Invitrogen RNA Ordering Guide - Thermo Fisher Scientific · 2020-04-21 · Invitrogen RNA Ordering...

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Invitrogen RNA Ordering Guide Rev. September 2013

Transcript of Invitrogen RNA Ordering Guide - Thermo Fisher Scientific · 2020-04-21 · Invitrogen RNA Ordering...

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Invitrogen RNA

Ordering Guide

Rev. September 2013

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Invitrogen RNA Ordering Guide

This guide describes how to order RNA in Lifetechnologies website. Before ordering RNA, you

need to create Lifetechnologies user account. Chapter 1 describes to create user account. After

finishing registration, you can place order RNA through Lifetechnologies website.

Please refer to our website for more information of Invitrogen RNA as below.

RNA Order Top Page

http://www.lifetechnologies.com/jp/ja/home/life-science/rnai/rnai-email-order-forms.html

We recommend PC and browser condition as below. We recommend Google Chrome strongly.

Windows

Internet 7-8

Google Chrome

Firefox

Macintosh OSX

Safari

Google Chrome

Firefox

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Table of contents

1. Login and new registration ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ P3

2. How to order RNA ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ P9

3. BLOCK-iT™ RNAi Express ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ P21

4. Stealth RNA New Search Site ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ P24

5. BLOCK-iT™ RNAi Designer ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ P37

6. Ordering Synthetics for in vivo RNAi ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ P54

7. Plate select ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ P57

8. My account ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ P63

9. FAQ ・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ P70

Contact us if you need more help to order custom primers.

Lifetechnologies Japan

Order support Invitrogen custom primer order team

Tel: 03-6832-6980 (Invitrogen Primer team) Guidance 1 to 2

Email: [email protected]

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1. New account registration and log into your account

Go to Lifetechnologies website to http://www.lifetechnologies.com/

When you access Lifetechnologies website in Japan, you see Japanese flag at the upper left.

If country is not Japan, you can change county.

Click ”Order Support” .

Click ”Change location)”.

Click “>Japan|日本” .

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You can change language to use a pull-down menu at the bottom right. We have not finished

Japanese translation yet and there are a lot of pages only Japanese. Sorry for the

inconvenience.

Before you place order primers, you have to log into your account first.

Point the cursor to サインインする and click ログイン. If you don’t have web order account,

click ”ここから開始します” to create new account.

Enter user account as Email address and password, then click サインイン. You can store user

account and password to click □ on the left of ”保存する” When you forget username or

password, click ”サインインにお困りですか?” or”パスワードをお忘れですか?” and follow

the instruction.

After login to your account, you can see your name.

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New customer registration

You can register new customer registration on website. Enter email address which is used as

login ID and other information. Marked (*) columns are essential for new customer registration.

After you complete registration on website, you will receive email to notice registration of your

account from Lifetechnologies within a day when you register by the evening. If you register

new account at night or weekend, you will receive notification mail the day after registration.

After you get notification mail, you can place the order.

Enter email address, password, security question and answer. Marked (*) columns are essential

for new customer registration. This email address will be the username for your account, as

well as the destination for order-related emails. We recommend Password

Strength is high as green line.

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Select “はい” or “いいえ” whether to receive membership email. Then read Life Technologies

General Terms & Conditionsand Terms of Use and check and click/check at the left of ”ライフテ

クノロジーズのウェブサイト利用条件および標準販売条件に同意します”.

If you place primer order soon, click “はい、登録を続けます” and continue to enter shipping

information. When you click “いいえ、登録をここで終了します”, only email address as userID is

saved. You can add shipping information at the first primer order.

Enter contact information.

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Enter address for shipping.

No need to enter account number if you have no information.

Next you select dealer. Select prefecture from pull-down menu and click search. You see dealer

list and select one dealer you will contract. If you know dealer company name, you entre

dealer name in dealer name field and you see selected dealer list.

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Decide contract dealer and click ○ to select dealer and then click 送信. If you can’t find dealer

name, click ここをクリックすると”.

Enter dealer name and address. Select Japan in country field.

Select yes/no in membership benefit field. The You will receive technical updates from Life

Technologies if you select yes. This mail doesn’t contain order information. Customer s will

receive order confirmation email without relation to this selection.

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Take a few minutes to read and accept Life Technologies and click □ at the left of I accept after

you accept the Terms and Conditions. Finally click 登録する to register.

