Introduction Bacteria show an incredible diversity with regards to their use of different energy...
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Transcript of Introduction Bacteria show an incredible diversity with regards to their use of different energy...
Introduction
Bacteria show an incredible diversity with regards to their use of different energy sources.
An overview of a hypothetical bacterial cell:
Substrates
Energy conservation (ATP and transmembrane potential)
Biosynthesis
Transport
Movement
Cell division
Energy and Carbon Metabolism: An overview
Chemotrophs Phototrophs
Chemo-lithotrophsChemo-organotrophs
Energy
Carbon metabolism
Heterotroph
Autotroph (CO2)
Basic principles
Mechanisms for the generation of ATP
Substrate level phosphorylation
Respiration
Photophosphorylation
The common denominators in energy metabolism are ATP and transmembrane potentials
Oxidation / Reduction (Redox) reactions
Redox reactions:
Oxidation is the loss of electrons and reduction is the gain of electrons. Electrons cannot exist in solution and the loss of electrons must be coupled to the gain of electrons.
Reduction potential:
This is a quantitqtive measure of the tendency for a substance to give up electrons in biological systems. It is measured in volts and generally at pH = 7.0.
Half reactions
Half reaction are a convenient way of showing the reduced and oxidized form of a compound. Two half reactions are coupled to give a redox reaction.
Half reactions Table 8.1
The number of electrons transferred (n), and the electrode potential under standard conditions (E0') compared to the hydrogen half cell.
Relationship between free energy and reduction potential
Go = -nF Eh
Go = Change in Free energy
n = number of electrons in reaction
F = Faradays constant
Eh = E’ (oxidized) - E’ (reduced)
ATP hydrolysis releases –31.8 kJ / mole so we need at least this amount of energy to make a phosphodiesterase bond in ATP.
Respiration and fermentation
Fermentation: Energy generation by anaerobic energy-yielding reactions characterized by substate level phosphorylation and the absence of cytochrome-mediated electron transfer.
Respiration: Energy generation in which molecular oxygen or some other oxidant is the terminal electron acceptor. Among the latter are nitrate, sulfate, carbon dioxide and fumarate.
Energy and Carbon Metabolism: Fermentative organisms
Chemotrophs Phototrophs
Chemo-lithotrophsChemo-organotrophs
Energy
Carbon metabolism
Heterotroph
Autotroph (CO2)
Substrate level phosphorylation
There are three basic reactions. 1 and 2 are found in most aerobic and anaerobic bacteria which grow on sugars. 3 is found mainly in anaerobic fermenting bacteria.
1. 1,3 bis-phosphoglycerate + ADP 3 phosphoglycerate + ATP
2. phosphoenol pyruvate + ADP pyruvate + ATP’
3. acetyl phosphate + AMP acetate + ATP
Acetyl-coenzyme A (acetyl-CoA)
The thioester bond between the β-mercaptoethylamine moiety of CoA and the acetyl groups (~) is an energy-rich bond.
Respiration and fermentation
Fermentation: Energy generation by anaerobic energy-yielding reactions characterized by substate level phosphorylation and the absence of cytochrome-mediated electron transfer.
Respiration: Energy generation in which molecular oxygen or some other oxidant is the terminal electron acceptor. Among the latter are nitrate, sulfate, carbon dioxide and fumarate.
Energy and Carbon Metabolism: respiration
Chemotrophs Phototrophs
Chemo-lithotrophsChemo-organotrophs
Energy
Carbon metabolism
Heterotroph
Autotroph (CO2)
Components of the electron transport chains
Flavo proteins: carry two electrons (e-) and two protons H+)
Iron sulphide proteins: carry one electron (e-)
Quinones: carry two electrons (e-) and two protons H+)
Cytochromes: carry one electron (e-)
Diversity
In this lecture I have covered basic themes but at the begining of the lecture I said something about metabolic diversity.
This diversity is generated from variation in the electron donor (organic (thousands to choose from) or inorganic ( a few to chose from)) terminal electron acceptor organic (fermentation) or inorganic (respiration).
Use of light energy in the phototrophs.