Histology of Cartilage and Bone-SLIDE 21
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Transcript of Histology of Cartilage and Bone-SLIDE 21
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Cartilage consists of cells (chondrocytes) and an extensive extracellular matrixcomposed of fibers and ground substance.3 forms of cartilage have evolved, eachexhibiting variations in matrix composition:
1. Hyaline cartilage2. Elastic cartilage3. Fibrocartilage
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1. Hyaline cartilage: in the articular surfaces of themovable joints; the walls of larger respiratorypassages (nose, larynx, trachea, bronchi); theventral ends of ribs; and in the epyphysealplates of bones
2. Elastic cartilage: in the auricle of the ear; thewalls of the external auditory canals, theauditory tubes, the epiglottis, and the cuneiformcartilage in the larynx
3. Fibrocartilage: in the intervertebral disks; and inarticular menisci
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Perichondrium: outer layer (stratum fibrosum) type I collagen, inner layer (stratum
chondroblasticum) rich of cellsThe unit of cartilages: chondronChondrocyte: only one cell, or little groups ofcells, roundish shape, spherical nucleus
In hyaline cartilage: 2-4 chondrocytesIn elastic cartilage: 1-2 chondrocytesIn fibrous cartilage: 1 chondrocyte
LacunaPericellular matrixTerritorial/capsular matrixInterterritorial matrix
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Schematic representation of hyaline cartilage
territorial/capsular matrix
chondron
chondrocyteinterterritorialmatrix
lacuna
perichondrium
pericellular matrix
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Slide 7, Hyaline cartilage (HE), 10X
perichondriumstratum fibrosum
stratum chondroblasticum
interterritorial matrix
chondrons
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chondrocytes
territorial matrix
chondron
lacuna
Slide 7, Hyaline cartilage (HE), 40X
pericellular matrix
interterritorial matrix
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To identify the parts of hyaline cartilage:perichondriumchondronchondrocytepericellular matrixterritorial matrixinterterritorial matrixlacuna
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Slide 8, ear (orcein+H), 10X
perichondrium
perichondrium
chondrons
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elastic fibers
chondrocytes
chondron
Slide 8, ear (orcein+H), 40X
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To identify the components of the elasticcartilage:elastic fiberchondronchondrocyte
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Slide 9, Articular menisc (HE), 40X
collagen fibers
chondrocytes
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To identify the parts of the fibrocartilage:chondrocytescollagen fibers
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cancellous(spongy) bone
Inner circumferentiallamellae
compact bone
outer circumferential lamellaeHaversian system (osteon)
osteocyteHaversiancanal
Volkmann scanal
Schematic drawing of the bone
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Slide 10, Bone (ground section) 10X
Haversian canalVolkmann s canal
inner circumferential lamellae
outer circumferential lamellae
osteocytes
interstitial lamellae
osteon
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Haversian canal
osteocytes
Slide 10, Bone (ground section) 40X
osteon
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To recognize the following structures:osteonouter and inner circumferential lamellaeinterstitial lamellaeosteocyteVolkmann s canalHaversian canal
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Enchondralossification
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Slide 11, Enchondral ossification (HE) 4X
enchondral ossification
skeletal muscle
bone spicules
bone marrow
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bone marrow
bone spicule ossification zone
hypertrofic cartilage zone
proliferative zone
resting zone
Slide 11, Enchondral ossification (HE) 10X
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To identify:enchondral ossification with its zonesbone marrowbone spiculesskeletal muscleadipose tissue