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PoweringOn & Off

NOTE:

If the STAR Dot Matrix Printer is being used, pleaserefer to the STAR Printer User’s Manual for printerconnection and operation.

Powering OnConnect system according to diagram below.

Before performing this procedure, ensure that theReagent Kit is at the same temperature as theInstrument.

• Switch the Instrument and the printer to ON. (TheInstrument is on when the “I” on the rocker switch isdepressed.) The Instrument will perform a system test.Be sure that the printer is ON LINE.

• Clean vials if necessary.

• Load CAL A and Blank vials into the vial block at theback of the Instrument at least 15 minutes prior to use.

Powering Off• Remove all vials from the Instrument.

• Switch the printer to OFF.

• Switch the Instrument to OFF.

• Store all reagents according to storage requirements.

Power switch

Vial block

The color-coded bars within the text throughout this Guide indicate the sections where specific instructions are given.

PCS Quickstart-15A3179_PCS Quickstart 15A3179 Rev 4/3/13 2:01 PM

Always refer to the PCS Procedure Guide for further instruction

PoweringOn & Off


SeikoPrinter

Controls and Indicators:

Paper Feed Switch: Pressing this switch feeds thepaper continuously. Paper feed will only work whenthe OFF LINE indicator illuminates or flashes.

Power Switch: When the switch is in the ON position,power is supplied to the printer and the POWER andON LINE indicators illuminate. When the printer is outof paper, the OFF LINE indicator flashes.



SeikoPrinter

Loading the Printer Paper:

• Turn the power switch to the ON position.

• Open the paper cover located on the top, right side of theprinter.

• Cut the end of the paper roll cleanly and squarely.

• Insert the end of the paper into the inlet at the bottom of thepaper holder until the auto-loader catches it and feeds thepaper through.

• The outside of the roll is the printable surface for thethermal paper roll.

• Close the paper cover.


ConsumableComponents

Vial Cleaning

To ensure accurate results, all vials should be freeof fingerprints and smudges prior to placing in thevial block for equilibration. All vials should becleaned prior to use.

• Holding the vial by the cap, immerse the lowerportion of the vial (about 2.5 cm) into cleanisopropyl alcohol.

• Remove the vial from the alcohol and gentlywipe the lower portion of the vial by rotating thevial against a lint-free wipe.

Disposal

• All PCS reagents are non-hazardous and aqueousbased.

• Small quantities may be disposed of in sewer. Largequantities should be disposed of in accordance withlocal, state or federal regulations.

If Reagent Kit has been moved from another location,depending on the difference in temperature betweenthe two locations, an hour may be required forequilibration.

Removing the kit components from the box andletting them stand in free air will hasten equilibration.

Storage

• The Reagent Kit should be stored at room temperature(18-28° C).

• Avoid temperatures exceeding 32° C.

• Kits should be stored in the dark to the extent practical.

• Frozen reagents should be brought to room temperatureand inverted several times prior to use.



Consumable Components

Reagent Kit

• Blank Reagent Vials:Standard Reagent Kits contain 12 vials pre-filled withBlank Solution.

Bulk Reagent Kits contain 50 vials pre-filled with BlankSolution.

• Sample Solutions:Range 1: For pipette volumes 200 – 5000 µL

Range 2: For pipette volumes 50 – 199 µL



Range 5*: For pipette volumes 0.5 – 1.9 µL

Range 6*: For pipette volumes 0.1 – 0.49 µL

• Sample Aliquot Containers:Use aliquot containers to hold Sample Solutions usedduring calibration to avoid contamination of SampleSolution bottle.

• Transfer Pipettes:Plastic transfer pipettes are used to transfer SampleSolution from the reagent bottle to the aliquot container.

• Calibration Standard:CAL A vial is used to collect a zero reading for theInstrument.

• Kit Inserts:Declaration of Traceability

*Range 5 and 6 are sold separately.Lot code card


PipetteCalibration Setup

Starting a Pipette Calibration

• At the Main Menu prompt (Figure 1), press YES tobegin the pipette calibration procedure. The Instrumentwill perform a temperature check.

• The Instrument will prompt for lot code (Figure 2). TheInstrument retains up to 6 previously entered lot codes.If the entered code matches one previously entered,press NO and re-enter the code.

• Press YES if entering a new lot code. Group numbersfound on the correct side of the lot code card must beentered. Press ENTER after each group of digits isentered.

• A confirmation prompt will be displayed (Figure 3).Press YES.

