Anja Karine Ruud

Korn 2017

Hveteaksprikk

Hveteaksprikk (Parastagonospora nodorum – tidligere Septoria nodorum,

Stagonospora nodorum)

• Nekrotrof sopp• Viktigste

bladflekksykdom i norsk vårhvete

• Betydelige avlings- og kvalitetstap

• Resistens: Mange gener, moderat effekt

• Mål: Mer resistente sorter, forstå mekanismene bak

Sopp

Hveteblad

Nekrotrof effektor - SnTOX

Sensitivitetsgen - Snn

• Har disse interaksjonene effekt i felt?

• Kan vi bruke dem til å effektivisere foredlingen?

Sopp

Hveteblad

Nekrotrof effektor - SnTOX

Sensitivitetsgen - Snn

• Har disse interaksjonene effekt i felt?

• Kan vi bruke dem til å effektivisere foredlingen?

Metoder

Feltforsøk

Dusjvanning, naturlig inokulum (strå)

% sykdom

Aksskyting

Plantehøyde

Regresjon Resistens

Oppsummering viktige sorter

Sort Score2010-2016

Offisiellscore (% 2011-14)

SHA7/Milan -24.5

Demonstrant -9.3 15

Zebra -8.3 12

Mirakel -5.2 11

Krabat -1.7 16

Bjarne +7.2 24

Brakar +27.5Hveteblad

Oppsummering viktige sorter

Sort Score2010-2016

Offisiellscore (% 2011-14)

SnToxA SnTox3

SHA7/Milan -24.5 - +

Demonstrant -9.3 15 + -

Zebra -8.3 12 - -

Mirakel -5.2 11 + -

Krabat -1.7 16 + -

Bjarne +7.2 24 - -

Brakar +27.5 + -

Oppsummering viktige sorter

Sort Score2010-2016

Offisiellscore (% 2011-14)

SnToxA SnTox3 Inokulering(Snitt)

SHA7/Milan -24.5 - +1.6

Demonstrant -9.3 15 + -2.8

Zebra -8.3 12 - - 1.7

Mirakel -5.2 11 + - 2.9

Krabat -1.7 16 + - 1.6

Bjarne +7.2 24 - -3.4

Brakar +27.5 + -3.0

SnToxA-sensitivitet gir signifikant sykere planter i felt

SnTox3-sensitivitet hadde stor effekt i en populasjon

24 % av variasjon i felt

51 % i småplanteforsøk

wEx_c9301_154508180.00RFL_con5042_12330.25R875_c7582_680Ra_c68425_1406Ra_c68425_1419IACX7443Ku_c10387_272BS00066144_51

0.48

BS00091519_513.65K_c6784_7183.66BS00091518_513.79Snn33.96Bwc4838_584.05Exc_c47452_183GENE-3324_338

4.11

BS00068528_5133.17wPt-534640.16wPt-591440.47wKu_c35090_44349446wKu_c35090_44349517BS00033612_51K_c74960_427

42.50

Ra_c19198_137Exc_c58520_78

43.45

R875_c44613_8443.61barc21652.99BS00067028_5157.74BS00103625_5157.83Ku_c4349_1791BWrc55336_265

57.90

RFL_con2368_195857.92wKu_c32477_4208676058.19CAP7_c1403_7058.21IAAV219458.28Jagger_c6508_5158.36

5BS-SHA3/CBRDxNaxos

Kukri_c6784_718Excalibur_c47452_183BS00091519_51

1.36

BS00068528_5126.46Ra_c19198_13728.35Excalibur_c58520_7829.11wsnp_Ku_c35090_44349517wsnp_Ku_c35090_44349446BS00033612_51Kukri_c74960_427

