How to Screen out the High Productive Algae?

Qian Zhou10/16/2009

What is the criteria?• Daily biomass?• Nile Red Staining?• Biomass carbon and nitrogen percentage?• Daily lipids?• Daily carbon?• Daily reduced carbon?• (Batch)Total biomass in a relatively shot period?• (Batch)Total lipids in a relatively shot period?• Photosynthetic efficiency?• Nitrogen effects?

EV-B-6 EV-B-5 EV-B-1 EV-OR-2

EV-OR-5

EV-OR-1

EV-OR-6

EV-B-2 EV-B-7 EV-B-8 MCI PF-30

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

Series1

Figure1: daily biomass production of 12 algae stains

What we have done?

Method of lipid determination

• Gas Chromatography• Nile Red Staining• TD-NMR/ Regular NMR

TD-NMR

• Time domain NMR is an alternative method for lipid analysis.

• Highly accurate, no pretreatment needed• Could determine the lipid concentration in

real- time

basic principles of td-NMR

Plans for next step

• Determine the daily total biomass and lipids of 6~8 species out of 30 strains

• Determine the Phi of the 6 speciesⅡ• Determine the carbon dioxide utilization

of these species• Determine the nitrogen utilization of these

species• Compare FAME analysis with NMR data

Thank you!