INERTSIL (GLSciences) - Canadian Life · PDF fileINERTSIL ® (GLSciences) ... columns...

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INERTSIL® (GL Sciences)

Inertsil®Manufactured by GL Sciences, Tokyo, Japan, Inertsil® LCcolumns represent state-of-the-art silica manufacturingtechnology and are unrivaled for their physical and chemicalstability.

Inertsil® silica gels are free of dents and cracks, which mightweaken the spherical particles or lead to inefficient columnpacking. The uniformity of the base silica, with a purity of99.999%, greatly contributes to the chemical stability andexcellent resistance to high and low pHs.

SEM photographs of Inertsil® silica gels are shown below.There are many silica gels on the market which have rough sur-faces and cracks.In contrast, Inertsil® silica gels have a highly smooth surface andspherical shape, which provides durability and high inertnesswith good reproducibility over a long period of time, making themideal for LC applications.A rigorous test by SEM is employed for base silica gels forInertsil® packings.

What makes the column back pressure different?Size of silica gel, uniformity of the particle size and particle shape

SEM photograph - Inertsil® Silica Gel

Why is the particle size distribution different?Every commercially available column has a particle size distribution, but its distribution widths can be quite different.

Particle size, surface area, pore diameter and pore volume arecontrolled by using nitrogen adsorption and laser ray particleanalyzer.

Packings with an uniform size and smooth surfaceshow very low column back pressure.

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Inertsil® 4 Series

Inertsil® Series 4 phases

The Inertsil® 4 series is GL Sciencesʼ latest generation of LC columns. This newly developed chemical bonding technology allowsInertsil® ODS-4 to provide high efficiency and good peak shape where competitive columns fail.

Phase Endcapped Particle Size (µm) Pore Size (Å)Surface Area

(m2/g)Carbon Load

(%)

ODS-4 Yes 2, 3, 5 100 450 11C8-4 Yes 2, 3, 5 100 450 5

Inertsil® ODS-4• Improved peak shapes and heights• Enhanced sensitivity• High resolution• Fast equilibration• Compatible with 100% aqueous eluents

Ultimate deactivation techniques enable the highest inertnesswith this silica based LC column, allowing analysis of traceamounts of the most demanding basic and acidic compounds.

Strictly classified particle size with narrow distribution width andideally designed carbon loading and its procedure allows GLSciences to guarantee the high plate count of +100,000/m.

Combined with the synergistic effect from the improved peakshape based on the high inertness of the packing material,Inertsil® ODS-4 can be used as a tool for enhancing sensitivityand high separation efficiency.

Excellent peak shapes for both strong acidic andbasic compounds

GL Sciencesʼ newly developed endcapping technique ideallydeactivates the silanol groups, suppressing the adsorption ofcommon basic compounds as well as strong ones, which leadsto sharper peaks.

The new endcapping technique prevents the adsorption of strongacidic compounds as the surface of the packing materialbecomes neutral.

Haloacetic acid is a harmful substance generated when tap wateris chlorinated. With its strong acidity, it is easily adsorbed atbasic sites on the surface of the packing material.

High-sensitivity analysis by LC-MS/MS has become increasinglypopular. A column free of adsorption is required since the adsorp-tion of even a very small amount can adversely affect the preci-sion of such highly sensitive quantitative analysis.

Inertsil® ODS-4 is ideal for LC-MS/MS analysis of strong acids(see Figure 1).

Antihistamines are strongly basic and therefore will easily beadsorbed to the residual silanols on the packing material, result-ing in deterioration of peak shapes.

Generally, in LC analysis of strong basic compounds, highly-con-centrated buffer or ion-pair reagents are introduced to the eluentto prevent adsorption of strong basic samples on the packingmaterial. However, in LC-MS/MS, such eluent conditions are notrecommended and a packing material with superb inertness isrequired (see Figure 2).

Inertsil® ODS-4 prevents the adsorption of strong basic com-pounds without using the above mentioned eluents. This featuremakes Inertsil® ODS-4 an ideal column for LC-MS/MS.

System : LC800 LC system Detection: LC-MS/MS(ESI,Negative,MRM)4000 Q TRAP® Injection Vol. : 10 µL

Column : Inertsil ODS-4 3µm, 150mm × 2.1mmEluent : A) CH3OH

B) 0.1% HCOOH A/B = 5/95 - 2.5 min- 5/95 - 2.5 min ‒ 95/5 - 5 min- 95/5Flow Rate : 0.2 mL/minCol. Temp. : 40°C

Sample:1. Bromoacetic acid2. Dibromoacetic acid3. Tribromoacetic acid4. Chloroacetic acid5. Dichloroacetic acid6. Trichloroacetic acid7. Bromochloroacetic acid

Figure 1. Analysis of haloacetic acids by LC-MS/MS



Inertsil® ODS-4 (cont’d)

Generally, in LC analysis of strong basic compounds, highly-con-centrated buffer or ion-pair reagents are introduced to the eluentto prevent adsorption of strong basic samples on the packingmaterial. However, in LC-MS/MS, such eluent conditions are notrecommended and a packing material with superb inertness isrequired (see Figure 2).

Inertsil® ODS-4 prevents the adsorption of strong basic com-pounds without using the above mentioned eluents. This featuremakes Inertsil® ODS-4 an ideal column for LC-MS/MS.

Excellent analysis stability of strong metalchelating compounds

For stable analysis of strong metal chelating compounds, sam-ples were injected repeatedly before the analysis to mask theadsorption active sites on the surface of the packing material oraddition of EDTA to the eluent were required.

Column equilibration was also time consuming.Since the trace amount of metals has been eliminated from thesurface of the packing material, it allows a stable analysis fromthe 1st injection requiring no masking process (see Figure 3).

Tetracyclines are antibiotics broadly used as pharmaceuticals foranimals. The residues in livestock and sea product are a majorissue today.

See Figure 5 (next page) for an inertness comparison onHinokitiol.

High-sensitivity analysis by LC-MS/MS has become a large fieldof interest. As tetracyclines have a very strong chelating charac-teristic, it has been anticipated that the adsorption on the columndeteriorates quantitative precision.

Inertsil ODS-4 is an ideal column for LC-MS/MS analysis ofchelating compounds since it has completely removed metalsfrom the surface of the packing material.

Excellent analysis stability under 100% aqueouseluents

Under the condition of a mobile phase containing very littleorganic solvent, the mobile phase can easily come out frompores in the packing material, resulting in the dewettingphenomenon. As shown in Figure 4 (next page), such waterrepellency makes the analysis results unstable over the reten-tion time. Inertsil® ODS-4, with an ideal chemical processing,minimizes such dewetting phenomenon, achieving reliableelution with 100% aqueous eluents

Inertsil® ODS-4 minimizes the dewetting phenomenon whilemaintaining the same retention characteristics to that of com-mon ODS columns. Therefore, Inertsil ODS-4 can be used atease under 100% aqueous eluents.

Packing material that achieves a stable analysis using 100%aqueous eluents provides another advantage. There isreduced equilibration time returning to an initial eluentcondition with a gradient separation.

System : LC800 LC system Detection: LC-MS/MS ESI,Positive,MRM4000 Q TRAP® Injection Vol. : 10µL

Column : Inertsil ODS-4 3µm, 150mm × 2.1mmEluent : A) CH3OH B) 2 mM CH3COONH4 A/B = 40/60 - 10 min- 95/5 - 5 min - 95/5Flow Rate : 0.2 mL/minCol. Temp. : 40°C

Sample:1. Chlorpheniramine 0.1 mg/L2. Cinnarizin 0.1 mg/L3. Clemastine 0.1 mg/L4. Difenidol 0.1 mg/L5. Diphenhydramine 0.1 mg/L6. Diphenylpyraline 0.1 mg/L7. Hydroxyzine 0.1 mg/L8. Promethazine 0.1 mg/L9. Triprolidine 0.1 mg/L

Figure 2. Analysis of antihistamines by LC-MS/MS

System : LC800 LC system Detection: LC-MS/MS(ESI,Positive,MRM)4000 Q TRAP® Injection Vol. : 10 µL

Column : Inertsil® ODS-4 3µm, 75mm × 2.1mmEluent : A) 0.1% CH3OH

B) 0.1% HCOOH A/B = 20/80 - 4.5 min- 20/80 - 2 min ‒ 95/5 - 3.5 min- 95/5Flow Rate : 0.2 mL/minCol. Temp. : 40°C

Sample:1. Tetracycline (TC) (100µg/L)2. Oxytetracycline (OTC) (100µg/L)3. Chloretracycline (CTC) (100µg/L)4. Doxycycline (DC) (100µg/L)

Figure 3. Analysis of tetracyclines by LC-MS/MS



Inertsil® ODS-4 (cont’d)

Testing procedureIntroduce 100% water for 20 minutes

Analysis (Upper chromatograms)

Stop flow for 15 minutes

Introduce eluent for 30 minutes

Stop flow for 15 minutes

Introduce eluent for 15 minutes

Analysis (Lower chromatograms)

System : GL-7400 LC systemDetection: UV 254 nmColumn : Inertsil® ODS-4 5µm, 250mm × 4.6mm

Eluent : H2OFlow Rate : 1.0 mL/minCol. Temp. : 40°C

Sample:1. Cytosine2. Uracil3. Guanine4. Thymine5. Adenine

Figure 4. Comparison of analyses under 100% aqueous eluents

Inertsil® ODS-4 Symmetry® C18 SunFire™ C18

Figure 5. Comparison of inertness to Hinokitiol between Inertsil® ODS-4 and commercially available ODS columns



Inertsil® C8-4It is ideal to use an Octyl group bonded column for the analysisof samples containing hydrophobic compounds as the analysistime can be shortened.

Inertsil® C8-4 provides the same separation pattern and extremeinertness to any type of compound as the Inertsil® ODS-4 includ-ing the high stability to 100% aqueous mobile conditions.

