Disclosure to Promote the Right To Information

Whereas the Parliament of India has set out to provide a practical regime of right to information for citizens to secure access to information under the control of public authorities, in order to promote transparency and accountability in the working of every public authority, and whereas the attached publication of the Bureau of Indian Standards is of particular interest to the public, particularly disadvantaged communities and those engaged in the pursuit of education and knowledge, the attached public safety standard is made available to promote the timely dissemination of this information in an accurate manner to the public.

इंटरनेट मानक

“!ान $ एक न' भारत का +नम-ण”Satyanarayan Gangaram Pitroda

“Invent a New India Using Knowledge”

“प0रा1 को छोड न' 5 तरफ”Jawaharlal Nehru

“Step Out From the Old to the New”

“जान1 का अ+धकार, जी1 का अ+धकार”Mazdoor Kisan Shakti Sangathan

“The Right to Information, The Right to Live”

“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता है”Bhartṛhari—Nītiśatakam

“Knowledge is such a treasure which cannot be stolen”

“Invent a New India Using Knowledge”

है”ह”ह

IS 873 (1974): Liquid Glucose [FAD 2: Sugar Industry]

Indian StandardSPECIFICATION FOR

LIQUID GLUCOSE

( First Revision)

Fourth Reprint JANUARY 2005( Incorporating Amendment No. Iand Including Amendment No.2)

UDC 664.162-404

IS : 873 • 1874REAFFIRMED

2005

Gr6

C Copyright1983

BUREAU OF INDIAN STANDARDSMANAK BHAVAN. 9 BAHADURSHAHZAFARMARG

NEW DELHI 110002

October 1974

xxx2010

AMENDMENT NO. 3 JUNE 2011 TO

IS 873 : 1974 SPECIFICATION FOR LIQUID GLUCOSE

(First Revision)

[Page 6, clause 5.2(f)] — Substitute ‘Net quantity’ for ‘Net mass’. [Page 6, clause 5.2(h) (see also Amendment No. 2)] — Substitute the following for the existing:

‘ Any other requirements as given under the Standards of Weights and Measures (Packaged Commodities) Rules, 1977 and the Prevention of Food Adulteration Act, 1955 and the Rules framed thereunder.’

[Page 6, clause 5.2.1, line 9] — Substitute ‘Net quantity’ for ‘Net mass’. (FAD 2)

Reprography Unit, BIS, New Delhi, India

Indian StandardSPECIFICATION FOR

LIQUID GLUCOSB

(First Revision JEdible Starches and Glucose Sectional Committee, AFDC 33

.",.,."",Directorate General or Health Servicea ( MiDlatry of

Health It FamilyPlumial ), New Delbl

M'-'"DB S. Bo.. Natioaal Supr lnatitute ( MInistry 0' ApicWture )

( Department 01PoocI), ICaDpurDB A. N. SalVAftAYA ( MIawII)

SIUQ D. S. CHADHA OeDtral Committee lor Food StaDdarda (~01Health. Family PIaDDiDI ).. New DeIhl

Sa n..1 Mt1KBDJD (MImM,.)SDI V. P. CIIADJIA Bbarat Starcb & Chemicals Limited. New DeIbi

SIIBI N. n, S'.OBAL ( AI"",." )CJWaIU. Technical StandardiaatioD Committee (YIaIatIy 01

A,ricuIture ). NewDeihlSJIOaftABY ( AI",." )

SJIJU CBDt7BB.. J MAlO....1 AU India Starch Manwaeturen AIIociatioa,· Bombay

SUI B. K. SABDAKA (~I,,",,*)SIDU H. Il. S. IYDGAB IDcIJaD CoDreetloDery MaauCaeturera' AMociadoa,

New Delhi8DJ L. N. MA'IB1JB (~ )

SOl .R.. S. Ina lacIJaD Chemical Manufacturers' ~_Caleutta

SBBJ O. K. NAJa( ~,.." )SJIaIll. V. JVXAUAB ADD Starch Product. Ltd, Ahmed.bad

SOlO. N. PA.IEB ~ MIIrMII )DB 0. R..KaJ.....oo.HY Tapioca Market EqluaiOD Board,~ 01

KeraIa, TriftDdrumSUI s.K.. L~K'BJ01IdA~A.A CeDtral'ood TecbDoJocical RaearcIl Iaad,,*c.;::: ). Myae

Da H. S. R. Da,UOBA. (~SUI S. K. MnD.,_ ..... Supr r... Ltd, Ravalpoa

Da R. S. Dv.JIY (~ )( e",,;.,w • ,.,. 2 )

.. Copyright J983

BUREAU OF INDIAN STANDARDS". publica" II ...... UDder ",..~ .. ( XIV .f lIS') ...~~.IDwboll••~ '" My ...,' Wlda wriU::;-_." tIM.......... be...... to be __t or ."...... die ... AcI.

JI.I7I •.-

("""'~Jr-"" I )

M.IIn ".",..,

SJmI A. S. IluADIIYAUB Ctom ProductaCo ( Inelia ) Private Limited, BombaySimi S. ll. PALRAB ( MImwU )

SOl v.IluEVlld . Kamala Supr MilIa Ltd, UdamalpetSOl N. N. K. Ph.AD ( M....,. )

SHBI S.IUIU.WAIIY DIrectorate Geperal or Techaical Development,New Delhi

8..1 S. N. lUo Du • Company, BombayS-m M. N. HAD ( M....,. )

SBIU M. G. SA'nIJI Pederatlon orBilcuit Manufacturen or India. DelhiS"IR.D.~WAua(~... )

0. P. M. SIUB Maize ProclUetl, Ahmedabad8..1 V. C. PAUL ( AI...." )

CoL K. N. SS••JU. ~eraauterGeneral'. Branch, Army Headquarters,New Delhi

L-r-CoL O. P. K.~ ( ~;t""'" )DBHABI Buow._, DirectorGaleral, lSI ( &-qffiM MIffIb,,)

Director ( AlI'i. Food )

s.r.-;,SD! G. S. VILKJIV

Deputy Director ( Api • Food ). lSI

2

AMENDMENT NO. 2 NOVEMBER 1995TO

IS 873: 1974 SPECIFICATION FOR LIQUID GLUCOSE( Fint Revision)

( Page 3, clause 0.1, last sentence ) - Substitute the following for tbeexisting:

'It may be manufactured by acid or enzyme hydrolysis of stllCh. The source ofstarcb materials generaOy used include tapioca, corn and rice.'

