Impedance Spectroscopy for Toxicity Testing Martin Dominguez Cronus Technologies Ltd.
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Transcript of Impedance Spectroscopy for Toxicity Testing Martin Dominguez Cronus Technologies Ltd.
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Impedance Spectroscopy for Toxicity Testing
Martin DominguezCronus Technologies Ltd.
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Barrier Forming TissueEpithelial and endothelial cell layers form selectively permeable barriers
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The ConceptGrowing cell layers on permeable membranes
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The ConceptMeasuring the electrical impedance of epithelial and endothelial cell layers under physiological conditions
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Understanding Impedance SpectraContributions stemming from CPE and RMED
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Understanding Impedance SpectraThe formation of a plateau at mid-range frequencies is attributable to the cell layer’s resistance TER and capacitance CCL
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Understanding Impedance SpectraAn increase in the resistance TER shifts the plateau upwards
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Understanding Impedance SpectraAn increase in the capacitance CCL narrows the plateau
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Time-Lapse Impedance Spectroscopy
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The Setup
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The Setup
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cellZscopeAutomated cell monitoring device
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Monitoring of MDCK-II CellsFormation of a differentiated cell monolayer with established cell-cell junctions
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cellZscope vs. „Chopstick“ ElectrodesComparing TER values measured on MDCK-II cell layers
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Long Term Monitoring of Caco-2 CellsComparing growth dynamics of different cell lines
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TER
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Control1mM EGTA2mM EGTA4mM EGTA
EGTA
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EDTA Treatment of MDCK-II CellsEffects on the tight junction network
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MBCD Treatment of MDCK-II CellsDose dependent response in TER and Ccl to cholesterol depletion
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Testing the Toxicity of NanoparticlesExposure of MDCK cells to different types of particles
+ Triton X100
ControlControl
Carbon Black*
Carbon Black*
ZrO2*
ZrO2*
ZnO - NP*
ZnO - NP*
+ Particles
* 10 µg/cm²
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Data courtesy of A. Kroll, J. Schnekenburger, University of Münster, Germany
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Testing the Toxicity of NanoparticlesExposure of NRK52E cells to ZnO
ControlControl
ZnO*
ZnO*
ZnO*
ZnO NP*
ZnO NP*
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+ ZnO + Triton X100
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Data courtesy of A. Kroll, J. Schnekenburger, University of Münster, Germany
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cellZscopeAutomated cell monitoring device