Invicta eshre-poster-frequency of aneuploidy

Genetic Laboratory

www.invictagenetics.com

The frequency of aneuploidy status of Day 5 and Day 6 blastocysts assessed by next-generation sequencing technology (NGS) application.Jonna Liss1,2, Anna Bruszczynska1, Sebastian Pukszta1, Krzysztof Łukaszuk1,2,3

1INVICTA, Fertility and Reproductive Centre, Gdansk, Poland2 INVICTA, Fertility and Reproductive Centre, Warsaw, Poland 3 Department of Nursing, Medical University, Gdansk, Poland

Study questionTo assess correlation between Day 5 (D5) and Day 6 (D6) blastocyst morphology and ploidy status.

Genetic Laboratory


Summary answerSlower developing blastocysts cryopreserved on Day 6 but at the same stage of development as those developing to the blastocyst stage on Day 5 do not have similar chromosomal status and therefore provide a lower chance of achieving pregnancy.

What is known alreadyEmbryos with the highest morphological scores showed a higher euploidy rate when compared with lower quality embryos. During IVF cycles, the embryonic cohort is asynchronous in development and trophectoderm biopsy can equally be performed on D5 or D6 post-fertilization and on blastocysts of different morphological quality. It is still unknown whether blastocyst morphology and developmental rate relate to the embryo chromosomal constitution.

Genetic Laboratory


Study design, size, durationRetrospective study including the data analysis of 61 blastocysts stage PGS cycles (median age 38 years, range 34-40) performed between August 2013 and November 2014 at INVICTA Fertility Centre, Poland. A total of 168 blastocysts were evaluated with the NGS protocol.

Participants/materials, setting, methodsAll embryos were cultured to blastocyst stage. Trophectoderm biopsy was performed on D5 (n=104) of development or, for slower growing embryos, on D6 (n=64). The method involved whole genome amplification before NGS protocol. Ion Torrent Suite Software and Invicta Bioinformatics Team Script were used for chromosome copy number variation analysis.

Genetic Laboratory


Main results and the role of chance50 single frozen embryo transfers of euploid blastocysts (D5-39, D6-11) were performed.

Genetic Laboratory


Genetic Laboratory


INVICTA Genetic Laboratoryul. Trzy Lipy 3, 80-172 Gdansk, Poland, T: +48 58 58 58 804, E: [email protected]; W: www.invictagenetics.com

About INVICTA Genetic LaboratoryINVICTA is an experienced genetics laboratory (since 2000)

offering wide range Preimplantation Genetic Diagnosis testing using state of art Next Generation Sequencing (NGS) techniques.

Wider implications of the findings This study provides insight into determining ploidy status depending on the day of blastocyst biopsy. Slower developing blastocysts cryopreserved on Day 6 but at the same stage of development as those developing to the blastocyst stage on Day 5 do not have similar chromosomal status. Euploid embryos tend to show faster progression to the most advanced expansion stages when compared with aneuploid embryos.

Invicta eshre-poster-frequency of aneuploidy

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