Hydra IIAF and Hydra IIAF Gold

The World’s Most Sensitive Mercury AnalyzersHg Analyzers

ICP Spectrometers

Hg EmissionMonitoring

Inorganic Standards

Don’t Settle for just any Mercury Analyzer......Go with the Experts in Mercury Analysis

...Go with Teledyne Leeman Labs

When the concentration of mercury needs to be accurately measured, laboratories all overthe world turn to Teledyne Leeman Labs for high performance solutions to their mercury

analysis challenges. Since introducing the first fully automated mercury analyzer in thelate 1980’s, Teledyne Leeman Labs has focused on advancing the analytical methodologiesas well as detection technologies for mercury. Now, well over two decades later, wehave both the applications expertise and the products needed to meet even the mostdemanding mercury analysis challenges. Today, our mercury products cover a broadrange of techniques including cold vapor atomic absorption (CVAA), cold vapor atomic

fluorescence (CVAF), direct solids analysis and mercury emissions monitoring. And, theyall comply with EPA and international methods for the determination of mercury.

Introducing the Hydra Series The Hydra Series is a family of fully automated instruments that address the analysis of liquids, solidsand gases.

The product line includes: Hydra IIAA, Hydra IIAF, Hydra-C and Hydra-CAppendix K

• Hydra IIAA – Where Performance and Productivity Meet

Hydra IIAA operates on the principle of cold vapor atomic absorption (CVAA) to provide theanalyst with a 1 ppt detection limit as well as compatibility with an extremely wide range ofsample matrices. Its high capacity autosampler, short analysis time, and over–range protectionsystem make the Hydra IIAA a powerful laboratory productivity tool.

• Hydra IIAF – Sub ppt Detection and Wide Dynamic Range

Hydra IIAF operates on the principle of cold vapor atomic fluorescence (CVAF) and is available in two configurations. The first is the core Hydra IIAF which provides a detectionlimit of 0.1 ppt and a dynamic range that extends to the high ppb level. For laboratories that require even lower limits of detection, the Hydra IIAF Gold includes a gold amalgamation system to preconcentrate mercury and yield a limit of detection well below 0.05 ppt. Bothconfigurations include a high capacity autosampler and over-range protection system toenhance productivity.

• Hydra-C – Mercury Analysis With No Sample Prep!

Hydra-C operates on the principle of thermal decomposition with atomic absorption detection to facilitate the direct analysis of solid or liquid samples. Hydra-C is designed toaddress the needs of analysts who want to use EPA method 7473 or who simply want todetermine mercury without having to digest their samples.

• Hydra-CAppendix K is designed to measure Hg emissions from coal-fired power plants, as wellas cement kilns and incinerators. It is compliant with 40 CFR, Part 75, Appendix K (SorbentTrap Analysis Method).

Teledyne Leeman Labs

offers both the technical

expertise and products

needed for your most

demanding mercury

analysis challenges

The Hydra II AF is capable of running samples in triplicate in about 1 minute, delivering a substantial improvement in productivity and in cost of analysis

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Instrument Hydra II AA Hydra II AF Hydra II AF Gold Hydra-C

Usage • Primarily for liquid samples • Determine Hg from low ppt

to ppm levels

• Primarily for liquid samples • Determine Hg from sub ppt

to high ppb levels

• Primarily for liquid samples • Determine Hg from 0.05 ppt

to high ppb levels

• Primarily for solid samples • Determine Hg in various

matrices without samplepreparation

Detection Limit 1 ppt 0.1 ppt <0.05 ppt 0.005 ng

Dynamic Range 1 ppt to 1 ppm 0.0001 to 250 ppb <0.00005 to 250 ppb 0.005 to 1000 ng (or up to 20,000 ng with the high range option)

Scientific Method Chemical reduction followed by cold vapor atomic absorption

Thermal decomposition followed by amalgamation and atomic absorption

Gas/Liquid Separation Leeman Labs High Performance gas/liquid separator provides exceptional sensitivities and recoveries, even for samples that foam during reduction. N.A.

Software Interface Hydra’s software provides ease-of-use and compatibility with other MS Windows® applications including Word®, Excel® and Access®1. It also permits custom report generation and data recalculation as well as connectivity to most LIMS systems.