( Page 3, claus« O.s, line 3 ) - Add 'and the Standards of Weights andMeasures ( Pac1ctlged Commodities) Rules,1977' after the word 'thereunder'.

( Page 4, clause Z.I, line 4 ) - Add 'acid er enzyme' before tbe wold'hydrolysis'•

(Page 4, clause 4.1, line2 ) - Substitute the following for the existing:

'It shall be clear, free from Cennentation, mouldy growth, sediment, dirt or othersuspended and extraneous matter, or added sweetening or flavouring agents orany other deleterious substances.'

(Page S, Table 1) - Substitute the following for the existing:

M811IOD OFTEsT,REP 10

(4)

AD_x C of Ibis lII.clard

5 otIS 4706 (Part 2): 1978­

13 of IS 4706 (Part 2 ) : 1978­

I10f IS 4706 ( Plrt 2) : 1978-

13of IS 6287 : 1985t

15of IS6287 : 1985t

14 of IS 6287: 1985t

0.3

4.8to 5.5

40400( For

lupr coDfectioDery )

1

5

2

(2)

TABLE 1 REQUIREMENTS FOR UQUID GLUCOSE (ALL GRADD)(Clause 4.4)

REounu!MENT

(3)

80TotallOlicIs, percentby maa.Mu.

Ash, pereentbyIIWI, Jla

iii) pH

iv) Sulphurcloxide,mlftl, JltIJt

v) Ane.ie, mAt MtIJl

vi) Copper. "JIkI.Ma

vii) Lead, mAtMa

(1)

i)

ii)

SLNo. CJwlAcrEIusnc

1

Amend No.1 to IS 873 : 1'74

TABLE 1 ( Coaduclecl)SL No. CIlARACl'KRIS'nC REQUIREMENT MITHODO.TIsT,RI.1'0

(Q (~ Q) ~)

viii) EscherdtitJ Coli, in 20 I Absellt IS 5887( Pan 1) : 1976*

ix) SlllmOMI"'. in 20 I AbseIl1 IS 5887( Part 3) : 19761

-Methodsof tesl foredibleslarchMaDd starchproducts: Part2 Cbemi<21 methods(first revisio" ).

fMelhodsofsampliDIaDd analysis for suaarconfectioDery (pnt revisio,,).

*Methodsof detectioD of bacteriaresponsible forfoodpoilODiaa: Part llsolatioD, identificatioD andeDumeration of&e1NrdtitJ Coli (fin' IWisioII ).

f Methods of detectionof bacteriaJeSpouibie forfoodpoilOaiaa : Part3 IlOlAtioD aDd idealificatioDof S,I1Ift011eIl. aDd $JUgel'" (fint revisio,,).

(Page 6, clause 5.2(c) ] - Substitutethe followingfor the existing:

'c) Nameand addJess of manufacturer,'.

( Page6, clause 5.1 ) - Add the followingIt the end:

'g) Dateof expiry;

b) Any other details required under the Stdndards 01 Weights andMeasures ( Packaged Commodities) Rules, 1977.'

( Page6, clause 5.1.2 ) - Substitutethe followingfor the existing:

'5.3 DIS Certification Marking

The productmayalso be marked with the SgDd.rd Mark.

NOlB - n. use of lb. Standard M.rk is aowmed by the provisioDi of abeBureau ofIDdian Sian." Act, 1986aDd the Rules aDd Rep1aliou made thereuDeler. 11le .lails ofconditions uDder which • licence for tbe use of Stadard Mark may be lranced tomanufacturers or proce.oll maybe obtaiDeeI fro.....Bureau of IDdia.StaDdarcls.·

( Page6, clause 7.2, line 2 ) - Substitute 'IS 107$) : 1992' lor 'IS : 1070 •1960'.

( Page 6, foot-note with c.' ,,",rk ) - Substitute the following for theexisting tide:

•-ReapDI pde wlter( dUrtl rMsitM ).'

( Page 7, clause A.2.1, line 2 ) - Delete the words cof the RegularConversion grade material' .

(FAD2)

Printed at N.. lDclia Prifttina Pr_, Khurja, Indi.

2

18 1173 • 117t

Indian StandardSPECIFICATION FOR

LIQUID GLUCOSE

( First Revision)o. FOREWORD

0.1 This Indian Standard (First Revision) was adopted by the IndianStandards Institution on 11 March 1974, after the draft finalized by theEdible Starches and Glucose Sectional Committee had been approved bythe Agricultural and Food Products Division Council.

0.2 Liquid glucose is extensively used in confectionery, biscuit and foodcanning industries. To a limited extent, it also finds use in textile, leatherand paper industries. It is, at present, manufactured in the country bythe Cacid hydrolysis' of starch.

0.3 This standard was first published in 1956. The various flovisions ofthat standard had been under the review of the Sectiona Committeeresponsible for the preparation of this standard, and the present revisionbased on the experience gained both by manufacturer s and users, is beingissued. This revision incorporates a number of important modifications,namely, (a) number of grades has been increased from three to five incor­porating additional grades of regi..dar and extra high conversions; (b) therequirements of total solids, sulphated ash, iron, zinc and tin have beendeleted; (c) the additional requirements of total solids, dextrose equivalent(DE:' value have been included; and (d) the limit for ash has beenlowered.

0.4 Thia stendard stipulates the use ofa 2·54-em cell in Lovibond Tintometcrfor determining the colour of liquid glucose. While realizing thatmeasuring colour in lO-cm and 15-cm cells would make colourdetermination more accurate and precise, the Sectional Committee, forwant of data, decided not to make any change in the Indian Standard atthis juncture. However, the Committee recommended that comparativedata using different sues of cells in determining colour of liquid glucoseshould be collected, and if in the light of these results it was foundnecessary, the Indian Standard should be modified.

0.5 In the P' eparation of this standard due consideration has been givento the Prevention of Food Adulteration Act, 1954 and the Rules framedthereunder. However, this standard is subject to the restrictions impos~~

under these, wherever applicable.

3

111175 • 1174

0.1 For the purpose ordeci~ whether a particular r~emeDt ofthilstandard is complied with, ,the fiDal value, obaerved or calculated, exprea­ing the result of a teat or analysis, ahall be rounded off in accordance withIS : 2·1960·. The number of lignificant places retaiDed in the rounded offvalue should be the same as that of the specified value in thia 1taDdard.

