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है”ह”ह

IS 15740 (2007): OIL OF ROLSE [PCD 18: Natural andSynthetic Fragrance Materials]

IS 15740:2007

,.

Indian Standard

OIL OF ROSE — SPECIFICATION

ICS 71.100.60

@BIS2007

BUREAU OF INDIAN STANDARDSMANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG

NEW DELHI 110002

January 2007 Price Group 2

Natural and Synthetic Fragrance Materials Sectional Committee, PCD 18

FOREWORD

This Indian Standard was adopted by the Bureau of Indian Standards, after the draft finalized by the Natural andSynthetic Fragrance Materials Sectional Committee had been approved by the Petroleum, Coal and Related ProductsDivision Council.

Rose plant is grown in Bulgaria, Turkey, Morocco, USSR, Spain, Germany, France, Iran and India. In India, the mainareas of its cultivation are located chiefly in Uttar Pradesh, Rajasthan and to some extent in Punjab. Alkaline saline soilswith pH range of 8-9 are quite suitable for the growth of rose plant. This plant flowers during the month of March andApril in North Indian Plains and May and June in hilly areas. Rose oil is used extensively in flowering, perfumery andmedicinal purposes.

In the formulation of this standard, considerable assistance has been derived ffom testing experience of fragrance andflavour industries.

An International Standard ISO 9842:2003 ‘Oil of rose (Rosa damuscena Miller)’ also exists, which is not equivalent toIndian variety.

For the purpose of deciding whether a particular requirement of this standard is complied with the final value, observedor calculated, expressing the result of a test or analysis shall be rounded off in accordance with IS 2: 1960 ‘Rules forrounding off numerical values (revised)’. The number of significant places retained in the rounded off value shoulp bethe same as that of the specified value in this standard.

u!!!!!IS 15740:2007

Indian Standard

OIL OF ROSE — SPECIFICATION

1 SCOPE

This standard specifies the requirements and the methodsof sampling of essential oil of rose (Rosa damascenaMiller),

2 REFERENCES

The following standards are necessary adjuncts to thisstandard:

IS No.

326

(Part1):1984(Part2) :1980

(Part 3): 2006/1S0279 :1998

(Part4): 2005/ISO592:1998

(Part 5): 2006/ISO280:1998

Title

Methods of sampling and test fornatural and synthetic perfumerymaterials:

Sampling(second revision)

Preliminary examination of perfhmerymaterials and samples (secondrevision)

Determination of relative density (thirdrevision)

Determination of optical rotation (thirdrevision)

Determination ofrefiactive index (thirdrevision)

(Part 18): 1984/ Determination of fieezingpoint (second

ISO 1041:1973 revision)

(Part 19): 1998 Gas chromatographic analysis ofperfimery materials

1070:1992 Reagent grade water (third revision)

2284:1988 Method for olfactory assessment ofnatural and synthetic perfumerymaterials (first revision)

6597:2001 Glossary of terms relating to flagranceand fiavotir industry (second revision)

3 TERMINOLOGY

For the purpose of this standard, the definitions given inIS 6597 shall apply.

4 REQUIREMENTS

4.1 The material shall comply with the requirementsgiven in Table 1.

4.2 Chromatographic Profile

Analysis of the essential oil shall be carried out by Gas

1

Chromatography (see Annex A). In the chromatogramobtained, the representative and characteristiccomponents shown in Table 2 shall be identified. Theproportions of these components indicated by theintegrator shall be as shown in Table 2. This constitutesthe chromatographic profile of the essential oil.

5 PACKING AND MARKING

5.1 Packing

The material shall be supplied in airtight containers,preferably glass, tin-lined or aluminum, permitting aminimum of airspace, as agreed to between the purchaserand the supplier.

‘ 5.1.1 Direct contact of the material with the galvanized ormild steel containers shall be avoided.

5,1.2 The material shall be protected from light and storedin a cool and dry place.

5.2 Marking

Each container so packed shall bear legibly and indeliblythe following information:

a)

b)

c)

d)

e)

f)

g)

Name of the material;

Manufacturer’s name and address and/or hisrecognized trade-mark, if any;

Net and gross mass of the material;

Indication of source of manufacture;

Batch number;

Month and year of manufacture; and

Cautionary notice, if any.

5.3 BIS Certification Ma,rking

The containers may also be marked with the StandardMark.

5.3.1 The use of the Standard Mark is governed by theprovisions of the Bureau,of Indian Standards Act, 1986and the Rules and Regulations made thereunder. Thedetails of conditions under which the licence for the useof the Standard Mark may be granted to manufacturer orproducers may be obtained from the Bureau of IndianStandards.