You see thank you message as below and you receive auto-reply mail to receive your request. If

you select dealer and enter both ship to address, we start your registration. You receive mail

about completion of user registration within a day when you submit the request by 4pm,

Monday to Friday. If you subimit the request after 4pm or weekend and holidays, you will

receive mail about completion of user registrataion on the following day. If you need urgent

request, please contact us on page 2.

Thank you message

Auto reply mail

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2. How to order RNA

Go to primer order website as below.

1.Point the cursor to ライフサイエンス and click RNA 干渉 Oligos

2. Point the cursor to Click オーダー製品 and then click “RNA 干渉 Oligos” as below.

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3.Click ”オーダー製品” and then click RNA 干渉 Oligos) or ”RNA 干渉 Oligos” as below.

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Select RNAi order as below.

Basic Entry Enter one primer sequence at a time.

Upload Entry Upload multiple sequences at once.

BLOCK-iT™ RNAi Express Search pre-desinged RNA and order it on website

BLOCK-iT™ RNAi Designer Design DNA sequence to insert in RNA expression vector, order

Dye-labeled siRNA, in vivo grade RNA and order RNA in plate.

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1. Basic Entry

Click 基本入力注文を to enter basic siRNA. Click ”Stealth ベーシックエントリー” to enter

Stealth RNA(25mer). You can change siRNA/steahlth RNA at next screen.

Enter sequence and other information as guidance. When you select siRNA in Type of RNA

menu, synthesized RNA is unmodified double strand RNA. When you select Stealth RNA,

synthesized RNA is chemical modification of Stealth RNA. Please note that Lifetechnologies

offer only double strand RNA and click□ at the left of “Duplex” and enter check when in place

order.

Enter 19mer sequence and select dTdT using the 3’ overhang pull down menu if you need

siRNA with ’ Overhang dTdT. Enter 25mer sequence and select “None” in 3’ overhang.

Please note that chemical modification of Stealth RNA is done by only sense strand and if an

antisense strand is ordered as a sense strand, can't get any knock down effect. We recommend

you to confirm before order, sense and antisense. After completing to enter sequence and

other information, click カートに追加する.

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To continue order, enter sequence and needed information again. To confirm primer

information in cart, click カートを見に行くかを or click cart at the top right.

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Click →注文へ進む to continue order processing. Click RNA name or 編集 If you need to

edit primer information.

If you account has multiple dealer account, you can select dealer from pull-down menu for

each products.

Click 代理店変更.

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Select dealer from pull-down menu.

You can change dealer by each line. Or click カート内すべての品目に適用 if you want to

change dealer for all products.

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Confirm your billing and shipping addresses. Note that name fields followed by an asterisk (*)

must be filled in. Enter some characters such as (*) if you don’t know dealer name. Then click

確認を続行>.

When you select multiple dealers in one order, you need to confirm billing and shipping

information separately. After you confirmed data first, click continue (1 of 2) to confirm next

information.

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Leave check in □ at the left of “全てのオリゴをまとめて出荷” because all RNA products are

made in US.

Uncheck □ at the left of “カスタム製品の設計/シーケンスの詳細を全ての E メール、請求書、

及び出荷文書に表示しない”If you need sequence information in order confirmation email. To

continue this option, check □ at the left of “このオプションを今後の注文にも反映する”

Enter your original order number. You can enter any number, but this field must be filled in.

Enter any characters like (.) even if you don’t need your original order number.

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When you have any special instructions to specify the date of delivery and so on, click ▼ at the

right of “特記事項”. You can see free space as below and send us special instructions.

After finished entering data, click 確認を続行> at the top or bottom of the right and then

proceed final review screen. Click 注文する> to complete order. Please note that you can’t

cancel the order after you submit order.

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Confirm order details as below. You also receive order confirmation email. Please keep in mind

that order number such as 784XXXXX is very important to ask inquiry.

If you selected multiple dealers in one order, order confirmations including order number are

created separately as below.

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2.Upload Entry

Click アップロード注文 to upload multiple unmodified siRNA. Click ”Stealth アップロードエント

リー” to upload Stealth RNAi siRNA. You can change siRNA/Stealth RNA at next screen.

Download template first to click”シーケンステンプレートをダウンロード”.

Template file contains two worksheets for siRNA(RNAi Template) and Stealth RNA(Stealth

Template). Input sequence and other information and save the file. We recommend to save as

Excel book(.xlsx) or Excel97-2003(.xls). When you save file as txt, select worksheet first.

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Next click ファイルを選択 and select saved file. And then select RNA Type from pull-down

menu and click アップロード.