1. SCOPB

1.1 Thi. standard paescribea the requirements and the methods ofsamplingand teat Cor liquid glucose.

2- TERMINOLOGY

2.1 For the purpose of this Itandard. liquid glucose or glucose syrup .haDmean a refined and concentrated non-erystalJizable aCJueoul solution ord-glucose, maltose and other polymen of d-glucolc, obtamed by controDedhydrolysis of starch containing material.

S. GRADEl

3.1 The material shall be of the following five lI'ad~:

a) Low convenion (LC),b) Regular conversion (RC),c) Intermediate conversion ( Ie J,d) High conversion ~ He), ande) Extra high conversion ( EHe).

4. IlEQ,UIRBMBNTI

4.1 The material shall be in the form of an odourleaa and vilcou» ayrupwith a characteristic sweet taste. It shall be clear, tree trom rermen­tatioD; mouldy growth, sediment, dirt or other IUlpended and extraneoulmatter, or added sweetening and flavouring agentle

t.2 Colo... - When a 50 percent (_/,,) solution oftbe material of regularconveraiongrade is tested in a Lovibond Tintometer in a 2'M-em cell bythe method prescribed in Appendix A, the colour of the material in termsof Lovibond units shall not be deeper than:

a) 0-1 yellow and 0·1 red within a period of 90 daya from the dateof manufacture, and

b) O~ yellow and 0'1 red within a period of ISO days from the dateof manufacture.

-aulel lor round.. offnumericalvalues ( "",.).

4

111173 ·lI7t

U Whealdeten;Dined accordin, to the method prescribed inAp~ B,the datrole equivalent (DE) value for difFerent graclea ahaIl be asfollOWl:

Low CODVcrUOD ( LC ) 28 to 37R.egular conversion (RC) 38 to 47Intermediate conversion ( LC ) 48 to 57High conversion ( HC ) 38 to 67Extra high CODvenion (EHC ) 68 and abovr

t.t The metarial shall also comply with the requirements given inTabJe 1.

TAIILB 1 RBQ,tJlRDlBNTS .OR UQ.UID GLOCOSE ( ALL GRADEl )

8L ~U'ftalftlO 1lIIQ'OIIUIIDft MB'BOD 01' T... 1lIII' -.roNo. r- ,J..

Appendix QNo.o(IS : 4706-1968-

( I) (2) (S) (4) (5)

i) TotaiIOUda,perceat by 80 C.... Mia

II) Alb.. perceDt by ...., 0-1 ..Ma

Iii) ,H 4-8 to 5·5 10iY) Sulphur diaDde. ppm, 400 8

Alav) Aneaic, ppm, Ma 1-0 D

vi) Copper, ppm, Ma 5 BYIl) Lead, ppm. Ma 2 p

·Metboda or tat for edible stuchel.

5. PACKING AND MARKING

5.1 PacIdDl- The material ahall be packed in dry and leak-proofcontainen. Unless otherwise a~ between the purchaser and thevendor. such containen may be either lacquered or lined steel druma, ormade of tin plats.

5.2 MarklDI - The container. shall be suitably marked witl- theroUowing information:

4&) Liquid glucose.b) Grade of the material.

II-m • 1174

c) Name of the manufacturer,d) Date of manufacture,e) Batch or code number, aDdf) Net mass of the contents.

5.2.1 The words ·liquid glucole' ( ,,, 5.2 <a)] .hall be marked insufficiently bold letters as compared with other paniculan:

&""'1":LlQ,UID GLUCOa

Intermediate ConvenionXYZ &. Co20 Mar 1974Batch No. 593Net mass-25 kg

5.2.2 The product may also be marked with Standard mark.

5.2.', The use of tbe Standard Mark is governed by the provisions of theBureau a/Indian Standards Act, 1986 and the Rules and Regulations madethereunder, The details of conditions under whicb tbe licence Cor tbe use ofStandard Mark may be gnntcd to manufacturers or producers may be obtained(rom tbe Bureau of Indian Standards.

I. SAMPLING

6.1 The method of drawing representative samples of the material andthe criteria for conformity shall be as given in Appeadix G.

7. TBIT8

7.1 The testa .hall be carried out as prescribed in 4.2, U and col 4aDd 5 orTable J.

74 Q,tIaue, of~ - Unless specified otherwiae, pure chemicaJaaDd cliatiDccI water (_IS: 1070-1960· ) Ihall be employed in tat..

Non _.... ....Iea..• ...1 mean chemica" that do DOt coatalD lmpurI".......1MftIUItI.,...,.·SpeciftcatioDW. ~tft'........ qaaIity ( ,,.,,.).

6

11,171. __

APPBNDIX A( Claar, 4.2 )

DB~A'I10N or COLOn

A..e. GBNBUL

&.1.1 This method determin. the colour or a 50 percent ( -/.) IOlatioaof the material in water, by comparison with Lovibond 1Iaae. oIbowacolour characteriaticl_

.&-1. APPAllATUI

A-l.11.nIIaa..t 11ateaaeter

A-l.2 GIaM Cell - 2-54-cm, made of clear, coIouriea glau.

U DOCBDURB

Ul Pnpuad.. of .. 8oIad.. - Prepare a 50 percIIlt ( -/, )dation or the R.egular Convenion grade material in water.

A-2.2 Clean the 2·M-cm glas. cell with carbon tetrachloride to removeany oily or arearY film on it, and allow it to dry. Fill the cellwith the~pared101ution ('11 A-2.1) and place it in position in the Tmtometcr.Place alODl aide or it IUch red aiidJor yellow Lovibond ,~ u areD_ry to match the colour shade of the prepared 101utioo. obIerviIwthecoloun or the prepared lOlution and or the combiDation or LoviboDaglula through the eye-piece.

A-& IlBPOIlT

W.I Report the colour or the preplred IOlution (COD~~t-/_ of the material) in terms of Lovibond UDiti by upiDdiviclually the value. for the red and yellow LoviboDd gta- • :

Colour~ of a 50 percent(-/_) lO1utioD of the materialill water. iD 2·M-em cell OIlthe LovibaDcllCa1e - .Y + 6R

where• - the ... total or the various yellow (Y) Loviboad ___..... \

6 - the .. total 01 the veiOUI red (1l) LoviboDd at-­UMd.

7

A.PPBNDIX B( C14us, 4.3 )

_ATlON or DBXTIlOIB BQ,UIVALENT VALUB

-'1. JllUNCIPLB

8-1.1 Titration of the sugar solution with Fehling'. solution at boilingpoint UIing methylene blue .. indicator. The voluma of the solutionsare acijuated in Ncb a way that the total volume at the end orthe titratioDhal • Biven value ( 75 :I: 5 ml ).

