HPLC SystemsHPLC Systems

Column Chromatography

HPLC ModesHPLC Modes

HPLC – System ComponentsHPLC – System Components

HPLC – Pump HeadHPLC – Pump Head

Piston HPLC Pump Head

Gradient Gradient HPLCHPLC

High Pressure

Low Pressure

Chromatography – Dead Volume MixingChromatography – Dead Volume Mixing

HPLCHPLCIonIon

SeparationSeparationModeMode

HPLC Solvents HPLC Solvents Mobile PhaseMobile Phase

HPLC – Solvents PropertiesHPLC – Solvents Properties

HPLC – Solvents GroupsHPLC – Solvents Groups

HPLC – Solvents Polarity for Reverse PhaseHPLC – Solvents Polarity for Reverse Phase

HPLC – Solvent Effect

HPLC – Effect of ModifierHPLC – Effect of Modifier

60:40 50:50 40:60

Acetonitrile : Water

HPLC – Gradient CurvesHPLC – Gradient Curves

HPLC ColumnsHPLC Columns

Mechanism of Mechanism of separation in separation in

different different forms of forms of HPLCHPLC

HPLC – Effect of Particles Size

HPLC – Particles SizeHPLC – Particles Size

HPLC – Porous and Solid PackingHPLC – Porous and Solid Packing

HPLC – Effect of Carbon Chain Length on SeparationHPLC – Effect of Carbon Chain Length on Separation

HPLC – Column Diameter and SeparationHPLC – Column Diameter and Separation

Size Exclusion HPLCSize Exclusion HPLC

HPLC DetectorsHPLC Detectors

HPLC – Diode Array Detector HPLC – Diode Array Detector

HPLC – UV/VIS Spectrophotometer

HPLC – Diode Array Detector OutputHPLC – Diode Array Detector Output

HPLC – UV/VIS DetectorHPLC – UV/VIS Detector

HPLC – Spectrometric Detector Flow Cell DesignHPLC – Spectrometric Detector Flow Cell Design

HPLC – UV Detector CellHPLC – UV Detector Cell

HPLC – Fluorescence DetectorHPLC – Fluorescence Detector

HPLC - ELSD

HPLC - Evaporative Light Scattering Detector

HPLC - DetectorsHPLC - Detectors

HPLC – Derivatization for DetectionHPLC – Derivatization for Detection

HPLC - ApplicationsHPLC - Applications