Invader assay
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Transcript of Invader assay
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Invader Assay
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We have got the map (genomic sequence) to find treasure so that we can get
treasure chest.
Key to open the chest(Post genomic
technology)
Getting treasure (new drugs)!!
Even if we get the treasure chest (target gene), we can’t open it (because we can’t access to its function
in disease.)
Current genomic researches have tried pulling out of all nails on the chest.However, the number of the nails may be infiinite…
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What is the difference between human & ape? Genomic research
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Progress of Researchgenome Genomic sequence ・ Polymorphism
( SNP etc (
Transcriptome Gene transcription profile
Proteome Expression profile of proteins
Functional proteomeMetabolome
Genetic functionPost translational modification
Protein interation etc.Time consuming and
enormous costElucidation of functional network of cellular molecules
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Technologies in each categories
Genome
Structure analysis(sequence)
Polymorphism analysis
-DNA chip -Invader assay-Sniper assay -PROBE assay
-Luminex -PCR-SSCPPCR-RFLP etc-
Transcriptome・ Differential expression analysis
・cDNA chip etc
ProteomeIdentification
Protein function Protein interactionLigand interaction
Post translational modification
・protein chip, peptide chip・Y2H・SELEX・Phage display・STABLE assay etc
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Used for
The detection of single nucleotide polymorphisms (SNPs)
Detection of mutations that confer resistance to antiviral agents
Genotyping of isolates for epidemiological purposes; categorizes patient isolates into 8 different HBV genotypes (A to H) and 6 different HCV genotypes (1 to 6 with 24 subtypes)
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T
N
C
G A
Reporter probes Flap FlapInveder probesN
C T
GN N
A
Cleavage Cleavage
Endoflap Nuclease
Fluorophore 1 Fluorophore 2Quencher Quencher
C
Cleavage
T
Invader Assay
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Advantage: PCR is unnecessary
Drawback: Quite large amount of sample is required
Background reaction exists