IL-2 as the adjuvant in FMD vaccine Chunxia su , ph.D. Ningxia Medical University Yinchuan ,...
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Transcript of IL-2 as the adjuvant in FMD vaccine Chunxia su , ph.D. Ningxia Medical University Yinchuan ,...
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IL-2 as the adjuvant in FMD vaccine
Chunxia su, ph.D.
Ningxia Medical UniversityYinchuan, China
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Overview of FMD
Foot-and-mouth disease (FMD) is a severe, highly
contagious and economically devastating viral
disease worldwide.
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Overview of FMD vaccine
• Inactivated antigen vaccines have been available
since the early 1900s.
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Overview of FMD vaccine
Shortcomings of traditional vaccines for
FMD : • First, there are so many different strains of the FMD
virus;
• Second, traditional vaccines involve live FMD virus;
• Third, traditional vaccines can not differentiate
infected from vaccinated animals.
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Overview of FMD vaccine
• The need for better foot-and-mouth disease (FMD) vaccines is not new.
• subunit vaccine• live vector vaccine• Nucleic acid vaccines• Novel attenuated vaccine• Synthetic peptide vaccine
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Overview of FMD vaccine
• Humoral immune response was considered to be an
important defence mechanism in FMD infection.
• In recent past, there has been an increasing
evidence of the importance of T cell mediated
immunity in FMD.
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Overview of FMD vaccine
• In recent years, special attention has been
paid to cytokines as FMD vaccine adjuvants.
• IFNα,β,γ;IL-2,IL-6,IL-9,IL-18,IL-15,TNFα,IL-1β
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IL-2 is a very popular adjuvant
• IL-2 is one of the most widely used adjuvants
for vaccination to stimulate the proliferation
and activation of various immune effector
cells such as T cells, NK cells, B cells, and
macrophages.
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IL-2 is a very popular adjuvant
• IL-2 enhanced the immunogenicity of the FMD
DNA vaccine candidate.
H.-T. Wong et al. Vaccine,2002
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IL-2 is a very popular adjuvant
• IL-2 was able to enhance the specific immune response against FMD virus type O, A22 and Asia 1.
S. Yadav et al. Vaccine,2005
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IL-2 is a very popular adjuvant
IL-2 is important in many types of T effector
cell differentiation.
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• T follicular helper cells (Tfh), a new specialized subset of CD4+ helper T cells, providing help to B cells.
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Ma C S et al. J Exp Med 2012;209:1241-1253© 2012 Ma et al.
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Su C,et al. PLoS One , 2013
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Su C,et al. PLoS One , 2013
PNA+
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Su C,et al. PLoS One , 2013
rAd5VP1 induced the secretion of IL-21 and the
expression of Bcl-6 mRNA.
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Can IL-2 as an adjuvant for FMD vaccine candidate promote the generation of T follicular helper cells?
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PNA+
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summary
• IL-2 as an adjuvant for an adenovirus-vectored FMD vaccine candidate through the generation of Th1, Th2 as well as T follicular helper cells.
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acknowledgement
Xiangguo Duan, Ph.D.Guanxian Xu, Ph.D.Jie Zheng, graduate studentFeng Wang, graduate student
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Thanks for your patient !
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Liao et al, Immunity,2013
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Thomas R. Malek, Wasif N. Khan. Immunity 2012
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Oestreich et al. Nat Immunol, 2013