-.2. UAGBN'n

-.a.1 'eWbaI'- 801...Solution A - Aqueous IOlutiOD or copper sulphate containiDg 69-28 I

or CuSO" 58.0 per litre.Solution B- Aqueoua IOlution containing 346 I of lOdium ~um

tartrate, potallium tetrahycltate aDd 100 g of lOdiumhydroxide per litre.

NoD-,.. the mixed lOIutioal are Ullltable Ja die praeace or air.1DiDDI II claDe........ 1... .MI.I CtIlUw-.. t/'"PIIalia(1 SoltdiOfI- Titrate the Fehlinl'llolution

tbua~ with the ItaDdarcl101utioD ofdextrole ( 11-2.2 ) .. indicatedlD MI. Let V, be the DUlDber of milli1itrel of deztroae IOlution uaed;tbiI value ahould be equal to 40':1: 0·5 mi. Otherwise, the FebIiDI'lIOhatioD ahould be adjusted.

114.2 laleelercl 1oI11t1oa 01 Baa..- Diaolve 2-5 I of the ewedauoee dried beforelwul at 70°0 UDder reduced preuure, in diat1I1edwater to make 1000 mL

a.u II8dIJI-e III..WI..- - ODe perceat aqueoUi lolutioD.

Nt AWAaATUI

N.1 0nliDary laboratory equipmet.

NeLl V__"""" - 500 mLMI.2 .".M.t Pilfllll .... 10mL

MI.I..."B'"NeIA CAn....",

N.I~COlI'" 01 tbelallGWiDl parIL

I

111171 • 1174

II-U.I CMiI., FW .IA II N.,.,. Moali- 300 mi.

a&2.2 B,.",. IIIiIA SItIHIt ", S. B.",,, ... 50 mlgraduated in 0·1 mIaDdlUitable protection plate.

N.2.3 .A H••, DIIIiu -- which we>uld .ure bo~ under thecoaditioDI indicated in B.f and which would enable lightiDg. to determinethe ead point without baviDg to move the conical fluk.

MI T.. ~-eIea- Weil~ to the nearest 0·001 g a quantity or IUDplein IUch a way that Uter dilution to 500 mi. the test lolution containlapproximately 0·25 I or .-educing 'ucan expressed al dextrose for 100 mi.(This teat portion generally weighs between 1 and 10 g. )

a.t.2 Det.....-d.. - With the help oi the pipette, transfer 10ml eachor IOluUoDI A and B or the Fehling'. solution into the 300-ml conicalfIuk. Add. boiling replatOf, that ii, pumice atone or glaa marblClaDd enough of water to raise the total volume of the liquid (Fehling'.IOlution + water + tat solution) to 75 ml :I: 5 ml at the end of thetitration. In order to determine the quantity of water that is added, it is0_ neceaary to eany out a preliminary determination using the quantityofwater. considered efFective for covering evaporation, for inltaace 40 mi.ht the conical ftuk on the heating device. Ilisht from the ltart of'-tiDI. pour with the help of the burette the sugar lO1utioD amountingwitbia 0-5 m1 of the anticipated eDd point (determined by • preliminarytell in which the tat solution is pdually added tiD the end. point isreached)_ Adjult the heating in IUch a way as to make the 101ution boilwithin 2-75 • 0-25 min and then make DO more adjustment till the eDdof the tCit. The boiliDl should be brisk and coDtiDuoUi all alODI the~tioD otherwise air would enter the fIuk and oxidize itl cooteat..It is therefore euential that the flask it DOt lhaken after the heatinS atartI.After 2 minuta of boiliDI. add 2 drops of methylene blue lOIution aDdcomplete the tittation drop by drop. At the approach of the end point,obIeIYe • time of teD to Been teeODcIa between the addition of twoIUCClllive _dro~. Carry oat the operation till the blue colour vaaiaheLTItradoD. Ihou1cI be comPleted withia 1-' to 21» min after the aclditioD 01the iDdicator.

Ml.1 Le& VI be the Damberor mDlDitres or tat IOlution UIed. If this901- 11110I 40 • 5 ml. it II MeIII.ry to moctiry the CODCefttratioD of thetilt ICIIudoa. It II reawnlllf:Dded to check at the ad of the titration if the1Dal.a-1WII witbia the prelCribod limits.Carry out two dctermiDatiolll-_....mpIe.

•t

II a171 • 1174

11-5. BXnBIIION or BBlULD

11-5.1 The reducing IUpr expreued u dextrose, percent by mau, isequal to:

2-5 see 100 125 V,iOOO x V, x y;- X E - Yi X E

whereE - mau, in g, of the telt portion;

V. - volume, in ml, or the standard dextrose solution (8-2.2)used for the calibration of the Fehling'. 101ution; and .

VI .. volume, in mit of the test solution required for reducins20 ml of the Fehling'. IOlution.

APPENDIX C[Tal, 1. 111m (i) ]

DlC1DIIINA110N 01 TOTAL SOLIDI

e-l. BQ,UlPMENT AND APPARATUI

c.l~ The foUowinl equipment and apparatUi are required.

04.1 Dlatomaceo_ BanIa - Neutral to Utmul when moilteDed .withwater. [ If. commercial grade iI used, wash it by percolation with waterwhich baa been slightly acidulated with hydrochloric acid, until the eftIuentis acidic to lltmUi. Then rewuh with water (not acidulated), UDlll theeftluent is DeUtral to litmul. Finally dry the earth in an oven atabout 10500. Jc.l.2... Idrft.l RocI - approximately 6O-mm 10Dl with aflattened end.

C-l.3 Mol.a... Dl8Ia - with a cover made of aluminium; approximately25 mm in h~ilht and 75 mm in diameter.

C.l.t Desiccator - containq pholphorua pentoxide .. desiccant.

