Incubator sop

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STANDART OPERATIONAL PROSEDURE OF INCUBATOR

Gandhung Herda .L4411411041Nuraini4411411055Biologi, S1Rombel 2Basic textured: Stainless steel.Power Rating: 230 Volt.Temperature Rangefrom +30 C (however, at least 5 C above ambient) up to +70 C.Inkubator adalah alat pengontrol temperatur, kelembaban, dan kondisi lainnya yang memungkinkan untuk pertumbuhan kultur mikrobiologi.Kelemahan inkubator adalah pada saat penggunaan kelembaban karena seringkali terkontaminasi oleh fungi.IntroductionThe Incubator in Our LaboratoryTrade merk (merek dagang): MemertType : ICP-500 and UNB-200

The Parts of Incubator

Keterangan Panel Indikator pada tipe ICP 600 ENPerbedaan panel indikator pada tipe UNB 200 EN dan ICP 600 EN yang dimiliki laboratorium biologi Unnes hanya terletak pada poin 21, dimana tipe 200 tidak menggunakan kartu/ID Card/Chip Card, sedangkan tipe 600 menggunakan Chip Card dalam pengaturan sistemnya.

Prinsip Kualifikasi Inkubator Menguji penyebaran suhu udara dalam inkubator. (Incubator qualification principle)Prinsip yang pertama Untuk mengetahui penyebaran suhu, maka yang harus dilakukan :

mempelajari struktur/bentuk inkubator, letak sumber panasnya, sensor, dan sirkulasi udaranya (perhatikan letak exhaust, bila ada)gunakan thermocouple dan thermorecorder yang sudah terkalibrasi untuk merekam dan memantau suhu dalam inkubator selama waktu tertentu (biasanya berdasarkan waktu pemakaian)12Pemantauan suhu setiap titik pengukuran saat inkubator dibuka dan ditutup kembali.Prinsip yang Kedua Faktor yang mempengaruhi perubahan suhu inkubator yaitu udara luar yang masuk akibat dibukanya pintu inkubatorHasil yang baik adalah bila inkubator mampu menjaga stabilitas suhunya saat pintu dibuka dan ditutup kembali.Antisipasi bila terjadi pemadaman listrik (power failure).Prinsip yang Ketiga Inkubator dimatikan beberapa saat sambil dipantau penurunan suhunya, lalu dinyalakan kembali dan dipantau suhunya hingga mencapai suhu setting.Hasil kualifikasi yang diharapkan adalah inkubator mampu menjaga suhunya pada waktu yang maksimal saat listrik padam, hingga generator pabrik dinyalakan, dan penyesuaian suhu saat inkubator dihidupkan tidak memerlukan waktu lama.Pengujian kualitas inkubator harus dilakukan pada beberapa kondisi: kosong, terisi penuh, dan terisi sebagian.Prinsip yang Keempat Hasil yang diinginkan adalah suhu tetap terdistribusi rata sesuai dengan setting dan display saat kondisi kosong, penuh, maupun terisi sebagian.Cara PenggunaanSambil menekan tombol set, geser panel power ke kanan untuk mengatur suhu sesuai keinginanLepaskan tombol set

Cara PenggunaanHubungkan kabel power ke stop kontak.Putar tombol power ke arah kiri (lampu power hijau menyala).Atur suhu dalam incubator dengan menekan tombol set.Sambil menekan tombol set, putarlah tombol di sebeklah kanan atas tombol set hingga mnencapai suhu yang di inginkan.Setelah suhu yang diinginkan selesai diatur, lepaskan tombol set.Inkubator akan menyesuaikan settingan suhu secara otomatis setelah beberapa menit.

Prosedur Tetap untuk Perawatan Inkubator

Bersihkan bagian dalam dan rak incubator setiap bulan dengan desinfektan.Catat suhu incubator pada kartu setiap hari sebelum mulai bekerja.Perbedaan suhu yang melebihi , pengatur suhu perlu disetel kembali.Suhu yang masih dapat diterima adalah dari suhu yang diinginkan.

(Fixed Prosedure to Incubator Protection)SOP dari produk Memmert dapat dilihat di bawah ini atau di sini

1. ScopeThis Standard Operating Procedure (SOP) applies to the staff and students using and/or maintaining the Memmert Universal Oven in the laboratories of the Pharmacy Department, University of Malta.2. ObjectiveTo give an account of the procedure for the installation, operation and maintenance of the Memmert Universal Oven.