Features • Dual beam detection system • 30 cm optical cell provides

exceptional sensitivity and stability, 2.5 cm optical cell provides access to high concentration Hg measurements

• High sample throughput for improved productivity and low cost of analysis

• Easy access sample introduction system

• Flow-through rinse minimizessample carryover, even at ultra-low levels

• High concentration protection system

• High capacity autosampler • Transition to direct solids

analysis (Hydra-C)

• Proprietary fluorescence cell• High sample throughput for

improved productivity and lower cost of analysis

• Counter-flow Nafion®

membrane dryer minimizeswater vapor-based scatter inthe fluorescence cell

• Flow-through rinse minimizessample carryover, even at ultra low levels

• High concentration protection system

• Proprietary fluorescence cell• High sample throughput for

improved productivity and lower cost of analysis

• Counter-flow Nafion®

membrane dryer minimizeswater vapor-based scatter inthe fluorescence cell

• Flow-through rinse minimizessample carryover, even at ultra low levels

• High concentration protection system

• Ultra trace analysis mode with gold amalgamation forimproved performance at sub-ppt levels

• Analysis in 5 minutes• Easy access maintenance• Green chemistry/ no chemicals

needed & no hazardous wasteproduced

• Applicable to both solid andliquid samples

• Transition to liquid analysis(Hydra IIAA)

EPA/ASTM Methods 245.1245.5245.67470A7471B

245.7 245.71631E

EPA 7473ASTM D-6722-01

European Standards EN 1483EN 13806

EN 13506 EN 13506EN 12338

Maintenance On-line audio visual help guides users through routine operation and maintenance. Predictive Maintenance ensures that Hydra series instruments consistently operate at peak performance.

Service A variety of post warranty service options are available to Hydra Series operators. Please inquire for additional details.1 Microsoft, Windows. Word, Excel, and Access are either registered trademarks or trademarks of Microsoft Corporation in the United State and/or other countries.Nafion is a registered trademark of E. I. duPont de Nemours

Chemical reduction followed by cold vaporatomic fluorescence

Chemical reduction followed by cold vaporatomic fluorescence

The Hydra Series at a Glance

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Hydra IIAF Gold

Fully compliant with EPA Methods 245.7 and 1631E as well as European Standards EN-13506 and EN-12338

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In an effort to routinely achieve sub ppt levels of detection for mercury, analysts around the globe are turning to cold vapor atomic fluorescence (CVAF) instrumentation from Teledyne Leeman Labs. We offer two CVAF – based instruments to meet your needs; the Hydra IIAF

and the Hydra IIAF Gold .

While the Hydra IIAF and Hydra IIAF Gold share a similar architecture (see Figures 1a and 1b) they are designed to address different methodologies and provide significantly different detection capabilities. The Hydra IIAF , with its direct fluorescence detection, provides adetection limit of 0.1 ppt, a dynamic range of over five orders of magnitude and exceptional stability. This system is designed to meet the requirements of EPA Method 245.7 and European Standard EN-13506. On the other hand, the Hydra IIAF Gold , with its ability to preconcentratemercury prior to analysis, is capable of achieving a detection limit well below 0.05 ppt and is fully compliant with EPA methods 1631E and 245.7as well as European Standards EN-13506 and EN-12338. Page 6 provides an overview of the benefits that are common to both the Hydra IIAF

and Hydra IIAF Gold. Page 7 provides an overview of the benefits that are unique to the Hydra IIAF Gold.

Introducing the World’s Most Sensitive Mercury Analyzers –the Hydra IIAF and Hydra IIAF Gold

Figure 1a. Hydra IIAF schematic diagram. Sample and reductant are pumped tothe gas/liquid separator where mercury is reduced to metallic vapor. Argon carries mercury vapor through the 2-stage dryer while liquid is pumped to waste. With theHydra IIAF the mercury vapor proceeds directly to the fluorescence cell for detection –in accordance with EPA Method 245.7 and EN-13506.

Figure 1b. Hydra IIAF Gold schematic diagram. The Hydra IIAF Gold uses the sameprocess as the Hydra IIAF for EPA Method 245.7 as well as preconcentrating Hg on a gold amalgamation system prior to detection – in accordance with EPA Method 1631E and EN-12338.

The Clean Water Act requires that individual states achieve “safe concentrations” for many

pollutants, including mercury in waters. The National Toxics rule defines the “safe” level for

mercury as low as 1.3 ppt, about 200 times lower than the reporting limit for EPA method 245.1

which uses Cold Vapor Atomic Absorption (CVAA).