Q.l.5 NicILelleeap

o.J.6 \'aca•• o..a10

C4. PaOCBDlJU

e-2.1 Place about 10 I of the diatomaceoua earth aDd the llul ltirriDgrod in the moisture dish aDd dry the -diatomaceoUi earth ill an air OVeD atabout 105·C. Cool in the daiccator aDd wash. R.epeat dryiq in, theair oven. cooling in the desiccator and weighing till the mas. I. CoDltaDt.Note the combined mall of the cliIbwith the cover, the II.. ItirriDI rodand the dry diatomaceous earth. Wash accurately about .5 I or thematerial in the nicke1lCOOp, miK it with .5 mI of water and run 011 to thediatomaceoUI earth, uaing the glall stirring rod. Wash the scoop thrice,using 2 m1 of water for each washing and tran.fer the washiDp on todiatomaceous earth. limultaneously workiDI the contents of the moisturedish into a thick paate uainc the llua ltirr!q rod. Leave the 1_stirring rod in the diaL Place the moisture dish in the vacuum oven anddry the contents or dish at 100°0. Cool the dilh and its conteotl in thedesiccator and wash. llepeat dryinl in the vacuum oven cooliDs in thedaiccator and weiahiDI till the mall is CODitant.

e-s. CAl..CULATiON

TotailOUcb coatent, [ 100 ( AI + ~ - B1 ]percent by IDUI - 100 - AI

where],I - IDUI in I of the material taken ror the tat;~ - IIWI ill I of the moisture cliah containing the IIua stirr­

ing rod and dried diatomacooua earth; aDdB - mall in I of the lDOilture dish COD~ the sJ"

It~ rod, diatomaceous earth and the dehydr4lteclmaterial.

APPENDIX D[Tall 1,1_ (v) )

DBIaIIINATION 01' AIlIBNIC

0.1. AWAIlA'I11I

1).1.0 The followiD, apparatUi aacmbled u ihown in Fil. I is required.

0.1.1 WWelia dt-..-12OmL

11

II•• ·117.-D-l.2 ...T_- made from ordinary gIaa tubiDl, aDd bavinla totalleDp or 200 DUD. It bu an iatcmal diameter of cuetly 6-5IDDl aDda.aterDal diameter of about 8 DUD. It it drawn out at one eDd to adiameter of about ODe mDlimetre and a hole DOt Iell thaD 2 mID indiameter II blown iA the aide of the tube, Dear the COllltricted pan. DIeupper ead of the tube is cut off.quare aDd iI either IO'&Ddcd off llichdy« FOundamooth.

••I

_Ifl"- ... OIA ~J

...-.....

_....". .....or ••I..."

.......-FaD. 1 ,A_eLY'owAw~ WIa

. DIrnaImrA'rDI • eA ....

II

II a171 • 1174

D-l.J IlaWIer 11_. - three. One fitl exactly into the mouth of thewide mouth bottle and has a hole bored centraUy to take the tube fromits conatricted end. Each of the other two rubber bunp (about25 X 25mm) hal a hole, exactly 6-5 mm in diameter, bored centraDyand is fitted with a rubber band or spring clip for holding them tichtlytogether_

1).1.4 P........tloa 0' the Ola•• Tabe - Moisten a Imall quantity ofcotton wool with lead acetate solution (s" 0-2.1) and then dry it in •dUlt-free atmosphere, Lightly pack the glau tube with this cotton wool.10 that the upper surface of the cotton wool is not leiS than 25 nun belowthe top of the tube. Insert the upper end of the tube into the nurowend,of one of the pair of rubber bungs (about 25 X 25 mm lize) eitherto a depth of about 10 mm ( when the tube hal a rounded oft' end) or 10that· the ground end of the tube is flush with the larger end of the bUIll.Place a piece of mercuric chloride paper (s" D-2.2 ) flat on the top of thebung. Place the other bung over this with itl larger end in contact withthe piece of mercun-e chloride paper. Falten the two bungs by meansof the rubber band or the spring clip, in IUcb a manner that the bom,.of the two bunp ( or the upper bung and the glalS tube) meet to form •true tube of 6·5 mm diameter interrupted by • diaphragm of mercuricchloride paper.

1).1.4.1 Initead of this method of attaching the mercuric chloride paj)Cr,any other method may be used, provided. that: (a) the whole ortheevolvedpi pusa through the paper; (b) the portion of the paper incontact with the pi is a circle of 6·5 mm diameter. and (c) the paper iIprotected from IUDliSht duril1l the test,

0.2. ItBAGBNft

D-U The Collowinl reagentl arc required. (The reagenta with theaception of 0.2.10 aDd D-2.11 abal1 be free from traeea of aneaic. )

D-2.1 LeU Acetate Wad.D -10-0 percent (III''') ill water, reeendyboiled and cooled.

D-U Mwcnarle aaa-Ici. Papa- - smooth white filter paper. Dot leuthan 25 mm in width, lOakecl in a laturated lolution of mercuric chloridein water;~ed to remove lu~rftuoUi lolution. and dried at about &ereill the dark. The pUe of the filter paper .haD be iucb that the lUll ill!:i~ Ibal1 be betWeea 65 aDd 120; the thickncu in mID of 400 papen

be .p~tely equal, numerically, to the IDUI in IIml • Mercuricchloride 'p.per~ be ltored in a .toppered botde in die clark. Pa~which havebeen Gpoled to aunUght or to the vapour or ammODia abOuIdDOt be UIecl u they pve ~ter Colourccl .taiDI or DO .taIa at ·aII wbeDemployed iD the quuatitadYe tat ror aneaic.

IS

111171·U7t

D-U aw.......I.... - Acld to a amaU quantity of water !O I orpotaaium bromide. DiIIoIve the potaIIium bromide in the water ADdmix with it 30 I of bromine. Doute with water to produce JOO mla.mi&.

D-U eoa.....tecl Byclroaa.Ic AcltI- sp If 1-16 and complyiDgwith the (oUowinc tat:

Dilute 10 m1 of the concentrated hydrochloric acid with sufficientwater to produce 50 ml, add 5 m1 of ammonium thiocy~te aoIution[ 10 percent ( JIll' ) in water] and .tir immediately_ No colour shallbe produced.

1).2.5 ......teII HfClreclaloric AelcI- Mix together one millilitre of6romine solution aDd 100 m1 of concentrated hydrochloric acid.

D-2.1 ltaDaoa CIaIorIde SoIad. - Dilute 60 mI or concentratedhydrochloric acid with 20 m1 of water, add to it 20 I of tin, heat geDtlyuDtiJ gas ceuel to evolve, and add sufficient water to produce JOO ml,aUowiDS the undiuolved tin to remain in the solution. Decant the clear.oIution. add aD equal volume of concentrated hydrochloric acid, boilclown to the origiDal volume and filter through a fine-grained filter paper.

B-2.7 P....... locIId. - crystals or powder.

