ILA vaksine – forskningsnytt...ILA vaksine – forskningsnytt Espen Rimstad, NMBU-...
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ILA vaksine – forskningsnytt
Espen Rimstad, NMBU- Veterinærhøgskolen
Espen Rimstad, NMBU
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• Efficient • Induce disease protection • Reduce/stop shedding • Long-lasting immunity • Administrated once
• Safe • No/little side effects • No/little environmental effects • No effect for consumer
• Low cost? • Easy to administrate
• Injectable versus oral or dip?
Ultimate fish vaccine – requirements?
Espen Rimstad, NMBU
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• ViVaFish is a platform project funded by Research Council of Norway (RCN)
Primary institutions
National and international partners
Industrial partners
VIVAFISH Platform for Fish Virus Vaccines
Espen Rimstad, NMBU
http://www.google.no/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&docid=TiRAql-_cQMhMM&tbnid=yrhGdmMva--FDM:&ved=0CAUQjRw&url=http://www.haapihavet.com/program/bedriftsstand/&ei=BUt8U7W2J5T44QTR_IHABw&bvm=bv.67229260,d.bGE&psig=AFQjCNHlphEEatzJRjD4HhwFXq6QzKwL4w&ust=1400740997131426http://www.google.no/url?sa=i&source=images&cd=&cad=rja&uact=8&docid=bZd0VRXnvVewcM&tbnid=gYdaGtFxNYA-bM:&ved=0CAgQjRw&url=http://site.uit.no/fornybar/&ei=lkp8U9vbJeeO4gTbnIGgAQ&psig=AFQjCNHS10fKcYqJVEf2AEiJVul_TdXEfw&ust=1400740886695688http://www.google.no/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&docid=r9nLfYMyS7GtJM&tbnid=8cDi-kU3XXsKdM:&ved=0CAUQjRw&url=http://www.sas.upenn.edu/~marcmere/&ei=IFB8U-WWB9OL4gSkiIHABg&bvm=bv.67229260,d.bGE&psig=AFQjCNEAC8f0-QelCnDFGdjn5aIUhGpNMQ&ust=1400742301779447http://www.google.no/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&docid=rZq3oodRLA2wZM&tbnid=ONlt0t9PTsTFgM:&ved=0CAUQjRw&url=http://www.sjaweb.org/apps/pages/index.jsp?uREC_ID%3D181507%26type%3Dd%26pREC_ID%3D385735&ei=lFB8U-PzGafg4QTCiIHACw&psig=AFQjCNFIxP9LOiGemZOEBLkxcCORiprwaA&ust=1400742375772096
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Whole virus and subunit preparations
Vectorized, propagation-defective
Live attenuated virus
VIVAFISH Platform for Fish Virus Vaccines
Vaccine concepts
Espen Rimstad, NMBU
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Link between innate and adaptive immune
responses
Analyze adaptive CTL responses
B-cell responses involved in long term protection
VIVAFISH Platform for Fish Virus Vaccines
Immunology of long term protection
Espen Rimstad, NMBU
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Optimization of delivery systems
Assays for long-lasting antiviral immunity
RNA seq / Array
VIVAFISH Platform for Fish Virus Vaccines
Espen Rimstad, NMBU
http://www.google.no/url?sa=i&rct=j&q=&esrc=s&frm=1&source=images&cd=&cad=rja&uact=8&docid=Zmjqewiuf-j6VM&tbnid=NaAWIE0BO0dUxM:&ved=0CAUQjRw&url=http://www.unil.ch/ib/page81028.html&ei=Wc1XU_ytF-uAywOEk4DoDw&bvm=bv.65177938,d.bGQ&psig=AFQjCNEVCFVAKAl-DwwltidMJZUnmvUvzw&ust=1398349141003370
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Improved prototypes for vaccines
Vaccine delivery
response monitoring
Immunology of long term protection
Vaccine concepts
VIVAFISH Platform for Fish Virus Vaccines
Efficacy Safety In target species Dissemination Reversion Latency Ecotoxicity Target versus non-target species Host range. Establishment in the environment Potential for recombination
Espen Rimstad, NMBU
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• Robertsen B, Chang CJ, Bratland L. IFN-adjuvanted DNA vaccine against infectious salmon anemia virus: Antibody kinetics and longevity of IFN expression. Fish Shellfish Immunol. 2016 Jul;54:328-32. doi: 10.1016/j.fsi.2016.04.027. PubMed PMID: 27108379.