29.62

wsnp_Ex_c8962_14947544RAC875_c44613_84

32.36

Tdurum_contig49841_61832.73wsnp_Ex_c26252_35497729Ex_c8962_467

33.02

BS00004406_5138.50Jagger_c6508_5140.06

5BS-Wang

wEx_c9301_154508180,0RFL_con5042_12330,3R875_c7582_680 Ku_c10387_272 BS00066144_510,5GENE-3324_338 Bwc4838_58 BS00091519_51 Exc_c47452_1833,7Snn35,4BS00068528_5135,4K_c20444_67738,0R875_c8661_692 Exc_c58520_7841,1BS00062986_5142,8wEx_c8962_14947544 R875_c44613_8444,4wEx_c26252_3549772944,5IAAV759444,6wKu_c35090_44349517 wKu_c35090_44349446 BS00033612_5145,2Td_con49841_61853,1Exc_c23426_21053,5Td_con25068_259 wEx_c2481_4632928 Exc_c92223_97 wExrc68091_66846454 BS00068082_5158,0IAAV425258,1Ex_c226_49958,2wEx_c5915_1037927758,4Ku_c4349_179158,7IAAV219458,9CAP7_c1403_7059,0wKu_c32477_42086760 RFL_con2368_195859,2Td_con25393_21859,4K_c19760_209159,7BWrc49783_6060,6Ex_c11331_121061,9Ku_c33217_323 R875_c45660_22862,2wEx_c39535_4680810562,4K_c40734_17962,5Bwc28958_17863,3wEx_c7988_13555376 wEx_c48257_5321753965,2wEx_c12909_20457660 BS00064891_5165,6wEx_c15858_24246431 RFL_con398_5165,7IAAV252667,2R875_c25020_128667,6R875_c31482_51368,4K_c17783_58 R875_c42518_5768,5wEx_c6391_11109898 K_c36789_230 Ex_c5632_77868,6BS00022086_5169,0Krc105540_177 BS00022662_5169,2CAP12_c1419_574 wEx_c53011_5639518570,0wRa_c5634_995201170,1Exc_c30346_54 BS00056147_51 BS00108062_5173,1BS00068390_5173,9wCAP11_c2439_125929975,1Ex_c66350_322 wKu_c14332_22613741 Exc_c49271_183 Td_con48658_794 Td_con48658_102275,3BS00040350_5175,4Td_con48658_80275,5wEx_c16704_25250247 Exc_c631_21975,6Bwc32495_258 Exc_c8082_478 BS00108020_5176,4wEx_c6548_1135552477,1Jagger_c9262_22377,6BS00064578_5177,7Excrc110349_30177,9Td_con12567_833 K_c11628_49878,5D_GA8KES402JRBN7_105 wEx_c709_139534784,8K_c2181_677 Ra_c35412_494 D_F5XZDLF01ELZ49_220 K_c2181_29184,9K_c805_32585,3BS00094480_5185,5Exc_c94356_529 Exc_c25661_121585,7R875_c12552_233 BS00009311_51 IAAV326893,5BS00021673_5193,8Ku_c11150_77393,9IACX7928 wEx_c222_43643294,0wEx_c28481_3760809294,1GENE-3397_53594,2Exc_c27820_112995,2D_GBB4FNX02HRJ2H_262 R875_s114930_80 wKu_c24804_3477382095,3K_c46126_851 R875_c52809_168 IACX9371 Ku_c5281_344 RFL_con4162_1757BS00011123_51 Bwc17735_194 BS00064661_51 K_c23752_659

95,4

K_c22631_462 tplb0060m15_117495,5IAAV5029 wRa_c24619_34168104 BS00067615_51 Excrc115624_180 K_c5281_991wEx_c9846_16236003 wRarc70152_67862271 K_c65301_127 tplb0060m15_1025

95,6

BS00064660_51 Td_con30483_335 R875_s116485_298 Bwc23569_43495,7BS00011931_5195,9Exc_c1371_454 K_c4054_494 CAP7_c319_16696,0Exc_c82761_5396,1BS00022040_5196,2BS00013829_5196,3R875rc87796_223 Jagger_c4318_7996,4K_c66814_10096,7R875_c53379_13596,9K_c22925_18597,6BS00011558_51 wEx_c25392_34658803 wKurc68793_67937879 Exc_c15931_497 R875_c14963_157997,8BS00106103_5198,0IACX7890 R875_c19099_308 BWrc61555_11598,3BS00022063_5198,4IAAV581198,9K_c57965_109 Exc_c29592_519 K_c7760_1210 wEx_c4031_729312599,3IACX9571 GENE-3310_73499,4Bwc7070_196 BS00068173_5199,6Excrc103557_293 BS00010837_5199,9BS00010033_51 GENE-3574_643 GENE-4297_86100,1K_c89764_273102,1GENE-0209_733 K_c22048_584102,5RFL_con3939_1276102,6Bwc18121_478 TA002629-0128 BS00070613_51102,7Exc_c36377_615 IAAV8195 R875_c10394_828 Bwc6633_476103,3R875_c7585_1097103,4Bwc47964_969103,6R875_c30584_75 Bwc6633_288103,8IAAV8929104,4IACX8282 BS00077667_51104,6R875_c35398_406105,5Bwc19443_131 Jagger_c5859_114 Td_con67535_391 Td_con75960_134 Td_con4864_148107,9wJDrc63083_40243538108,9BS00009335_51109,6

Sn4

0

5

10

15

5B

BS00064892_510,0wCAP12_c3056_14395671,4R875_c52560_4381,6wKu_c12886_20706969 Ku_c12886_12505,7CAP11_c1473_320 BS00061012_516,2BS00078460_516,4IAAV5150 IAAV12656,6Excrc68004_1826,7Ex_c9615_5746,8Td_con82438_136 Td_con82438_737,4R875_c63889_4867,5Td_con14075_6309,2Td_con20214_2799,5Td_con14075_3289,8Td_con20214_18110,1IACX1752210,6wPt-979613,2wPt-388314,0Td_con13011_24116,3Excrc109881_70120,4