3. Definitions3.1 Air slide: Fresh air supply control. Levels range from 0 to 6, zero giving free internal convection circulation whilst six giving maximum entry of fresh air. Ideally this should be set at intermediate positions to permit selection of the appropriate mixing ratio since a large proportion of fresh air may not always allow the selected setpoint temperature to be reached.3.2 Main switch: On and Off or Timer Operation.3.3 Signal lamps:3.3.1 Green: Ready for operation3.3.2 Red: Over temperature protection or fault3.3.3 Orange: Heating3.4 Set point button: Push-button to allow the temperature knob to operate.3.5 LCD display: Digitally displays setpoint and the actual indication of the thermostat.3.6 Screw lock: Locks temperature set point to prevent undesirable change in setting.3.7 Knob (black): Temperature knob used to select the desired temperature setpoint.3.8 Knob (red): Over-temperature protection adjustment knob to prevent overheating.4. Responsibility 4.1 The members of the Department of Pharmacy (staff and students) are responsible for following this SOP. 4.2 The designated Laboratory Officer or Laboratory Assistant are responsible for ensuring that this SOP is followed.

5. Procedure 5.1 Installation 5.1.1 Accurately position oven horizontally, using a spirit level if necessary. 5.1.2 Allow at least a distance of 15cm between the back of the oven and the wall. 5.1.3 Alow a distance of not less than 20cm between the ceiling and the oven. 5.1.4 Adjust oven door if required. 5.2 Operation5.2.1 Set the main switch on.5.2.2 Depress the set point button continuously. Subsequently manipulate the temperature knob to the desired temperature. At this stage, the digital temperature indication switches to set-point selection and this is indicated by a blinking point in the display.5.2.3 Move the temperature knob quickly to adjust set-point in large steps.5.2.4 Move the temperature knob slowly to adjust set-point in single steps.5.2.5 Lock the scale with the screw lock to prevent unintentional changes.5.2.6 Set the adjustable overheat controller, with the red knob, above the selected working temperature by an appropriate amount (eg. by 5C).5.2.7 Wait until a steady temperature is reached, i.e. when the desired temperature is available on the LCD display for a period of at least 15 minutes, to insert items into the oven using the different shelving options available.5.2.8 Move the red knob down until the oven does not operate when handling heat senstive loads to protect them against over-temperature. Perform this set-point reduction very slowly, step by step by one digit slightly above the actual steady temperature.5.3 Maintenance5.3.1 Lubricate the moving parts on the door (hinges and door lock) with a thin silicone grease once a year. Lubricate 4 times a year if oven is continuously being used.5.3.2 Adjust the door at the hinges and/or the locking plate area to ensure that the door closes properly.5.3.3 Diagram of Door Hinge (Left) and Locking Plate (Right)5.3.3.1 Adjust door hinges by releasing screw 16 and then, by means of a screwdriver, turn part 17 in the direction of the arrow displayed. Also, adjust the upper part of the door hinge (part 18) in the direction of the arrow byreleasing the 2 screws present at the top and bottom of the door.5.3.3.2 Adjust locking plate in the direction of the arrow by releasing screw 19.

5.4 Flow Charts5.4.1 Installation

5.4.2 Operation

5.4.3 Maintenance

6. Precautions6.1 Be aware of the physical and chemical properties of the load (eg. Ignition Temperature) to prevent considerable damages to load, oven and its surroundings.6.2 For heat sensitive and valuable items:6.2.1 Oven is to be continually observed and monitored by an operator.6.2.2 Oven is to be fitted with over temperature protection to DIN Class 2 (adjustable temperature limiter) or Class 3.1 (adjustable temperature monitor).6.3 Position the instrument horizontally to ensure that optimum control and thermal conditions are maintained.6.4 Take special care when working at high temperatures. Outside case temperature may be high.6.5 Do not use the oven for drying or heating items giving off vapours which are inflammable when mixed with air.6.6 Do not pack load tightly to ensure free air circulation.6.7 Do not place anything on the bottom, against the sides or underneath the top of the working space (heating ribs).6.8 Use a drip tray for wet loads.6.9 Close the flap of the oven completely or else the oven may take a very long time to reach the selected temperature.7. References Memmert GmbH+Co. KG.Operating Instructions for Electronically Controlled Ovens UE/BE/ULE 400-800. Germany: Memmert GmbH+Co. KG; 1990. 8. Appendices N/A 9. Revision History

Lets see the video ^^MemmertShel LabIta : perbedaan inkubator panel dan kartuTyas : perbedaan antara memmert dan shel lab. Dan perbedaan dengan yang ada di lab mikro ?Kamila : apakah sample padat cair dapat diinkubasi ?