System : GL7400 LC systemColumn : Inertsil® C8-4 5µm, 150mm × 4.6mmEluent : A) CH3OH

B) H2O(A/B) = (80/20)

Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV 254 nM

Sample:1. Uracil2. Caffeine3. Phenol4. n-Butylbenzene5. o-Terphenyl6. n-Amylbenzene7. Triphenylene

Figure 1. Comparison of retention and separation pattern

Column : Inertsil® C8-4 5µm, 150mm × 4.6mmEluent : A) CH3CN

B) 25mM phosphate buffer, pH 7.0(A/B) = (40/60)

Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV 220 nM

Sample:1.0 µL (0.1mg/mL)1. Phenol2. Dextrometrophan

hydrobromide

Figure 2. Adsorption performance on various compoundsColumn : Inertsil® C8-4 5µm, 150mm × 4.6mmEluent : A) CH3CN

B) 0.1% H3PO4 Sample: 3.0 µL(A/B) = (25/75) 1.Brilliant Blue FCF

Flow Rate : 1.0 mL/min (0.05mg/mL)Col. Temp. : 40°C 2. Phenol (0.3mg/mL)Detection: UV 254 nM 3. Salicylic acid (0.2mg/mL)

Column : Inertsil® C8-4 5µm, 150mm × 4.6mmEluent : A)CH3CN

B) 0.1% H3PO4 (A/B) = (40/60)Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV 254 nM

Sample:1.0 µL (0.1mg/mL)1. β - Thujaplicin

(Hinokitiol)

System : GL7400 LC systemColumn : Inertsil® C8-4 5µm, 150mm × 4.6mmEluent : 100% H2OFlow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV 254 nM

Sample:1. Cytosine2. Urasil3. Guanine4. Thymine5. Adenine

Figure 3. Confirming the stability to 100% aqueous mobile phase

As Inertsil® C8-4 was designed to minimize the dewettingphenomenon, it provides superb stability and reproducibility evento those critical water rich mobile phase conditions.As a result, rapid analysis can be achieved in a gradient modewith the equilibration time of the column being short.



• Maintain high efficiency over a wide range offlow rates

• Symmetric peaks for basic, acidic andchelating compounds

• Column lengths up to 150mm operating up to80MPa (800bar)

• 2µm shows exact same selectivity as largerparticles

Inertsil® ODS-4 columns have proven excellent for analysis ofpharmaceutical bases, acids, chelating compounds andzwitterions.

Analysts currently using Inertsil® ODS-4 3µm or 5µm in theirmethods can now achieve ultra high speed UHPLC separationsas well.

Column lengths of 30mm to 150mm are available to accomodateany range of sample complexity.

Inertsil® ODS-4 2µm

Figure 1.

Figure 2.

INERTSIL® 2µm UHPLC Columns



Figure 3.

Inertsil® ODS-4 2µm (continued)

Description Maximum Operating Pressure(MPa)

Length(mm)

ID 2.1mm ID 3.0mm

Inertsil® ODS-42µm

80 Mpa (800bar)(~12,000 psi)

30 IN5020-81200 IN5020-8121050 IN5020-81202 IN5020-8121275 IN5020-81203 IN5020-81213100 IN5020-81204 IN5020-81214150 IN5020-81205 IN5020-81215

Inertsil® C8-42µm

30 IN5020-81280 IN5020-8129050 IN5020-81282 IN5020-8129275 IN5020-81283 IN5020-81293100 IN5020-81284 IN5020-81294150 IN5020-81285 IN5020-81295

Inertsil® 2µm Analytical Ordering Guide

Description Maximum Operating Pressure(MPa)

Length(mm)

ID 2.1mm ID 3.0mm

Inertsil® ODS-32µm

80 Mpa (800bar)(~12,000 psi)

75 IN5020-84653 IN5020-84663100 IN5020-84654 IN5020-84664150 IN5020-84655 IN5020-84665

Inertsil® C8-32µm

75 IN5020-84932 IN5020-84937100 IN5020-84933 IN5020-84938150 IN5020-84934 IN5020-84939

Inertsil® Ph-32µm

75 IN5020-85132 IN5020-85137100 IN5020-85133 IN5020-85138150 IN5020-85134 IN5020-85139

Convert your ODS-3 to UHPLC, now also available in 2µm (see page 142).

Please see pg. 154-161 for more ordering and pricing information



InertSustain™ C18Introducing a radically new type of silica

It was not possible to end-cap 100% of residual silanols using tra-ditional chemical modification procedures.GL Sciences studied the possibility of developing a radically newtype of silica, a silica that would provide both high intertness(base deactivation) and durability at a wide range of pH.

Our focused R&D effort lead to the development of “EvolvedSurface Silica”, in which adsorption sites and silanol distributionare strictly controlled.

This process enables delivery of an HPLC column with the mostadvanced combination of base deactivation and chemical com-patibility (see Figure 1).

The introduction of Evolved Surface Silica and our cutting-edgechemical bonding technology make InertSustain™ C18 compati-ble with100 % aqueous mobile phases, while maintaining strongnon-polar retentivity. InertSustain™ C18 offers superb separationpower with unmatched inertness to virtually any type of analyte.

Functional Group Endcapped Particle Size (µm) Pore Size (Å)Surface Area

(m2/g)Carbon Load

(%)pH Range

Octadecyl Yes 3, 5 100 350 14 1 - 10

InertSustain™ C18 phase

Figure 1.

Figure 2. Basic compounds

Inert - Superior peak shape with better resolutionSustain - Wide pH analysis with endurance at high pHC18 - Strong retentivity, low operating pressure



InertSustain™ C18 (continued)

Figure 5. Chelating compoundsFigure 4. Acidic compounds

Figure 3.

Wide pH compatibility with long column lifetime

As shown in Figure 3 above, InertSustain™ C18 maintained high efficiency and peak shape for 300 hours while other “widepH” column brands failed.

Please see pg. 155 for ordering and pricing information



Inertsil® 3 Series


The Inertsil® 3 series from GL Sciencesʼ represents a major advance in performance over the original Inertsil® 2 Series.Inertsil® 3 Series phases are based on a purer, higher surface area silica which is specially manufactured to provide maximum bond-ed phase coverage. The result is a series of columns which provide excellent peak shapes using simple eluents while operating atlow pressure.


(m2/g)Carbon Load

(%)

SIL-100Å - 3, 5 100 450 -ODS-3 Yes 2, 3, 4, 5 100 450 15ODS-3V Yes 5 100 450 15ODS-P No 5 100 450 29ODS-EP No 5 100 450 9Peptide C18 Yes 5 100 450 ProprietaryODS-Sprint Yes 3, 5 100 450 8.5Sulfa C18 Yes 3, 5 100 450 15C8-3 Yes 2, 3, 5 100 450 9Ph-3 No 2, 3, 5 100 450 9.5CN-3 No 5 100 450 14NH2 No 5 100 450 8Diol No 5 100 450 22HILIC No 5 100 450 20AX No 5 100 450 17CX No 5 100 450 14

• Excellent reproducibility• Exceptional stability and column lifetime• Low column back pressure• Ultra pure and rigid silica• Rigorous physical property tests• High inertness

High reproducibility with longer column lifeRigorous quality control of the physical properties of the basesilica, proprietary bonding and endcapping chemistry, as well asexcellent chromatographic tests for inertness and selectivity,contribute to the production of columns with good reproducibilityand long column lifetimes.The inherent lower column back pressure of Inertsil® ODS-3enables a 5µm, 250mm x 4.6mm column to be operated below10Mpa even when using water/methanol as the mobile phase.

Rigorous tests for base silicaInertsil® base silica is manufactured using the purest reagentsavailable, in a process that excludes contaminants during the for-mation of the silica bead structure. The resulting base silica isultra pure and shows improved efficiency and sample recoveryfor samples that interact with metals.The silica particles are rigid and have a smooth surface, pre-venting structural fragmentation when operated at pressures inexcess of 30Mpa.To ensure extremely low metal levels of the silica, analysis byAtomic Emission and ICP is applied to every batch. ScanningElectron Microscopy is also used for the assurance of the surfacesmoothness. Particle sizes are measured by NitrogenAdsorption and Laser Ray Particle Analyzer. Every batch of syn-thesized silica gel is subject to rigorous inspection, and onlythose batches meeting the strict specifications are used for thesubsequent bonding procedure.

Inertsil® ODS-3

Now available in 2µm for UHPLC,see page 139 for ordering information



High inertnessIt is well known that residual silanol groups on the surface of thesilica gel will cause severe peak tailing and adsorption, especial-ly with nitrogen-containing compounds. In the production ofInertsil® ODS-3, the silanols are eliminated through a proprietarybonding-endcapping technology developed at GL Sciences. Toensure maximum inertness, each batch of Inertsil® ODS-3 mustpass a new quality control program using 29Si NMR.The 29Si NMR spectrum (Fig. 1 next page) shows that isolatedsilanols on Inertsil® ODS-3 packings have been completely elim-inated, thereby ensuring excellent inertness and optimum perfor-mance of the phase. There are many new generation ODSphases on the market which still show residual silanols using thistest.Inertsil® ODS-3 shows excellent inertness for both basic andacidic compounds. In addition, chelating compounds also elutewith good peak shape owing to the high purity of the base silica.As typical examples of these compound types, pyridine, ethyl-aniline, benzylamine and aminopyridine (basic compounds),formic acid and acetic acid (acids) and oxine copper (chelatingcompound) are generally employed for QC tests. Good peakshapes are obtained on Inertsil® ODS-3 columns. Conversely,there are many columns on the market which are referred to asnew generation columns but show significant tailing for thesecompounds (see Fig. 2).