• The Instrument will perform a brief self-calibration.Immediately following the self-calibration the Instrumentwill prompt to insert the CAL A vial (Figure 4).

• Open the vial holder cover and insert the CAL A vialpressing down until it meets the bottom of the vialholder.

• The Instrument will then perform a zero check andprompt to remove the CAL A when the test is complete.Return the CAL A vial to the Reagent Kit for future use.

Main MenuPipette Calibration?

Figure 1

Enter Reagent Lot Code:

Lot Code XXXXXCorrect? (Y/N)

Figure 2

Figure 3

InsertCal A Vial

Figure 4



Important Notes:• Use the CAL A vial from the Reagent Kit and not from the Instrument Calibrator Kit.

• Never remove the cover of the CAL A vial.

• The label for the CAL A vial should face the front of the Instrument with the vertical line facing forward.

• Each Blank vial contains a predetermined volume of solution. Any spillage will reduce accuracy.

• The lower portion of the Blank vial must be free of smudges. Touching this area should be avoided.

• Once the Blank vial has been inserted into the Instrument, it must not be turned or moved until the calibrationprocedure is complete for that vial.

• The Instrument will then prompt to insert a blue Blankvial into the vial holder (Figure 5). Remove the Blank vialfrom the vial block and remove the cap.

• Insert the open vial into the vial holder pressing downuntil it contacts the bottom of the vial holder.

• Close the Instrument cover. The Instrument will read theBlank vial.

Remove Cap andInsert Blank

Figure 5


• The Instrument will prompt to enter an operator ID(Figure 6). Enter a number (not exceeding 20 digits) toidentify the operator. For alpha character entry, refer toAppendix E of the PCS Procedure Guide.

• Enter the pipette ID (Figure 7). Type in a number notexceeding 20 digits and press ENTER. The printer willprint the calibration header information.

• Enter the volume (in microliters) to be dispensed andpress ENTER (Figure 8). The CLEAR key can be usedto correct any typing errors.

Enter Operator ID:

Enter Pipette ID:

Enter Pipette Volume: ___ µL

Figure 6

Figure 7

Figure 8



Important Notes:• The proper range of Sample Solution for the pipette volume to be tested must be used.

• Each Reagent Kit contains bottles of Sample Solution corresponding to various pipette volume ranges.

• If an improper Sample Solution has been dispensed into the Blank vial, the results obtained will not be valid andthe vial is no longer usable. It must be discarded.

• The number of samples that can be dispensed in thisvial, at the selected volume, will be displayed (Figure 9).

• Press YES and the Instrument will display theappropriate Sample Solution to use for the pipettecalibration.

• The Instrument will take a final reading of the Blank vialand then prompt for the first dispense of the SampleSolution with the pipette under test (Figure 10).

XX Left this VialProceed? (Yes/No)

Range XPipette Sample #1

Figure 9

Figure 10

PipetteCalibration Setup


Aspirating and Dispensing Sample Solution

Using a plastic transfer pipette, transfer an aliquot of SampleSolution into an aliquot container. Both items are located inthe Reagent Kit. Transfer only enough solution for half-hourincrements. The volume gradations on the transfer pipetteand the 1 mL mark on the aliquot container can be used asa guide for the volume of solution transferred.

• Aspirate the Sample Solution from the aliquot containerwith the pipette under test.

• Lift the vial holder cover and dispense the Sample Solutioninto the Blank vial using standard pipetting technique.

Important Note:The Sample Solution should be dispensed against the inside wall of the vial with the pipette tip placed above themeniscus but below the shoulder of the Blank vial. The vortex mixing action will wash the solution from the insidewall of the vial.

PipetteCalibration



Mixing Sample #1Please Wait

Reading Sample #1Please Wait

Result #1 XXX.XPipette Sample #2

Rules for Adjustable Volume Pipettes:• Calibration can be performed by starting at any volume.

• When the desired number of samples at the first volume have been dispensed, press ENTER.

• If a data point must be deleted, press YES at the reprint results prompt and follow the delete a data point instructions.

• Enter the next volume in the pipette’s range. This process can be repeated. Press END OF RUN when complete.

• In some cases, more than one Sample Solution will be required. Special attention should be paid to the screenprompts to ensure usage of correct solution.

Reading the Sample• Once the sample is dispensed, and the cover is closed, the

Instrument will mix the sample and display a prompt (Figure 11).

