Methods

MethodsMaterialsPlasmodium Berghei from National Institute of Immunology, New Delhi, India

Methods:Evaluation of Oral Bioavailability of Nanotized Curcumin

C57BL/6 mice2 Groups of 6 micesNanotized curcumin20 mg/kgbb orallyNative curcumin20 mg/kgbb orallyBlood samples 0,2 ml at 0, 2, 5, 10, 15, 20, 25, 30 hours after+Normal saline 0,2 ml after collecting every blood samplecentrifugeanalysisCmax & Tmax

Methods:Dose Kinetics Determination on C57BL/6 Mice Infected with P. BergeiC57BL/6 miceP. Berghei 2,5x106 ivOral Chloroquine(control + )Native Curcumin 10, 20, 40 mg/kgbbNanotized Curcumin 10, 20, 40 mg/kgbbNormal saline(control - )Parasitemia levels checked every four daysN=6biweeklyMethods:Evaluation of Hepatoprotective and Nephroprotective Activity of Nanotized CurcuminNormal (uninfected) C57BL/6 miceN=6C57BL/6 miceP. Berghei 2,5x106 ivOral Chloroquine(control + )Native Curcumin 20 mg/kgbbNanotized Curcumin 20 mg/kgbbuntreatedbiweeklyBlood samples1 monthALP, Bilirubin, SGOT, SGPT, Urea, CreatinineResult

Evaluation of Oral Bioavailability of Nanotized Curcumin

Dose Kinetics Determination on C57BL/6 Mice Infected with P. Berghei

Evaluation of Hepatoprotective and Nephroprotective Activity of Nanotized Curcumin