeable effects of giberellic acid and temperature on embryo growth, seed germination and epycotyl emergence in Ribes multiflorum ssp. sandalioticum(Grossulariaceae)E. Mattana, H.W. Pritchard, M. Porceddu, W.H. Stuppy, G. Bacchetta
B- PublicationsB3 Scientific information publications
1.Madrid (E): Workshop on Mediterranean species, June 20132.Milkow (PL): Workshop Current technologies of forest seed treatment, May 2012
C- Workshops and seminarsC1 Training and education workshops
Madrid Workshop, June 2013
Trees and shrubs seeds from the Mediterranean Basin: development of test methods to be
introduced into the ISTA Rules.
SEMILLAS MONTARAZ,
A SEED COMPANY WITH A QUALITY SEED LABORATORY
SPECIALIST OF TREES, SHRUBS, WILDS, HERBS AND NATIVE SEEDS SPECIES FROM THE MEDITERRANEAN AREA
SINCE 1990 – MADRID - SPAIN
QUALITY SEED LABORATORY
Skilled professionals
Personal member of TCM/FTS
Equipment and
protocols following
ISTA procedures
R&D on methods and treatments
Quality control, improvement, treatments and conservation of seeds from Mediterranean and Sub-tropical plant species.
850 Mediterranean and sub-tropical plant species of seeds from 1,800 plant species genotypes and varieties from 800 genera Mediterranean and worldwide.
Joint Research & Development programmes with universities and research centres on seed treatments and adaptation to environmental changes.
QUALITY SEED LABORATORY
Plant Biology DepartmentEscuela Universitaria de Ingeniería Técnica Agrícola
Technical University of Madrid• Located near de city center with good public transport (underground)• Conference room and laboratories
Researchlaboratory
Teachinglaboratory
Seminar room
The team
• Seed germination and conservation research group:– Félix Pérez-García (Professor)– M. Elena González-Benito (Professor)– Sara Mira (lecturer)
• Over 25 years experience on seed germination and conservation research work mainly with Mediterranean species
Seed germination of Phillyrea angustifolia
• Phillyrea angustifolia
• Oleaceae, related to Ligustrum and Olea
• A representative evergreen Mediterranean shrub
• Distribution: Northern Africa and South of Europe
• Economic importance: ornamental
• Fruit: black-blue drupe with lignified endocarp
• Seed oil content: 21%
•
Mira S., Ruiz C., González-Benito M.E. and Pérez-García F.
Departamento de Biología Vegetal, E.U.I.T. Agrícola, Universidad Politécnica de Madrid (UPM),Madrid, Spain
CONCLUSIONS:
• Germination needs the complete removal of the endocarp
• Germination is higher at 15-20ºC
• Seed germinations is faster at 15ºC
• Water imbibition for 72h increases the speed of germination
• Cold stratification has a negative effect on seed germination
• Plant growth in greenhouse is very slow
Seed germination of Phillyrea angustifolia
WORKSHOPCURRENT TEHNOLOGY OF FOREST SEED TREATMENT
21-25 MAY 2012, MILKOW, POLANDKostrzyca Forest Genebank
WORKSHOPMiłków, Poland
The aims of the workshop were to provide information on:
- the use of Polish forestry practices and treatment of seed material, - dissemination of actions, to encourage workshop participants to include set standards for work procedures used in their business units,- creating the possibility of an international exchange of experience
The workshop participants came from 11 European countries and they represented
the following institutions: State Forests
National ParksUniversities and Research Institutes
ISTAENSCONETMSB Kew
FAO
WORKSHOPMiłków, Poland
The main goals of the workshop
1. Report from the FAO Forest Genetic Resources Working Group - the status of forests / forest conservation in Europe.
2. Collection of seeds of forest trees and shrubs, herbaceous plants and crops - a comparison of accepted standards.
3. Seed quality testing and methods of storage:storage methods (length and conditions) depending on the category of seeds (orthodox, suborthodox, recalcitrant).Creation of seed samples.
4. Stratification and other dormancy breaking methods: stratification in the substrate and without substrate; length and conditions of stratification; other dormancy breaking methods
WORKSHOPMiłków, Poland
The main conclusions of the workshop
1.Workshop participants exchanged their knowledge and their experience in topics related to collection, evaluation and storage of herbaceous plants, crops and forest seeds. 2.Emphasized the need to organize the next workshop in another seed bank.3.Emphasized the necessity of extend educational activities for private forest owners relating to the purchase, handling and planting of recommended forest reproductive material in the context of ongoing climate changes
WORKSHOPMiłków, Poland
Paris, April 24-25, 2012OECD-Technical Working Group on forest scheme
1.Guidelines on the FRM chain2.Short rotation plantation for bioenergy and agro-forestry and shrub species3.Proposal to introduce the category “Tested” in the foreste scheme4.Uganda new member (26th)
Thank you for your attention!