Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen...
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![Page 1: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/1.jpg)
Identifying Genes Underlying Spinal Motoneuron Specification
Karan NathaniSPUR 2011
Judith Eisen LabMentors: Liesl Van Ryswyk and Dr. Steve Seredick
![Page 2: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/2.jpg)
Overview
• Objective• Background• Summer Research• Conclusion• Future Directions
![Page 3: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/3.jpg)
Overview
• Objective• Background• Summer Research• Conclusion• Future Directions
![Page 4: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/4.jpg)
Motoneuron Specification
Goal: Understanding how motoneurons are specified
Types of Neurons
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Implications of This Study
• Treatment of several Motoneuron Diseases
Spinal Muscular Atrophy patient
![Page 6: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/6.jpg)
Why Zebrafish?
•Vertebrates•Transparent•Genetic•Tools
![Page 7: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/7.jpg)
Overview
• Objective• Background• Summer Research• Conclusion• Future Directions
![Page 8: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/8.jpg)
Road to Olfactomedin 1a
• Microarray screen
• Signaling Molecule
• Axon Pathfinding
![Page 9: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/9.jpg)
Olfactomedin 1a Background
Nakaya and Tomarev 2007
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Overview
• Objective• Background• Summer Research• Conclusion• Future Directions
![Page 11: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/11.jpg)
Hypothesis
• Olfactomedin 1a plays a role in specifying the formation of motoneurons.
Questions•Olfactomedin’s Role in Motoneuron Specification?•Olfactomedin’s Function?
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Methods
• RNA in situ hybridization• MO Injections• PCR
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Assessing Morpholinos•Titration•Concentrations: 50, 125, and 500 µM
50µM 125µM 500µM
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Overview
• Objective• Background• Summer Research• Conclusion• Future Directions
![Page 15: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/15.jpg)
Results
Uninjected Transgenic Fish MO injected transgenic fish
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ResultsConclusion: Olfactomedin 1a may have a role in axon pathfinding!
Uninjected transgenic fish MO injected transgenic fish
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Overview
• Objective• Background• Summer Research• Conclusion• Future Directions
![Page 18: Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.](https://reader034.fdocuments.net/reader034/viewer/2022052701/56649d955503460f94a7e832/html5/thumbnails/18.jpg)
Future Directions•Determine RNA Expression Pattern
•Further Studies of Protein Functions
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Acknowledgements
Eisen Lab• Judith Eisen• Liesl Van Ryswyk• Steve Seredick• Julia Ganz• Levi Simonson• Ellie Melancon• Dayna Lamb• Fish Facility
Spur Program•Peter O’Day•Lyndsey Goforth•Blakely Strand•Toby Fish•Interns
REU
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Thank you!
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Splice-blocking Morpholino