Microscopic Agglutination and Polyacrylamide Gel Electrophoresis ...
If agglutination is observed, STOP the test and perform cell washing procedures before proceeding.
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RapidVet ® -H Blood Group Determination System for Identifying DEA 1.1 Positive and DEA 1.1 Negative Dogs dm s laboratories,inc.
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RapidVet ® -H Blood Group Determination System for Identifying DEA 1.1 Positive and DEA 1.1 Negative Dogs. - PowerPoint PPT Presentation
Transcript of If agglutination is observed, STOP the test and perform cell washing procedures before proceeding.
RapidVet®-HBlood Group Determination Systemfor Identifying DEA 1.1 Positive and
DEA 1.1 Negative Dogs
dmslaboratories,inc.
Kit contains:auto-agglutination saline screen cards, patient test cards, pipettes, stirrers, diluent, instructions, interpretation guideand report card.
Shelf-life: 24 months at room temperature
dmslaboratories,inc.
If agglutination is observed, STOP the test and perform
cell washing procedures before proceeding.
If auto-agglutination does not occur, proceed to the next step.
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Prior to typing, check for auto-agglutination using the card provided in the kit. This card is used for three patients and, after use, should be returned to the kit box.
Remove the Test Card from its plastic sleeve. Write the name/number of the dog and the
testing date on the card.
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Dispense 1 drop of diluent from the dropping bottle into each well.
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Aspirate a small amount of patient sample into a pipette and release 1 drop (50µl) into each well, taking care not to cross-contaminate the wells.
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Using a NEW stirrer and pressing downward with the flat portion of the stirrer, spread and mix the materials
within the entirety of the first well for about 10 seconds.
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Using a NEW stirrer, spread and mix the materials within the entirety of the second well
for about 10 seconds.
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Using a NEW stirrer, spread and mix the materials within the entirety of the third well
for about 10 seconds.dmslaboratories,inc.
Rock the card, using a transaxial motion, for 1 minute.
Be sure materials are mixing and
“rotating” within each individual well.
Be careful not to cross-contaminate.
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Read the results, noting whether agglutination has or has not occurred in the “Patient Test” well.
dmslaboratories,inc.DEA 1.1+ DEA 1.1
After the card has been read, take a digital photograph for a permanent record, or allow to dry and replace in its
plastic sleeve as a permanent record.
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