High durabilityA long column lifetime is one of the most important factors con-tributing to cost efficiency of an analysis. In general, routine usein the pH range of 2 to 7.5 is recommended for silica-based pack-ings. The durability of Inertsil® ODS-3 at pH 2 and pH9 has beentested.

The graph below shows the variations in retentivity of naphtha-lene on Inertsil® ODS-3 compared with other ODS columns.Inertsil® ODS-3 was stable at pH 2 (0.1% TFA) over 500 hours.

Figure 1. Comparison of commercially available ODS packings, using 29Si NMR spectra (referenced from previous page)

Figure 2. Comparison between ODS-3 and commercially available ODS columns

Comparison between ODS-3and commercially availableODS columns

Inertsil® ODS-3 ODS-A ODS-B ODS-C ODS-D

Column Size: 250mm x 4.6mm Eluent: 0.1% H3PO4(v/v)Flow: 1.0mL/min Temp: 40°CDetection: UV210nM Inj. Vol: 4µLSample: 1. Formic Acid 0.1% (v/v) 2. Acetic Acid 0.1% (v/v)Formatic Acid and

Acetic Acid TEST

Inertsil® ODS-3 (continued)



Inertsil®ODS-3VA validated Inertsil® columnValidated LC analysis requires validated instruments and LCcolumns. Unless your column is manufactured in a validatedprocess, especially when changing from old to new columns,your analytical results may vary significantly and a time-consum-ing preparation of validation documents has to be completed onall such occasions. Inertsil® ODS-3V columns are alreadyvalidated and include a Manufacturerʼs Validation Certificate sothat you can eliminate unnecessary work. GL Sciences hasdeveloped a program to manufacture, test and guaranteevalidated high performance LC columns that offer completereliability from column to column, batch to batch, time after time.

Each lot of packing undergoes a series of critical analytical teststo confirm:

1) the absence of surface silanols2) minimal metallic residues3) inertness to basic compounds4) reproducible separationsIn Figure 1 the separation of a series of antidepressant drugsdemonstrates the inert nature of Inertsil® ODS-3.

• Polymerically bonded C18• Improved separation of planar compounds• Useful for carotenoid anaylsis• Separation of the 16 EPA PAHs

Inertsil® ODS-P is a polymerically bonded phase which isdensely bonded with octadecylsilane groups. It shows uniqueseparation capabilities and offers an alternative selectivity toInertsil® ODS-3. Inertsil® ODS-P is particularly suited to the sep-aration of polycyclic aromatic hydrocarbons, and shows addi-tional retentivity for planar compounds.

Figure 2 shows the separation of components from toad venom.Figure 3 shows the separation of terphenyl isomers on the threetypes of ODS phases. On the polymeric Inertsil® ODS-P phase,the planar p-terphenyl is retained longer than the non-planaro- and m- isomers.

Inertsil® ODS-P

Figure 2. Separation of toad venom

minutes

1. Bufalin

2. Cinobufagin

Column: Inertsil® ODS-P (250mm x 4.6mm)Eluent: CH3CN - 0.1% H3PO4 (45:55)Flow rate: 1ml/minTemperature: 40°C

1

2

3

4

Column dimensions: 250mm x 4.6 (mm) Flow rate : 1ml/min.Eluent : CH3OH - 25mM KH2PO4, pH 6 (60:40) Temperature : 25°C

Figure 1. Separation of antidepressant drugs onInertsil® ODS-3V

1 2

3

1. Norephedrine2. Nortriptyline3. Toluene4. Imipramine5. Amitriptyline

54

Column: Inertsil® ODS-P (150mm x 4.6mm)Eluent: CH3CN - H2O (85:15)Flow rate: 1ml/minTemperature: 40°C

Figure 3. Separation of terphenyl isomers

minutes minutes minutes

o-m

-

m- m

-p-p-p-

o- o-

o-terphenyl(non-planar)

Monomeric(Intersil ODS-3)

Intermediate(Intersil ODS-2)

Polymeric(Intersil ODS-P)

m-terphenyl(non-planar)

p-terphenyl(planar)



• C18 with polar embedded group• Compatible with 100% aqueous eluents• Alternative selectivity to C18• Ideal for LC-MS

Inertsil® ODS-EP contains a polar functional group embeddedbetween the silica surface and the C18 group. The embeddedpolar group makes the C18 phase stable in 100% aqueous elu-ents without “phase collapse”. This phase is also extremely basedeactivated and provides superior peak shape for acids andbases in organic eluents as well as acidified eluents typicallyused in LC-MS.

Inertsil® ODS-EP

Figure 1. Naphthalene sample for retention and peak shape comparison

Column : Inertsil® ODS-EP, 5µm, 150mm × 4.6mmMobile Phase: Acetonitrile:Water = 64:35Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV254 nm

Peak identification1. Acetophenone2. Benzene3. Toluene4. Naphthalene

Figure 2. Selectivity test sample comparison

Column : Inertsil® ODS-EP, 5µm, 150mm × 4.6mmMobile Phase: Acetonitrile:Water = 70:30Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV265 nm

Peak identification1. Ethylbenzene2. Naphthalene3. n-Propylbenzene4. n-Butylbenzene5. Anthracene

Figure 3. Tanaka test sample(Comparison of hydrogen bonding capacity,hydrophobicity and selectivity)

Column : Inertsil® ODS-EP, 5µm, 150mm × 4.6mmMobile Phase: Methanol:Water = 80:20Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV254 nm

Peak identification1. Uracil2. Caffeine3. Phenol4. n-butylbenzene5. n-Amylbenzene6. o-Terphenyl7. Triphenylene



Inertsil® HILICHydrophilic Interaction Chromatography is a variation of normal-phase mode where a high concentration of non-polar solvent isused in a mobile phase. It is an ideal method for separating andretaining highly polar analytes unretained by traditional reversed-phase mode. HILIC columns become widely used for criticalseparations in demanding drug metabolism, drug discovery andcombinatorial chemistry. In addition, volatile mobile phases areused for HILIC mode and afford enhanced ESI-MS sensitivityresulting in lower limits of detection.

Application for HILIC mode- Amino acids- Water-soluble vitamins- Drug metabolites and drug discovery- Combinatorial chemistry- Promoted retention of compounds unretained by reversed-

phase mode

Advantages of HILIC mode- Low column back pressure analyses can be performed due to

high organic solvent composition- A high organic solvent concentration of the mobile phase will

lead to a high sensitivity LC-MS analysis- The benefit of less ion suppression effect in LC-MS analysis- Precision determination for polar compounds due to the fast

elution of hydrophobic contents

Column : Inertsil® HILIC 5µm, 250mm × 3.0mmEluent : Acetonitrile (0.1% TFA)/H2O (0.1% TFA) = (90/10), (80/20), (60/40), 20/80)Flow Rate : 0.4 mL/minCol. Temp. : 40°CDetection: UV 254 nMSample Size: 20µL

Sample:1. Thiamine (8ppm)2. Nicotinamide (8ppm)3. L-Ascorbic acid (80ppm)4. Riboflavin (8ppm)

Figure 2. Water soluble vitamins

Column : Inertsil® HILIC 5µm, 250mm × 3.0mmEluent : Acetonitrile 10mM KH2PO4 (10%ACN) = (85/15),(80/20),(70/30),(50/50),(30/70)Flow Rate : 0.4 mL/minCol. Temp. : 40°CDetection: UV 210 nMSample Size: 1mL

Sample:1. Phenylalanine (100ug/mL)2. Methionine (500ug/mL)3. Valine (4mg/mL)4. Alanine (4mg/mL)5. Serine (8mg/mL)

Figure 3. Amino acids

Column : Inertsil® HILIC, 5µm, 150mm × 4.6mmMobile Phase : Acetonitrile/H2O (85:15, w/w)Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: RI

Peaks:

1. Maltose2. Maltotriose3. Maltotetraose4. Maltopentaose5. Maltohexaose6. Maltoheptaose

Figure 1. Separation of oligosaccharide



Inertsil® ODS-Sprint• Well balanced retention of both polar and

non polar compounds• Superior inertness and base deactivation• Compatible with completely aqueous or neat

organic eluents• Low operating back pressure• 20mm x 2.1mm column with new Sprint HG

hardware is ideal for rapid LC-MS separations

Inertsil® ODS-Sprint is an important new addition to the Inertsil 3column series. Inertsil ODS-Sprint is extremely base deactivatedand optimally bonded to retain polar compounds without exces-sive retention of non-polar compounds. This allows you toachieve improved separations faster.ODS-Sprint operates at low back pressure even at high flowrates, shows outstanding longevity and is reproducible fromcolumn to column and batch to batch.To complement this new phase, GL Sciencesʼ has created azero-dead-volume column hardware format, known as Sprint-HGhardware, further enhancing peak symmetry.For additional information, please contact Canadian LifeScience.

Moderate retentivityGL Sciencesʼ newly developed chemical bonding technologyallows Inertsil® ODS-Sprint to retain hydrophilic compounds, butelutes hydrophobic compounds faster. It offers 1/2 of Inerstil®ODS-3 retention for hydrophobic compounds (see Figure 1).

Short equilibration timeA highly uniformed low carbon loading ODS enables shorterequilibration time for gradient analysis and can be used for high-throughputs. The time for purging and cleaning the column canbe reduced in isocratic analysis.In Figure 2, Inertsil® ODS-SP shows a complete equilabrationafter 10 mins with stable retentivity.

Figure 1. Elution of hydrophobic compoundsColumn : Inertsil® ODS-SP, 5µm, 250mm × 4.6mmMobile Phase: CH3OH/H2O = 80/20, v/vFlow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV254 nM)

Figure 2. Equilibration time comparisonColumn : Inertsil® ODS-SP, 5µm, 250mm × 4.6mmMobile Phase: CH3CN/H2O = 5/95 (5min hold) - (7min) - 100/0 (5min hold),v/vFlow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV215 nM Sample:

1. Methamidophos2. Acephate



Inertsil® ODS-SprintSharp peak shapeSharp peak shapes can be observed due to GL Sciencesʼ newly-developed bonding technology and column hardware.3µm packed columns are also available for further sharp peakshapes.

