Improving RAST annotations within RAST
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www.rast.nmpdr.org Improving RAST annotations within RAST Veronika Vonstein Fellowship for Interpretation of Genomes December 2008
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Improving RAST annotations within RAST. Veronika Vonstein Fellowship for Interpretation of Genomes December 2008. Your genome after RAST. SEED genome in RAST. Use Function based c omparison t ool. Choose genome to compare to. - PowerPoint PPT Presentation
Transcript of Improving RAST annotations within RAST
www.rast.nmpdr.org
Improving RAST annotations within RAST
Veronika VonsteinFellowship for Interpretation of Genomes
December 2008
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Your genome after RAST
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SEED genome in RAST
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Use Function based comparison tool
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Choose genome to compare to
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Compare Metabolic Reconstruction of your genome (A) and the SEED
genome (B)
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1. Case: Candidate present but Function different
But to eliminate the risk of annotating a paralog
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Compare Regions
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Annotate Chromosomal Clusters
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Annotate set 1
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Set 1 done continue
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2. Case: The real missing
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3. Case: Missed gene call
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Your genome after RAST
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Your improved genome
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Summary
• Re-annotated 490 gene functions• Increased the number of pegs in
subsystems from 36% to 50%• This added 60 subsystems to the
metabolic reconstruction of your genome• Possible to add missed genes, annotate
them and connect them to subsystems
