Influences of duodenal vivonex and ensure infusion on ileal and colonic water electrolyte absorption
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I'31INFLUENCES OF DUODENAL VIVONEX AND ENSURE INFUSION ON ILEAL AND CO- LONIC WATER ELECTROLYTE ABSORPTION: B.E.Brown, D.B.A.Silk. Depart- ment of Gastroenterology and Nutrition, London, NW10 tngland. Duodenal infusion of a simulated nutrient mixturi has been shown to cause a distal intestinal secretion of fluid and electro- lytes in man (Gastroenterology E,94,1979): The present study was carried out to determine whether similar mechanisms can be impli- cated in the pathogenesis of enteral feeding related diarrhoea. An invivo segmental perfusion technique has been used in rats to inves tigate the influence of duodenal infusion of undiluted elemental (Vivonex) and polymeric (Ensure)diets on simultaneous ileal and colonic water and electrol tonic saline (0.2 ml -+i te handling. Duodenal infusion of iso- min was associated with net absorption of water and electrolytes from a 25 mM bicarbonate-saline solution (PH 7.4) perfused (0.2 ml min-l) in the ileum or colon. Duodenal infusion of Vivonex was associated with increases of 44.7 + SE 11.2% and 13.0 t 4.1% in ileal water and Na+ absorption and 169.7 + 25.3 and 27.3 + 9.2% increases in colonic water and Nat absorp- tion. Ensure perfusion was associated with increases of 65.8 + 18.7% and 154;4 + 40.7% in ileal water and Na+ absorption, an-in- crease of 13.8 + 4.2% in colonic water absorption and a decrease of 19.5 + 6.8% in sodium absorption. None of these differences was statistically significant. The proximal infusion of an ele- mental or polymeric diet therefore has no adverse effect on ileal or colonic water and electrolyte absorption, which makes it un- likely that enteral feeding related diarrhoea is caused by a hormo- nally mediated mechanism. P32 RESPONSES OF RAT INTESTINAL MUCOSA, EPITHELIAL CELL RENEWAL AND PLASMA LIPOPROTEIN TO INTRAVENOUS FEEDING: M.Kawamura. S.Kimura. Laboratory of Nutrition of Agriculture, Tohoku University, Sen- dai, Japan. Little is known how the presence of food in the intestinal tract influences the structural and functional integrity of the small intestine. Although the morphologic or functional events of changes in the intestinal tract have been well defined in the absence of food, it has been impossible to isolate the effects of the absence of food from the‘effects of starvation, and less is known concerning the mechanism by which the effects of oral intake behaviour is mediated. The present investigation was per- formed in order to verify the above problem using intravenous- fed rat. In the previous study it was found that the shape of jejunum villi in rats fed intravenously differed from that of rats fed orally or fasted.In this study, the relationship bet- ween the morphological state and physiological changes in the small intestine of rats fed orally, intravenously or fasted were determined. As a result, mucosal wet and dry weight, and mucosal protein content were highest when the diet was given orally, intermediate when given intravenously, and lowest when fasting. The DNA content of mucosa was significantly lower following in- travenous feeding or fasting than after oral feeding. There was no sienificant difference between intravenous feedina and fas- ting.-The composition of mucosal protein following intravenous feeding differed from that following oral feeding or fasting. The migration of jejunum epithelial cells was prolonged following intravenous feeding compared with that after oral feeding. In- travenous feeding greatly decreased plasma HDL-cholesterol and mucosal HDL apolipoprotein (apo A-I). 122
Transcript of Influences of duodenal vivonex and ensure infusion on ileal and colonic water electrolyte absorption
I'31 INFLUENCES OF DUODENAL VIVONEX AND ENSURE INFUSION ON ILEAL AND CO- LONIC WATER ELECTROLYTE ABSORPTION: B.E.Brown, D.B.A.Silk. Depart- ment of Gastroenterology and Nutrition, London, NW10 tngland.
Duodenal infusion of a simulated nutrient mixturi has been shown to cause a distal intestinal secretion of fluid and electro- lytes in man (Gastroenterology E,94,1979): The present study was carried out to determine whether similar mechanisms can be impli- cated in the pathogenesis of enteral feeding related diarrhoea. An invivo segmental perfusion technique has been used in rats to inves tigate the influence of duodenal infusion of undiluted elemental (Vivonex) and polymeric (Ensure)diets on simultaneous ileal and colonic water and electrol tonic saline (0.2 ml -+i
te handling. Duodenal infusion of iso- min was associated with net absorption of
water and electrolytes from a 25 mM bicarbonate-saline solution (PH 7.4) perfused (0.2 ml min-l) in the ileum or colon. Duodenal infusion of Vivonex was associated with increases of 44.7 + SE 11.2% and 13.0 t 4.1% in ileal water and Na+ absorption and 169.7 + 25.3 and 27.3 + 9.2% increases in colonic water and Nat absorp- tion. Ensure perfusion was associated with increases of 65.8 + 18.7% and 154;4 + 40.7% in ileal water and Na+ absorption, an-in- crease of 13.8 + 4.2% in colonic water absorption and a decrease of 19.5 + 6.8% in sodium absorption. None of these differences was statistically significant. The proximal infusion of an ele- mental or polymeric diet therefore has no adverse effect on ileal or colonic water and electrolyte absorption, which makes it un- likely that enteral feeding related diarrhoea is caused by a hormo- nally mediated mechanism.
P32 RESPONSES OF RAT INTESTINAL MUCOSA, EPITHELIAL CELL RENEWAL AND PLASMA LIPOPROTEIN TO INTRAVENOUS FEEDING: M.Kawamura. S.Kimura. Laboratory of Nutrition of Agriculture, Tohoku University, Sen- dai, Japan.
Little is known how the presence of food in the intestinal tract influences the structural and functional integrity of the small intestine. Although the morphologic or functional events of changes in the intestinal tract have been well defined in the absence of food, it has been impossible to isolate the effects of the absence of food from the‘effects of starvation, and less is known concerning the mechanism by which the effects of oral intake behaviour is mediated. The present investigation was per- formed in order to verify the above problem using intravenous- fed rat. In the previous study it was found that the shape of jejunum villi in rats fed intravenously differed from that of rats fed orally or fasted.In this study, the relationship bet- ween the morphological state and physiological changes in the small intestine of rats fed orally, intravenously or fasted were determined. As a result, mucosal wet and dry weight, and mucosal protein content were highest when the diet was given orally, intermediate when given intravenously, and lowest when fasting. The DNA content of mucosa was significantly lower following in- travenous feeding or fasting than after oral feeding. There was no sienificant difference between intravenous feedina and fas- ting.-The composition of mucosal protein following intravenous feeding differed from that following oral feeding or fasting. The migration of jejunum epithelial cells was prolonged following intravenous feeding compared with that after oral feeding. In- travenous feeding greatly decreased plasma HDL-cholesterol and mucosal HDL apolipoprotein (apo A-I).
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