Inertsil® ODS-sprint does not show any tailing with complete baseline separation, resulting in excellent quantitative determination.Peak tailing of large volume compounds (unreacted derivatizingreagent) disturbed the detection of the target compound onCompetitor E, F and G columns (see Figure 3).

No adsorption of peaksGL Sciencesʼ intensive endcapping is performed to eliminateresidual silanols, creating an exceptionally inert column forreverse-phase chromatography. Excellent peak shapes for awide range of polar analytes, including both basic and acidiccompounds, can be obtained.Inertsil® ODS-Sprint shows excellent peak shapes of basic andacidic compounds. Other competitorʼs columns are showing tail-ing peaks of both compounds. Some columns may be able toanalyze basic compounds, but show peak tailing for acidic com-pounds (see Figure 4).

Figure 3. Analysis of derivatized products

Column : Inertsil® ODS-SP, 5µm, 150mm × 4.6mmMobile Phase: CH3CN/H2O = 55/45, v/vFlow Rate : 1.0 mL/min SampleCol. Temp. : 40°C 1. DNPH-FormadehydeDetection: UV360 nM) 2. DNPH-Acetoaldehyde

Figure 4. Analysis of basic compounds

Column : Inertsil® ODS-SP, 5µm, 250mm × 4.6mmMobile Phase: CH3OH/20mM Phosphate buffer (pH 7.6) = 10/90, v/vFlow Rate : 1.0 mL/minCol. Temp. : 40°C SampleDetection: UV254 nM 1. 4-aminopyridine

2. 3-aminopyridene3. 2-aminopyridine

Stable performanceTo retain water-soluble organic compounds, the use of mobilephases that contain little or no organic modifier are sometimesneeded. Under these highly aqueous conditions, Inertsil®ODS-Sprint shows excellent reproducible retention behaviourand high resistance to buffer or acid additives from lot-to-lot (seeFigure 5).

Testing procedure1) 1st analysis conducted after introducing eluent for 20 minutes2) Stop flow for 15 minutes3) 2nd analysis conducted after introducing eluent for 10 minutes4) Stop flow for 15 minutes

Figure 5. Stop-flow test using 100% water eluent

Column : Inertsil® ODS-SP, 5µm, 250mm × 4.6mm SampleMobile Phase: H3O 1005 1.CytosineFlow Rate : 1.0 mL/min 2. UracilCol. Temp. : 40°C 3. GuanineDetection: UV254 nM) 4. Thymine

5. Adenine



Sulfa C18Analysis of antibiotic drugs and drug residues in products of ani-mal origin has become an important element of ensuring foodsafely.Inertsil® Sulfa C18 is suitable not only for the analysis of sulfadrugs but also for the screening of synthetic antibacterial agentswhich are directed in the food safety investigative guidelines.

Each batch of Inertsil® Sulfa C18 is tested for the effective sepa-ration of sulfa drugs, as shown in Figure 1 below, and is provid-ed with a copy of the chromatogram.

System: GL-7400 LC SYSTEMColumn : Inertsil® SULFA C18, 3µm, 150mm × 4.6mmEluent : A) CH3CN/ CH3COOH = 777.66/3.15, w/w

B) CH3COOH/H2O = 3.15/997, w/wA/B - 0/100 -33min- 33/67 -3min- 90/10 -4min- 90/10

Flow Rate: 1.0mL/minCol. Temp. : 40°CDetection: UV 270 nMInjection Vol: 10µLSample:1. Sulfanilamide 8. Sulfamethoxypyridazine2. Sulfacetamide 9. Sulfamonomethoxine3. Sulfadiazine 10. Sulfachlorpyridazine4. Sulfathiazole 11. Sulfamethoxazole5. Sulfapyridine 12. Sulfabenzamide6. Sulfameradine 13. Sulfadimethoxine7. Sulfacymidine 14. Sulfaquinoxalin

15. Sulfanitran

Figure 1. Analysis of 15 sulfa reagents

Figure 2. Inertness comparison

Figure 3. Analysis of antibacteria agents directed in the food sanitation investigation guideline

Antibacterial agents, including sulfadrugs, consist of polar functionalgroups which can cause adsorption onthe packing material.High inertness to basic and acidiccompounds enables Inertsil® Sufa C18to effectively analyze antibacterialagents.

Column :Inertsil® Sulfa, 5µm, 250mm × 4.6mmMobile Phase: 0.1% H3PO4 v/vFlow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV210 nmSample Size: 4 µLSample1) Formic Acid 0.1% (v/v)2) Acetic Acid 0.1% (v/v)

Column :Inertsil® Sulfa, 5µm, 250mm × 4.6mm

Mobile Phase: 0.1% CH3OH/ H2O 30/70Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV254 nmSample Size: 4 µLSample1) Pyridine 0.09 mg/mL2) Phenol 0.41 mg/mL



• Unique selectivity in both reversed-phase andnormal-phase

• Applications for amino acids, water-solublevitamins and sugars

The Inertsil® Diol phase is a dihydroxypropyl bonded silica whichcan be used in both reversed-phase and normal-phase modes.The retentivity is higher than that of silica in normal-phase mode.Figure 1 shows the analysis of free amino acids on an Inertsil®Diol column.

The new Inertsil® Peptide C18 column was developed for theanalysis of Peptide mapping. 100Å pore size high purity silicagels are bonded with octadecyl groups and the residual silanolsare drastically reduced. Metabolic analysis to Peptide analysisare performed with great reproducibility on Inertsil® Peptide C18.Figure 2 shows the application of this column for the analysis ofcytochrome C tryptic digest.

Inertsil® Diol Inertsil® Peptide C18

• High theoretical plate number• Silica base anion exchange columns• High reproduciblity• Exceptional stability and long column lifetime

Figure 1. Analysis of free amino acids

Figure 2. Analysis of cytochrome C on Inertsil® Peptide C18

AX bonded phase structure

CX bonded phase structure

Figure 3. Adenosine and adenosine mono phosphate

Improved stability versusconventional SAX and SCX

columns such asPartisil SAX, SCX

Column: Inertsil® Diol (250mm x 4.6mm)Eluent: CH3CN - H2O (80:20)Flow rate: 1ml/minTemperature: 40°C

1. Phenylalanine2. Methionine3. Valine4. Dopa5. Alanine6. Serine7. Citrulline

Inertsil® AX (Anion Exchange), CX (Cation Exchange)



• Virtually free of trace metals (99.999% pure silica gel)• Rigorous quality control tested for inertness• Excellent for basic drugs and amines• High reproducibility from batch to batch, column to column• Unrivaled for chemical and physical stability


Inertsil® 2 Series

Phase Functional Group Endcapped Particle Size (µm) Pore Size (Å)Surface Area

(m2/g)Carbon Load

(%)

ODS-2 Yes 5 150 320 18.5C8 Octyl Yes 5 150 320 10.5C4 Butyl Yes 5 150 320 7.5Phenyl Phenyl Yes 5 150 320 10SIL-150A Bare silica No 5 150 320 -

The original Inertsil® 2 series phases created the high purity sili-ca revolution in the the late 1980ʼs. These phases utilize a99.999% pure silica gel which is extremely durable and free fromdents and cracks which can cause premature column failure.The original Inertsil® 2 series of columns remain some of themost useful and popular on the market today.

Figure 2. Oxine copper

Column : Inertsil® C4, 5µm, 150mm × 4.6mmMobile Phase:CH3CN/20mM IPPC-12A+20mM NaH2PO4(pH3.5 by H3PO4) = 30/70IPCC-12A: Sodium 1-Dodecylsulfate

Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV240 nM

Solutes1. Asulam2. Simazine3. Thiuram4. Oxine-copper

Figure 3. Analysis of basic compounds

Column :Inertsil® Ph, 5µm, 150mm × 4.6mmMobile Phase:A) CH3CN/0.1% H3PO4 = 25/75B) CH3CN/0.1% H3PO4 = 75/25A/B 100/0 (25min) 0/100Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV210 nM

Solutes1. Propionic acid2. Butyric acid3. Valeric acid4. Caproic acid5. Heptylic acid6. Caprylic acid7. Capric acid8. Lauric acid9. Myristic acid10. Palmitic acid

Figure 1. Dicarboxylic acids

Column : Inertsil® ODS-2, 5µm, 150mm × 4.6mmMobile Phase: A) CH3CN/0.1% H3PO4 = 2/98

B) CH3CN/0.1% H3PO4 = 45/55A/B 100/0 (25min) 0/100

Flow Rate : 1.0 mL/minCol. Temp. : 40°CDetection: UV210 nM

Solutes1. Malonic acid2. Succinic acid3. Glutaric acid4. Adipic acid5. Pimelic acid6. Azelaic acid7. Sebacic acid



GL Sciences catalogue andINERTSearch™ CD-ROM,

containing 385 Inertsil® applications,available on request

Capillary and Nano Columns• Highly inert PEEK armoured fused silica tubing• Trace detection with high sensitivity• Analysis of expensive samples• Applications: LC-MS, Proteins, Peptides,

Proteomics• Capillary EX available in .3mm, .5mm

and .7mm ID’s• Capillary EX-nano available in .05mm, .075mm,

.1mm and .2mm ID’s

These products continue to expand the separation power of theunified Inertsil® column series all based on the same outstandinghigh purity silica that is the backbone of Inertsil®ʼs success.

Inertsil® Capillary and Nano columns are available inInertsil® 4 Series, InertSustain, Inertsil® 3 series, includingPeptides C18 (p.150) for Peptide mapping, and Inertsil® WidePore series (p.153).