Hydra IIAF Hydra IIAF Gold

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ACHIEVE LOWER DETECTION AND REPORTING LIMITS

Advanced Fluorescence Detection System. Both configurations ofthe Hydra IIAF incorporate a proprietary wide angle fluorescencedetector for the ultimate in sensitivity. This detector is designed withtangential gas flow for rapid equilibration and washout of Hg vapor; andfurther, it delivers a detection limit of 0.1 ppt without preconcentrationand well under 0.05 ppt with preconcentration.

DON’T GET DERAILED BY SAMPLES WITH HIGH MERCURY CONTENT

Over-Range Protection. One consideration important to commercialtesting labs is the potential for instrument contamination from sampleswith unexpectedly high concentrations of Hg. Such samples can wreakhavoc on instruments without an over–range protection system andcan lead to long and expensive decontamination procedures. This isespecially challenging for purge and trap instruments. Both the Hydra IIAF and Hydra IIAF Gold include over–range protection.

• With the Hydra IIAF , the instrument continually monitors the rateof change in signal for each new sample and then automaticallypredicts over-range conditions before system contamination can occur.

• With the Hydra IIAF Gold , the system performs a brief prescreenof each sample without preconcentration and then allows furtheranalysis only for those samples which are deemed safe (sampleswith concentrations below a user selectable set point).

Smart Rinse. Hydra IIAF also provides a smart rinse function whichhelps to ensure accurate measurements and is especially useful withthe over-range protection mode mentioned above.

RUN MORE SAMPLES WITH LESS LABOR

High Capacity Autosampler. In an effort to free your analyst forother tasks during periods of high sample loading, the Hydra IIAF

employs a high capacity autosampler that holds up to 270 samples. Its175 ml QC reservoirs ensure ample volume for continuing QC checks(e.g., CCV, CCB, OPR) during long sample runs.

RUN DIFFICULT SAMPLES WITHOUT COMPLICATIONS

High Performance Gas/Liquid Separator. Hydra II’s innovative,serpentine gas/liquid separator provides high throughput for routinesamples, superior analytical recoveries forcomplex or poorly digested samples andexceptional resistance to contaminationfrom difficult or foaming samples. Theresult is more robust operation, betterdata quality and increased productivity.

Filtered Autosampler Probe. Hydra’sfiltered sample probe excludes particulatesfrom the liquid stream to ensure the analyticalrobustness needed for long periods of unattended operation.

ELIMINATE WATER VAPOR FOR BETTER STABILITY

Method Compliant Vapor Dryer. Water vapor interferes with themeasurement of Hg by atomic fluorescence. For this reason a highperformance gas phase drying system is essential to instrument performance. EPA methods 245.7 and 1631E each recommend specificgas phase dryers; soda lime for Method 1631E and counterflowNafion® for 245.7. The soda lime dryer eliminates any condensationand protects the gold trap from haloacid vapors such as HCl or HBr.The membrane dryer then removes all traces of water vapor from the gas stream before it reaches the fluorescence cell. Hydra IIincorporates both dryers delivering method compliance, long-termstability, and higher sensitivity.

Benefits found in both the Hydra IIAF and Hydra IIAF Gold

Calibration plot in direct fluorescence mode from 1 ppt to 100 ppb demonstrating adynamic range in excess of 5 orders of magnitude.

A wide variety of commercially available sample cups are supported to achieve maximum productivity and to ensure compatibility with your lab’s digestion vessels.

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REPORT MERCURY AT CONCENTRATIONS FROM SUB PPT TOHIGH PPB LEVELS

With preconcentration the Hydra IIAF Gold achieves a MethodDetection Limit1 (MDL) of less than 0.05 ppt and easily satisfies theaccuracy requirement of the sub–ppt Report Limit1 standard inmethod 1631E; which is 0.5 ppt +/- 23%.

MEET LOW DETECTION LEVELS WITH LESS SAMPLE

The exceptional sensitivity of Hydra IIAF Gold allows it to meet theQC requirements of method 1631E using much less sample thanwas possible with earlier generation instruments. This means shorteranalysis times (2-3 minutes per sample) as well as the ability to fitmore samples (up to 180) into an automated run. The net result issuperior productivity for today’s busy laboratory.

COMPLY WITH BOTH EPA METHOD 245.7 AND 1631E

Not only is the Hydra IIAF Gold compliant with all the hardwarerequirements of Methods 1631E and 245.7, it is also compliantwith all the QC requirements of the methods. From its gold amalgamation trap and soda lime dryer to its software which automatically calculates “System Blanks1” and “CalibrationFactors1”, the Hydra IIAF Gold provides the user with an ideal platform for performing low level Hg determinations.