6 SAMPLING

Representative samples of the materials, each sample

EIS 15740:2007

f,,

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Table 1 Requirements for Oil of Rose(Clauses 4.1 and 7.1)

SI No. Characteristic Requirements Method of Test,Ref to 1S

(1) (2) (3) (4)

1) Appearance Mobile and clear liquid 326 (Part 2)

ii) Ccdour Colorless to light yellow 326 (Part 2)

Iii) Odour Characteristic rosy 2284

iv) Relative density l]:

a) at 20°C 0.8700 to 0.8800 326 (Part 3)

b) at 27°C 0.8500 to 0.8750

v) Refractive indexll

a) at 20°C 1,4520 to 1.4680 326 (Part 5)

b) at 27°C 1.4560 to 1.4700

VI) Optical rotation – 2° to -4.5° 326 (Part 4)

vii) Freezing point, “C 9 to 12 326 (Part 18)

viii) Congealing point, ‘C 20 to 30

II Re[atlve density is synonymous with ‘specific gravity’. The correction factors for relative density and refractive index for each

degree Celcius change in temperature are 0.00064 and 0.00038 respectively,

containing not less than 50 ml shall be drawn as prescribed water (see IS 1070) shall be employed in tests.is [S326 (Part 1).

NOTE — ‘Pure chemicals’ shall mean chemicals that do not

contain impurities, which affects the result of analysis.

7 NUMBER OF TESTS

Table 2 Chromatographic Profile7.1 Test for the determination of all the characteristics (Clauses 4.2 and7. 1)shall be conducted on the composite sample as prescribedin 4.1,4.2, Table I and Table 2. S1 No. Components Minimum, Maximum,

Percent Percent

7.2 Test Methods (1) (2) (3) (4)

7.2.1 Tests shall be carried out as per the relevant Indian i) l-Citronellol 22 32

Standard. ii) Nerol 10 14

iii) Geraniol7.3 Quality of Reagents

23 32

iv) Phenylethyl alcoholUnless specified otherwise, pure chemicals and distilled

3 7

1S 15740:2007

ANNEX A(Ckum 4.2)

GAS CHROMATOGRAPHIC ANALYSIS OF OIL OF ROSE

I,’

, I

A-1GENERAL

The chromatographicanalysis is given on capillary column.The chromatographic conditions given here are forinformation and guidance.

A-1.1 Outline of the Method

A sample of the material is dissolved in a suitable solvent(for example, hexane, cyclohexane or petroleum ether) andis injected into the gas chromatographywhere it is carriedby the carrier gas from one end of the column to the other.During its movement, the constituents of the sampleundergo distribution at different rates and ultimately getseparated from one another. The separated constituentsemergefromthe endof the columnone afieranotherandaredetectedby suitablemeanswhoseresponseis relatedto the amount of a specific component leaving the column.

A-2 PROCEDURE

A-2.1 The analysis shall be done as per IS 326 (Part 19).The typical chromatograms for oil of rose in capillarycolumn with the following chromatographic conditions isshown in Fig. 1.

A-2.1.1 Gas Chromatographic Conditions of CapillaryColumn

Column : Fused silica capillarycolumn

a) Size : Length, 30 mlntemal diameter, 0.23 m

b) Carrier gas : Nitrogen

Stationery phase : Carbowax,20 m

Conditions

Initial temperature : 50°c

Ramp/Temperature : 4.5 OC/minprogramme

Final temperature : 200“c

Initial time : Smin

Injector port temperature: 210 T

Detector

a) Type : Flame ionization

b) Temperature : 220”CVolumeinjected : 0.2 pl

A-3 CALCULATION

A-3.1 Area Measurements (see Note 1)

Since normal peaks approximate a triangle, the area ismeasured by multiplying the peak height times the widthof the half height. The normal peak base is not taken sincelarge deviations may be observed due to tailing oradsorption. This technique is rapid, simple and fairlyaccurate when peaks are symmetrical and of reasonablewidth.

A-3.2 Area Normalization (see Note 2)

By normalizing, it is meant calculating the area of each anddividing the individual areas by total areas,

Percentage ofA =Area of A x 100

Total area

NOTES

1 Other methods of area measurements, namely, triangulation,

disc integrator and electronic digital integrator, if fixed with

GC machine, would be of great advantage.

2 Internal standardization can be used, if pure appropriate

internal standard is available. This method is known as relative

or indirect calibration.

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Bureau of Indian Standards

BIS is a statutory institution established under the Bureau of Indian Standards Act, 1986 to promoteharmonious development of the activities of standardization, marking and quality certification of goods andattending to connected matters in the country.

Copyright

BIS has the copyright of all its publications. No part of these publications may be reproduced in any formwithout the prior permission in writing of BIS. This does not preclude the free use, in the course of implementingthe standard, of necessary details, such as symbols and sizes, type or grade designations. Enquiries relating tocopyright be addressed to the Director (Publications), BIS.

Review of Indian Standards

Amendments are issued to standards as the need arises on the basis of comments. Standards are also reviewedperiodically; a standard along with amendments is reaffimed when such review indicates that no changes areneeded; if the review indicates that changes are needed, it is taken up for revision. Users of Indian Standardsshould ascertain that they are in possession of the latest amendments or edition by referring to the Iated issue of‘BIS Catalogue’ and ‘ktandards: Monthly Additions’.

This Indian Standard has been developed from DOC: No. PCD 18 (1855).

Amendments Issued Since Publication

Amend No. Date of Issue Text Aflected

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