D-U Zlac - granulated and complying with the following test:

Take 50 m1 of water. 10 mJ of stannated hydrochloric acid( ,. D4.I ) and 0-1 ml of dilute solution of arlCDic (I" D-2.11 ) inthe wide mouth bottle. Add one gram of potassium iodide and 10 Iof zinc. ~ place the prepared glau tube (I. 1).1.4)' inposition. Allow the reaction to continue for one hour. A faiDibut diatiDet yellow .tain thall be producod on the merewicchloride paPfL--

D-U Iea...tell B,uocJa1orle Aeld - Mix together one millilitre of1taDD0UI chloride lO1utiOll and 100 mJ of concentrated hyclroch1oric ae~.

D4.10 IU-I 80JadMa ., Anealc - DiIIoIve 0-132 I of arsenictrioxide in 50 ml or CODCeDtrated hydrochloric acid, 8dcl lufBcient waterto produce 100ml and mix.

B-UI DIhd. W1dI_ e6 AneJIIc - frahly prepared. Dilute ODemUliUtre olltrODl lO1utioD of anenic with water aUlident to produceJOO mi. ThiI dutioD coataiDI (1)J JDI 01 aneaUc (or 0-013 2 DI, orMAl perml.

D-S. BCKDtJDD4al~et. W."-W~~10loltbemat.w, cO.oIve it Ia 50 mI orwater,.. acid to It 10 iII-oIl1ittt1,IM ted

14

IItl7l.11H

hyclrochloric aciel. AUow the IOhatioD to ItaDd for five minutea aaclremove the excea of bromine with • few drops or stannous chloridelO1ution.

D-I.2 Placethe whole of the prepared IOlutioD (1M 1).3.1) in the widemouth botde and add 04e gram of potulium iodide and 109 of aiDe.Qpickly place the prepared gJaIl tube ( III D-I.t) in ~itiOl1. Allow thereaction to continue (or 40 minut. ( III.u. Note under D-3.2.3). Removethe piece of mercuric chloride paper at the end of this period. Compare theyellow stain, produced on the mercuric chloride paper if arsenic i.praent in the material, by day light with the standard ,tain prepared uilacnDed under D-U.

o.UeI The limit prescribed in Table 1 shall be taken as not havialbeen exceeded if the Itain produced by operating the material is Dotdeeper thaD the standard Itain ( ,,, B-3.3 ).

D-S.2.2 ComJNltisOfi .f SItJias - The compariaon of the stains is madewith freshly prepared atain immediately at the completion of the tat.

D-S.2.S A blank determination shall be carried out under the sameeonditions, on the same reagents [used both in the preparation of thesolution ( '" 1).3.1 ) and in the test ( s. D-S.2)] and by the same perIODbut without using the material. The blank ahalI Dot produce anyvisible ltain on the mercuric chloride paper.

HOI'JI - The reactioD may be accelerated by p~cj.1 the apparatul QD • wanDaurI.ce. care beiDi takeD that the mercuric chloride paper mDaIDa quite drytiLroupout the test. The IDOIt luitable temperature lor carryiDa out the teetIIpaeraJly_ about we. but becauae the rate or evolution or the PI YUiellOIDeWbatwith different batches or sine, the temperature may be adjuated to obtalD. red1itbbut DOt too violeat. evolution 01.... The'" tube .houJcl be wubcd witecoaceDtrated h,drochlorlc acid, riueCl with water, aDd dried betweea ~.teItI.

B-I.s Jtnpan....., ltaallaftlltala - Mix together 50 mI of water,10 ml of stannated hydrochloric acid and one miUilitre or dilute IOlu"of aneDic. Treat the resulting solution as described UDder D&2 toprepare the standard stain.

A.PPBNDIX B[ TuZ, 1, 1"", (vi) ]

D_A110N 01' COPRa

11-1. UAGDrnIl-I.l '-m B,-...w. - CODCeDtrated.

15

11,171- 1174

8-1.2 c.lMa TetnelalwWe - recliatiUecl before use,

B-I.s II."'D. 801.... - Diuolve 200 I or reagent grade ammoniumcitrate and 50 I of reagent grade dUodium salt or ethylenediamine tetra­acetic acid (Vonene) in distilled water in a one-litre volumetric fIuk.DUute to volume with diltilled water and mix thoroughly.

8-1.t lea.,••Coppe loIatl...

8-1.f.l Slid So,,,'iMI- Tranafer quptitative1y 0·2015 B or rea-,entgrade anhydrous copper sulphate ( CuSC!>a) to a one-litre volumetric ftuk.Dilute to volume with distilled water and mix thoroughly.

1l-1.4.2 $11III4.4 Di"", SoW. - 2 IC/ml. Pipette 25·0 m1 or stockIOlution into a one-litre volumetric flask; dilute to volume with dUtllledwater aDd mix thoroughly. Prepare frahly.

B-l.5 CnMI RecI.lacll.__ - 0-1 percent IOlution.

Il-IA Car___ ReaJeat - 0-1 percent IOlution. DillOlve 0·2 I oflOClium diethyl-dithiocarbamate in distilled ~ater. dilute to 200 m1 volumeand mix thoroughly. Filter, if the lolution il bazy. Prepare fresh daily.

8-2. PROCBDUIlB

1I-2e1 WeiSh accurately 2 «of the aample and tr.Dlrer to • 250-ml~ratinl Funnel. Add mltaUed water to make a total volume 0(25 ml;add 10 m1 of mukin. 101ution Uld 2 cIropi of indicator and miseNeutralize to purple colour with concentrated ammonium hydroXIde andadd 2 drops in esceu. Add 10 ml ofcarbamate reagent and mix. Extractwith 5-ml portioDi of carbon tetrachloride until the final extract i.colourlaa (four extractions are uauaUy adequate). Dry the combined_tracts with reaaent grade anhydroUi lodium lulphate. Filter the dryestract an4 wasb the filter pper with carbon tet...cbloride. Make thevolume of the filtrate to 25 ml with carbon tetrachloride and measure theabIorptiOD at' 433 DID ia to 111m cell. Simultaneously, carry out blankdeterminations on water aDd the reagentl. Extract With S-ml portions ofcarbontetrachloride, and dBute combined extracts to 25 ml with COl,;the colour of the lO1utipniI Dot deeper than that of jhe carbontetrachlorideatractl deri)'ed from s-mf of ltaDdarcllOlutiOD, when aimilarly treated.

