Impact of Manure & Nitrogenon the Amount of Antibiotic ... · Antibiotic resistance genes (ARGs)...
1
Antibiotic resistance genes (ARGs) are a concern for human and ecosystem health. Agricultural manure is a source of resistant bacteria with the potential to transfer ARGs by food or contaminate the environment. It is important to study ARGs to have a better understanding of human health, diseases, and the causes of them. We thank Virginia L. Jin and Marty R. Schmer for maintaining the plots and cooperating in collection of the samples. We also thank all the people in lab: Sue Siragusa, Morgan Meyers, Brandon Nguyn, Alexis Overman, Elizabeth VanWormer, and Vanessa Williams for their support. This program was supported by Grant Program no. 2017-67032-26018 from the USDA National Institute of Food and Agriculture and USDA-ARS NP212, Soil and Air. Impact of Manure & Nitrogen on the Amount of Antibiotic Resistance Genes in Soil Does animal manure impact the amount of antibiotic resistance genes in soil? Introduction To evaluate selected Antibiotic resistance genes (ARGs) in manured soil. In this research, the impacts of manure and different amounts of nitrogen on ARGs was studied. Agriculture Research Services Nazanin Ghetmiri 1 , Lisa M. Durso 2 , Virginia L. Jin 2 , Marty R. Schmer 2 University of California Los Angeles 1 , USDA-ARS 2 • Based on the results, long term manure application does not increase the ARGs in soil. • Higher amounts of nitrogen does not impact the amount of ARGs in soil. • 16s microbial community assays are underway. • Add in 14 assay of tetracycline to collect information on genes. No difference on amount of gene copies was observed for any of these targets based on manure or nitrogen treatment: • sul1: sulfonamide- important in environment • INTI1: Integrase- helps resistance genes to move • ermB:Macrolide- important in human and animal • ctx-m-32: Beta-lactamase- important in human medicine Culture based assays indicate no difference on amount of tetracycline resistant bacteria and sensitive antibacterial based on manure or nitrogen treatment. • Tet: Tetracycline- antibiotics Objective Methods Collection of Samples, n=48 Replication= 4 Nitrogen rate= N: 125 kg N ha-1, N2: 200 kg N ha -1 Precipitation= 100% Irrigation DNA Isolation Quantitative Polymerase Chain Reaction Culture no tetracycline with tetracycline Results Conclusion Future Work Acknowledgements 1.00E+00 1.00E+01 1.00E+02 1.00E+03 1.00E+04 1.00E+05 1.00E+06 Sul 1 INTI 1 erm B ctx-m Gene Copies/Gram of Dry Soil Antibiotic Resistance Genes Quantitative Polymerase Chain Reaction Results no manure+N1 no manure+N2 manure+N1 manure+N2 1 10 100 1000 10000 no tet+N1 no tet+N2 tet+N1 tet+N2 Colony Forming Unit/Gram Different Treatment Culture Results no manure manure
Transcript of Impact of Manure & Nitrogenon the Amount of Antibiotic ... · Antibiotic resistance genes (ARGs)...
Antibiotic resistance genes (ARGs) are a concern for human and ecosystem health. Agricultural manure is a source of resistant bacteria with the potential to transfer ARGs by food or contaminate the environment. It is important to study ARGs to have a better understanding of human health, diseases, and the causes of them.
We thank Virginia L. Jin and Marty R. Schmer for maintaining the plots and cooperating in collection of the samples. We also thank all the people in lab: Sue Siragusa, Morgan Meyers, Brandon Nguyn, Alexis Overman, Elizabeth VanWormer, and Vanessa Williams for their support. This program was supported by Grant Program no. 2017-67032-26018 from the USDA National Institute of Food and Agricultureand USDA-ARS NP212, Soil and Air.
Impact of Manure & Nitrogen on the Amount of Antibiotic Resistance Genes in Soil
Does animal manure impact the amount of antibiotic resistance genes
in soil?
Introduction
To evaluate selected Antibiotic resistance genes (ARGs) in manured soil. In this research, the impacts of manure and different amounts of nitrogen on ARGs was studied.
Agriculture Research Services
Nazanin Ghetmiri1, Lisa M. Durso2, Virginia L. Jin2, Marty R. Schmer2
University of California Los Angeles1, USDA-ARS2
• Based on the results, long term manure application does not increase the ARGs in soil. • Higher amounts of nitrogen does not impact the amount of ARGs in soil.
• 16s microbial community assays are underway. • Add in 14 assay of tetracycline to collect information on genes.
No difference on amount of gene copies was observed for any of these targets based on manure or nitrogen treatment:• sul1: sulfonamide- important in environment• INTI1: Integrase- helps resistance genes to move• ermB:Macrolide- important in human and animal• ctx-m-32: Beta-lactamase- important in human medicine
Culture based assays indicate no difference on amount of tetracycline resistant bacteria and sensitive antibacterial based on manure or nitrogen treatment. • Tet: Tetracycline- antibiotics
Objective
Methods
Collection of Samples, n=48Replication= 4Nitrogen rate= N: 125 kg N ha-1, N2: 200 kg N ha-1
Precipitation= 100% Irrigation
DNA IsolationQuantitative Polymerase
Chain Reaction
Culture
no tetracycline with tetracycline
Results
Conclusion
Future Work
Acknowledgements
1.00E+00
1.00E+01
1.00E+02
1.00E+03
1.00E+04
1.00E+05
1.00E+06
Sul 1 INTI 1 erm B ctx-m
Gen
e C
opie
s/G
ram
of D
ry S
oil
Antibiotic Resistance Genes
Quantitative Polymerase Chain Reaction Results
no manure+N1 no manure+N2 manure+N1 manure+N2
1
10
100
1000
10000
no tet+N1 no tet+N2 tet+N1 tet+N2
Col
ony
Form
ing
Uni
t/Gra
m
Different Treatment
Culture Results
no manure manure
![public static void main(String [] args) - katie.mtech.edu · public static void main(String [] args) { String product = args[0]; ... •Manual: cast or using a method 24 expression](https://static.fdocuments.net/doc/165x107/5baa6eb309d3f221798c7bf7/public-static-void-mainstring-args-katiemtechedu-public-static-void.jpg)