UNIQUE OVER-RANGE PROTECTION WITH PRECONCENTRATION

The Hydra IIAF Gold offers a fast, simple over-range protection system to ensure your productivity. When the purge and trapapproach is being used, over-range protection is accomplished byusing the fluorescence system without preconcentration to performa brief prescreen of each sample and then allowing further analysis only for those samples which are deemed safe (i.e. samples with concentrations below a user selectable set point).

ON-SITE CERTIFICATION ASSISTANCE FOR METHOD 1631E.

In addition to providing installation by a factory trained engineer,Leeman Labs offers an optional 1631E Certification Assistance program wherein an applications specialist will spend two days inyour lab to assist your personnel in the subtleties of meeting therequirements of EPA method 1631E (this includes site evaluation and recommendations).

Unique to the Hydra IIAF Gold

Gold amalgamation and fluorescence detection systems of the Hydra IIAF Gold

Calibration Details. The Calibration display provides a choice of several calibration curvefits including EPA’s required Calibration Factor (CF) algorithm. When the CF algorithm is chosen, the curve’squality control details appear including CF %RSD, System Blank concentration, and Report Limit recovery.

The figure above shows the stability of an on-going precision and recovery standard (OPR) collected over an 8-hour working day. Method 1631E requires a5 ppt OPR standard be analyzed with every batch of samples (20 maximum).

1 Method Detection Limit, Report Limit, System Blank and Calibration Factor are terms defined in EPA Method 1631E.

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The operating software for the Hydra II Series is a no-nonsense package designed to maximize the productivity of Hydra II and your laboratory.It automates all aspects of instrument operation as well as all autosampler functions. This is critical, especially for over-range protection. It alsohandles data storage, retrieval and reporting while providing powerful tools to assist with sample ID downloads and data uploads to LIMS systems.Hydra II is optimized for current EPA and EN methodologies, but with the versatility needed to handle any application. All calculations associatedwith EPA QC requirements, such as calibration factor curvefit and system blank verifications are handled directly within the software.

INSTRUMENT CONTROL

Hydra II is fully automated and all system parameters are saved with each analytical method. The Instrument Control screen shows a graphicaldisplay of the current analytical conditions and provides immediate control of all system functions including lamp, pump, gas flow, amalgamatorand autosampler.

Intuitive Software Maximizing the Productivity of the Hydra IIAF

QC AUTOMATION

QC Automation delivers unmatched flexibility for automated analysis. It defines both calibration and quality control locations aswell as defining when check standards appear during an automatedsequence. Its three tables allow selection of QC checks to be runbefore all samples (Initial), the QC checks to be inserted in the sample sequence after a specified number of samples (Continuous)and the checks to be run at the end of a sequence (Final). Uniqueactions are supported for each check in each table.

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SAMPLE SEQUENCING WITH “ON-THE-FLY” PROGRAMMING

For automated analysis the sample names are entered into thesample table. Sample names can be entered manually or bydownload from your LIMS System. Calibration standards andquality control checks established in the QC Automation sectionof the software are inserted automatically. While a sequence isrunning, new samples can be added.

REAL-TIME SIGNALS

During analysis, real-time signal displays provide direct comparisonof integrated measurements. These signals are saved with the sample data and are always available for review. Here is the signalfor a 1 ppt standard.

REPORTING FLEXIBILITY

When it comes time to generate a report, simply select thesample items, including calibration standards, quality controlchecks, and the details to be reported. There are about 30recorded items for each sample. The report displays theselected details from left to right in the order the items areselected. Once a Report Specification has been created, it canbe saved and recalled with a click of a button. At any timereports can be printed or exported for use by LIMS or thirdparty software (i.e. Word®, Excel®, etc.).

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CONTROL CHART

Control Charting provides a graphical display of QC resultsalong with calculated acceptance limits. This feature simplifiestracking the precision and stability of repeated quality control standards, such as CCVs or OPRs.

21 CFR COMPLIANCE TOOLS

21 CFR Compliance Tools to help satisfy 21 CFR 11 documentation requirements including detailed login information, a full audit trail, calculation validation from theraw data, and a permanent record of sample results andinstrument parameters.

SCHEDULED MAINTENANCE AND ON-LINE AUDIO/VISUAL HELP

To keep the Hydra II running at optimal performance, the software automatically displays suggested maintenance along with detailed helpdescribing proper procedures.