Inertsil® PREP ColumnsInertsil® PREP packing materials are dedicated to preparativeapplications. The semi-prep columns (6mm ID) have identicalseparation characteristics to preparative columns (50mm and100mm ID) due to the high performance packing process usedby GL Sciences. This permits a smooth change from a 6mm IDcolumn, used to establish operating parameters, to a largerpreparative column if the same linear velocities are employed.

See page 161 for all ordering information

Phase Particle Size (µm) Endcapped PH RANGE Pore Size (Å)Surface Area

(m2/g)Carbon Load

(%)

PREP-ODS 10 Yes 2 - 7.5 100 350 20PREP-C8 10 Yes 2 - 7.5 100 350 10PREP-SIL 10 - 2 - 7.5 100 350 -ECONO PREP-ODS 40 Yes 2 - 7.5 80 400 17ECONO PREP-SIL 40 - 2 - 7.5 80 400 -

Preparative sizes also available in Wide Pore Phases. Please enquire.

New GL Sciences cataloguecoming soon!



Inertsil® Wide Pore Phases• High purity 300Å silica phases• Excellent reproducibility• Good physical and chemical stability• Available in preparative sizes

The Inertsil® WP300 series of columns has been developed forthe analysis and purification of proteins and peptides.

High durability under TFA eluent conditionsInertsil® WP300 Series shows high durability against acidic elu-ents, including TFA solutions (see Fig. 1).Protein and peptide analyses can beperformed using formic acid as a modifierExceptional inertness of Inertsil® WP300 Series prevents irre-versible adsorption of proteins under formic acid eluent condi-tions, providing optimum conditions for LC-MS.

Column choice depends on target ofmolecular weightInertsil® WP300 C8 and C18 show high separation efficiency forpeptides and proteins of medium molecular size.Inertsil® WP300 C4, having high partition efficiency for veryhydrophobic proteins, elutes them with sharp peak shapes.

Inertsil®WP300 C4Recovery of proteins with highhydrophobicityOn Inertsil® WP300 C4 you can get a linear recovery under diffi-cult eluent conditions. Three typical proteins with high hydropho-bicity show good linear recovery (see Fig. 1).Inertsil®WP300 DiolInertsil® WP300 Diol is designed for GFC collection and purifica-tion of proteins (see Fig. 2).

Inertsil® wide pore phases

Figure 1. Linear recovery of 3 typical proteins

Column: Inertsil® WP300 C4, 150mm x 4.6mm, 5µmEluent: A: 0.2% Formic Acid

B: CH3CN / H20 (90:10)(0.2% Formic acid in Solvent B)A/B = 80/20 20min. 20/80

Flow Rate: 1.0mL/minCol. Temp: 30°CDetector: UV 280nM


(m2/g)Carbon Load

(%)pH Range

SIL - 5 300 150 - 2 - 7.5C4 Yes 5 300 150 3 2 - 7.5C8 Yes 5 300 150 8 2 - 7.5C18 Yes 5 300 150 9 2 - 7.5Diol No 5 300 150 9 2 - 7.5

Figure 2. Calibration curve of protein byInertsil® WP300 Diol

Column: Inertsil® WP300 Diol, 250mm x 4.6mm, 5µmMobile Phase: 200mM Sodium Phosphate (pH=6.9)Flow Rate: 0.5mL/minTemp: 30°CDetector: UV 280nM1. Thyroglobulin 2. BSA 3. Lysozyme 4. Insulin chainB



Ordering Information - Inertsil® 4 SeriesMicrobore (2.1mm ID) columns (1mm ID also available - please contact us)

1 Other dimensions available. Guard columns also available.2 Use with holder IN5020-08500 ($186)3 20mm length cartridges available for 3mm and 4mm ID’s. Use with holder IN5020-08550 ($186)4 Conventional Guard Columns available for 2µm columns. Please enquire.5 Holder/Cartridge Sets available (1 holder, 2 cartridges) for 10mm and 20mm length cartridges (3mm and 4mm ID’s only)

Inertsil® PhaseColumn Dimensions1 (mm)

30 x 2.1 50 x 2.1 75 x 2.1 100 x 2.1 150 x 2.1 250 x 2.12µm $686 $699 $773 $847 $968 - -C8-4 IN5020-81280 IN5020-81282 IN5020-81283 IN5020-81284 IN5020-81285 - -

ODS-4 IN5020-81200 IN5020-81202 IN5020-81203 IN5020-81204 IN5020-81205 - -

30 x 2.1 50 x 2.1 75 x 2.1 100 x 2.1 150 x 2.1 250 x 2.1Guard Cartridges2 (2/pk)

(10 x 2.1mm)3

3µm $460 $504 $560 $605 $650 $717 $3215

C8-4 IN5020-03971 IN5020-03972 IN5020-03973 IN5020-03974 IN5020-03975 IN5020-03976 IN5020-08518-3µ-C8-4

ODS-4 IN5020-04011 IN5020-04012 IN5020-04013 IN5020-04014 IN5020-04015 IN5020-04016 IN5020-08518-3µ-ODS4

5µm $426 $471 $516 $560 $605 $650 $3215



Medium bore (3.0mm ID) columns

Analytical (4.6mm ID) columns

Inertsil® PhaseColumn Dimensions1 (mm) Guard Cartridges2 (2/pk)

(10 x 4.0mm)330 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 250 x 4.63µm $460 $504 $560 $605 $650 $717 $2965


5µm $426 $471 $516 $560 $605 $650 $2965




30 x 3.0 50 x 3.0 75 x 3.0 100 x 3.0 150 x 3.0 250 x 3.02µm $686 $699 $773 $847 $968 - -C8-4 IN5020-81290 IN5020-81292 IN5020-81293 IN5020-81294 IN5020-81295 - -

ODS-4 IN5020-81210 IN5020-81212 IN5020-81213 IN5020-81214 IN5020-81215 - -

30 x 3.0 50 x 3.0 75 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0Guard Cartridges2 (2/pk)

(10 x 3.0mm)3

3µm $460 $504 $560 $605 $650 $717 $2965



5µm $426 $471 $516 $560 $605 $650 $2965

C8-4 IN5020-04061 IN5020-04062 IN5020-04063 IN5020-04064 IN5020-04065 IN5020-04066 IN5020-08515-5µ-C84


Semi-Preparative and Preparative (6mm - 50mm ID) columns also available.Please contact Canadian Life Science for further details on 6mm - 50mm ID Inertsil® columns.

(see page 161 for more preparative columns)


ORDERING INFORMATION - INERTSIL® (GL Sciences)

Ordering Information - InertSustain™

Microbore (2.1mm ID) columns (1mm also available - please contact us)


(10 x 2.1mm)330 x 2.1 50 x 2.1 75 x 2.1 100 x 2.1 150 x 2.1 250 x 2.13µm $460 $504 $560 $605 $650 $717 $321InertSustain IN5020-07411 IN5020-07412 IN5020-07413 IN5020-07414 IN5020-07415 IN5020-07416 IN5020-19350

5µm $426 $471 $516 $560 $605 $650 $321InertSustain IN5020-07311 IN5020-07312 IN5020-07313 IN5020-07314 IN5020-07315 IN5020-07316 IN5020-19349

Medium bore (3.0mm ID) columns


(10 x 2.1mm)330 x 3.0 50 x 3.0 75 x 3.0 100 x 3.0 150 x 3.0 250 x 3.03µm $460 $504 $560 $605 $650 $717 $2965

InertSustain IN5020-07421 IN5020-07422 IN5020-07423 IN5020-07424 IN5020-07425 IN5020-07426 IN5020-19150

5µm $426 $471 $516 $560 $605 $650 $2965





(10 x 4.0mm)330 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 250 x 4.63µm $460 $504 $560 $605 $650 $717 $2965


5µm $426 $471 $516 $560 $605 $650 $2965






Ordering Information - Inertsil 3 SeriesMicrobore (2.1mm ID) columns (1mm ID also available - please contact us)


(10 x 1.5mm)350 x 2.1 100 x 2.1 150 x 2.1 250 x 2.13µm $507 $567 $605 $641 $3215

C8-3 IN5020-04812 IN5020-04814 IN5020-04815 IN5020-04816 IN5020-08518-3µ-C8-3

ODS-3 IN5020-04412 IN5020-04414 IN5020-04415 IN5020-04416 IN5020-08518-3µ-ODS3

ODS-P IN5020-04662 IN5020-04664 IN5020-04665 IN5020-04666 IN5020-08518-3µ-ODSP

ODS-Sprint IN5020-02812 IN5020-02814 IN5020-02815 IN5020-02816 IN5020-08518-3µ-ODS-SP

Ph-3 IN5020-05012 IN5020-05014 IN5020-05015 IN5020-05016 IN5020-08518-3µ-Ph3

CN-3 IN5020-05262 IN5020-05264 IN5020-05265 IN5020-05266 IN5020-08518-3µ-CN3

NH2 IN5020-05462 IN5020-05464 IN5020-05465 IN5020-05466 IN5020-08518-3µ-NH2

Diol IN5020-05412 IN5020-05414 IN5020-05415 IN5020-05416 IN5020-08518-3µ-DIOL

SIL-100Å IN5020-04212 IN5020-04214 IN5020-04215 IN5020-04216 IN5020-08518-3µ-S10

HILIC IN5020-07762 IN5020-07764 IN5020-07765 IN5020-07766 IN5020-08518-3µ-HILIC

- $616 $641 - $3215

Sulfa C18 - IN5020-07504 IN5020-07505 - IN5020-08518-3µ-SULFA

4µm $500 $556 $592 $630 $3215


5µm $448 $493 $527 $560 $3215

C8-3 IN5020-04912 IN5020-04914 IN5020-04915 IN5020-04916 IN5020-08518-5µ-C8-3



ODS-EP IN5020-02612 IN5020-02614 IN5020-02615 IN5020-02616 IN5020-08518-5µ-ODSEP








$518 $616 $690 $740 $3215

AX IN5020-07212 IN5020-07214 IN5020-07215 IN5020-07216 IN5020-08518-5µ-AX

$567 $666 $764 $838 $3215

CX IN5020-07112 IN5020-07114 IN5020-07115 IN5020-07116 IN5020-08518-5µ-CX

- - $581 $605 $3215

Sulfa C18 - - IN5020-07545 IN5020-07546 IN5020-08518-5µ-SULFA

$493 $543 $581 $616 $3215

Peptide C18 IN5020-08022 IN5020-08024 IN5020-08025 IN5020-08026 IN5020-08518-PEPTIDES




