How Cells Harvest Energy
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Transcript of How Cells Harvest Energy
How Cells Harvest Energy
Chapter 7
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Respiration
• Organisms can be classified based on how they obtain energy:
• autotrophs: are able to produce their own organic molecules through photosynthesis
• heterotrophs: live on organic compounds produced by other organisms
• All organisms use cellular respiration to extract energy from organic molecules.
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Respiration
• Respiration is a metabolic pathway of Redox Reactions
• Respiration typically oxidizes carbohydrates
• The type of molecule that is reduced determines the type of respiration
• The energy produced is in the form of ATP 3
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Respiration• Cellular respiration a metabolic pathway of redox reactions:
-oxidation – loss of electrons– dehydrogenations: loss of hydrogen e-’s
-reduction - gain of electrons– gain on hydrogen e-’s
• Oxidized molecules actually loose a hydrogen atom (1 electron, 1 proton)
• Both the protons and electrons are used by cellular respiration to produce ATP
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Respiration
• During respiration, high energy electrons are passed along chains of molecules - Electron Transport Chains
• Energy is released as molecules in the electron transport chains are oxidized
• The energy released is used to power the production of ATP
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Three Types of Respiration
• Respiration type is determined by the final electron acceptors:
1. Aerobic Respiration: final electron receptor is oxygen (O2)• Anaerobic Respiration: final electron acceptor is an inorganic molecule other than O2
• Fermentation: final electron acceptor is an organic molecule
Aerobic Respiration• Glucose contains chemical energy that
can be transferred and stored as ATP• Aerobic Respiration is a metabolic
pathway that oxidizes glucose and transfers the energy to produce ATP
• Oxygen is the final electron acceptor:• Recall:
C6H12O6 + 6 O2 6 H2O + 6 CO2 + EnergyGlucose Oxygen Water Carbon Dioxide
• The Energy is in the form of ATP
Aerobic Respiration
C6H12O6 + 6 O2 6 H2O + 6 CO2 + Energy
-Now-
C6H12O6 + 6O2 + 38 ADP + 38 P 6 H2O + 6CO2 + 38 ATP
Aerobic Respiration• Aerobic Respiration is a three stage
process:Stage 1: Glycolysis
Stage 2: The Krebs Cycle
Stage 3: Oxidative Phosphorylation
• Each of these stages produce ATP• At the end of all three stages, there is a
net gain of 38 ATP molecules (profit)– recall: cells are very efficient because of
enzymes
Stage 1: Glycolysis
• Glycolysis is a 10 step metabolic pathway that cleaves glucose
• Glyo-lysis = “splitting glucose”
• Glycolysis occurs in the cell’s cytoplasm– that’s where the enzymes for glycolysis are
located
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Stage 1: GlycolysisGlycolysis converts glucose to pyruvate
(pyruvic acid).
- a 10-step biochemical pathway
- occurs in the cytoplasm- 2 molecules of pyruvate are formed from
each glucose
- net production of 2 ATP molecules
-2 NADH produced by reduction of 2 NAD+
–recall NADH just is an electron carrier
–(see ch. 6)
Stage 1: Glycolysis• During Glycolysis, glucose (a 6 carbon molecule) is
chopped up into 2 Pyruvates (each pyruvate is a 3 carbon molecule)
• As glucose is cleaved, it is also being
oxidized - loosing electrons (hydrogens)
Figure 6_07
• Glucose is cut up into 2 Pyruvates in 10 steps
Figure 6_07
• 2 ATP must be invested during first two steps of glycolysis
• In step 1 ATP is used to phosphorylate glucose to make G-6-P• Phosphorylation destabilizes the glucose molecule so it can be cleaved• Phosphorylation reactions are carried out by enzymes known as Kinases - see chapter 6 and slide 38
Figure 6_07
• 2 ATP must be invested during first two steps of glycolysis
• In step 2, G-6-P is converted to F-6-P • This step is carried out by an isomerase enzyme• recall isomers from ch. 3 and slide 32
Figure 6_07
• 2 ATP must be invested during first two steps of glycolysis
•In step 3, 1 ATP is used to phosphorylate F-6-P to become F-1,6-bP • this step is carried out by another kinase
Figure 6_07
• In steps 4 and 5, the six-carbon molecule, F-1,3-bP is cleaved into 2 three-carbon molecules• G-3-P and Dihydroxyacetone phosphate (DHAP)• Dihydroxyacetone phosphate is immediately converted into another G-3-P
Figure 6_07
Very Important!
