Impact of nano formulation in horticultural crop pest management
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Transcript of Impact of nano formulation in horticultural crop pest management
BIGGEST REVOLUTION BY
THE SMALLEST SCIENCE
Welcome toNANO WORLD
Impact of Nano formulation in Horticultural crop Pest
Management V. G O W T H A M
2 0 1 6 6 0 0 5 0 4A G R L . E N T O M O L O G Y
TA M I L N A D U A G R I C U LT U R A L U N I V E R S I T Y
Nanotechnology…?
“Nanotechnology is the art and science of manuplating matter at nano scale’’
“Nanotechnology refers to controlling, building, and restructuring materials and devices on the scale of atoms and molecules”.
A nanometer (nm) is one-billionth of a meter.
Nanopesticides
Nano-scale either active ingredients with a particle size of 100 nm or less
Formulation of a pesticides Nano emulsionNano suspension Nano encapsulationNanoparticles
Nano Emulsion
Consist of lipid or polymeric vesicles or particles
Size 20-200nm
Larger surface area, slower release
Non sedimentation or creaming
Development of an insecticidal nanoemulsion with Manilkara subsericea (Sapotaceae) extract
Fernandes et al. 2014Journal of Nanobiotechnology 2014,
12:22
Hexane-soluble fraction from ethanolic crude extract from fruits of Manilkara subsericea and its triterpenes were considered active against a cotton pest (Dysdercus peruvianus).
poor water solubility, including triterpenes
A nano emulsion prepared 5% of oil (octyldodecyl myristate), 5% of surfactants (sorbitan
monooleate/polysorbate 80), 5% of apolar fraction from M. subsericea 85% of water.
Nano EncapsulationEncapsulation packaging the nano-scale
active ingredient within a kind of tiny “ envelope or shell”
Nanotechnology enables us to manipulate the properties of the outer shell of the capsule.
Uses
Protection
Increase solubility
Reduce the contact of active ingredients with agriculture workers
Environments reducing run off rates
Mohdzobir et al., 2011
Efficient Management of Fruit Pests by Pheromone Nanogels
Deepa Bhagat et al.,Scientific Reports | 3 : 1294 | DOI: 10.1038/srep012942013
Pheromones
• Nanogel prepared using methyl eugenol (ME)+low-molecular mass gelator
• Very stable at ambient condition• works well in rainy season• Last for month
(Deepa Bhagat,2013)
Slope magnitude comparison
(Deepa Bhagat,2013)
(Deepa Bhagat,2013)
30 days after storage
Fruit flies attracted towards the thin film nanogel
1 2
3 4Nanogel
Fruit fly counting
Establishment
(Deepa Bhagat,2013)
Field experiment
Num
ber o
f cat
ches
Number of days (Deepa Bhagat,2013)
Number of days of catches for the ME and Nano ME gel
Nano suspension
Submicron colloidal dispersion of pure active compounds typically range from 50-500nm.
Improvement of efficacy due to higher surface area.
Higher solubility, higher mobility.
Induction of systematic acivity due to smaller particle size.
Nanoparticles
Solid core or matrix, usually composed of metallic atom
Material used for the fabrication Metal oxide certain and silicates, magnetic material.
Biocompatible Chitosan Nanoparticles Incorporated Pesticidal Protein Beauvericin (CSNp-BV) Preparation for the Improved Pesticidal Activity Against Major Groundnut
Defoliator Spodoptera Litura (Fab.) (Lepidoptera; Noctuidae)
Arvind Bharani et al.,International Journal of ChemTech Research,2014
Cumulative mortality (%) of S.litura treated with free beauvericin (F-BV) and chitosan nanoparticles incorporated beauvericin (CSNp-BV)
(Arvind Bharani, 2014)
Effect of free beauvericin (F-BV) and chitosan nanoparticles incorporated beauvericin (CSNp-BV) on pupal and adult emergence
(Arvind Bharani, 2014)
DNA-tagged nano gold: A new tool for the control of the armyworm, Spodoptera
litura Fab. (Lepidoptera: Noctuidae)
Chakravarthy et al.,African Journal of Biotechnology Vol. 11(38), pp. 9295-9301, 10
May, 2012
4th days after treatment
5th days after treatment 6th days after treatment
3rd days after treatment
7th days after treatment
control
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