You can copy contents of row A to G in template file and paste information on website directly.

After pasting information, select RNA Type from pull-down menu and then click submit.

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After uploading file, turn to next step as below. Marked “●” shows there are no problems for

primer order. Marked “●” +”x” shows this information contains any error and not proceed to

next step and you have to edit and fix detailed information. If you don’t need primers, click 削

除 for each primer. To order additional primers, click 再アップロードする. After preparing all

orders, click カートに追加する and proceed to cart.

Products are added into cart and proceed to ordering step according to Page 15-20.

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3.BLOCK-iT™ RNAi Express

Search pre-designed Stealth RNA, Stealth Select RNA, miRNA expression vector and orders

these products in BLOCK-iT RNAi Express.

Login first and access to BLOCK-iT RNAi Express site

http://rnaidesigner.invitrogen.com/rnaiexpress/rnaiExpress.jsp

In default screen, all species and products are checked and click and uncheck if you want to

exclude any species and products. Refer to guarantee of Stealth and Stealth Select RNA as

below link.

Specification Chart and Guarantee

http://rnaidesigner.lifetechnologies.com/rnaiexpress/spec.jsp

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Start to search by ID/Keyword (Default) by ordinary. There are some pathways or gene families

in each menu and change search method if you need.

In step2, select search type from a pull-down menu. Default menu is search by Gene Symbol.

Then enter keyword and click Submit to search.

Search result is displayed check products you need. Then select Tube and click Add to Shopping

Cart to proceed to ordering step. Check ○ Plate if you desire RNAs in plate. Refer to Plate

Select in XX about plate order. If you need 3 Stealth Select RNA for same target gene, click and

check the top line of Stealth RNAi. You can order 3 siRNAs simultaneously. Or click each line

and you can select each siRNAs separately. Click hyperlink of each RNA and refer to detailed

information.

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Enter researcher name at next screen and click Order to put products in cart. Not available web

order of Controls under selected siRNAs and don’t check Control products.

Add products in cart and continue order process according to page 15-20.

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3. Stealth RNA New Search Site Lifetechnologies introduces new designed search site of Stealth RNA. New site contains some

useful information related to NCBI links, graphical mapping display and so on. After integration

of Applidbiosystems user account planned in future, customers can search and order

Appliedbiosystems products as taqman assay or ambion scilencer siRNAs via Lifetechnologies

website.

Go to new search site as below,

1. Point the cursor to ライフサイエンス and click RNA 干渉. as below.

Click siRNA as below.

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Currently there is only Stealth RNAi icon when login in Japan. Search icons about

Appliedbiosystems taqman assays and ambion siRNA are not shown. After unification of

Appliedbiosystems and Lifetechnologies user account, customers in Japan will be able to search

and order Taqman and Ambion siRNA products in Lifetechnologies website.

2.After click siRNA, click 詳細はこちら> on Stealth RNAi siRNA or 今すぐ注文する in Stealth

RNAi siRNA.

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Search Stealth RNAi siRNA

Click species for interest. You can select multiple species.

Enter keyword in keyword field. When you begin to enter keyword, you see some keywords

contain word with auto complete function and select one. Or after you enter keyword, click 検

索.

When you search with multiple keywords and IDs, click Enter/Upload Multiple Targets first.

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Enter keywords in Option1 space. You have to separate keywords with a space, comma, or

press enter after each keywords. Supported keywords or IDs are shown at the right of space.

You can also upload keyword file in Option 2. Click ファイルを選択する in Option 2 and select

file and click OK and click 検索. Supported file format is shown in Option2 field.

Search results are displayed.

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Click View Details to get more information about target gene. Click accession number to check

NCBI database information. Click View Full Product Details on the bottom left to see all detailed

information on another window.

Click Controls to see siRNA control products. But controls are catalog products and you can’t

place order via website. Please click Product Name to confirm product number and order

product via dealer representative.

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Click View siRNA on Map for each siRNA to get graphical information about siRNA position.

Red icon shows siRNA of selected. You can also get siRNAs for same gene target. “St” means

stealth RNA and “S” means scilencer siRNA (ambion).

Click Close Map to close graphical display.

After reviewing detailed information of each siRNAs, click square at the left of siRNA ID and

check to click Add to Cart to add products in cart. If you choice set of 3 siRNA for selected,

don’t click each siRNAs, but click square at left of Stealth siRNAs (Set of 3). When you select

each 3 siRNAs, discount price is not applicable.

