ImmunologyChapter 2, Lecture 1

• Richard L. Myers, Ph.D.

• Department of Biology

• Southwest Missouri State

• Temple Hall 227

• Telephone: 417-836-5307

• Email: rlm967f@mail.smsu.edu

• Homepage: http://creative.smsu.edu/biology/myers/index.html

• TopClass: http://creative.smsu.edu

Horses may be used when a large amount of antiserum is needed

Primates may be used in research related to vaccine development

Experimental Systems

• Experimental animal models include mice– easy to handle– genetically well characterized– rapid breeding cycle

• Inbred strains (syngeneic)– repeated inbreeding between litter mates– C3H– C57BL/6

C57 black mouse, the experimental animal of choice

Comments on the use of animals in research and teaching

Ethical issue?

Science, 14 August 1998

Adoptive-transfer systems

• Used to eliminate the immune response of a syngeneic host– eliminate with x-ray (650-750 rads)– then reconstitute with other lymphocytes

• X-ray will lead to death of the mouse unless reconstitution is accomplished

Experimental Systems

• Scid mice– must be raised in a germ-free environment– can accept transplanted bone marrow cells

• Cell culture systems– primary lymphoid cell cultures– cloned lymphoid cell lines– hybrid lymphoid cell lines

Cell culture laboratory

SCID mice grafted with human scalp and treated with X-ray to

study hair loss

Fetal tissue research

Ethical issue?

Some claim that FTT will exploit women, yield increasedabortions, and benefit doctors financially from the tissue.Highlighting one particular fear, it is suggested that womenwill intentionally become pregnant to provide tissue forscience. "Baby harvesting exploits pregnant womenbecause the preferred procedure for some tissue harvestingleaves the child intact as much as possible and requiressignificant change in the abortion procedure, which maymake it more dangerous to women," said an individualopposed to FTT.

Assignment

• Read Chapter 1 (Overview) for information and review only

• Read Chapter 2, Experimental Systems

• Review question 12 (pg 45)