• The Instrument will then read the absorbance of the sampleand display a prompt (Figure 12).

• Finally, the result of the reading will be displayed on the screenand the Instrument will prompt for the dispense of the nextsample (Figure 13).

• Follow the same steps listed on this page for the next sampleand every subsequent sample.

• When performing a single volume calibration and the desirednumber of data points have been collected, press END OFRUN. When performing a multi-volume calibration, pressENTER (see shaded section below).

• The group statistics will be computed and the results printed.

Figure 11

Figure 12

Figure 13

PipetteCalibration


Reprint Results?(Yes/No)

Delete Data Points?(Yes/No)

Enter Pt # to Delete

• If, at the end of the run, a second copy of the results is desired, press YES at the next prompt (Figure 14).

• If it is determined that a given data point may be eliminated from the statistical calculation, press YES at the next prompt (Figure 15).

• Enter the sample # of the data point to be deleted at the next prompt (Figure 16).

• At the next prompt to delete more data points, follow the same steps until all appropriate data points are deleted.

• The correct statistics will be printed.

• For suggested tolerance limits, refer to section 4.13 of the Procedure Guide.

Figure 14

Figure 15

Figure 16

CompletingCalibration



Another Cal w/Vial?(Yes/No)

Use New Vial?(Yes/No)

End of Vial

• The number of data points that can be collected in each Blankvial is dependent on the sample volume and will range from 1point to 40.

• Each time a pipette calibration run is completed, theInstrument will display the remaining number of samples of theselected volume that may be dispensed into the vial.

• In any one Blank vial, calibrations may be performed at thesame volume, at different volumes with the same SampleSolution or at different volumes with different SampleSolutions.

• To use the same vial after the completion of the firstcalibration, press YES at the first prompt (Figure 17). Thecalibration cycle will restart.

• When the maximum capacity of the vial has been reached,alternating prompts will flash (Figures 18 & 19).

• If more data points are required, answer YES to the Use NewVial prompt. The Instrument will prompt to remove the old vialand insert a new one. This process may be repeated ifneeded.

Figure 17

Figure 18

Figure 19

CompletingCalibration


InstrumentCalibration

Main MenuInstr. Calibration?

Instrument Cal. MenuPerform Instr. Cal.?

Calibrating the PCS:

• To access the Instrument Calibration Menu, press MENUSCROLL until the Instrument Calibration prompt is displayed(Figure 20).

• Press YES at the prompt.

• The Instrument Calibration Menu will open.

• Press YES at the Perform Instrument Calibration prompt(Figure 21).

Important Notes:• Minimally, the Instrument Calibration should be performed every 30 days or prior to the next use, whichever is

later.• The Instrument is calibrated using the PCS Instrument Calibrator Kit, which contains 4 vials with calibrator

values: CAL A, B, C and D. • The contents of the kit are subject to degradation with time, temperature and exposure to light.

Figure 20

Figure 21



InstrumentCalibration

CAL Lot Code XXXXXNew Lot? (Y/N)

Insert CAL A Vial

Insert CAL B Vial

• At the prompt, enter the Operator ID for the Instrumentcalibration. For alpha character entry, see appendix E of theProcedure Guide.

• The Instrument will prompt to enter the Calibrator Kit lot code.This code is a four or five digit number found on the insidelabel of the Instrument Calibrator Kit box lid.

• After entering the lot code, press ENTER. If this lot codematches one previously entered, the next prompt will be toinsert the first Cal Vial.

• If the lot code is for a new kit, press YES when the Instrumentidentifies it as new (Figure 22).

• The new calibrator values are found on the box label and mustbe entered at the prompt.

• The leading zeros and the decimal points must be entered.Press YES to confirm when the lot code is completely entered.

• After the lot code is successfully entered, the Instrument willtake a series of readings and then prompt for the first calibratorvial (Figure 23).

• Use the CAL A vial in the Instrument Calibrator Kit.

• When the readings are complete, the Instrument will thenprompt for the next vial (Figure 24).

• The remainder of the calibrator vials must be inserted at thesequential prompts. Results will be printed at conclusion.

• The entire process takes approximately 12 minutes tocomplete.

Important Notes:• If the calibration is successful, the date of the last

Instrument calibration check will change to the currentday.

• Never remove the caps of the calibrator vials.

• Printed results will include:

- Reading data- Instrument accuracy data- Instrument repeatability data- Overall calibration result

Figure 22

Figure 23

Figure 24


15A69

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