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Click →カートを見る at the top of right and proceed to next step. Products are added into cart

and proceed to ordering step according to Page 15-20.

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Select some siRNAs and click Compare to compare siRNAs you selected. You also can get

selected siRNA information. Click Email to get summary by email or Export to export tables

directly.

If siRNA sequence is 100% match for other species targeted, you see notification as below and

click it to get more detailed information. When this siRNA is not allowed to your experiment,

don’t select this siRNA.

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Menu at the left side is as below,

Change Your Search New assay search

Narrow Your Results Limit marked species about your search results

Reset Filters Reset your search Filters

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5.BLOCK-iT™ RNAi Designer

Design custom siRNA for target design or design insert double strand DNA for expression vector

in BLOCK-iT™ RNAi Designer website.

Login first and access to BLOCK-iT RNAi Designer site

http://rnaidesigner.lifetechnologies.com/rnaiexpress/rnaiDesign.jsp

Select design option first.

Stealth RNAi™ siRNA Design custom Stealth siRNA for target sequence

siRNA Design siRNA for target sequence.

miRNA RNA Design insert sequence for miRNA expression vector

shRNA Design insert sequence for shRNA expression vector

siRNA to Stealth RNAi siRNA Design Stealth RNA(25mer) based on target sequence and siRNA

(19mer)

siRNA to shRNA Select siRNA sequence and vector and design double strand DNA

sequence for shRNA expression vector

Stealth RNAi™ siRNA

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Step1: Enter accession number or sequence.

Step2: Select region for target design.

Step3: Select target species for Blast.

Step4: Set minimum and max %GC.

Then click RNAi Design.

Search result is displayed. Click and check □ at the left of desired sequence. Then click

Continue.

Click star of each line and then refer to details of design rank to evaluate each sequence.

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Definition of Design Rand is as below.

http://rnaidesigner.lifetechnologies.com/rnaiexpress/help/help_for_rnai_designer.htm#probab

ility.htm

Design Rank

The BLOCK-iT™ RNAi Designer will generate up to 10 designs per target sequence. Each design

is given one of the following five Probability rankings:

Designs with this ranking closely fit the characteristics of the most effective RNAi

molecules. They have the greatest likelihood of resulting in >70% knockdown. We recommend

testing 2 or 3 designs from this group.

Designs with this ranking fit most of the characteristics of effective RNAi molecules.

They are very likely to result in >70% effective knockdown. We recommend testing 3 designs

from this group.

Designs with this ranking fit some of the characteristics of effective RNAi molecules.

They are likely to result in >70% effective knockdown. We recommend testing at least 3

designs from this group.

Designs with this ranking have fewer of the characteristics of effective RNAi

molecules but they are still likely to result in >70% effective knockdown. We recommend

testing at least 3 designs from this group.

Designs with this ranking have the fewest characteristics of effective RNAi molecules

but many are still likely to result in >70% effective knockdown. We recommend testing at least

3 designs from this group.

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Select Tube or Plate at below screen and click Order Online. Suitable sequence as negative

control for target sequence is displayed. Check it if you need to order.

Enter Researcher name and select Purity and Scale. Then click Add to Cart.

Products are added into cart and proceed to ordering step according to Page 15-20.

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siRNA

Click siRNA.

Step1: Enter accession number or sequence.

Step2: Select region for target design.

Step3: Select target species for Blast.

Step4: Set minimum and max %GC

Step5: Select motif pattern

Then click RNAi Design to design custom sequence.

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Design result is displayed as below. Click and check □ at the left of desired sequence. Click ★★

to see details of design rank. Then click Continue.

Select Tube or Plate at below screen and click Order Online. Suitable sequence as negative

control for target sequence is displayed. Check it if you need to order.

Enter Researcher name and select Purity, Scale, Overhang and 5’/3’ modification. Then click

Add to Cart.

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Products are added into cart and proceed to ordering step according to Page 15-20.

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miR RNAi

Click miR RNAi.

Step1: Enter accession number or sequence.

Step2: Select region for target design.

Step3: Select target species for Blast.

Step4: Set minimum and max %GC.

Then click RNAi Design to design custom sequence.

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Candidate target sequences are displayed and check □ at the left of sequence you need. Then

click Design miR RNAi.

Double strand sequence as insert for Invitrogen's pcDNA™6.2-GW/miR and

pcDNA™6.2-GW/EmGFP-miR vectors is displayed. Confirm it and click Order Online. Click link

for protocol to get annealing method.