• Steps 6-10 occur twice for every glucose that enters glycolysis• because there are now two G-3P’s
Figure 6_07
• In step 6, G-3-P’s are oxidized• one NAD+ is reduced to produce one NADH
• Also in step 6, G-3-P’s are phosphorylated to produce 1,3-BPG
Figure 6_07
• One ATP is produced in step 7• 1,3-BPG is dephosphorylated to become 3-BPG• ADP is phosphorylated to ATP
Figure 6_07
• Steps 8 and 9 involve structural changes of 3-BPG to become Phosphoenolpyruvate
Figure 6_07
• In step 10, one more ATP is produced as Phosphoenolpyruvate is dephosphorylated to become Pyruvic Acid• another kinase
Fig. 7.6-1
Fig. 7.6-2
Fig. 7.6-3
Fig. 7.7-1
Fig. 7.7-2
Fig. 7.7-3
GlycolysisTotals
Per GlucoseCosts
– 2 ATPYield
– 2 NADH– 4 ATP
Net Gain from Glycolysis– 2 ATP– 2 NADH
Glucose
Pyruvate1 Pyruvate 2
2 ATP
4 ATP, 2 NADH
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NAD+, NADH
• During redox reactions, electrons carry energy from one molecule to another
• NAD+ is an electron carrier• NAD+ functions to carry electrons by carrying Hydrogen atoms
- NAD+ accepts 2 electrons and 1 proton to become NADH- The reaction is reversible
- NAD+ + 2e-’s + 1p+ NADH
NAD+
NAD+ Reduced to NADH
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FAD• FAD is very similar to NAD+
• It has the same function of collecting and carrying Hydrogen atoms from one molecule to another
• FAD can carry 2 Hydrogen atoms
• FAD is Reduced to FADH2
Stage 2: The Krebs Cycle• Also known as The Citric Acid Cycle
– citrate is the first molecule produced in this cycle
• The Krebs cycle is a metabolic pathway that further cleaves and oxidizes pyruvate
• The Krebs Cycle occurs in the cell membrane of Prokaryotic Cells and in the mitochondria of Eukaryotic Cells
• In mitochondria, a multienzyme complex called pyruvate dehydrogenase catalyzes the reaction
Stage 2: The Krebs Cycle
• The Krebs cycle is fueled with pyruvates from glycolysis– recall, there are 2 Pyruvates made from each Glucose, so there
are 2 Krebs Cycles for every glucose molecule
• Prep Step - before pyruvate enters the mitochondria for the Krebs cycle it is cleaved, oxidized and converted to become Acetyl-Coenzyme A (Acetyl-CoA)
Pyruvate
Acetyl-CoACO2
Krebs Cycle
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Prep Step: Pyruvate Oxidation
• Pyruvates are oxidized to form Acetyl-CoA– a CO2 moiety of pyruvate is exchanged for a
Coenzyme A(CoA) moiety
• The products of pyruvate oxidation include:- 1 CO2 - 1 NADH- 1 acetyl-CoA which consists of 2 carbons
from pyruvate attached to coenzyme A
• Acetyl-CoA proceeds to the Krebs cycle
Prep Step: Pyruvate Oxidation
• Prep Step: Pyruvates are converted to Acetyl-CoA with the release of CO2 in a preparation step
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Stage 2: The Krebs Cycle
• The Krebs cycle further oxidizes the acetyl group from pyruvate.