REMOTE DIAGNOSTICS

To assist with more complex issues, we have developed Leeman Link. Using the power of the internet, Leeman technicians run diagnosticson your instrument remotely. This enables us to review all the necessary information to answer questions quickly and efficiently. LeemanLink is available to all warranty and maintenance agreement customers.

Hydra IIAF Software

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WITH TELEDYNE LEEMAN LABS YOU GET MORE THAN JUST A SPECTROMETER

As you know, beyond the initial purchase price lies the true cost ofownership, and that’s where the Hydra Series shines. Why?Because Teledyne Leeman Labs has built these instruments to last.

Performance GuaranteeLike you, we expect our instruments to perform reliably, that’swhy we offer a unique comprehensive performance guarantee.Teledyne Leeman Labs warrants the Hydra Series of MercuryAnalyzers to:

1. Meet or exceed all functional and QC requirements of theEPA methods for which they were designed.

2. Provide greater than 95% up-time.3. Operate with fewer QC failures than competitive instruments.

After Sales SupportTelephone Support: Instrument malfunctions never occur at opportune times and your first line of defense is knowledgeabletelephone technical assistance. A technical support specialistcan provide you with information on instrument operation,trouble-shooting, applications, maintenance and help you withbasic repair. Most problems can be resolved over the phone.Our toll free hot line is available until 6:00 P.M. Eastern StandardTime (EST). Telephone assistance is provided by techniciansand support chemists with specialized training in emergencyrepair. Every call that comes into our Customer SupportCenter is entered into a computerized database so we cantrack the progress of your call until it is resolved.

On Site Support: On the rare occasion of instrument failure,our field service group is ready to provide the on-site assistance you need.

Service AgreementsA variety of optional service agreements are available dependingon your requirements, including:

Full Maintenance Agreement: A set rate comprehensive package that allows the user to budget for each instrumentunder the agreement.

Depot Maintenance Agreement: Includes all replacement partsand unlimited telephone technical support. It also includes ourdepot repair service that allows the user to return the unit toour factory repair center and ensures a guaranteed turn-around time of 5 days or less (from receipt of instrument).

Limited Maintenance Agreement: Allows the user to budget forcosts associated with replacement parts and technical support. This agreement is designed for the user who performs theirown maintenance, requires a fixed cost for replacement partsand may want access to our Technical Support Group to helpdiagnose a problem.

REAGENTS

Teledyne Leeman Labs prepares andvalidates standards and reagents forboth sample digestion and analysis.For low level mercury analysis,reagent purity is critical to controlling

contamination and obtaining accurateresults. With Teledyne Leeman Labs you

have an analytical partner, ready to providesupport for the chemistry involved in sample preparation as well asthe instrumentation to complete the analysis.

YOU’RE IN GOOD COMPANY

Are you getting the impression that we stand solidly behind ourHydra Series? We do! Our confidence comes from knowing that wehaven’t cut corners in the design, or testing of our products. Webuild our mercury analyzers to the most exacting specifications. Webuild them to do the job, and to do it right. Perhaps that’s whywe’ve become the industry leader in mercury analysis. It’s areputation that we’ve worked hard to earn, and that we workeven harder to maintain.

So what kind of companies and organizations rely on analyticalinstruments from Teledyne Leeman Labs? Our customers comefrom both the public and private sector. They are leaders in industry, government, and academia. Add to the list hundreds of cities, municipalities, and institutions of higher learning and you start to get a sense of the family of Teledyne Leeman Labs customers, and the impact that they have on our society.

We continue to invest heavily in research and development and look forward to providing our customers with additional products and technology advances in the years ahead.

Become a Teledyne Leeman Labs Customer......you’ll find yourself in good company

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6 Wentworth Drive • Hudson, NH 03051 U.S.A.Tel: 603.886.8400 • Fax: 603.886.9141LeemanLabsInfo@Teledyne.com www.TeledyneLeemanLabs.com

Made in the U.S.A. 1010

Principle of operation Atomic Fluorescence

Gases required Argon 15 psi

Power required 110/220 Vac, 50/60 Hz 100W

Dimensions 23" width x 19.5" depth x 23.5" height (584 mm W x 495 mm D x 597 mm H)

Weight 37 lbs (17 kg)

Operating ConditionsTemperature 15 – 30°C (60-86°F)

Relative humidity Maximum 80% non-condensing

Specifications