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Enter Researcher name and select 5’/3’ modification, Purity and Scale. Then click Add to Cart.

Products are added into cart and proceed to ordering step according to Page 15-20.

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shRNA

Click shRNA.

Step1: Enter accession number or sequence.

Step2: Select region for target design.

Step3: Select target species for Blast.

Step4: Set minimum and max %GC

Step5: Select vector and Strand orientation

Then click RNAi Design to design custom sequence.

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10 candidate target sequences are displayed and check □ at the left of sequence you need.

Then click Design shRNA Oligos.

Select Loop Sequence using pull-down menu or enter custom Loop Sequence at the right

column. Then click Design.

Double strand sequence for cloning is displayed. Confirm it and click Order Online.

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Enter Researcher Name and select modification, purification and scale from pull-down menu

and click Add to Cart

.

Products are added into cart and proceed ordering step according to Page 15-20.

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siRNA to Stealth RNAi™ siRNA

Click siRNA to Stealth RNAi™ siRNA.

Enter Accession number or sequence, select species for Blast. And then enter 19mer siRNA

sequence and click Submit.

25mer Stealth sequence based on input is displayed. Sequence is DNA sequence. If you need

Negative control which is displayed under 25mer Stealth RNA sequence, check it. Then click

tube or plate as you need and click Order Online.

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Enter Researcher Name and select Purity and Scale from pull-down menu, and then click Add

to Cart.

Added in cart as Stealth RNA (double strand of 25mer RNA) and proceed ordering process as

Page 15-20.

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siRNA to shRNA

Click siRNA to shRNA.

Enter siRNA sequence in Sense siRNA Sequence field and select loop sequence from pull-down

menu of Default Loop Sequence or entre original loop sequence in Custom Loop Sequence.

And the select vector from pull-down menu and then click Design shRNA Oligo.

Insert sequence is displayed and confirm it. Then Click Order Online.

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Enter Researcher Name and select 5’/3’ modification, purification and scale. Then click Add to

Cart.

Products are added in cart and proceed to ordering process as Page 15-20.

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6. Ordering Synthetics for in vivo RNAi

Click Synthetics for in vivo RNAi to order in vivo siRNA

http://rnaidesigner.invitrogen.com/rnaiexpress/

Enter name in duplex name when you select Custom Stealth RNAi siRNA and then enter 25mer

sense strand sequence and Antisense strand sequence. Enter 19mer sense strand sequence

and Antisense strand sequence. Need to enter 100% complementary oligo sequence. If not

complementary sequence, you see error message and not to proceed next step. When you

select custom siRNA, dTdT overhang sequence is added automatically.

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Select purity from a pull-down menu. You can add endtoxin testing to check Endo Toxin Testing

if you select Desalt or HPLC in vivo purity and RNA is shipped after endtoxisin test passed. Then

enter researcher name and click Add to cart.

Enter siRNA ID number when you select Stealth Select RNAi siRNA. Click Find Stealth Select

RNAi siRNA IDs to search ID number of siRNA in Bloclkit Express.

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If you select Negative controls at right side, you can order same sequence of commercial

products and you can select purity and scale.

Products are added in cart and proceed to ordering process as Page 15-20.

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7. Plate Select Customers can order siRNAs in 96 well format using Plate Select. Only 1nmole scale is available

in Plate Seletct format. Click Plate Select as below.

http://rnaidesigner.invitrogen.com/rnaiexpress/

Click Add Plate to make new plate format.

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Enter Plate Name and Researcher Name in English and select siRNA or Steahth RNAi siRNA in

plate type and click ADD. You can’t change purity and scale in plate format.

96well format is displayed and select search menu at the top right. When you select RNAi

Express or RNAi Designer, BLOCK it RNA Express 又は BLOCK it Designer is displayed in new

window and select siRNAs or enter custom siRNA sequence.

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Search siRNA and select Plate in format. Then click Add to Shopping Cart.

Selected siRNAs are displayed in CLIBBOARD field and drag and drop each siRNA to transfer

each wells you hope to input. You can also add Negative control products at the right bottom.

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Click order plate after finished positioning.

After proceeding next step, plate format is not saved. If you plant to reorder, click save plate

and select Yes. Plate format is saved 30days.

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If you need to save format for a long time, click Download Plate and select YES. You can save

plate information as .csv format.

You can import file when you create new file and select Import Plate menu.

Or after open new plate, click Add plate at the top right and add plate format tab.

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Products are added into cart and proceed to ordering step according to Page 15-20.

