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  • Seediscussions,stats,andauthorprofilesforthispublicationat:https://www.researchgate.net/publication/313265127

    Injectableplateletrichfibrin(i-PRF):opportunitiesinregenerativedentistry?

    ArticleinClinicalOralInvestigationsFebruary2017

    DOI:10.1007/s00784-017-2063-9

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    7authors,including:

    Someoftheauthorsofthispublicationarealsoworkingontheserelatedprojects:

    InvestigatetheeffectoflowercentrifugationspeedandtimeonPlateletRichFibrin(PRF)

    formulationsleadingtotheiradvancedprotocols:A-PRF+andi-PRFViewproject

    Anti-bacterialnanostructuresandbiomimeticmaterialsViewproject

    RichardJMiron

    NovaSoutheasternUniversity

    105PUBLICATIONS1,026CITATIONS

    SEEPROFILE

    YufengZhang

    WuhanUniversity

    108PUBLICATIONS1,703CITATIONS

    SEEPROFILE

    ShahramGhanaati

    GoetheUniversityofFrankfurt/Main;Universi

    99PUBLICATIONS1,108CITATIONS

    SEEPROFILE

    JosephChoukroun

    Goethe-UniversittFrankfurtamMain

    39PUBLICATIONS1,728CITATIONS

    SEEPROFILE

    AllcontentfollowingthispagewasuploadedbyRichardJMironon07February2017.

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    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  • ORIGINAL ARTICLE

    Injectable platelet rich fibrin (i-PRF): opportunitiesin regenerative dentistry?

    Richard J. Miron1,2,3 & Masako Fujioka-Kobayashi1,4,5 & Maria Hernandez1 &Umadevi Kandalam6 & Yufeng Zhang7 & Shahram Ghanaati8 & Joseph Choukroun9

    Received: 31 August 2016 /Accepted: 25 January 2017# Springer-Verlag Berlin Heidelberg 2017

    AbstractObjectives Platelet rich plasma (PRP) has been utilized inregenerative dentistry as a supra-physiological concentrate ofautologous growth factors capable of stimulating tissue regen-eration. Despite this, concerns have been expressed regardingthe use of anti-coagulants, agents known to inhibit woundhealing. In this study, a liquid formulation of platelet richfibrin (PRF) termed injectable-PRF (i-PRF) without the useof anti-coagulants was investigated.Materials and methods Standard PRP and i-PRF (centrifugedat 700 rpm (60G) for 3 min) were compared for growth factorrelease up to 10 days (8 donor samples). Furthermore, fibro-blast biocompatibility at 24 h (live/dead assay); migration at24 h; proliferation at 1, 3, and 5 days, and expression of

    PDGF, TGF-, and collagen1 at 3 and 7 days wereinvestigated.Results Growth factor release demonstrated that in generalPRP had higher early release of growth factors whereas i-PRF showed significantly higher levels of total long-term re-lease of PDGF-AA, PDGF-AB, EGF, and IGF-1 after 10 days.PRP showed higher levels of TGF-1 and VEGF at 10 days.While both formulations exhibited high biocompatibility andhigher fibroblast migration and proliferation when comparedto control tissue-culture plastic, i-PRF induced significantlyhighest migration whereas PRP demonstrated significantlyhighest cellular proliferation. Furthermore, i-PRF showed sig-nificantly highest mRNA levels of TGF- at 7 days, PDGF at3 days, and collagen1 expression at both 3 and 7 days whencompared to PRP.Conclusions i-PRF demonstrated the ability to release higherconcentrations of various growth factors and induced higherfibroblast migration and expression of PDGF, TGF-, andcollagen1. Future animal research is now necessary to furthervalidate the use of i-PRF as a bioactive agent capable of stim-ulating tissue regeneration.Clinical relevance The findings from the present study dem-onstrate that a potent formulation of liquid platelet concen-trates could be obtained without use of anti-coagulants.

    Keywords Fibrin . Blood platelets . Regeneration .Woundhealing . Fibroblasts . Platelet rich fibrin

    Introduction

    Platelet concentrations have been utilized in dentistry for overthree decades as a regenerative tool capable of releasing supra-physiological doses of growth factors responsible for inducingtissue regeneration derived from autologous sources [1, 2].

    Richard J. Miron and Masako Fujioka-Kobayashi contributed equally tothis work.

    * Richard J. Mironrmiron@nova.edu

    1 Department of Periodontology, College of Dental Medicine, NovaSoutheastern University, Fort Lauderdale, FL, USA

    2 Cell Therapy Institute, Center for Collaborative Research, NovaSoutheastern University, Fort Lauderdale, FL, USA

    3 Department of Periodontics and Oral Medicine, University ofMichigan, Ann Arbor, MI, USA

    4 Department of Cranio-Maxillofacial Surgery, University of Bern,Bern, Sw