- Occurs in the matrix of the mitochondria
- Biochemical pathway of 5 steps
- First Step: Each Acetyl-CoA (2 carbon per molecule) is bonded to an Oxaloacetate (a 4 carbon molecule) to produce Citrate
acetyl group + oxaloacetate citrate
(2 carbons) (4 carbons) (6 carbons)
Stage 2: The Krebs Cycle
First Step of Krebs Cycle:
• Each Acetyl CoA (2 carbon per molecule) is bonded to an Oxaloacetate (a 4 carbon molecule)
• The new molecule made is Citrate (a 6 carbon molecule)
acetyl-CoA + oxaloacetate citrate(2 carbons) (4 carbons) (6 carbons)
Stage 2: The Krebs Cycle
• Citrate undergoes a five step cycle that builds additional ATPS
• During the Krebs Cycle additional NADH’s and FADH2’s are produced
• Citrate is eventually converted back into oxaloacetate and the cycle continues
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Fig. 7.12-2
In step 1, acetyl-CoA enters the mitochondria and combines with oxaloacetate to form citrate
Fig. 7.12-2
Citrate is further oxidized to produce 3 NADH and and FADH2
Fig. 7.12-2
2 CO2’s are
produced as Citrate is converted back into oxaloacetate
Fig. 7.12-2
The regenerated oxaloacetate is ready for another Acetyl-CoA and the Krebs cycle continues
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Stage 2: The Krebs Cycle
• The remaining steps of the Krebs cycle:
- release 2 molecules of CO2
- reduce 3 NAD+ to 3 NADH
- reduce 1 FAD (electron carrier) to FADH2
- produce 1 ATP
- regenerate oxaloacetate
The Krebs Cycle Totals(per pyruvate)
TotalsCosts• 2 ATP
Yields• 2 CO2
• 3 NADH• 1 FADH2
• 1 GTP (immediately converted to 1 ATP)
Glucose
Pyruvate1 Pyruvate 2
2 ATP
4 ATP, 2 NADH
2 ATP
1 ATP, 2 CO2
3 NADH, 1 FADH2
Pyruate converted to Acetyl Co-A
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Aerobic Respiration Review
• After glycolysis, pyruvate oxidation, and the Krebs cycle, one glucose has been completely cleaved and oxidized to produce:
- 6 CO2
- 4 ATP
- 10 NADH
- 2 FADH2
These electron carriers proceedto the electron transport chain for stage 3 of aerobic respiration
Glucose
Pyruvate1 Pyruvate 2
2 ATP
4 ATP, 2 NADH
2 ATP
2 ATP, CO2
8 NADH, 2 FADH2
Electron Transport Chain NADH, FADH234 ATP
Pyruate converted to Acetyl Co-A
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Stage 3: Electron Transport Chain
• The electron transport chain (ETC) is a series of membrane-bound electron carrier molecules called cytochromes
- embedded in the mitochondrial inner membrane
- electrons from NADH and FADH2 are transferred to cytochromes of the ETC
- each cytochrome transfers the electrons to the next cytochrome in the chain
Fig. 7.13a
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Stage 3: Electron Transport Chain
Energy from the Electrons
• As the electrons are transferred, some electron energy is released with each transfer
• This energy is used by the cytochromes to pump protons (H+) across the membrane from the matrix to the inner membrane space
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Stage 3: Electron Transport Chain
Energy from the Protons
• Electron energy is used by the cytochromes to pump protons (H+) across the membrane from the matrix to the inner membrane space
• A proton gradient is established– There are more protons on the
Stage 3: Electron Transport Chain• The cytochromes are channel proteins
and use the electron energy to pump protons (H+) across the inner mitochondrial membrane– Now there are more protons on the inside of
the membrane than the outside
• A proton gradient is established
• This proton gradient is potential energy that can be utilized to make more ATP’s– Recall diffusion: The protons want to equalize
their number on both sides of the membrane
Stage 3: Electron Transport Chain
Stage 3: Electron Transport Chain
• There are other channel proteins in the membrane known as ATP synthases
• ATP synthases provide a channel for the protons to diffuse through
• The rushing protons provides the energy for ATP synthase to phosphorylate ADP to ATP
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Stage 3: Electron Transport Chain