30 x 2.1 50 x 2.1 75 x 2.1 100 x 2.1 150 x 2.1

2µm $646 $659 $733 $807 $928

C8-3 IN5020-84930 IN5020-84931 IN5020-84932 IN5020-84933 IN5020-84934

ODS-3 IN5020-84650 IN5020-84652 IN5020-84653 IN5020-84654 IN5020-84655

Ph-3 IN5020-85130 IN5020-85131 IN5020-85132 IN5020-85133 IN5020-85134

1 Other dimensions available. Guard columns also available. 2 Use with holder IN5020-08500 ($186)3 20mm length cartridges available for 3mm and 4mm ID’s. Use with holder IN5020-08550 ($186) 4 Conventional Guard Columns available for 2µm columns. Please enquire.5 Holder/Cartridge Sets available (1 holder, 2 cartridges) for 10mm and 20mm length cartridges (3mm and 4mm ID’s only)



Ordering Information - Inertsil® 3 Series (continued)Medium bore (3.0mm ID) columns


(10 x 3.0mm)350 x 3.0 100 x 3.0 150 x 3.0 250 x 3.03µm $507 $567 $605 $641 $2965

C8-3 IN5020-04822 IN5020-04824 IN5020-04825 IN5020-04826 IN5020-08515-3µ-C8-3ODS-3 IN5020-04422 IN5020-04424 IN5020-04425 IN5020-04426 IN5020-08515-3µ-ODS3ODS-P IN5020-04672 IN5020-04674 IN5020-04675 IN5020-04676 IN5020-08515-3µ-ODSPODS-Sprint IN5020-02822 IN5020-02824 IN5020-02825 IN5020-02826 IN5020-08515-3µ-ODS-SPPh-3 IN5020-05022 IN5020-05024 IN5020-05025 IN5020-05026 IN5020-08515-3µ-Ph3CN-3 IN5020-05272 IN5020-05274 IN5020-05275 IN5020-05276 IN5020-08515-3µ-CN3NH2 IN5020-05472 IN5020-05474 IN5020-05475 IN5020-05476 IN5020-08515-3µ-NH2Diol IN5020-05422 IN5020-05424 IN5020-05425 IN5020-05426 IN5020-08515-3µ-DIOLSIL-100Å IN5020-04222 IN5020-04224 IN5020-04225 IN5020-04226 IN5020-08515-3µ-S10HILIC IN5020-07772 IN5020-07774 IN5020-07775 IN5020-07776 IN5020-08515-3µ-HILIC

- $616 $641 - $2965

Sulfa C18 - IN5020-07514 IN5020-07515 - IN5020-08515-3µ-SULFA4µm $500 $556 $592 $630 $2965

ODS-3 IN5020-04622 IN5020-04624 IN5020-04625 IN5020-04626 IN5020-08515-4µ-ODS35µm $448 $493 $527 $560 $2965

C8-3 IN5020-04922 IN5020-04924 IN5020-04925 IN5020-04926 IN5020-08515-5µ-C8-3

ODS-3 IN5020-04522 IN5020-04524 IN5020-01751 IN5020-01752 IN5020-08515-5µ-ODS3ODS-P IN5020-04722 IN5020-04724 IN5020-04725 IN5020-04726 IN5020-08515-5µ-ODSPODS-EP IN5020-02622 IN5020-02624 IN5020-02625 IN5020-02626 IN5020-08515-5µ-ODSEPODS-Sprint IN5020-02722 IN5020-02724 IN5020-02725 IN5020-02726 IN5020-08515-5µ-ODS-SPPh-3 IN5020-05122 IN5020-05124 IN5020-05125 IN5020-05126 IN5020-08515-5µ-Ph3CN-3 IN5020-05322 IN5020-05324 IN5020-05325 IN5020-05326 IN5020-08515-5µ-CN3NH2 IN5020-05522 IN5020-05524 IN5020-05525 IN5020-05526 IN5020-08515-5µ-NH2Diol IN5020-05622 IN5020-05624 IN5020-05625 IN5020-05626 IN5020-08515-5µ-DIOLSIL-100Å IN5020-04322 IN5020-04324 IN5020-04325 IN5020-04326 IN5020-08515-5µ-S10HILIC IN5020-07712 IN5020-07714 IN5020-07715 IN5020-07716 IN5020-08515-5µ-HILIC

- - $590 $628 $2965

ODS-3V IN5020-01821 IN5020-01822 IN5020-08515-5µ-ODS3$518 $616 $690 $740 $2965

AX IN5020-07222 IN5020-07224 IN5020-07225 IN5020-07226 IN5020-08515-5µ-AX$567 $666 $764 $838 $2965

CX IN5020-07122 IN5020-07124 IN5020-07125 IN5020-07126 IN5020-08515-5µ-CX- - $581 $605 $2965

Sulfa C18 - - IN5020-07555 IN5020-07556 IN5020-08515-5µ-SULFA$493 $543 $581 $616 $2965



30 x 3.0 50 x 3.0 75 x 3.0 100 x 3.0 150 x 3.0

2µm $646 $659 $733 $807 $928

C8-3 IN5020-84935 IN5020-84936 IN5020-84937 IN5020-84938 IN5020-84939

ODS-3 IN5020-84660 IN5020-84662 IN5020-84663 IN5020-84664 IN5020-84665

Ph-3 IN5020-85135 IN5020-85136 IN5020-85137 IN5020-85138 IN5020-85139




Inertsil PhaseColumn Dimensions1 (mm) Guard Cartridges2 (2/pk)

(10 x 4.0mm)350 x 4.6 100 x 4.6 150 x 4.6 250 x 4.63µm $507 $567 $605 $641 $2964

C8-3 IN5020-04842 IN5020-04844 IN5020-01911 IN5020-01912 IN5020-08510-3µ-C8-3










- $616 $641 - $2964

Sulfa C18 - IN5020-07534 IN5020-07535 - IN5020-08510-3µ-SULFA

4µm $500 $556 $592 $630 $2964


5µm $448 $493 $527 $560 $2964

C8-3 IN5020-04942 IN5020-04944 IN5020-01900 IN5020-01901 IN5020-08510-5µ-C8-3



ODS-EP IN5020-02642 IN5020-02644 IN5020-02645 IN5020-02646 IN5020-08510-5µ-ODSEP








- - $590 $628 $2965

ODS-3V - - IN5020-01801 IN5020-01802 IN5020-08510-5µ-ODS3

$518 $616 $690 $740 $2964

AX IN5020-07242 IN5020-07244 IN5020-07245 IN5020-07246 IN5020-08510-5µ-AX

$567 $666 $764 $838 $2964

CX IN5020-07142 IN5020-07144 IN5020-07145 IN5020-07146 IN5020-08510-5µ-CX

- - $581 $605 $2964

Sulfa C18 - - IN5020-07575 IN5020-07576 IN5020-08510-5µ-SULFA

$493 $543 $581 $616 $2964


Ordering Information - Inertsil® 3 Series (continued)Analytical (4.6mm ID) columns

1 Other dimensions available. Guard columns also available.2 Use with holder IN5020-08500 ($186)3 20mm length cartridges available for 3mm and 4mm ID’s. Use with holder IN5020-08550 ($186)4 Holder/Cartridge Sets available (1 holder, 2 cartridges) for 10mm and 20mm length cartridges (3mm and 4mm ID’s only)






Inertsil® Phase Column Dimensions1 (mm) Guard Cartridges2 (2/pk)(10 x 1.5mm)3100 x 2.1 150 x 2.1 250 x 2.1

5µm $612 $686 $740 $321WP300-C18 IN5020-05814 IN5020-05815 IN5020-05816 IN5020-08518-5WP300C18WP300-C8 IN5020-05714 IN5020-05715 IN5020-05716 IN5020-08518-5WP300C8WP300-C4 IN5020-05864 IN5020-05865 IN5020-05866 IN5020-08518-5WP300C4WP300-Diol IN5020-05914 IN5020-05915 IN5020-05916 IN5020-08518-5WP300DiolWP300-SIL IN5020-06014 IN5020-06015 IN5020-06016 IN5020-08518-5WP300SIL

Ordering Information - Inertsil® Wide Pore Phases

Microbore (2.1mm ID) columns


5µm $612 $686 $740 $2964

WP300-C18 IN5020-05844 IN5020-05845 IN5020-05846 IN5020-08510-5WP300C18WP300-C8 IN5020-05744 IN5020-05745 IN5020-05746 IN5020-08510-5WP300C8WP300-C4 IN5020-05894 IN5020-05895 IN5020-05896 IN5020-08510-5WP300C4WP300-Diol IN5020-05944 IN5020-05945 IN5020-05946 IN5020-08510-5WP300DiolWP300-SIL IN5020-06044 IN5020-06045 IN5020-06046 IN5020-08510-5WP300SIL


1 Other dimensions available. Guard columns also available. 2 Use with holder IN5020-08500 ($186)3 20mm length cartridges available for 3mm and 4mm ID’s. Use with holder IN5020-08550 ($186)4 Holder/Cartridge Sets available (1 holder, 2 cartridges) for 10mm and 20mm length cartridges (3mm and 4mm ID’s only)5 PEEK Columns available for 150mm and 250mm lengths (for 4.6mm ID and 7.6mm ID only)