8-2-2 Prepare a lelia of ltaDdardi by treating aliquot. of the ltanrlarcldilute IOluti~D (B-l.~) of copper in the same manner al the teatIOlutioft. Prom the ablOrption of the Itandard solutioDi. pre~e aItaDClard curve plottiDl ablo~tioD values apinst conCeDtratiOD. 'romtbiI curve, obtaill the mall of copper.

16

111171 ·1t7t

APPENDIX r[ Tablll, 111m (vii) ]

DBTBRMINA.TlON OF J.LU)

~-1. IlBAGBNTI - All reagents should be free (rom lead.

'-1.1 BJelroclalonc A.cld- 32 percent III/W.'-1.2 ......' ..01 BI•• Sol.dea - Warm 0'1 B of bromoth~l~lue with 3'2 ml of N/20 lOdium .hydroxide and 5 m1 or alCohol\ 90 pereent ): after solution is effected. and sufficient alcohol (20 percaat)to produce 250 mi. .

P-IeS AmID'" 801ad.. StI'ODl - contains not leu than 27 percent-,. and not more than SO percent fII'm of NH•.

P-l.4 25 Pereeat Cltftc Add Solatloa AclJuted to 11-1,B - Dwolve25 g citric acid in minimum quantity of water, cool and slowly add ItroDlammonia solution to acljust 1\1 pH and make total volume to 100mi.

P-l.5 Pota••1IIIl Cyaalde 8olatlo. - Dissolve 10 g of potaaiumcyanide in 90 ml of water, add 2 mJ oC hydrogen peroxide lolution( 6 percent _/" of H,O. ). Allow to stand for twenty four hours. and makeup to 100 ml with water. Mix 2 ml with 5 ml of ammonia IOlution Ptt

and 40 ml of water and add 5 ml of dilute lead solution PI; no darkeningis produced.

P-l.6 D1phea)'lthlocarba... Ia Chloroform - 0'002 perceat ./,1.P-l.7 Nitric Add 8olvdoD - 1 percent II/V, prepared by diluting I mlof concentrated nitric acid (69 to 71 percent ml", HNO.) to 100 ml withwater.

F-l.l AllUDoaI... Cltnte SoI.doD - Dissolve with cooling, 500 I ofcitric acid in a mixture of 200 ml of water and 200 mI of stroDiammonia soluti- .n. Filter and add sufficient water to produce 1000 mi.1'-1.9 L_d 801.tlO8, Str_l- Dissolve 0'16 g lead nitrate in S ml of .concentrated nitric acid (69 to 71 percent ",'v H~O. ) and add auflicientwater to produce 100 mi.

1'-1.10 Lead 801ad... Dnate - Dilute 1 ml or strong lead solution withlufficient water to produce 100 mi.

1'-2. PROCEDURE

~.2.1 Dislolve 10 I of the mat~rial in 20 ml of water, ira..,. to •leparatinl runnel wi~h the aid 01 ~ m1 o~w.tert acid 2'5 mI orhydrocbloric

17

111171- 1174

acid and lhake for five minut... Aclcl 5 drops of bromothymol bluelO1ution and lufficient Itrong ammonia solution to produce a full bluecolour and add 1·5 ml in exceu. Add I ml of a solution prepared bypreviously adjusting a 25 percent _/, solution of citric acid to pH 11 with.tro~ ammoQia solution. 1 ml of potauium cyanide IOlutioD andlufticlcnt of a 0·002 percent miD solution of diphenylthiocarbazone inchloroform until on shaking. the chloroform layer becomes purple or blue.Separate the chloroform layer and add to the aqueous layer 2 ml of thediphenylthiocarbazone solution. shake and separate the chloroform layer.To the combined chloroform solutionI add 10 ml of a 1 percent (,I')solution of nitric acid, shake until the chloroform layer becoma green,allow to separate and discard the chloroform layer. To the aqueouslayer add 0·2 ml of t.he ammonium citrate sclution, 0-25 ml of Itrongammonia solution and 0·2 ml of potassium cyanide solution and sufficientof the diphenylthiocarbazone solution until on shaking, the chloroformlayer becomes purple or blue. Transfer the contents of the .eparatingfunnel to a stoppered tube. Repeat the operations omitting the substal)ceunder examination and using the same quantities of the reagents, 'To thetube containing the blank, add dilute lead solution until on shaking, thecolour of the chloroform layer matches that of .the chloroform layerobtained from the substance under examination. Not more than 2-0 mlof dilute lead solution i. required. The limit prescribed in the materialspecification shall be taken as not having been exceeded if the jlltenlityof itl colour produced in the test with the material is not more than theone produced with the corresponding lead solution.

APPENDIX G( CIGUS, 6.1 )

SAMPLING OW UQ,tJID GLUCOSB

0.1. OBNBRAL UQ,UlRBMBNTS rOR SAMPLtNG

0.1.0 In drawilll. preparing. storing and handling aamples, the followingprecautionI and dlrections shall be observed.

0-1.1 Sampliq iDItrUment ahall be dean and dry when uaed.

0-1.2 Precautions mall be taken to draw the lample. in a manner 10 Uto protect the .amples, the material being sampled, the Ial1)plinlWtrumODt and the containerl for samples from adventitiouscontamination.

G-Iel The aampla ahall be placed in clean and dry glalS eontainen.

J8

1I.1IS-1I7.

Q.IA The ample containen ,hall be I~led air-tisht after fiI1iDI andmarked with full details of sampling, date of manufacture. Dame ormanufacturer and other important particulars of the coDlignment.

0-1.5 Samples shall be stored in such a manner that the conditioDi ofstorage do not unduly affect the quality of the material.

G-2. SCALE OF SAMPLING

0.2.1 Lot - All the containers in a lingle consignment of the materialof the same grade, drawn from a lingle batch of manufacture ahallcoDititute a lot. If a consignment is declared to consist of different batchesof manufacture, the containers belonging to the same grade and batchshall be grouped together. Each group shall constitute separate loti.

G4.2 On•• Sample - For the purpose of drawing samples for test, onecontainer from each lot shall be selected by the purchaser. This contaiDershall constitute the grOIl sample.

0-2.2.1 The container selected for the gross sample shall not be damagedor leaky and shall have its seal intact.

0-,. PROCEDURE FOR DRAWING A SAMPLE FROM TIlECONTAINER

G-I.l S.m...... Pa•• - For the purpose ofdrawing a sample of liquidJrlucose from the container, it is recommended that a pump of the typeshown in Fig. 2 may be used. The pump is made of copper. The tubeof the pump is 2 mm thick. with an internal diameter of 42 mm. Thetube i. provided with a downward bent spout at 76 mm from the upperend of the screw top. The spout i. of 19 mm internal diameter. Thepiston is provided with lifting up valves. The piston rod is 8 mm indiameter and i, made or copper. The rod is provided witb a lockingdevice at itl top 10 that it may not have to be kept preaed down while thematerial rises up inside the tube.

