IgA anti-tTG antibodies in children with severe short stature without gastrointestinal...
-
Upload
faraz-ahmad -
Category
Documents
-
view
214 -
download
1
Transcript of IgA anti-tTG antibodies in children with severe short stature without gastrointestinal...
LETTER
IgA anti-tTG antibodies in children with severe short staturewithout gastrointestinal manifestations
Faraz Ahmad & Seema Alam & Indu Shukla &
Rana Sherwani & Syed Manazir Ali
Received: 24 October 2010 /Accepted: 18 December 2011 /Published online: 4 February 2012# Indian Society of Gastroenterology 2012
Atypical celiac disease (CD) in children, with stunted growthand anemia, is increasingly recognized in India [1–4]. In arecent study published from northern India, six of 25 (24%)short stature cases were diagnosed as celiac disease [2]. Wereport here the prevalence of immunoglobulin A tissue trans-glutaminase (IgA anti-tTG) antibody positivity among shortstature (<-3 SD) children and adolescents without gastrointes-tinal symptoms in the age group of 1–18 years.
A prospective hospital based study was done in the PediatricGastroenterology Section of Department of Pediatrics, betweenFebruary 2006 and October 2007. All children in the age groupof 1–18 years, having height less than -3 SD for their age andsex (as per CDC charts), were included if they had no
gastrointestinal symptoms suggestive of celiac disease. Anage and sex-matched control (children with upper respiratoryinfection, or those brought for vaccination with height morethan −2 SD and no symptoms suggestive of celiac disease) wastaken from the Pediatric outpatient services for every casewithin a week of enrolling the case. Previously diagnosed casesof celiac disease, cases with gastrointestinal symptoms andthose with height not less than −3 SD for their age and sexwere excluded. All children were northern Indian by ethnicity.
All subjects (study and control groups) underwent testingfor IgA anti-tTG assay (BindazymeTM Human Anti TissueTransglutaminase IgA EIA kit, The Binding Site Limited,Birmingham, United Kingdom). Total IgA was not done.Results were interpreted as per manufacturer’s instructions(negative was <4 U/mL, weak positive was 4–10 U/mL andpositive was >10 U/mL).
The sample size of 44 cases and controls was calculatedto be adequate if the anticipated prevalence was 1.7% [5],keeping estimate to be within 5 percentage points with 99%confidence interval and 90% power of the study. Fisher’sexact test was used to compare proportions.
One hundred and twelve patients with short stature withheight <−3 SD presented at the center; 61 of them had gastro-intestinal symptoms suggestive of celiac disease and wereexcluded. Fifty-one cases of short stature with height <−3 SDwithout gastrointestinal symptoms were included. Majority ofcases were in the age group of 1–6 years (47.1%); 33 of 51cases had weight z score less than −3 SD and 25 (49%) hadheight z score less than−4 SD. Themedian weight and height zscores of the cases were −3.13 and −3.99, respectively. Thecorresponding values in control subjects were −0.80 and−0.65, respectively. Six cases (11.8%) were IgA anti-tTGpositive, and all controls were seronegative. One of thesepatients had Marsh score IIIB on duodenal biopsy, 2 patientsrefused biopsy and 3 did not come back for further follow up.
F. Ahmad : S. M. AliDepartment of Pediatrics, J N Medical College,Aligarh Muslim University,Aligarh 202 002, India
S. Alam (*)Pediatric Gastroenterology Section, Department of Pediatrics,J N Medical College, Aligarh Muslim University,Aligarh 202 002, Indiae-mail: [email protected]
I. ShuklaDepartment of Microbiology, J N Medical College,Aligarh Muslim University,Aligarh 202 002, India
R. SherwaniDepartment of Pathology, J N Medical College,Aligarh Muslim University,Aligarh 202 002, India
Present Address:S. AlamPediatric Hepatology,Institute of Liver and Biliary Sciences, DI Vasant Kunj,New Delhi 110070, India
Indian J Gastroenterol (January–February 2012) 31(1):32–33DOI 10.1007/s12664-011-0160-2
Gluten withdrawal was done in the confirmed case and un-equivocal response in the form of increase in height was seenat 6 months follow up. On univariate analysis, hemoglobin<7 gm% (OR 10.25, 95% CI 1.53–68.62) in short staturechildren was associated with tTG positivity.
We found that 11.8% of 51 cases with short stature and withno gastrointestinal symptoms had IgA anti-tTG seropositivity.The estimated prevalence was higher than the prevalenceof CD reported (1.7% to 8.3%) in children with height<−2 SD, where the endocrine diseases were not excluded[5]. In this study we have studied only anti-tTG positiv-ity, and not the prevalence of celiac disease. The anti-tTG antibody is as good as anti-endomyseal antibody indetecting celiac seropositivity [6, 7], and this could be areason for a high prevalence in our cases.
Sharma et al. [2] found a prevalence of 24% CD amongshort stature children, however they did not mention whetherendocrine workup was done in their children. In studies wherethe endocrine causes had been ruled out, the prevalence of CD(4.7% to 59%) was higher [8, 9]. On univariate analysis wefound that only the presence of hemoglobin <7 gm% was therisk factor for celiac autoimmunity. We can conclude that 12%of the short stature with height <−3 SD who are severelyanemic and have no gastrointestinal symptoms could be sero-positive for IgA anti-tTG.
References
1. Puri AS, Garg S, Monga R, Tyagi P, Saraswat MK. Spectrum ofatypical celiac disease in North Indian children. Indian Pediatr.2004;41:822–7.
2. Sharma A, Poddar U, Yaccha SK. Time to recognise atypicalceliac disease in Indian children. Indian J Gastroenterol.2007;26:269–73.
3. Poddar U, Thapa BR, Singh K. Clinical features of celiac disease inindian children: are they different from the west? J Pediatr Gastro-enterol Nutr. 2006;43:313–7.
4. Ahmad F, Alam S, Shukla I, Sherwani R, Ali SM. Screeningchildren with severe short stature for celiac disease using tissuetransglutaminase. Indian J Pediatr. 2010;77:387–90.
5. Rossi TM, Albini CH, Kumar V. Incidence of celiac disease iden-tified by the presence of serum endomysial antibodies in childrenwith chronic diarrhea, short stature, or insulin-dependent diabetesmellitus. J Pediatr. 1993;123:262–4.
6. Bhadada SK, Bhansali A, Kochhar R, et al. Does every short staturechild need screening for celiac disease? J Gastroenterol Hepatol.2008;23 8 pt 2:e353–6.
7. Rostom A, Dube C, Cranney A, et al. The diagnostic accuracy ofserologic tests for celiac disease: a systematic review. Gastroenter-ology. 2005;128 Suppl 1:S38–46.
8. Bonamico M, Sciré G, Mariani P, et al. Short stature as the primarymanifestation of monosymptomatic celiac disease. J Pediatr Gastro-enterol Nutr. 1992;14:12–6.
9. Queiroz MS, Nery M, Cançado EL, Gianella-Neto D, Liberman B.Prevalence of celiac disease in Brazilian children of short stature.Braz J Med Biol Res. 2004;37:55–60.
Indian J Gastroenterol (January–February 2012) 31(1):32–33 33