5µm $630 $630 $679 $2964

ODS-25 IN5020-ODS2-46-100 IN5020-01101-46 IN5020-01102-46 IN5020-08510-ODS2$690 $740 $2964

SIL-150A - IN5020-01011-46 IN5020-01012-46 IN5020-08510-S15C8 - IN5020-01201-46 IN5020-01202-46 IN5020-08510-C8Phenyl - IN5020-01301-46 IN5020-01302-46 IN5020-08510-Ph

$789 $838 $2964

C4 IN5020-01401-46 IN5020-01402-46 IN5020-08510-C4

Ordering Information - Inertsil® 2 Series


5µm $630 $630 $679 $3214

ODS-2 IN5020-ODS2-21-100 IN5020-01101-21 IN5020-01102-21 IN5020-08518-ODS2$690 $740 $3214

SIL-150A - IN5020-01011-21 IN5020-01012-21 IN5020-08518-S15C8 - IN5020-01201-21 IN5020-01202-21 IN5020-08518-C8Phenyl - IN5020-01301-21 IN5020-01302-21 IN5020-08518-Ph

$789 $838 $3214

C4 IN5020-01401-21 IN5020-01402-21 IN5020-08518-C4

Microbore (2.1mm ID) columns



Ordering Information - Capillary and Nano ColumnsWhen ordering, please replace ʻXʼ with the appropriate material code.

Inertsil®

3µm Phase3µm ODS-3 3µm C8-3 3µm Ph-3

3µm SIL-100A

X 3ODS3 3C8-3 3Ph3 3SIL100A

Inertsil® 3 Series

Inertsil®

5µm Phase5µm ODS-3 5µm C8-3 5µm Ph-3

5µm SIL-100A

5µm ODS-EP 5µm ODS-P 5µm CN-3 5µm NH2 5µm Diol

X 5ODS3 5C8-3 5Ph3 5SIL100A 5ODSEP 5ODSP 5CN3 5NH2 5Diol

Inertsil® Phase 5µm WP300 C18 5µm WP300 C8 5µm WP300 C4 5µm WP300 Diol 5µm WP300 SIL

X WP300 C18 WP300 C8 WP300 C4 WP300 Diol WP300 SIL

Inertsil WP300 Series

Inertsil®

PhasePeptides C18

X Peptides C18

For Peptide Mapping

Column TypeColumn Diameter

(mm)

Column Dimensions (mm) Guard Columns(2 x 0.3mm ID)50 150 250

$932 $991 $1049 -

Capillary EX-Nano1 .05 IN5020-01960-X IN5020-01961-X IN5020-01962-X -




$932 $991 - $263Capillary EX2 .3 IN5020-01870-X IN5020-01871-X - IN5020-01876-X

Capillary EX2 .5 IN5020-01872-X IN5020-01873-X - IN5020-01876-X

Capillary EX2 .7 IN5020-01874-X IN5020-01875-X - IN5020-01876-X

1 Capillary EX-nano Connection Kit available IN5020-01881 ($191). LCap EX-nano Splittter also available IN5020-01855 ($511).2 Capillary EX Connection Kit available IN5020-01880 ($133)

Applications available,please enquire.

Capillary and Nano Columns also available for ODS-4, C8-4 and InertSustain™.Please contact us for part numbers and pricing.



Column: Inertsil® PREP-ODS,250mm x 20mm, 10µm

Part Number: IN5020-31123Eluent: CH3OHFlow Rate: 1.0mL/minTemperature: 40°CDetection: RIInj. Volume: 3mL

Ordering Information - Inertsil® PREP Phases

Inertsil® Phase

Column Dimensions (mm)

250 x 61 250 x 10 250 x 20 250 x 30 250 x 50

$993 $1781 $3629 $5343 $14482

PREP-ODS IN5020-02454 IN5020-31113 IN5020-31123 IN5020-31133 IN5020-31153

$993 $1781 $3782 $5477 $15154PREP-C8 IN5020-02464 IN5020-31313 IN5020-31323 IN5020-31333 IN5020-31353

$917 $1546 $2823 $3562 $8620PREP-SIL IN5020-02404 IN5020-31213 IN5020-31223 IN5020-31233 IN5020-31253

Ordering Information - Inertsil PREP Guard Columns

Inertsil® Phase

Column Dimensions (mm) Guard/Prep Set10mm ID1

50 x 10 50 x 20 50 x 30 50 x 50

$807 $1766 $2722 $6302 $2606

PREP-ODS IN5020-31111 IN5020-31121 IN5020-31131 IN5020-31151 IN5020-35112

PREP-C8 IN5020-31311 IN5020-31321 IN5020-31331 IN5020-31351 IN5020-35212

$706 $1430 $2184 $4353 $2236PREP-SIL IN5020-31211 IN5020-31221 IN5020-31231 IN5020-31251 IN5020-35312

1 Various sets of PREP Series columns available. Please enquire. 2 Parameter Determination column

Ordering Information - Inertsil ECONO PREP Phases

Inertsil® Phase

Column Dimensions (mm) Guard Column (mm)

500 x 62 500 x 50 500 x 100 50 x 50 100 x 100

$986 $10779 $33896 $4059 $14717

ECONO PREP-ODS IN5020-36001 IN5020-36055 IN5020-36085 IN5020-36051 IN5020-36081

$648 $7469 $21706 $3797 $10282ECONO PREP-SIL IN5020-36101 IN5020-36155 IN5020-36185 IN5020-36151 IN5020-36181

Purification of essential fattyacid in rapeseed oil

Purification of coptidis rhizomaColumn: Inertsil® PREP-ODS

250mm x 30mm, 10µmPart Number: IN5020-31133Eluent:CH3OH/0.1% CF3COOH = 30/70Flow Rate: 30mL/minTemperature: 40°CDetection: UV 254nmInj. Volume: 2.5mL

Solutes:

1. Coptisine2. Berberine


APPLICATIONS - INERTSIL® (GL Sciences)

Column: Inertsil® ODS-4, 150mm x 4.6mm, 5µm Flow Rate: 1.0mL/minPart Number: IN5020-03945 Detection: UV 290nmMobile Phase: A) CH3CN B) H2O A/B = 15/85, v/v (Mixed by a gradient mixer)Temperature: 40°CInj. Vol: 10µL

9 Pesticides for golf courseColumn: Inertsil® ODS-4, 150mm x 3.0mm, 5µ Part Number: IN5020-03925Eluent: A) CH3OH B) 50mM Soldium citrate (pH 3.5, Citric Acid)

A/B = 45/55, v/v (Mixed by a gradient mixer)Flow Rate: 0.8mL/min Temp: 40°CDetection:UV at 235 nM (GL-7452A PDSA Detector) Inj. Vol: 20µL

Linear fatty acids

Rosmarinic acid

Oxine-copper and caffeine

o-Phenylenediamine Efficient ingredients in cold medicine

Column: Inertsil® ODS-4, 150mm x 4.6mm, 5µmPart Number: IN5020-03945Eluent: A) CH3CN B) 0.1% H3PO4 A/B = 25/75, v/v (Mixed by gradient mixer)Flow Rate: 1.0mL/min Temp: 40°CDetection: UV, 240nm Inj. Vol: 2µL

Analyte:1. Rosmarinic acid(100mg/L in 50% CH3CN)

Column: Inertsil® ODS-4, 150mm x 4.6mm, 5µm Flow Rate: 1.0mL/minPart Number: IN5020-03945 Detection: UV 210nmEluent:A) 0.1% H3PO4 in CH3CNB) 0.1% H3PO4 in H2OA/B = 10/90 - 15min - 90/10 - 10 min - 90/10, v/v(Mixed by a gradient mixer)Temperature: 40°CInj. Vol: 10µL

Column: Inertsil® ODS-4, 250mm x 4.6mm, 5µmPart Number: IN5020-03946Eluent: A) CH3CN B) 20mM H3PO4 in H2O

A/B = 10/90, v/vFlow Rate: 1.0mL/min Temp: 40°CDetection: UV, 254nm Inj. Vol: 2.5µL

Column: Inertsil® ODS-4, 150mm x 4.6mm, 5µmPart Number: IN5020-03945Mobile Phase: A) CH3CN B) 0.1% H3PO4 A/B = 3/97 - 20min- 75/25mixer)Flow Rate: 1.0mL/minTemp: 40°CDetection: UV, 210nmInj. Vol: 10µL

Analyte:

1. Asulam2. Oxine Copper3. Triclopyr4. Mecoprop (MCPP)5. Thiram6. Flazasulfuron7. Halosulfuron methyl’8. Siduron9. Azoxystrobin

Analyte:1. Maleic acid (50mg/L)(Derived chlorpheniramine maleate)2. Acetaminophen (50mg/L)3. Caffein (50mg/L)4. Chlorpheniramine (50mg/L)(Derived chlorpheniramine maleate)5. Ethenzamide (50mg/L)6. Bromovalerylurea (50mg/L7. Apronalide (50mg/L)8. Isopropylantipyrine (50mg/L)9. Ibuprofen (50mg/L)



Column: Inertsil® ODS-3V, 150mm x 4.6mm, 5µm Flow Rate: 1.0mL/minPart Number: IN5020-01801 Detection: UV 240nmEluent: CH3CN/25mM Temperature: 40°C

Acetate buffer (pH 4.3) = 40/60 Inj. Vol: 10µL

18 Artificial colourant compoundsColumn: Inertsil® ODS-3,

150mm x 4.6mm, 4µmPart Number: IN5020-04645Eluent:A) CH3CNB) 10mM NaH2PO4 (pH 6.9 10mM NaH2PO4)Flow Rate: 1.0mL/minTemp: 40°CDetection: UV at 270 nMInj. Vol: 10µL