Q.J.2 Method ., lampUaI- Place the container selected for thepurpose or .ampling at a clean and dry place. Thoroughly clean the lidof the opening or the container and the area round about the openingwith IO&p and water. Wipe the cleaned area dry. Remove the lid orthe opening and take out with a ladle sufficient quantity of the materialto remove the top scum. if any. Insert the pump (s" G-S.l) with itlpilton locked in positiOD. throulfh the openinR of the container and veryIlowlypresl the pump down to the extent of the upper one-third height ofthe container. Hold the pump in thia position for five minutes. ARAin veryslowly preal the pump down through the middle one-third he~htofthe con­tainer and hold the pump in tbat poaition for five minutea. Prell the pumpdown again VPry Ilowly and when it touches the bottOID of the container.

It

18 I 873 • 1974

LOC'"DIYC.

••gENLARGED SECTION AT X X

LOCKiNG DEVICE

............-.DI.

AlldilllCDlbaa AD aaiIIimelftl.

Flo. 2 SAalPuNo Pmo20

111171 • lt7t

raile it a little 10 that it is just above the bottom of the container. Allowthe pump to remain in that position again for five miDuta. (The pumpwill have been filled in by thia time. ) Take out the pump aDd .wipe itaoutside surface 10 as to remove the material which may be adheriDg to it.Unlock the piston and work it very slowly through one complete upwardmovement, simuJtan:ously, collecting the material flowing out of thespout in a perfectly clean and dry sample container. Repeat the operationof drawing samples in the above manner, until a quantity of about 2 kg ofthe material is collected in the sample container.

G-4. TEST SAMPLES AND RBI'ERBE SAMPLE

G-f.l .........do. 0' Te8t ....pl.. - To prepare test samples,thoroughly mix the material drawn as specified under G-S.2 and divide itinto three equal parts, each part ( test sample) being not lesa than 0-6 kg.Transfer these teat samples to thoroughly clean and dry sample containers,and seal them air-tight. Label the sample containen with all theparticulars given under 0-1.4. One of these samples shall be lent to thepurchaser and one to the vendor.

G-t.2 Rei.... ....pl. - The third sample, bearing the seals of thepurchaser and the vender, shall constitute the referee aample to be uaedm case of dilpute between the purchaser and the vendor. It shan be keptat a place agreed between the purchaser and the vendor.

G-5. _ O:r TaTS AND CRITBIUA :rOil COIUOallllY

0-5.1 The test sample as prepared under G-U shaD be tested tor all· characteriatiCl ('11.) of this lpecification.

G-5.2 The lot .hall be cleclared as conforming to this lpecificatioD if thetest raulta aatilfy the requirements or each of the characteriltia.

21

BUREAU OF INDIAN STANDARDS

H••dq.." ....Manak Bhavan, 9 Bahadur Shah Zafar Marg, NEW DELHI 110002Telephones: 323 0131, 323 3375, 3239402 Fax:+ 91 011 3234082, 3239399, 3239382E • mall. bis@Wnl.com. W.bslte : http://www.bis.org.in

550 1348

8394955

32378 17

3378662

6038 43

254 13 15

832 92 95

52 51 71

21451

262808

421.58

3712 51

403627

218835

542 82 61

471 1988

Sinha Path, 54 11 37

C."".", LIIbotatOt'Y :Plot No. 2019, Si18 IV, Sahibabad Industrial Are.. s.hib8b8d 201010II.,., otfIt:_:

Central: Manak Shav.", 9 Bah.tur Shah lata, Marg, NEW DELHI 110002

*Eastern : 1/14 CIT Scheme VII, V.I.P. Ao8d, Kankurgachi, KOlKATA 700054

Nor1hem : SCO 335-336, Sector 34-'" CHANDIGARH 180022

Southern: CJ.T. Campus, IV Cross Rold, CHENNAI600113

tWestem : Manatc.laya, ES,MlDC, BehindMarol Telephone Exchange,Andheri (East), MUMBAI 400093

Bgnch omc_:'Pushpak', Nurmohamed Shaikh Marg, Khanpur, AHMEDABAD 380001

Peenya Industrial Area, 1at Stage, Bangalore-Tumkur Road,BANGAlORE 560058

Commercial-eum-Offlce Complex, Opp. Dushera Maidan, E-5 Are,. Colony, 723452Bittan Market, BHOPAL 462016

82J63, Ganga Nagar, Unit VI, BHUBANESWAR 751001

5th Floor, Koval Towers, 44 Bala Sundaram Road, COIMBATORE 641018

Plot No. 58. Neelam Bata Road. NIT, FARIDABAD 121001

S.vttrt Complex. 116 G.T. Road, GHAZIABAD 201001

53IS Ward No.29, R.G. Barua Road, 5th By-lane, ApurbaGUWAHATl781003

5-8-58C, L.N. Gupta Marg, Namp8lty Station Road, HYDERABAD 500001 320 1084

E-52, Chltr."jan Maarg, C- Scheme, JAIPUR 302001 3738 7SJ

117/418 B, Sarvodaya N8gar, KANPUR 208005 218878

Seth Bhawan, 2nd Floor, Behind Lee" Cinema, Nav.. Kiehore Ro.., 21 8923LUCKNOW 226001

NIT Buldlng, Second Floor, Gokulpat MBrket, NAGPUR 44001j;1.Mahabir Bhaw",I.t Floor, Roper Roed. NA~RH 174101 _.

PatHputra Indus"'" Eatate. PATNA"11" .Rrst Floor, Plot"'. 857-860, MarketYard,Gullekdl. PUNE411037

~ 1-b,Jee' 3RtFloor,~ CIrcle, 80 FMtRoM. ~' .RAJKOT 38OOQ2 \

T.e. No.14/1C1. t.HwnIIyP. o.~.1HRtN_ 88!034 32 21 04

*SaIee OffIce '- at 5 ChowrtnghM~,~ O. Prlncep StrMt,KOLKATA700072

ts-..~ .. at Nowtty a-ma.r., Grent Ao.d, MUMSAI400007

2371086

3011521

PrInted at NM IndIIl PrInting Pr••, KhU.... , Incla