BS00024786_5135,6RFL_con3447_1177 K_c67986_311 Ra_c8937_19136,1K_c12701_131 wEx_c2043_3829362 Ra_c17341_211937,3wPt-032137,7Td_con99143_20540,7

wPt-707654,8wPt-191754,9K_c7413_52255,5K_c2737_8956,4

CAP8_c3451_6573,9wmc60375,4K_c14516_22475,6wmc42275,7BS00069163_5177,0BS00006674_5177,3Td_con42093_117079,1Bwc5506_1551 BS00043869_5179,4BS00067486_5179,6K_c66328_222 wRa_c20189_29442564 BS00022959_5180,9K_c1406_490 K_c1406_275 Exc_c52798_19681,4R875_c14195_75882,4R875_c14195_115582,6R875_c62204_77288,8Bwc17095_23789,3Td_con45680_43489,6Td_con76289_153092,0wKurc113718_9623683092,1R875_c60161_243 Exc_c20307_65492,3K_c11269_205492,4Bwc1441_12892,5BS00103846_51102,7Td_con83837_251103,0Bwc30461_131103,1TA006231-0789103,7

7A

gwm4000,0Ku_c8929_2741,1Exc_c41298_4592,7TA002655-19642,9BS00111146_514,0wEx_c24376_336188645,0R875_c26328_755,2IACX39917,6Td_con41906_13858,6Exc_c26611_3259,4K_c18530_158410,4BS00021683_5111,2R875_c33179_24413,8Bwc20266_11515,4GENE-0293_28516,7BS00022550_5118,8gwm57325,3Bwc14812_82827,3wEx_c204_40054530,5RFL_con124_55832,7R875rc72877_15933,9wRa_c29350_3873400634,5wEx_c38995_4639350635,1BWrc50229_413 wCAP11rc6622_304445935,7BS00073602_5135,8Krc68742_543 R875_c5965_1153 wEx_c27373_3657827336,1wBE498662B_Ta_2_536,3RFL_con5098_1248 K_c61972_41936,8BS00095819_51 wEx_c25755_3501867437,3RFL_con1599_90637,4Exc_c22340_449 R875_c23724_182 K_c13040_323 Bwc25806_15737,5BS00068071_5137,7GENE-4720_64437,9BWrc66957_8438,2IAAV564638,3BS00031141_5138,6BS00110409_5138,7Td_con42813_93539,2R875_c5138_116 wRFLcon3054_2955094 tplb0046l06_716 BS00067540_5139,4BS00070399_5139,5Bwc9023_572 Td_con11727_274 R875_c7500_49641,5wPt-096341,8R875_c9715_679 Td_con50984_599 Td_con50984_55343,0Krc101980_52446,7Exc_c15029_35048,5BWrc63008_46849,5K_c22996_33450,6IAAV292151,7K_c39759_10252,3Td_con29238_37153,2barc17655,9wPt-1159857,6R875_c7123_170370,4Exc_c5700_52771,7K_c22495_55272,1wPt-299473,8BS00075332_5174,5Td_con41998_121379,1Krc68107_83381,0BS00022045_5181,1Exc_c29124_59882,3BS00079076_5183,4K_c14891_6484,6Td_con13930_25485,2wKu_c854_176806288,9wEx_c10500_1716395689,3wKu_c16295_2514903490,0D_GA8KES401CSA2F_12091,8tplb0021f14_170093,0Td_con28276_84994,0Td_con14821_75195,6BS00009995_51103,4BS00064368_51104,7Td_con5360_95105,1Td_con15734_221111,9Exc_c41736_124112,6wKu_c1455_2890228114,4wEx_c9017_15025720127,7R875_c41938_471128,6BS00010134_51129,9Td_con8719_370131,0wPt-3086132,8Bwc28058_232134,9R875_c54166_317135,9Exc_c16245_801136,4BWrc55035_124137,3BS00049961_51140,0wPt-0217141,6BS00075615_51143,6Krc79716_287144,1BS00005062_51145,2R875rc73236_354146,0BS00021666_51149,5K_c35975_593150,1Exc_c4484_985151,3wKu_c2648_5028958155,8Exc_c33267_538156,4R875_c31851_548157,4Td_con13459_543167,9R875_c14064_308169,1K_c80861_118170,7gwm1267a171,6wPt-0547172,8Td_con62213_156174,3tplb0045c05_154175,5Exc_c82295_669176,4K_c2348_2340178,0

7B

!

Konklusjoner

• Sensitivitet for SnToxA ga signifikant sykere planter i felt

• SnTox3-sensitivitet var viktig i én populasjon, men ikke i andre

• Flere interaksjoner involvert

• Genetiske markører er tilgjengelige for effektiv screening og seleksjon

• Sykdomsbildet i felt er komplekst

• Nødvendig med screeninger over flere år

• Småplanteforsøk er mer presise, kan validere og utfylle feltdata, men ikke erstatte

Takk for oppmerksomheten