• In Aerobic Respiration, oxygen is the final molecule to receive the hydrogens as they are passed down the Electron Transport Chain
• The result is water: O2 + 4e- + 4H+ 2H2O
• Oxygen is reduced to water
Oxygen is the Final Electron Acceptor in Aerobic Respiration
The Electron Transport Chain
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Energy Yield of Respiration
• The ETC is very efficient and produces most of the ATP for cellular respiration (34 of the 38)
• Theoretical energy yields– 38 ATP per glucose for bacteria– 36 ATP per glucose for eukaryotes
• Actual energy yield– 30 ATP per glucose for eukaryotes– reduced yield is due to “leaky” inner membrane – reduced yield also due to chemiosis - the use of the
proton gradient for purposes other than ATP synthesis
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Glucose
Pyruvate1 Pyruvate 2
2 ATP
4 ATP, 2 NADH
2 ATP
2 ATP, CO2
8 NADH, 2 FADH2
Electron Transport Chain NADH, FADH230+ ATPAnd H2O
Pyruate converted to Acetyl Co-A
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Respiration Without O2
• Respiration occurs without O2 via either:
1. Anaerobic Respiration• use of inorganic molecules (other than O2) as the final electron acceptor
• Fermentation1. use of organic molecules as the final electron acceptor
Oxidation Without O2
• Anaerobic respiration produces fewer ATPs per glucose molecule compared to Aerobic Respiration
• Anaerobic respiration is much less efficient than aerobic respiration
• The exact amount of ATP production depends on the organism and the final electron acceptors that are used
Oxidation Without O2
• Anaerobic respiration is much less efficient than aerobic respiration– the ETC is bypassed– not all molecules are as readily reduced as O2 – other final electron acceptors may be reduced
to produce harmful products • fermentation of organic molecules produces acids
and alcohols
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Oxidation Without O2
• Anaerobic respiration by methanogens– methanogens reduce CO2 to regenerate NAD+
– CO2 is reduced to CH4 (methane)
• Anaerobic respiration by sulfur bacteria– inorganic sulphate (SO4) is reduced to
hydrogen sulfide (H2S)
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Oxidation Without O2
Fermentation reduces organic molecules in order to regenerate NAD+
1. Ethanol fermentation occurs in yeast
2. Lactic acid fermentation occurs in animal cells (especially muscles)
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Oxidation Without O2
Fermentation reduces organic molecules in order to regenerate NAD+
1. Ethanol fermentation• NADH reduces acetaldehydes to produce ethanol (an alcohol)
• CO2, ethanol, and NAD+ are produced
• Lactic acid fermentation1. NADH reduces pyruvate to produce lactic acid
Fig. 7.19-1
Fig. 7.19-2
Other Nutrients Serve as Energy Sources
• In addition to Glucose, many other molecules can be used by cells to produce energy through cellular respiration
• A variety of Carbohydrates, Lipids and Proteins can be catabolized for energy
• All of these must go through preparatory steps before they can enter into glycolysis
• For example: – Amino acids must go through a deamination process– Fatty Acids must go through beta oxidation
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Catabolism of Protein & FatCatabolism of proteins:
• Amino acids undergo deamination to remove the amino group (H2N-)- amino group removed as urea in the urine
• Remainder of the amino acid is converted to a molecule that enters glycolysis or the Krebs cycle- for example:
• amino acid alanine is converted to pyruvate to enter Krebs cycle
• animo acid aspartate is converted to oxaloacetate to enter Krebs cycle
Fig. 7.21
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Catabolism of Protein & Fat
• Catabolism of fats:– triglycerides are broken down to fatty acids
and glycerol– fatty acids are converted to acetyl groups by
β-oxidation – acetyl groups can enter the Krebs cycle
• The respiration of a 6-carbon fatty acid yields 20% more energy than glucose
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Preparatory Steps
Amino Acids
Lipids
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