Vitamin D2 and D3

Alkylbenzene sulfonate

Fungicides

Preservatives Hormones

Column: Inertsil® ODS-3, 250mm x 4.6mm, 5µm Temp: 40°CPart Number: IN5020-01732Eluent: A) o.1M NaCIO4 in CH3CN/H2O = 65/35Flow Rate: 1.0mL/minDetection: FLEx. 221nm EM.284nM

Column: Inertsil® ODS-3, 150mm x 4.6mm, 5µmPart Number: IN5020-01731Eluent: A) CH3CN B) CH3OH

A/B = 75/25, v/vFlow Rate: 1.0mL/minTemperature: 30°CDetection: UV 210nm

Solutes:

1. Vitamin D22. Vitamin D3

Column: Inertsil® ODS-3, 150mm x 4.6mm, 5µm Inj. Vol: 10µLPart Number: IN5020-01731Eluent: A) CH3CN B) CH3OH C) H2O

A/B/C = 5/60/35, v/v/v, 10mM IPCC-12A (pH 2.5 :H3PO4)IPCC-12A: Sodium 1-Dodecylsulfate

Flow Rate: 1.0mL/minTemp: 40°CDetection: FL Ex 285 nm Em 325 nm

Analyte:

1. Acid Yellow No. 42. Acid Red No. 23. Acid Blue No. 24. Acid Red No. 1025. Acid Yellow No. 56. Flavianic Acid Disodium Salt7. Uranine8. Acid Red No. 409. Ponceau R10. Ponceau SX11. Orange I12. Acid Green No. 313. Acid Blue No. 114. Ponceau 3R15. Acid Red No. 316. Azul Blue VX17. Orange II18. Acid Red No. 106

Solutes:

1. Orthophenylphenol(OPP, 0.05mg/L)

2. Thiabendazole(TBZ, 0.01mg/L)

3. Diphenyl(DP, 0.5mg/L)

Column: Inertsil® ODS-3V, 150mm x 4.6mm, 5µm Flow Rate: 1.0mL/minPart Number: IN5020-01801 Detection: UV 230nmEluent: CH3CN/THF/H2O = 35/8/57 Temperature: 40°CInj. Vol: 5µL



Thiourea

Tolylenediisocyanates

Glucose in honey sample

Root extracts

Metal complexes (Phenylacetylacetone) Catechins

Column: Inertsil® ODS-SP, 250mm x 4.6mm, 5µmPart Number: IN5020-02746Eluent: A) CH3CN B) 0.1M Ammonium Acetate buffer (pH 6.2)

A/B = 30/70, v/vFlow Rate: 2.0mL/minTemperature: 40°CDetection: UV 247nmInj. Vol: 10µL

1) 2,6-TDI-2-PP 500µg/L2) 2,4-TDI-2-PP 500µg/L

Solutes:

1. Scopolamine2. Hyoscyamine3. Brucine (I.S.)

Column: Inertsil® ODS-SP, 150mm x 4.6mm, 5µmPart Number: IN5020-02745Eluent: A) CH3OH

B) 10mM NaH2PO4A/B = 10/90 -- (30min) -- 50/50, v/v

Flow Rate: 1.0mL/minTemp: 40°CDetection: UV 280nmInj. Vol: 10µL (each 2.0mg/L)

Solutes:1. Gallocatechin (GC)2. Epigallocatechin (EGC)3. Catechin (C)4. Epigallocatechin Gallate (EGCg)5. Epicatechin (EC)6. Gallocatechin Gallate (GCg)7. Epicatechin Gallate (ECg)8. Catechin Gallate (Cg)

Column: Inertsil® HILIC, 250mm x 4.6mm, 5µm Flow Rate: 1.0mL/minPart Number: IN5020-07736 Detection: UV 245nmEluent: A) CH3CN/25mM Temperature: 40°C

B) H2O Inj. Vol: 10µLA/B = 95/5, v/v (Gradient mixer)

Column: Inertsil® HILIC, 150mm x 4.6mm, 5µm Flow Rate: 0.5mL/minPart Number: IN5020-07715 Detection: RIEluent: Acetonitrile/H2O (85:15, v/v) Temperature: 40°C

Peaks:1. Fructose2. Glucose

Column: Inertsil® ODS-SP, 150mm x 4.6mm, 3µmPart Number: IN5020-02845Eluent: A) CH3CN

B) 50mM KH2PO41% (C2H5) 3N, v/v (pH 3.5 H3PO4)A/B = 10/90, v/v

Flow Rate: 0.9mL/minTemp: 30°CDetection: UV 210nmInj. Vol: 10µL

Column:Inertsil® ODS-SP, 150mm x 3.0mm, 3µmPart Number: IN5020-02825Eluent: CH3OH/H2O = 70/30, v/vFlow Rate: 0.4mL/minTemperature: 40°CDetection: UV 254nmInj. Vol: 10µL

1)Phenylacetylacetone 280mg/L



Polyamines

Vitamin A

Maleic acid and fumaric acid

Phenoxyacetic acid andbenzoic acid

Planar and non-planar compounds Benzene derivatives

Column: Inertsil® ODS-EP, 150mm x 4.6mm, 5µmPart Number: IN5020-02645Eluent: A) CH3CN B) H2O

A/B = 75/25, v/vFlow Rate: 1.0mL/minTemperature: 40°CDetection: UV 325nmInj. Vol: 10µL

Column: Inertsil® Ph-3, 150mm x 4.6mm, 5µmPart Number: IN5020-01302Eluent: A) CH3CN B) H2O A/B = 30/70, w/wFlow Rate: 1.0mL/minTemp: 40°CDetection: UV 254nm

Solutes:1. Acetophenone2. Benzene3. Toluene4. Naphthalene

Column: Inertsil® ODS-EP, 150mm x 4.6mm, 5µmPart Number: IN5020-02645Eluent: 0.1% H3PO4Flow Rate: 1.0mL/minDetection: UV 210nmTemperature: 40°CInj. Vol: 10µL

Analyte::1. Maleic acid (10mg/L)2. Fumaric acid (10mg/L)

Column: Inertsil® ODS-EP, 150mm x 3.0mm, 5µm Flow Rate: 0.4mL/minPart Number: IN5020-02625 Temp: 40°CEluent: A) CH3OH B) 0.1% HCOOH Inj. Vol: 2µL

A/B = 50/50, v/vDetection: PDA 190-400nm, (254nm PDA detector)

Column: Inertsil® ODS-SP, 250mm x 4.6mm, 3µm Flow Rate: 1.0mL/minPart Number: IN5020-02846 Temperature: 40°CGuard Column: IN5020-08510-3µ-ODS-SP Inj. Vol: 10µLEluent: CH3CN/H2O = 65/35, v/vDetection: FL Ex.325nm Em.525nm

Solutes:

1. Putrescine 5.0mg/L2. Cadaverine 5.0mg/L3. Histamine 100mg/L4. 1,8-Diaminooctane (I.S) 10mg/L5. Tyramine 25mg/L6. Spermidine 5.0mg/L

Analyte:

1. 9-cis retinol (10mg/L)2. all-trans retinol (10mg/L)

Column: Inertsil® ODS-P, 150mm x 4.6mm, 5µm Flow Rate: 2.0mL/minPart Number: IN5020-02001 Detection: UV254nmEluent: A) CH3CN B) H2O Temperature: 30°C

A/B = 85/15, v/v

Sample:

1. Phenanthro[3,4-c]phenanthrene (PhPh, Non-planar)2. Tetrabenzonaphthalene (TBN, Non-planar)3. Benzo[a]pyrene (BaP, Planar)

0 10 20Time (min)



Saccharin

Analysis of adrenocortical hormones

Parabens

Proteins Polyethyleneglycol (GFC mode)Column: Inertsil® WP300 Diol, 250mm x 7.6mm, 5µmPart Number: IN5020-05792Eluent: H2OFlow Rate: 1.0mL/minTemp: 40°CDetection: RIInj. Vol: 10µL

Column: Inertsil® Diol, 150mm x 4.6mm, 5µmPart Number: IN5020-05645Eluent: A) CH3CN B) H2O A/B = 20/80, w/wFlow Rate: 1.0mL/minDetection: UV 254nmTemperature: 40°C Solutes:

:1. Ethyl paraben2. n-Propyl paraben3. n-Butyl paraben

Column: Inertsil® NH2, 250mm x 4.6mm, 5µm Flow Rate: 1.0mL/minPart Number: IN5020-05546 Temperature: 40°CEluent: 1% H3PO4/CH3OH = 60/40Inj. Vol: 1µLDetection: UV 210nm

Solutes:

1. Aspartame (0.125mg/mL)2. Stevioside (0.125mg/mL)3. Glycyrrhizic acid (0.125mg/mL)4. Saccharin (0.125mg/mL)

Column: Inertsil® WP300 C8, 150mm x 4.6mm, 5µmPart Number: IN5020-05745Eluent: A) 0.1% TFA

B) 0.1% TFA in CH3CN/H2O = 90/10A/B = 80/20 - 25 min. - 40/60

Temperature: 30°CFlow Rate: 1.0mL/minDetection: UV280nm

Sample:

1. Ribonuclease (0.125mg/mL)2. Insulin (0.040mg/mL)3. Cytochrome (0.100mg/mL)4. Lysozyme (0.129mg/mL)5. BSA (0.260mg/mL)6. Trypsin Inhibitor from Soybean(0.250mg/mL)7. Ovalbumin (0.250mg/mL)

IN5020-01941

Sample:

1. Polyethyleneglycol M.W. 500,0002. Polyethyleneglycol M.W. 20,0003. Polyethyleneglycol M.W. 8,0004. Polyethyleneglycol M.W. 4,0005. Polyethyleneglycol M.W. 300

(All 1mg/mL)

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