• Caruffo M, Maturana C, Kambalapally S, Larenas J, Tobar JA. Protective oral vaccination against infectious salmon anaemia virus in Salmo salar. Fish Shellfish Immunol. 2016 Jul;54:54-9. doi: 10.1016/j.fsi.2016.03.009. PubMed PMID: 26994669.
• Tobar I, Arancibia S, Torres C, Vera V, Soto P, Carrasco C, Alvarado M, Neira E, Arcos S, Tobar JA. Successive Oral Immunizations Against Piscirickettsia Salmonis and Infectious Salmon Anemia Virus are Required to Maintain a Long-Term Protection in Farmed Salmonids. Front Immunol. 2015 May 27;6:244. doi: 10.3389/fimmu.2015.00244. PubMed PMID: 26074916; PubMed Central PMCID: PMC4445318.
• Chang CJ, Robertsen C, Sun B, Robertsen B. Protection of Atlantic salmon against virus infection by intramuscular injection of IFNc expression plasmid. Vaccine. 2014 Aug 6;32(36):4695-702. doi: 10.1016/j.vaccine.2014.05.059. PubMed PMID: 24996122.
• Wolf A, Hodneland K, Frost P, Hoeijmakers M, Rimstad E. Salmonid alphavirus-based replicon vaccine against infectious salmon anemia (ISA): impact of immunization route and interactions of the replicon vector. Fish Shellfish Immunol. 2014 Feb;36(2):383-92. doi: 10.1016/j.fsi.2013.12.018. PubMed PMID: 24374059.
• Wolf A, Hodneland K, Frost P, Braaen S, Rimstad E. A hemagglutinin-esterase-expressing salmonid alphavirus replicon protects Atlantic salmon (Salmo salar) against infectious salmon anemia (ISA). Vaccine. 2013 Jan 11;31(4):661-9. doi: 10.1016/j.vaccine.2012.11.045. PubMed PMID: 23200939
ILA vaksine
Espen Rimstad, NMBU
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Espen Rimstad, NMBU
VIVAFISH Platform for Fish Virus Vaccines
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Espen Rimstad, NMBU
VIVAFISH Platform for Fish Virus Vaccines
Chang et al 2014, Robertsen et al 2016
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Pancreas disease
virus is an Alphavirus
Espen Rimstad, NMBU
VIVAFISH The use of PD virus as a vaccine vector
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(Amplification+ dsRNA) CMV/T7 promoters
HH 5’UTR nSP1-2-3-4 Gene of interest 3’ UTR pA T7t
pnSP -SDV
J
IM injection to fish
nSP1234
Antigen expressed intracellularly
Ag presentation
MHC
Ab (IgM)
Cellular response T cyto.
Antigen
Espen Rimstad, NMBU
VIVAFISH The use of PD virus as a vaccine vector
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(Amplification+ dsRNA) CMV/T7 promoters
HH 5’UTR nSP1-2-3-4 Gene of interest 3’ UTR pA T7t
pnSP -SDV
J
IM injection to fish
nSP1234
Antigen expressed intracellularly
Ag presentation
MHC
Ab (IgM)
Cellular response T cyto.
Antigen
Espen Rimstad, NMBU
VIVAFISH The use of PD virus as a vaccine vector
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Espen Rimstad, NMBU
VIVAFISH Platform for Fish Virus Vaccines
Infeksiøs lakseanemi - ILA Wolf et al 2013, 2014
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Espen Rimstad, NMBU
VIVAFISH Platform for Fish Virus Vaccines
Pankreas sykdom - PD Hikke et al 2014
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Oral vaccines
Espen Rimstad, NMBU
Tobar et al 2015 –CentroVet (Chile)
Oral booster etter injeksjonsvaksiner gir økt mengde spesifikk IgM
Caruffo et al 2016,–CentroVet (Chile)
Oral vaksine alene gir en viss beskyttelse dersom den er pakket inn i spesielle mikrocapsuler.
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• ISAV is a relative to Tilapia Lake virus.
Espen Rimstad, NMBU
ISA as a model?
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