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8. My Account

During log into, Users can access to order history in past 6 month and edit personal information

in my account menu.

Move the cart to your name and select menu as below.

アカウント: Go to account top page as below

ご注文: View order history

カート: View carts

お気に入りリスト: View favorite list

カスタム製品: No use for primer order

プロファイル: View and edit customer profile

ログアウト:Logout

My account top page

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Orders

Select “ご注文” in my account menu first.

You see order history data during past 6 month. Click each order number to confirm details.

Click 再注文 to order same product again.

Click each menu as below

最近のご注文 Order history

ショッピングカート Cart settings

お気に入りリスト Favorite list

←お客様のアカウント Return to my account top page

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Profile

Select “プロファイル” in my account menu first.

Select menu at the left of my account screen.

プロフィール編集(default) Click “情報を編集” to edit information。

パスワード変更 Change password

秘密の質問 Change secret question

連絡方法 Add email address to send order confirmation email

承認依頼 Ask approval to place order as discussed below

請求先/出荷先住所 Change name of bill to/ship to account

お支払情報 Not in use

←お客様のアカウント Return to my account top page

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Approval routing

If you make primer orders and then ask someone to place order, you have to set approval

settings.

Definition:

Approver: A registered user at your institution to whom your orders will be routed when the

orders exceed the indicated spending limit.

Approvee: A registered user at your institution whose orders you wish to approve.

Search approver

Select ”承認依頼” at the left side. Click ▼ at the right of “注文の承認を依頼する” and then

enter email address in the blank and then click search.

Turn to next screen as below if there is approver email address and then click 変更を保存.

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Notification mail is send to approve(To) and approve(Cc:) as below.

Click ”新しい承認者を追加” if no one approver is found and need to register new approver.

Approver needs to registered customer.

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Enter “姓”,”名”,”E メールアドレス” and 電話番号(optional) and then click 承認者を追加.

Notification mail as below is send to approve(To) and approve(Cc:).

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Approver sets approvee.

Before application of approver from approvee, approver has registered approve. Click ▼ at the

right of “ご注文の承認” and enter approve Email address in blank and click search.

If you search arrpovee email address, you see next screen as below and set spending Limit

(optional). If you have other email address to receive approval information , enter email

address in blank under 追加 E メールアドレス and click 保存. To assign temporary approver,

enter email address and click 検索. Finally click 変更を保存 to change settings.

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9.FAQ

Q1. I can’t login to with my user account and password.

Q2. What characters are used in password?

Q3. What should I enter in order number field?

Q4. Can you receive RNA at home?

Q5. I want to change my setting information.

Q6. I want to order single strand RNA.

Q7. I want to ask other person to receive primers.

Q1. I can’t login to with my user account and password.

A1. We recommend deleting Cookies in web browser first. Delete Cookie according to

guidance. If you don’t solve the issue, contact primer order support team.

℡:03-6832-6980 (Guidance 1 to 2)

Email: [email protected]

How to delete cookies

Internet explorer

Select “ツール”-“インターネットオプション”

Select “削除” in “全般” Tab

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Click ファイルの削除, Cookieの削除 and 履歴の削除.

Google Chrome

Click setting icon at the top right.

Select “設定”.

Click “詳細設定を表示” at the bottom.

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Click “閲覧履歴データの消去”.

Safari (Macintosh)

Click Safari – “キャッシュを空にする”

Click “空にする”

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Firefox

Select “ツール” – “最近の履歴を消去”.

Select “すべての履歴” from pull-down menu and click “今すぐ消去”.

Q2. What characters are used in password?

A2. You need to set password the combination of alphabet and numbers.

Example: password1→OK , password→NG

Q3. What should I enter in order number field?

A3. Any word is OK. This field is required field and enter dot(.) , order date(0120401) and so on.

Q4. Can you receive RNA at home?

A4.We can ship primers only to your laboratory and office.

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Q5. I want to change my setting information.

A5. If you want to change shipping address, you can change shipping address. Click “編集” at

the bottom right in “規定の出荷先住所” as below.

You can’t change dealer information from web. If you need to change dealer, contact us on

page 1.

Q6. I want to order single strand RNA.

A6. Not available for single strand RNA order.

Q7. I want to ask other person to receive primers.

A7. Delivery company rep. has to hand up primer for researcher who placed order directly. If

it’s Ok that anyone at same laboratory can receive primers, enter “どなたでも受け取り可能” in

“受け取り人様氏名(宛名)” field.