Vitamins,Vitamins,VITAMIN A,VITAMIN D,VITAMIN E,VITAMIN K,Industrial production
HPLC & UPLC Method - INstruchemie...- Vitamin B6 = 80 nmol / liter - Vitamin B6 = 0.6 min. -...
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Vitamin B1 & B6 Combi – Determination HPLC & UPLC Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 1 van 12 VITAMIN B1 & B6 VITAMIN B1&B6 COMBI-DETERMINATION IN EDTA- OF HEPARINE WHOLE BLOOD HPLC & UPLC Method HPLC UPLC Retention times: Retention times: Vitamin B6 (PLP) - 1.8 min. Vitamin B6 (PLP) - 0.6 min. Internal Standard - 5.7 min. Internal Standard - 1.7 min. Vitamin B1 (TDP) - 7.9 min. Vitamin B1 (TDP) - 2.9 min. one mobile phase one wavelength (Ex, Em) isocratic method no incubation at 60 o C fluorescence detection 3 levels – Calibrator set 3 levels – Controle set Product name Product nr. Contents Package Vitamin B1 & B6 Reagent Set - HPLC 2996 50 – 70 tests Vitamin B1 & B6 Reagent Set - UPLC 3072 50 – 70 tests Vitamin B1 & B6 Direct Calibrator 3006 1 x 2 ml Vitamin B1 & B6 Calibrator Set 3111 9 x 1 ml Vitamin B1 & B6 Control Low Level 3018 10 x 2 ml Vitamin B1 & B6 Control Normal Level 3019 10 x 2 ml Vitamin B1 & B6 Control High Level 3020 10 x 2 ml Request for more information.
Transcript of HPLC & UPLC Method - INstruchemie...- Vitamin B6 = 80 nmol / liter - Vitamin B6 = 0.6 min. -...
Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 1 van 12
VITAMIN B1 & B6
VITAMIN B1&B6 COMBI-DETERMINATION IN EDTA- OF HEPARINE WHOLE BLOOD
HPLC & UPLC Method
HPLC UPLC
Retention times: Retention times:
Vitamin B6 (PLP) - 1.8 min. Vitamin B6 (PLP) - 0.6 min.
Internal Standard - 5.7 min. Internal Standard - 1.7 min.
Vitamin B1 (TDP) - 7.9 min. Vitamin B1 (TDP) - 2.9 min.
one mobile phase
one wavelength (Ex, Em)
isocratic method
no incubation at 60oC
fluorescence detection
3 levels – Calibrator set
3 levels – Controle set
Product name Product nr. Contents Package
Vitamin B1 & B6 Reagent Set - HPLC 2996 50 – 70 tests
Vitamin B1 & B6 Reagent Set - UPLC 3072 50 – 70 tests
Vitamin B1 & B6 Direct Calibrator 3006 1 x 2 ml
Vitamin B1 & B6 Calibrator Set 3111 9 x 1 ml
Vitamin B1 & B6 Control Low Level 3018 10 x 2 ml
Vitamin B1 & B6 Control Normal Level 3019 10 x 2 ml Vitamin B1 & B6 Control High Level 3020 10 x 2 ml
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 2 van 12
VITAMINE B1 & B6 COMBI - DETERMINATION IN EDTA of HEPARINE WHOLE BLOOD MONSTERMATERIAAL: Whole blood patient sample with EDTA or Heparine HPLC - ANALYTICAL CONDITIONS: Column : XBridge BEH C18 Column, 5 µm, 3.0 x 100 mm Flow Rate : 1.3 ml/min, Mobile Phase Temperature : 30 oC Detection : Fluorescence (ex. 370 nm, em. 455 nm) Injection volume : 35 µl – 40 µl Retention times : Vitamin B6 (PLP) ca. 1.8 min. : Internal Standard ca. 5.7 min. : Vitamin B1 (TDP) ca. 7.9 min. UPLC - ANALYTICAL CONDITION: Column : Acquity UPLC BEH C18 Column, 1.7 µm, 2.1 x 30 mm Flow Rate : 0.7 ml/min, Mobile Phase Temperature : 30 oC Detection : Fluorescence (ex. 370 nm, em. 455 nm) Injection volume : 10 µl Retention time : Vitamin B6 (PLP) ca. 0.6 min. : Internal Standard ca. 1.7 min. : Vitamin B1 (TDP) ca. 2.9 min. EXPECTED VALUES: TDP – B1 : 60 - 200 nmol/l PLP – B6 : 35 - 110 nmol/l PERFORMANCE: Linearity TDP – B1 : tot 17000 nmol/l Linearity PLP – B6 : tot 30000 nmol/l Within run precision TDP – B1:
N Sample material S.D. CV (%)
25 Whole blood patient samples with EDTA Mean = 173.5 nmol/l
4.44 2.56
Within run precision PLP – B6:
N Sample material S.D. CV (%)
25 Whole blood patient samples with EDTA Mean = 69.7 nmol/l
1.80 2.57
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 3 van 12
Calibration line Vitamine B6 & B1 Combi - Determination
-1- Vitamin B1 & B6 Calibrator Set (3111)
- Vitamin B1 Calibrator 1 = 50 nmol / liter - Vitamin B1 Calibrator 2 = 150 nmol / liter - Vitamin B1 Calibrator 3 = 250 nmol / liter
HPLC & UPLC - Method
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 4 van 12
Calibration line Vitamine B6 & B1 Combi - Determination
-2- Vitamin B1 & B6 Calibrator Set (3111)
- Vitamin B6 Calibrator 1 = 30 nmol / liter - Vitamin B6 Calibrator 2 = 80 nmol / liter - Vitamin B6 Calibrator 3 = 200 nmol / liter
HPLC & UPLC - Method
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 5 van 12
Chromatogram Vitamin B6 & B1 Combi – Determination
-3- Vitamin B1 & B6 Direct Calibrator (3006) Concentration: Retention times: - Vitamin B6 = 189 nmol / liter - Vitamin B6 = 1.8 min. - Internal Standard - Internal Standard = 5.7 min. - Vitamin B1 = 109 nmol / liter - Vitamin B1 = 7.9 min.
HPLC-Method
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 6 van 12
Chromatogram Vitamin B6 & B1 Combi - Determination
-4- Vitamin B1 & B6 Calibrator 1 (3108) Concentration: Retention times: - Vitamin B6 = 30 nmol / liter - Vitamin B6 = 1.8 min. - Internal Standard - Internal Standard = 5.7 min. - Vitamin B1 = 50 nmol / liter - Vitamin B1 = 7.9 min.
HPLC-Method
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 7 van 12
Chromatogram Vitamin B6 & B1 Combi - Determination
-5- Vitamin B1 & B6 Calibrator 2 (3109) Concentration: Retention times: - Vitamin B6 = 80 nmol / liter - Vitamin B6 = 1.8 min. - Internal Standard - Internal Standard = 5.7 min. - Vitamin B1 = 150 nmol / liter - Vitamin B1 = 7.9 min.
HPLC-Method
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 8 van 12
Chromatogram Vitamin B6 & B1 Combi - Determination
-6- Vitamin B1 & B6 Calibrator 3 (3110) Concentration: Retention times: - Vitamin B6 = 200 nmol / liter - Vitamin B6 = 1.8 min. - Internal Standard - Internal Standard = 5.6 min. - Vitamin B1 = 250 nmol / liter - Vitamin B1 = 7.9 min.
HPLC-Method
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 9 van 12
Chromatogram Vitamin B6 & B1 Combi – Determination
-7- Vitamin B1 & B6 Direct Calibrator (3006) Concentration: Retention times: - Vitamin B6 = 189 nmol / liter - Vitamin B6 = 0.6 min. - Internal Standard - Internal Standard = 1.7 min. - Vitamin B1 = 109 nmol / liter - Vitamin B1 = 2.9 min.
UPLC-Method
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 10 van 12
Chromatogram Vitamin B6 & B1 Combi - Determination
-8- Vitamin B1 & B6 Calibrator 1 (3108) Concentration: Retention times: - Vitamin B6 = 30 nmol / liter - Vitamin B6 = 0.6 min. - Internal Standard - Internal Standard = 1.7 min. - Vitamin B1 = 50 nmol / liter - Vitamin B1 = 2.9 min.
UPLC-Method
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 11 van 12
Chromatogram Vitamin B6 & B1 Combi - Determination
-9- Vitamin B1 & B6 Calibrator 2 (3109) Concentration: Retention times: - Vitamin B6 = 80 nmol / liter - Vitamin B6 = 0.6 min. - Internal Standard - Internal Standard = 1.7 min. - Vitamin B1 = 150 nmol / liter - Vitamin B1 = 2.9 min.
UPLC-Method
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Vitamin B1 & B6 Combi – Determination
HPLC & UPLC
Internet: www.instruchemie.nl E-mail: [email protected] Telephone: (+31)(0)596 – 634 831 Fax: (+31)(0)596 – 634 755 INstruendum-Vitamin B1&B6-HPLC-UPLC-Calibrator Set-2170628-1.FEN Pagina 12 van 12
Chromatogram Vitamin B6 & B1 Combi – Determination
-10- Vitamin B1 & B6 Calibrator 3 (3110) Concentration: Retention times: - Vitamin B6 = 200 nmol / liter - Vitamin B6 = 0.6 min. - Internal Standard - Internal Standard = 1.7 min. - Vitamin B1 = 250 nmol / liter - Vitamin B1 = 2.9 min.
UPLC-Method
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